LOCUS       Exported                9815 bp ds-DNA     circular SYN 13-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p2CT-His-MBP-Rca_Cas13a_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 13, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(139..167)
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    209..1069
                     /label=AMP resistance beta-lactimase
                     /note="AMP resistance beta-lactimase"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     CDS             407..1066
                     /label=AmpR
                     /note="AmpR"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     rep_origin      1164..1846
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="ApEinfo_revcolor=#f8d3a9
                     ApEinfo_fwdcolor=#f8d3a9"
     misc_feature    complement(1276..1385)
                     /label=RNA1-for
                     /note="RNA1-for"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    complement(1824..1833)
                     /label=ColE1 ORI
                     /note="ColE1 ORI"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    complement(2257..2482)
                     /label=ROP/ROM
                     /note="ROP/ROM"
                     /note="ApEinfo_revcolor=#ff9ccd
                     ApEinfo_fwdcolor=#ff9ccd"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    4147..5349
                     /label=MBP ORF
                     /note="MBP ORF"
                     /note="ApEinfo_revcolor=#84b0dc
                     ApEinfo_fwdcolor=#84b0dc"
     misc_feature    4159..4176
                     /label=His6 tag
                     /note="His6 tag"
                     /note="ApEinfo_revcolor=#5757ff
                     ApEinfo_fwdcolor=#5757ff"
     misc_feature    5325..7324
                     /label=ETD7_gB1
                     /note="ETD7_gB1"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     misc_feature    5350..5364
                     /label=Lic_v1_For
                     /note="Lic_v1_For"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    5368..9222
                     /label=ETD76934.1
                     /note="ETD76934.1"
                     /note="ApEinfo_revcolor=#b7e6d7
                     ApEinfo_fwdcolor=#b7e6d7"
     misc_feature    7285..9269
                     /label=ETD7_gB2
                     /note="ETD7_gB2"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    9230..9244
                     /label=Lic_v1_rev
                     /note="Lic_v1_rev"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    9351..9369
                     /label=T7 terminator primer
                     /note="T7 terminator primer"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    9372..9412
                     /label=T7 terminator
                     /note="T7 terminator"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgcaatg cagattggaa aagttcaagg ccgcacgatt
     5401 tccgagttcg gcgaccctgc tggcggctta aaacgtaaaa tttcgacaga cggtaagaat
     5461 cgcaaagagt tgcctgcaca tcttagctca gatccgaagg ctctgatcgg tcagtggatc
     5521 agtgggatcg ataaaatcta tcgcaagcca gattctcgta agtcggacgg caaggctatt
     5581 cacagcccca ctccgtcgaa gatgcagttt gatgcacgtg acgacttagg tgaagccttc
     5641 tggaaacttg ttagcgaagc cgggttggcc caagattcgg attacgacca atttaaacgt
     5701 cgtcttcatc catatgggga caagttccaa cctgcagact cgggagctaa attgaagttc
     5761 gaggcggacc caccagaacc gcaagcgttt cacggccgct ggtacggtgc tatgagcaaa
     5821 cgcggaaatg acgcgaaaga gctggcagct gcattatacg agcatcttca tgtcgacgaa
     5881 aagcgcatcg acggtcagcc aaaacgcaac cccaagacgg ataaatttgc gcccggatta
     5941 gtcgtggctc gcgctctggg tattgagtcc tcggttttac cgcgtgggat ggcgcgtctt
     6001 gcacgtaatt ggggcgagga ggaaattcaa acttatttcg tcgtcgacgt cgcagctagc
     6061 gtaaaagaag ttgcgaaggc ggccgtatcg gccgctcagg ctttcgaccc tccacgtcaa
     6121 gtttcgggcc gttcgctttc acccaaggta ggttttgcct tagctgagca tctggaacgc
     6181 gtcactggta gcaagcgttg tagctttgac cctgctgcag gccccagtgt attggcgctg
     6241 catgatgagg taaagaaaac ttacaagcgt ttgtgcgcgc gtggtaagaa tgctgcgcgt
     6301 gcgtttcccg ctgacaaaac ggaattgtta gcattgatgc gccataccca tgaaaaccgc
     6361 gtgcgtaacc agatggtgcg tatgggacgc gtcagcgagt accgtgggca acaagccggt
     6421 gacctggcgc agtcacatta ttggacgagc gcaggacaaa ctgaaattaa ggagtctgaa
     6481 atctttgtcc gtctttgggt aggtgccttt gccttagctg ggcgttccat gaaagcatgg
     6541 attgacccca tgggaaagat tgtaaacaca gaaaagaatg accgtgattt gactgccgct
     6601 gtgaatatcc gccaagtgat ctccaataaa gaaatggtgg cagaagctat ggctcgtcgc
     6661 ggtatttact ttggcgagac tccggaattg gatcgcttgg gggcggaagg caatgaagga
     6721 tttgtttttg cgttattacg ttatttacgt gggtgtcgta accagacctt ccatctgggc
     6781 gcacgtgctg gttttttaaa agaaatccgc aaggagcttg aaaagacacg ttgggggaag
     6841 gcaaaagagg cggagcacgt tgtgttaaca gataagacgg tagctgcaat ccgtgcgatt
     6901 attgacaatg atgcgaaggc tttaggagca cgcctgctgg ccgacttatc cggagcattt
     6961 gtagcgcact acgcatctaa agagcatttt tcgaccttgt actctgagat cgttaaggct
     7021 gtcaaggacg ctcccgaagt ctcgtcagga ttgccccgct tgaaactgct gcttaagcgc
     7081 gccgacgggg tgcgcgggta tgttcatggg ttgcgtgaca ctcgtaagca tgctttcgcc
     7141 accaaattgc ccccaccacc ggcgccacgt gagcttgacg acccagctac caaagcacgt
     7201 tatattgctt tgctgcgcct gtatgacggt ccatttcgcg cctatgcgtc aggcatcaca
     7261 gggacggctt tggccggacc cgcggcgcgt gctaaggaag ctgccactgc ccttgcacaa
     7321 agtgtcaacg ttaccaaagc atattcagat gttatggagg ggcgctcttc acgcctgcgc
     7381 ccgcccaacg acggtgagac cctgcgtgag tacttaagtg ctttaacggg cgagactgcg
     7441 accgaattcc gtgtccaaat cggatacgag tcggattctg aaaacgcacg caaacaagcg
     7501 gaatttattg agaattatcg ccgtgatatg ttggctttta tgtttgagga ttacattcgc
     7561 gctaaaggtt ttgattggat cttaaaaatc gaaccgggag ccacagcaat gacccgtgca
     7621 ccagtgctgc ctgaacctat tgacacacgt ggacaatacg aacattggca ggcggcattg
     7681 tatctggtta tgcattttgt gcccgcgtcc gacgtaagta atcttcttca tcagttacgt
     7741 aaatgggagg ctcttcaagg taaatatgaa ttagtacagg atggcgacgc aaccgaccaa
     7801 gcggatgcac gtcgtgaggc tttggattta gttaaacgtt tccgcgacgt tttggtactt
     7861 tttcttaaga caggagaggc acgcttcgaa ggccgcgcgg ctccctttga tttgaagcct
     7921 ttccgtgctt tgttcgcgaa ccccgcgact tttgaccgcc tgttcatggc cacacctaca
     7981 accgcacgtc cggcggaaga tgaccccgaa ggtgacgggg caagcgaacc cgaacttcgc
     8041 gtcgcacgca cgctgcgcgg acttcgccag atcgcgcgct acaaccacat ggcagttttg
     8101 tcggatcttt tcgcgaaaca taaggtgcgc gatgaagaag tggcccgctt agccgaaatt
     8161 gaggatgaga cgcaggaaaa atcccagatt gttgcggcac aggaactgcg tacggatttg
     8221 catgacaagg taatgaagtg ccatccaaag acgattagtc ctgaagaacg tcagagttac
     8281 gcggccgcca tcaagaccat cgaggagcat cgttttctgg ttgggcgtgt gtacttggga
     8341 gatcatttac gtttgcatcg tctgatgatg gatgtgatcg gacgtttgat cgactatgca
     8401 ggcgcctatg aacgtgatac cggcacgttt cttattaatg cttcaaagca attgggtgct
     8461 ggagccgact gggctgtcac gatcgctggt gcggcgaaca cagacgctcg tacacaaacg
     8521 cgtaaggacc ttgcgcactt taatgtgttg gaccgtgcag atggcactcc cgatttaacc
     8581 gcgttggtga accgcgctcg tgagatgatg gcttacgacc gtaaacgcaa gaatgctgta
     8641 cctcgctcaa ttttagatat gctggctcgt ctgggattga ctttaaaatg gcaaatgaaa
     8701 gatcatttgt tgcaagacgc tactattacc caggcggcca tcaagcactt agacaaggtg
     8761 cgcttgaccg tcggaggccc tgccgcagta actgaagccc gtttcagcca agactacttg
     8821 cagatggtag ctgctgtatt taatggaagt gtacagaatc ctaagcctcg ccgtcgcgac
     8881 gatggtgacg cctggcacaa gccacccaag ccagccacgg cgcaaagtca acctgaccaa
     8941 aagccaccca acaaagcgcc ttccgctggt tctcgtcttc cgcctcccca agttggtgag
     9001 gtgtacgaag gggtagttgt gaaggtcatt gacacaggct cacttgggtt ccttgccgtg
     9061 gagggggtcg caggcaatat cggtcttcac atctcacgtc ttcgtcgtat tcgtgaagac
     9121 gcgattattg taggacgtcg ttatcgcttt cgcgtagaga tttatgtgcc tcctaagtcg
     9181 aatactagca agttaaacgc tgcggacctg gttcgtatcg attaataaca ttggaagtgg
     9241 ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc
     9301 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     9361 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     9421 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     9481 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     9541 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     9601 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     9661 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     9721 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     9781 aaagcttatc gatgataagc tgtcaaacat gagaa
//
