LOCUS       IGI_P0621_pGL4_2        4849 bp ds-DNA     circular     13-APR-2017
DEFINITION  Firefly luciferase reporter vector pGL4.23[luc2/minP], complete
            sequence.
ACCESSION   DQ904455
VERSION     DQ904455.1  GI:115342874
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.23[luc2/minP]
  ORGANISM  Firefly luciferase reporter vector pGL4.23[luc2/minP] other
            sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     pGL4 Luciferase reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(1..19,586..636)
                     /note="multiple cloning region"
                     /label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(580..585)
                     /note="multiple cloning region"
                     /label=multiple cloning region(1)
                     /ApEinfo_label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      644..674
                     /regulatory_class="promoter"
                     /note="minimal promoter"
                     /label=minimal promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            707..2359
                     /gene="luc2"
                     /label=luc2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             707..2359
                     /gene="luc2"
                     /note="reporter gene"
                     /codon_start=1
                     /transl_table=11
                     /product="firefly luciferase"
                     /protein_id="ABI94439.1"
                     /db_xref="GI:115342875"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label=firefly luciferase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      2394..2615
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
                     /label=SV40 late poly(A) signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2683..2702
                     /note="reporter vector primer 4 binding region"
                     /label=reporter vector primer 4 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2940..2940
                     /note="ColE1-derived plasmid replication origin"
                     /label=ColE1-derived plasmid replication origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(3731..4591)
                     /gene="Amp(r)"
                     /label=Amp(r)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3731..4591)
                     /gene="Amp(r)"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="ABI94440.1"
                     /db_xref="GI:115342876"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=synthetic beta-lactamase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      4696..4849
                     /regulatory_class="polyA_signal_sequence"
                     /note="poly(A) signal/transcriptional pause site"
                     /label=poly(A) signal/transcriptional pause site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     4798..4817
                     /note="RVprimer3 binding region"
                     /label=RVprimer3 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    20..579
                     /label=CD69 CaRE3 plus strand
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    481..492
                     /label=Reference sequence has 13 T's, construct has 12
                     /ApEinfo_fwdcolor=#9e28ff
                     /ApEinfo_revcolor=#9e28ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggcctaactg gccggtaccA CCCCTTTCAA CCTCTTTATA AAACACAGAT GGCAATAATA
       61 TGTAACTTTC CATTTTAGGG AGACATTTTA TGTGTCTTCT TTCATAAATG ATATGTTAAA
      121 AAATTAAGTG ATATTTTTAG GGCAGAAATT ATCATTCACA TTTCATCGTG TAGCTGAGAA
      181 AATAGTATAG CCGAGGCTTA GAGAAATTAG TGCGGGGACA AGGTTGGCAA AAGTCTTTCA
      241 GGACACCAAA ACTCAATGCA GCTTTCCTTC CTGTGTGACC TTATATGTAG AATCCAGGGT
      301 GAGACGTCAG AAAGGAAGTA AAGATGAGGT AATATGAGCT AGATGCAAAG TGTGCAGAGA
      361 AGGTGGGGTA CTCTGTGCTT ATCAGACAGC TGCAGCAGCA GGATGCTTTT GGGTACATGT
      421 GAACAGGGAC CACAGACTTC CGACCTAAGA CAATTTCCGG AGACAAACTG CTCATCTCTC
      481 TTTTTTTTTT TTCCTTTCAG TGGGTGAATT AGGTTTCTGA ATGGCTTTTA ATCTTCTTGT
      541 GAATCAAAAC TTCAATTTAG AATACATCAT CCTCTCTCAg gtacctgagc tcgctagcct
      601 cgaggatatc aagatctggc ctcggcggcc aagcttagac actagagggt atataatgga
      661 agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa
      721 aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca
      781 gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc
      841 acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga
      901 agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag
      961 cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc
     1021 taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt
     1081 cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat
     1141 catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta
     1201 caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag
     1261 cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc
     1321 caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc
     1381 catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca
     1441 cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat
     1501 gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc
     1561 cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga
     1621 cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga
     1681 ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac
     1741 aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt
     1801 ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa
     1861 ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc
     1921 cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta
     1981 ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa
     2041 gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt
     2101 cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt
     2161 cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca
     2221 ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg
     2281 actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg
     2341 cggcaagatc gccgtgtaat aattctagag tcggggcggc cggccgcttc gagcagacat
     2401 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2461 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2521 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2581 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga
     2641 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     2701 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     2761 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3361 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3661 aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa atgctaaacc
     3721 actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc
     3781 gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt gagggcttac
     3841 catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc cccgatttgt
     3901 cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct actttgtccg
     3961 cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg ccagtgagta
     4021 gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg tcgttcggta
     4081 tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca cccatattat
     4141 gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg
     4201 tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg ccatccgtaa
     4261 gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc
     4321 gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat agcagtactt
     4381 tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg atcttgccgc
     4441 tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca gcatctttta
     4501 ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa
     4561 tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca
     4621 tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg
     4681 tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     4741 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     4801 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctct 
//
