LOCUS       Exported               10271 bp ds-DNA     circular SYN 13-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p2CT-His-MBP-Lba_Cas13a_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 13, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(139..167)
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    209..1069
                     /label=AMP resistance beta-lactimase
                     /note="AMP resistance beta-lactimase"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     CDS             407..1066
                     /label=AmpR
                     /note="AmpR"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     rep_origin      1164..1846
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="ApEinfo_revcolor=#f8d3a9
                     ApEinfo_fwdcolor=#f8d3a9"
     misc_feature    complement(1276..1385)
                     /label=RNA1-for
                     /note="RNA1-for"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    complement(1824..1833)
                     /label=ColE1 ORI
                     /note="ColE1 ORI"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    complement(2257..2482)
                     /label=ROP/ROM
                     /note="ROP/ROM"
                     /note="ApEinfo_revcolor=#ff9ccd
                     ApEinfo_fwdcolor=#ff9ccd"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    4147..5349
                     /label=MBP ORF
                     /note="MBP ORF"
                     /note="ApEinfo_revcolor=#84b0dc
                     ApEinfo_fwdcolor=#84b0dc"
     misc_feature    4159..4176
                     /label=His6 tag
                     /note="His6 tag"
                     /note="ApEinfo_revcolor=#5757ff
                     ApEinfo_fwdcolor=#5757ff"
     misc_feature    5325..6824
                     /label=WP_022785443.1-gB1
                     /note="WP_022785443.1-gB1"
                     /note="ApEinfo_revcolor=#c7b0e3
                     ApEinfo_fwdcolor=#c7b0e3"
     misc_feature    5350..5364
                     /label=Lic_v1_For
                     /note="Lic_v1_For"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    5368..9678
                     /label=WP_022785443.1
                     /note="WP_022785443.1"
                     /note="ApEinfo_revcolor=#f8d3a9
                     ApEinfo_fwdcolor=#f8d3a9"
     misc_feature    6785..8284
                     /label=WP_022785443.1-gB2
                     /note="WP_022785443.1-gB2"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    8245..9725
                     /label=WP_022785443.1-gB3
                     /note="WP_022785443.1-gB3"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    9686..9700
                     /label=Lic_v1_rev
                     /note="Lic_v1_rev"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    9807..9825
                     /label=T7 terminator primer
                     /note="T7 terminator primer"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    9828..9868
                     /label=T7 terminator
                     /note="T7 terminator"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgcaatg aagatcagta aagtacgcga agaaaaccgt
     5401 ggggccaaat tgactgtgaa cgccaagact gctgtagtgt ccgagaaccg ttcgcaagaa
     5461 gggatcctgt ataatgaccc aagtcgttac ggtaaaagcc gcaaaaatga cgaggatcgt
     5521 gaccgctaca tcgagtcccg tcttaagtct tctgggaaat tataccgcat ttttaatgag
     5581 gacaagaaca aacgcgagac agacgaactg caatggttcc ttagtgagat cgtgaagaag
     5641 atcaatcgtc gcaatggttt ggtattatcg gatatgctta gtgtggacga ccgcgcattc
     5701 gagaaagcct tcgaaaagta tgcagagtta tcgtatacga atcgccgtaa taaagtttct
     5761 ggtagcccgg cgttcgagac ctgcggagta gatgcagcaa cagctgaacg tttaaaaggc
     5821 attatctcgg aaaccaactt tatcaaccgt atcaaaaaca atatcgacaa caaagtctcg
     5881 gaggacatta tcgatcgtat tatcgctaag tacttgaaga aaagcttgtg tcgtgagcgt
     5941 gtcaaacgtg ggttaaagaa acttttaatg aatgccttcg acttgcctta cagtgaccct
     6001 gacatcgatg tgcaacgcga ttttattgat tacgttttag aggatttcta ccatgttcgt
     6061 gccaagtcac aagtgtctcg ttcgatcaaa aacatgaata tgccggtgca gcccgaaggc
     6121 gatggtaagt ttgcgatcac agtaagtaag ggtgggacgg aatcggggaa caaacgctct
     6181 gccgaaaaag aagcattcaa aaagttcctg agtgattacg cgtcgctgga tgaacgtgtt
     6241 cgtgacgaca tgcttcgtcg catgcgccgc ctggtcgtct tgtactttta cggatcagac
     6301 gatagcaaat tatcagatgt taacgaaaaa ttcgacgtat gggaggacca tgctgcccgc
     6361 cgcgtagaca atcgcgagtt catcaagctt ccgttggaga ataaacttgc caacggcaag
     6421 accgataaag atgcggaacg tatccgtaag aacaccgtta aagaactgta tcgtaaccaa
     6481 aatattggat gctatcgcca ggctgttaaa gccgtagagg aggacaacaa cggtcgctac
     6541 ttcgacgaca agatgctgaa catgttcttt attcaccgca tcgagtacgg tgtggaaaag
     6601 atttacgcca atttgaaaca agtcacggag ttcaaggccc gcactgggta tctttcagag
     6661 aaaatctgga aagacttgat caattacatt tccattaaat acatcgctat gggcaaggca
     6721 gtctacaatt acgccatgga tgagctgaat gcctcagaca aaaaggagat cgagctgggc
     6781 aagatcagtg aagaatattt gtccgggatc agttcgtttg actatgaatt gatcaaagcc
     6841 gaagagatgc tgcaacgcga gaccgctgta tatgttgctt tcgcggctcg ccacttgtcc
     6901 tcccagaccg ttgaactgga ttccgagaac tccgacttcc ttcttttgaa gccaaaggga
     6961 actatggaca agaacgataa aaacaagtta gcctccaaca acattcttaa ttttcttaag
     7021 gacaaggaaa cacttcgcga taccatttta cagtactttg gcggccacag tctgtggacc
     7081 gactttccgt ttgacaaata cttggccggc gggaaagacg acgtcgactt cctgacagac
     7141 cttaaagatg tgatttactc catgcgtaat gatagttttc actatgctac tgaaaaccac
     7201 aacaatggaa aatggaataa ggaattaatt agtgcaatgt ttgaacatga aacagagcgt
     7261 atgacagtag tcatgaaaga taaattctac tcgaataatc ttcctatgtt ctataagaat
     7321 gacgacttga agaaattgct gatcgatttg tacaaggata acgtagagcg tgcgagccag
     7381 gtcccatctt ttaacaaggt cttcgtgcgc aagaattttc ctgctttggt tcgcgataaa
     7441 gacaacttgg ggattgaatt agaccttaag gcggacgcgg ataaaggtga gaacgagtta
     7501 aaattctaca acgctcttta ttacatgttt aaagagatct actataacgc gttcttaaat
     7561 gataagaatg tgcgtgaacg ttttattact aaggcgacga aagtggccga taactatgat
     7621 cgcaataagg agcgcaacct taaagaccgt attaagtccg cggggtcgga tgagaaaaaa
     7681 aagttacgtg aacagctgca gaattacatt gctgagaatg acttcgggca gcgtatcaag
     7741 aatatcgtgc aggtcaatcc ggattatacc ctggctcaaa tctgccaact tattatgacg
     7801 gagtataatc aacaaaacaa cgggtgcatg cagaaaaagt ctgctgcccg caaagacatc
     7861 aataaggata gttaccagca ttacaagatg ctgcttttgg ttaatttacg caaagcattt
     7921 ttggaattta ttaaggagaa ttatgctttt gttttgaaac cctataaaca cgacctttgt
     7981 gataaagccg atttcgtgcc agatttcgcc aaatacgtca aaccctacgc tggcttgatc
     8041 agccgtgtag caggatcaag tgagctgcaa aagtggtaca tcgtgagccg cttcttgtct
     8101 ccggcacagg ccaatcacat gcttgggttc ctgcactcgt acaagcagta cgtatgggat
     8161 atttaccgcc gcgcatccga aaccggtaca gagattaacc actctatcgc agaggacaag
     8221 attgctgggg ttgacattac cgacgtcgac gcagtgatcg atttatcggt gaaattgtgc
     8281 ggaacaatct ctagcgagat ttcggattat ttcaaggatg acgaggtcta cgcggagtac
     8341 atctcatcat atttagattt cgaatacgat ggtggtaatt acaaagactc gctgaaccgt
     8401 ttctgtaatt cagacgcagt aaatgaccaa aaagtggcac tgtattatga cggcgaacac
     8461 ccgaaattga atcgtaacat tatcttgtcg aaactttacg gagagcgccg tttcttggag
     8521 aaaatcactg accgcgtttc tcgttcagat attgtggagt attacaagct gaagaaggag
     8581 acgtcgcagt atcagacgaa agggattttc gatagtgagg acgaacagaa gaatattaag
     8641 aagtttcagg agatgaaaaa catcgtggaa tttcgtgatc tgatggatta ctcggagatc
     8701 gcggacgaat tacaaggaca actgatcaac tggatttatc ttcgtgaacg tgacctgatg
     8761 aacttccaac tggggtatca ttacgcctgc ttgaacaacg actcaaataa gcaagctact
     8821 tatgtaactc ttgattacca aggtaaaaaa aaccgcaaga ttaacggtgc gattttatat
     8881 cagatttgtg caatgtatat taatggactg ccgctttact acgtcgataa agacagttcg
     8941 gagtggacgg tgagcgacgg taaggaatcc acgggagcca agattggtga attctaccgc
     9001 tatgcaaagt cctttgaaaa tacgtctgac tgctacgctt cgggcttaga gatcttcgag
     9061 aatatcagtg agcacgacaa catcacggaa ttacgcaact acattgaaca ttttcgttac
     9121 tacagctctt ttgaccgttc ctttttgggg atctattcag aggtgtttga tcgctttttc
     9181 acatacgact taaagtatcg caaaaacgta cctaccatct tgtataatat tttactgcaa
     9241 cactttgtta atgtccgctt cgagttcgta agcggcaaaa aaatgattgg cattgacaaa
     9301 aaggaccgca agattgccaa agagaaggaa tgcgctcgta ttacaatccg cgaaaagaac
     9361 ggtgtctact cggagcagtt cacttacaag ttgaagaatg ggaccgtgta tgtcgatgcg
     9421 cgtgacaaac gctacctgca gtccattatt cgtcttttgt tttaccccga aaaggtcaac
     9481 atggacgaaa tgatcgaagt taaggaaaag aagaagccga gtgacaataa tacgggaaag
     9541 ggctatagca aacgcgaccg ccagcaggat cgtaaagaat atgacaagta caaagaaaaa
     9601 aagaaaaagg aggggaattt cctttcaggt atgggaggca atatcaactg ggacgagatt
     9661 aacgctcaac ttaagaacta ataacattgg aagtggataa cggatccgcg atcgcggcgc
     9721 gccacctggt ggccggccgg taccacgcgt gcgcgctgat ccggctgcta acaaagcccg
     9781 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     9841 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca
     9901 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     9961 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
    10021 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
    10081 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
    10141 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
    10201 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
    10261 aaacatgaga a
//
