LOCUS IGI_P0346_pGL4_2 4766 bp ds-DNA circular 13-APR-2017 DEFINITION Firefly luciferase reporter vector pGL4.23[luc2/minP], complete sequence. ACCESSION DQ904455 VERSION DQ904455.1 GI:115342874 KEYWORDS . SOURCE Firefly luciferase reporter vector pGL4.23[luc2/minP] ORGANISM Firefly luciferase reporter vector pGL4.23[luc2/minP] other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4283) AUTHORS Swanson,B., Kopish,K. and McNamara,B. TITLE pGL4 Luciferase reporter Vectors Technical Manual, TM259 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4283) AUTHORS Swanson,B., Kopish,K. and McNamara,B. TITLE Direct Submission JOURNAL Submitted (16-AUG-2006) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature join(1..35,535..553) /note="multiple cloning region" /label=multiple cloning region /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 regulatory 561..591 /regulatory_class="promoter" /note="minimal promoter" /label=minimal promoter /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 gene 624..2276 /gene="luc2" /label=luc2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 624..2276 /gene="luc2" /note="reporter gene" /codon_start=1 /transl_table=11 /product="firefly luciferase" /protein_id="ABI94439.1" /db_xref="GI:115342875" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" /label=firefly luciferase /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 regulatory 2311..2532 /regulatory_class="polyA_signal_sequence" /note="SV40 late poly(A) signal" /label=SV40 late poly(A) signal /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 2600..2619 /note="reporter vector primer 4 binding region" /label=reporter vector primer 4 binding region /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 rep_origin 2857..2857 /note="ColE1-derived plasmid replication origin" /label=ColE1-derived plasmid replication origin /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 gene complement(3648..4508) /gene="Amp(r)" /label=Amp(r) /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS complement(3648..4508) /gene="Amp(r)" /codon_start=1 /transl_table=11 /product="synthetic beta-lactamase" /protein_id="ABI94440.1" /db_xref="GI:115342876" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" /label=synthetic beta-lactamase /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 regulatory 4613..4766 /regulatory_class="polyA_signal_sequence" /note="poly(A) signal/transcriptional pause site" /label=poly(A) signal/transcriptional pause site /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 primer_bind 4715..4734 /note="RVprimer3 binding region" /label=RVprimer3 binding region /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 34..533 /label=IL2RA CaRE4 plus strand /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 109..455 /label=Conserved core /ApEinfo_fwdcolor=#2c9c57 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 218..218 /label=rs61839660C>T /ApEinfo_fwdcolor=#9e28ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gccTCCCTCT TCCTCCTGTA CCCTCAACCC 61 ATCAGCTGCC TGTACTGCTG TAGCAACAAC TTCTCTCTGA AAACCTTCCC AGGGGAGAAA 121 ACGCGCCCCC CCTCACGCCT CAGAGGCACA CACCTATCCT AGCCTTTGTT TTTTTATTGC 181 TGAGAGTACA GAAAGCAGCG GCTTCTGAAG GAGGTATTTA TTTTGGTCCC AAACAGAAAA 241 GAGTGGGTGA TTCTGTGGGC AGAGGTGGCA ATTCCTGAAT GTGGTATGGT GACTCACGCC 301 CGGAGGTGCT CAGAATTTCC TCTGTTTTGT GGCTGCTGGG GCAGGTGCGA CACTGCCGCT 361 TTCTCTAAAG ATGCCCCCAA GCTGCTCTGG GGGTAGAAGG AAGGCTGCAG GGAACAGCAG 421 GACCCGGAGA ACCCAGACCC CAAGGACCAG GTGGTCCTTA AGCCATCCTG CAGGAGGCCG 481 GCTGCTCTAG CAGGTTGAGG AGTGCCACGC GGCTCTGTGG GTACCGCCTC ACTtggcctc 541 ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca gcttggcaat 601 ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag ggcccagcgc 661 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct 721 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct 781 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga 841 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt 901 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg 961 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc 1021 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg 1081 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc 1141 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg 1201 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc 1261 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc 1321 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc 1381 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat 1441 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta 1501 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg 1561 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc 1621 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc 1681 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg 1741 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc 1801 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg 1861 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct 1921 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg 1981 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc 2041 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca 2101 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc 2161 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc 2221 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaataat 2281 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg 2341 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 2401 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 2461 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct 2521 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca 2581 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc 2641 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga 2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3301 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 3601 gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg 3661 atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt ggcctgactc 3721 cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag cgcagcaatg 3781 atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca gccagcaggg 3841 agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc tatgagctgc 3901 tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt tgtggccatt 3961 gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc 4021 cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt cagctcctta 4081 gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca 4141 gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt gaccggcgag 4201 tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc ttgcccggcg 4261 tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat catcgggaat 4321 cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag ttcgatatag 4381 cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt 4441 gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg 4501 atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc 4561 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat 4621 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata 4681 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc 4741 aagtgcaggt gccagaacat ttctct //