LOCUS       Exported                9113 bp ds-DNA     circular SYN 13-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p2CT-His-MBP-Lne_Cas13a_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 13, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(139..167)
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    209..1069
                     /label=AMP resistance beta-lactimase
                     /note="AMP resistance beta-lactimase"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     CDS             407..1066
                     /label=AmpR
                     /note="AmpR"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     rep_origin      1164..1846
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="ApEinfo_revcolor=#f8d3a9
                     ApEinfo_fwdcolor=#f8d3a9"
     misc_feature    complement(1276..1385)
                     /label=RNA1-for
                     /note="RNA1-for"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    complement(1824..1833)
                     /label=ColE1 ORI
                     /note="ColE1 ORI"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    complement(2257..2482)
                     /label=ROP/ROM
                     /note="ROP/ROM"
                     /note="ApEinfo_revcolor=#ff9ccd
                     ApEinfo_fwdcolor=#ff9ccd"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    4147..5349
                     /label=MBP ORF
                     /note="MBP ORF"
                     /note="ApEinfo_revcolor=#84b0dc
                     ApEinfo_fwdcolor=#84b0dc"
     misc_feature    4159..4176
                     /label=His6 tag
                     /note="His6 tag"
                     /note="ApEinfo_revcolor=#5757ff
                     ApEinfo_fwdcolor=#5757ff"
     misc_feature    5325..7074
                     /label=WP_036091002.1-gB1
                     /note="WP_036091002.1-gB1"
                     /note="ApEinfo_revcolor=#75c6a9
                     ApEinfo_fwdcolor=#75c6a9"
     misc_feature    5350..5364
                     /label=Lic_v1_For
                     /note="Lic_v1_For"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    5368..8520
                     /label=WP_036091002.1
                     /note="WP_036091002.1"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    7035..8567
                     /label=WP_036091002.1-gB2
                     /note="WP_036091002.1-gB2"
                     /note="ApEinfo_revcolor=#c6c9d1
                     ApEinfo_fwdcolor=#c6c9d1"
     misc_feature    8528..8542
                     /label=Lic_v1_rev
                     /note="Lic_v1_rev"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    8649..8667
                     /label=T7 terminator primer
                     /note="T7 terminator primer"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    8670..8710
                     /label=T7 terminator
                     /note="T7 terminator"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgcaatg aaaattacca agatgcgcgt ggatggtcgt
     5401 acgattgtga tggaacgtac ttcgaaagaa ggacagctgg gctatgaagg catcgacggg
     5461 aacaaaacga ctgagattat tttcgataaa aagaaggaat ctttttacaa gagcattttg
     5521 aacaaaaccg tccgcaaacc ggacgagaaa gagaaaaatc gccgcaaaca agcaattaac
     5581 aaagcaatta ataaggagat cacggagtta atgctggccg tgcttcatca agaggtgcct
     5641 tcgcaaaaac tgcacaactt aaagtcactt aacacggagt cgttaacgaa attattcaag
     5701 cctaagttcc agaatatgat tagttaccct ccttccaaag gcgctgagca tgtgcagttt
     5761 tgcttaacag atattgcggt tccggcaatc cgtgatcttg atgagattaa gccggattgg
     5821 ggtatctttt ttgaaaaact taagccttac accgattggg ctgagagcta catccattac
     5881 aaacaaacaa ccattcaaaa gtcgattgag cagaacaaga ttcaatcacc ggactccccg
     5941 cgcaagttgg tattgcaaaa atatgtcact gccttcctga atggtgagcc ccttggatta
     6001 gatttggtcg ctaagaagta taaattagcc gacctggctg agagtttcaa actggtcgac
     6061 cttaatgagg ataaatcagc gaattacaaa attaaagcct gtcttcagca gcaccagcgt
     6121 aacatcttgg atgagttaaa ggaagatcca gaacttaacc aatatggtat tgaagtcaaa
     6181 aagtacattc agcgttactt ccctattaaa cgtgcaccaa atcgctccaa acatgcacgt
     6241 gcagacttct taaagaaaga attaattgaa tcgacagtgg aacaacaatt caaaaacgcg
     6301 gtctatcact acgtattgga acagggaaaa atggaagcat acgaacttac tgaccctaaa
     6361 actaaagatc ttcaggacat tcgcagcgga gaagcattct catttaaatt catcaacgcg
     6421 tgcgcattcg caagcaacaa cttaaagatg attcttaatc cggagtgcga aaaagacatt
     6481 ctgggcaaag gcaacttcaa aaaaaacctg cccaattcaa cgacgcgttc tgacgtcgta
     6541 aaaaaaatga ttcctttttt cagtgacgaa ctgcaaaacg tgaattttga cgaggccatc
     6601 tgggccatcc gtggctcaat ccaacaaatc cgtaatgaag tgtatcactg taagaagcat
     6661 tcttggaaat cgatccttaa gattaaaggc tttgaatttg aaccgaataa catgaaatac
     6721 gcagattccg atatgcaaaa gcttatggac aaagacatcg ccaagattcc ggagtttatt
     6781 gaggaaaaat tgaaatcatc aggcgtagtg cgtttttacc gtcatgacga gctgcaaagc
     6841 atttgggaaa tgaagcaagg tttctcgctg cttactacga acgcgccgtt cgtgccctcg
     6901 tttaaacgcg tgtatgcgaa ggggcatgat tatcaaacaa gcaagaaccg ctactacaat
     6961 ctggacctta caaccttcga catcttggaa tacggagaag aagactttcg tgctcgctat
     7021 ttcttaacta agcttgtata ctatcaacag ttcatgcctt ggtttactgc cgataataac
     7081 gcctttcgcg acgcagccaa ttttgtgtta cgcttaaata aaaatcgtca gcaggatgcg
     7141 aaagcattca tcaacatccg cgaagttgaa gagggagaga tgccgcgcga ttacatgggt
     7201 tatgttcagg gccaaatcgc gatccatgaa gattcgattg aggatactcc taaccatttc
     7261 gaaaagttta tcagtcaagt ttttattaaa ggttttgacc gtcacatgcg ttccgcgaat
     7321 ttgaaattca tcaagaatcc acgcaatcaa ggccttgaac agtcggagat tgaagaaatg
     7381 tcgttcgaca tcaaggtaga gccttccttt ctgaaaaaca aggatgacta cattgctttt
     7441 tggatttttt gtaaaatgct ggatgcccgc cacttatcag aattacgtaa tgagatgatt
     7501 aagtatgatg gacacttgac gggagaacaa gagatcattg gtttagcgtt actgggtgtt
     7561 gacagccgcg agaacgactg gaagcagttt ttttcctcag agcgcgaata tgagaaaatc
     7621 atgaagggct acgtcgtgga agaactttat cagcgcgagc catatcgtca gtcagatggg
     7681 aagacgccta tcttattccg tggcgtggaa caggcacgca aatatggtac agaaaccgtg
     7741 attcaacgcc tttttgacgc caatcccgaa tttaaggtca gcaaatgtaa cttagccgag
     7801 tgggagcgcc aaaaagagac cattgaagag acaatcaaac gccgcaagga attgcacaat
     7861 gaatgggcca agaaccctaa gaaaccgcag aacaatgcgt tcttcaaaga gtataaagaa
     7921 tgctgcgatg ctatcgatgc ctacaattgg cataagaata aaaccacatt ggcctatgta
     7981 aatgaactgc atcacttact tatcgaaatc ttggggcgct atgttggcta tgttgcgatc
     8041 gcggatcgcg atttccagtg tatggccaat caatacttta agcactcagg cattaccgaa
     8101 cgtgtcgaat actggggaga caatcgcctt aaaagcatta aaaagttgga cacttttctg
     8161 aagaaagaag gtctttttgt aagtgagaag aacgcccgca accatattgc gcatttaaac
     8221 tacctgtcat taaagtctga gtgtactctt ttataccttt ccgagcgctt acgtgagatc
     8281 ttcaaatacg atcgtaagct taaaaacgca gtttcaaaat ccttaattga cattcttgat
     8341 cgccatggga tgtcagttgt ctttgccaac cttaaagaaa acaaacaccg cctggttatc
     8401 aagagtttag aaccaaagaa acttcgtcat ttaggtggga agaaaatcga tggcggatac
     8461 attgagacta accaagtaag cgaggagtat tgcggaatcg tgaaacgttt gcttgaaatg
     8521 taataacatt ggaagtggat aacggatccg cgatcgcggc gcgccacctg gtggccggcc
     8581 ggtaccacgc gtgcgcgctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     8641 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     8701 gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca
     8761 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     8821 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     8881 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     8941 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     9001 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     9061 gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
//
