LOCUS       IGI_P0624_pGL4_2        4989 bp ds-DNA     circular     15-JUN-2017
DEFINITION  Firefly luciferase reporter vector pGL4.23[luc2/minP], complete
            sequence.
ACCESSION   DQ904455
VERSION     DQ904455.1  GI:115342874
KEYWORDS    .
SOURCE      Firefly luciferase reporter vector pGL4.23[luc2/minP]
  ORGANISM  Firefly luciferase reporter vector pGL4.23[luc2/minP] other
            sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     pGL4 Luciferase reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Swanson,B., Kopish,K. and McNamara,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2006) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    join(1..19,726..776)
                     /note="multiple cloning region"
                     /label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(720..725)
                     /note="multiple cloning region"
                     /label=multiple cloning region(1)
                     /ApEinfo_label=multiple cloning region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      784..814
                     /regulatory_class="promoter"
                     /note="minimal promoter"
                     /label=minimal promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            847..2499
                     /gene="luc2"
                     /label=luc2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             847..2499
                     /gene="luc2"
                     /note="reporter gene"
                     /codon_start=1
                     /transl_table=11
                     /product="firefly luciferase"
                     /protein_id="ABI94439.1"
                     /db_xref="GI:115342875"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label=firefly luciferase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      2534..2755
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
                     /label=SV40 late poly(A) signal
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2823..2842
                     /note="reporter vector primer 4 binding region"
                     /label=reporter vector primer 4 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      3080..3080
                     /note="ColE1-derived plasmid replication origin"
                     /label=ColE1-derived plasmid replication origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(3871..4731)
                     /gene="Amp(r)"
                     /label=Amp(r)
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3871..4731)
                     /gene="Amp(r)"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="ABI94440.1"
                     /db_xref="GI:115342876"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=synthetic beta-lactamase
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     regulatory      4836..4989
                     /regulatory_class="polyA_signal_sequence"
                     /note="poly(A) signal/transcriptional pause site"
                     /label=poly(A) signal/transcriptional pause site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     4938..4957
                     /note="RVprimer3 binding region"
                     /label=RVprimer3 binding region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(join(20..230,232..481,483..719))
                     /label=IL2RA CaRE2 minus strand
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    482..482
                     /label=rs660245
                     /ApEinfo_fwdcolor=#9e28ff
                     /ApEinfo_revcolor=#9e28ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    231..231
                     /label=A>G mutation at chr10:6117603
                     /ApEinfo_fwdcolor=#9e28ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggcctaactg gccggtaccA CAGATTCCAG CTGTCCAGGA TTTCAACATC CCAGGTAGTC
       61 ACCTATTCAC ACCTGAGTGT ATGCAACTAA GCTGTGCGCT GAATACTTTG TGCAAAAACG
      121 CCCAAAGGGG ACTGTGGGGT GGAGGGGACT GGGGGGTGGA GGGGACTGCG GGGTGGAGGA
      181 GACTGTGGGG TGGAGGAGAC TGGGGGGTGG AGGGGACTGC GGGGTGGAGG gGACTGTGGG
      241 GTGGAGGGGA CTGTGGGGTG GAGGGGACTG TGGGGTGGAT GACTCCCAAG TGCACCAGGC
      301 AGGTGACTGG TGAATATGCT ACAGTGCTCG GAAATATACC AACATACCCC CCACACATAT
      361 TTATTTTCAG GAAGGGCTAA CTGTTGCAAT TTCACCCCCC TTTCTCCCAG AAGAATAGAT
      421 CGTACACCGC TCGGTTGAAA TGAAGTAGTT TCATCAGTTC TAACAAATGC GCCACGCTGG
      481 TcGGGGTGTT GATAGTTGGG GCGCTGTGCG GGTGTGAGGG CAGGAGGCAC AGGGGAACTC
      541 TCTCTCCCTT CCACTCGAGT TTGCTGTGCG TCTAAAACTG CTCTAAAAAC AAAGTCTATT
      601 TGAAAAGAAA CCTCGCAGTT GCAGTCCCCA CAACTGCCTC CCGCTGCTGC CCCATACCCT
      661 CTGCTCCACT TTGGAGCACA GCCTCTCAGA TGAGCTGTTG GTCCAGCACC TCTACAGCAg
      721 gtacctgagc tcgctagcct cgaggatatc aagatctggc ctcggcggcc aagcttagac
      781 actagagggt atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa
      841 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
      901 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
      961 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     1021 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     1081 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     1141 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     1201 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     1261 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     1321 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     1381 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     1441 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     1501 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     1561 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     1621 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     1681 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     1741 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     1801 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     1861 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     1921 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     1981 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     2041 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     2101 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     2161 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     2221 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     2281 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     2341 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     2401 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     2461 ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag tcggggcggc
     2521 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2581 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2641 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     2701 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     2761 gataaggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     2821 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     2881 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3841 gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat
     3901 ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg tgtagatcac
     3961 tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc gagagccgcg
     4021 ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg agcgaagaag
     4081 tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg atgctagagt
     4141 aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg gcatcgtggt
     4201 atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt caagccgggt
     4261 cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt
     4321 cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac acaattctct
     4381 taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt
     4441 ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac gggacaacac
     4501 cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt cggggcggaa
     4561 agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc ttgcacccag
     4621 ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca
     4681 aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca tactcgtcct
     4741 ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag gataagtaag
     4801 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
     4861 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     4921 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     4981 catttctct     
//
