LOCUS Exported 9815 bp ds-DNA circular SYN 13-APR-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS p2CT-His-MBP-Hhe_Cas13a_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9815) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Apr 13, 2017 from SnapGene 3.3.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9815 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature complement(139..167) /label=AmpR_promoter /note="AmpR_promoter" /note="ApEinfo_revcolor=#b1ff67 ApEinfo_fwdcolor=#b1ff67" misc_feature 209..1069 /label=AMP resistance beta-lactimase /note="AMP resistance beta-lactimase" /note="ApEinfo_revcolor=#ffef86 ApEinfo_fwdcolor=#ffef86" CDS 407..1066 /label=AmpR /note="AmpR" /note="ApEinfo_revcolor=#ffef86 ApEinfo_fwdcolor=#ffef86" rep_origin 1164..1846 /label=ColE1 origin /note="ColE1 origin" /note="ApEinfo_revcolor=#f8d3a9 ApEinfo_fwdcolor=#f8d3a9" misc_feature complement(1276..1385) /label=RNA1-for /note="RNA1-for" /note="ApEinfo_revcolor=#85dae9 ApEinfo_fwdcolor=#85dae9" misc_feature complement(1824..1833) /label=ColE1 ORI /note="ColE1 ORI" /note="ApEinfo_revcolor=#b1ff67 ApEinfo_fwdcolor=#b1ff67" misc_feature complement(2257..2482) /label=ROP/ROM /note="ROP/ROM" /note="ApEinfo_revcolor=#ff9ccd ApEinfo_fwdcolor=#ff9ccd" primer_bind 4010..4029 /label=T7 /note="T7" /note="ApEinfo_revcolor=#85dae9 ApEinfo_fwdcolor=#85dae9" misc_feature 4147..5349 /label=MBP ORF /note="MBP ORF" /note="ApEinfo_revcolor=#84b0dc ApEinfo_fwdcolor=#84b0dc" misc_feature 4159..4176 /label=His6 tag /note="His6 tag" /note="ApEinfo_revcolor=#5757ff ApEinfo_fwdcolor=#5757ff" misc_feature 5325..7324 /label=gB1_CVTD020000026.1 /note="gB1_CVTD020000026.1" /note="ApEinfo_revcolor=#d59687 ApEinfo_fwdcolor=#d59687" misc_feature 5350..5364 /label=Lic_v1_For /note="Lic_v1_For" /note="ApEinfo_revcolor=#b1ff67 ApEinfo_fwdcolor=#b1ff67" misc_feature 5368..9222 /label=CVTD020000026.1 /note="CVTD020000026.1" /note="ApEinfo_revcolor=#d6b295 ApEinfo_fwdcolor=#d6b295" misc_feature 7285..9269 /label=gB2_CVTD020000026.1 /note="gB2_CVTD020000026.1" /note="ApEinfo_revcolor=#75c6a9 ApEinfo_fwdcolor=#75c6a9" misc_feature 9230..9244 /label=Lic_v1_rev /note="Lic_v1_rev" /note="ApEinfo_revcolor=#faac61 ApEinfo_fwdcolor=#faac61" misc_feature 9351..9369 /label=T7 terminator primer /note="T7 terminator primer" /note="ApEinfo_revcolor=#85dae9 ApEinfo_fwdcolor=#85dae9" misc_feature 9372..9412 /label=T7 terminator /note="T7 terminator" /note="ApEinfo_revcolor=#ffef86 ApEinfo_fwdcolor=#ffef86" ORIGIN 1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact 781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc 2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt 3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc 3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc 3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc 3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc 3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc 3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc 4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa 4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat 4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa 4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt 4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa 4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac 4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg 4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt 4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac 4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag 4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac 4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat 4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg 4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg 4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta 4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca 4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg 5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg 5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc 5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt 5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg 5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag 5341 gaaaacctgt acttccaatc caatgcaatg aagttgacgc gtcgccgcat cagtggcaat 5401 tcagtcgacc agaagattac agctgcgttt tatcgtgata tgtcgcaagg gttattatat 5461 tatgattcgg aggacaatga ctgtacggac aaggtcatcg aaagtatgga tttcgaacgc 5521 tcttggcgtg gacgcattct gaagaacggc gaggatgata agaatccgtt ttacatgttc 5581 gttaaaggtt tagttggttc caacgataag atcgtgtgcg aacctatcga cgtcgactca 5641 gaccctgata acttagatat cttgattaac aaaaatctga cggggttcgg acgcaacttg 5701 aaagcacctg acagtaacga taccttagaa aatcttatcc gcaaaatcca ggccggcatt 5761 cccgaagaag aggttcttcc ggagctgaag aaaattaaag agatgatcca gaaagacatc 5821 gttaaccgca aagagcagtt gcttaaatcc atcaaaaaca accgtattcc tttttcattg 5881 gaaggttcca agctggtccc ttccaccaaa aagatgaagt ggcttttcaa gttgattgac 5941 gttcctaata aaaccttcaa tgaaaaaatg ttggagaagt actgggagat ctacgactat 6001 gacaagctta aggcgaatat cacgaaccgc ctggacaaaa cggataagaa ggcacgttca 6061 atttcgcgtg cggtaagcga ggagttacgt gagtaccata aaaacttgcg cacaaactat 6121 aatcgcttcg tatctggaga ccgtccagca gccgggcttg acaatggggg gagtgctaaa 6181 tataatcccg ataaggagga gttcttattg tttcttaaag aagttgagca gtacttcaag 6241 aaatacttcc cagtcaagag caagcattcg aacaagtcca aagacaaatc attggttgat 6301 aagtacaaaa attattgcag ttacaaagtg gtcaagaaag aggtcaaccg cagcatcatc 6361 aatcaattgg tggctggact tattcagcag ggtaagcttc tgtattactt ctactataac 6421 gatacctggc aggaggattt cctgaattcc tacggtctta gttatattca ggttgaggag 6481 gcatttaaaa agagtgtaat gaccagtctt tcgtggggaa tcaaccgtct gacctcgttc 6541 tttatcgacg actcgaacac tgtgaagttt gatgatatca caacgaaaaa ggctaaggag 6601 gcgattgagt ctaattattt caacaaatta cgcacatgct cccgtatgca ggaccacttc 6661 aaagagaaat tggccttctt ttacccggtg tatgtgaagg acaagaagga tcgcccggat 6721 gatgatatcg aaaacttaat tgtactggtt aagaacgcca ttgagagcgt gagctatttg 6781 cgcaatcgca cgtttcactt taaagagagc tccttattgg agctgcttaa agagttagac 6841 gataaaaatt ctggtcaaaa taaaatcgac tatagcgtag ccgctgaatt tattaaacgc 6901 gatatcgaga acttgtatga cgtcttccgt gaacagatcc gctcacttgg tattgctgag 6961 tactacaagg cggacatgat ttcggactgt tttaagacat gcgggttaga attcgcgctt 7021 tattcgccaa aaaattcgtt gatgcccgct tttaagaacg tttataaacg cggagcaaat 7081 cttaacaagg cgtatattcg cgataaaggg ccaaaggaaa caggagacca gggtcagaac 7141 tcgtacaaag ctttggagga atatcgtgag cttacatggt atattgaggt gaagaataac 7201 gaccagtcgt acaacgccta caagaatttg cttcaactga tttattatca tgcgtttctt 7261 ccggaggttc gtgaaaatga ggctcttatc actgacttca ttaaccgcac taaagagtgg 7321 aatcgtaagg aaacagagga gcgtctgaac actaaaaaca ataaaaaaca caagaatttc 7381 gacgagaatg acgatattac ggtaaatacg tatcgttacg aatctattcc ggattaccag 7441 ggggaatccc ttgatgatta tcttaaggtg ttacagcgca agcaaatggc gcgtgcgaag 7501 gaggtaaatg agaaggagga gggcaacaat aactatatcc aattcattcg tgacgtcgta 7561 gtatgggcgt tcggggccta cttggaaaac aagctgaaga attataagaa cgaactgcaa 7621 ccaccgttga gcaaagagaa cattggctta aatgatacct taaaggaatt gtttcccgaa 7681 gaaaaagtaa agtcaccatt taatatcaag tgccgtttct ccattagtac atttatcgac 7741 aacaaaggta agtccaccga taatacctcg gccgaggccg tcaagactga tggcaaggag 7801 gacgaaaagg acaaaaagaa tattaaacgc aaagacttgc tttgctttta cctgttctta 7861 cgcttgcttg atgaaaacga aatttgcaaa ttacaacacc aatttattaa ataccgttgc 7921 agtttaaaag agcgtcgttt tccaggtaac cgcaccaaac tggagaagga aactgaactt 7981 ttggccgagc tggaagaact gatggaactg gtacgtttta cgatgccatc aattcctgaa 8041 atctctgcaa aggcggaatc aggttatgac acaatgatta agaagtattt taaagacttc 8101 atcgagaaaa aagtctttaa gaatccgaag acgtccaacc tgtactacca ctccgactct 8161 aaaactccgg tcacccgcaa atatatggct ctgctgatgc gttctgcgcc tttgcatctc 8221 tacaaggata tcttcaaggg ctactatttg atcactaaga aggagtgttt ggagtatatt 8281 aaattaagca acatcattaa agactatcaa aactcattga atgaattgca cgagcagctg 8341 gagcgcatta aattaaagag tgagaaacaa aacgggaaag actctctgta tcttgacaaa 8401 aaggacttct ataaagtaaa ggagtacgtc gaaaatttag aacaagtggc gcgttacaag 8461 cacttgcagc acaaaatcaa cttcgaaagc ttataccgta tttttcgtat tcacgtggac 8521 atcgctgcgc gtatggtcgg ttatacccaa gactgggagc gtgatatgca ctttcttttc 8581 aaggcgttgg tctataatgg cgtacttgag gagcgccgtt ttgaagcaat ctttaacaat 8641 aacgatgaca acaacgacgg gcgtatcgta aaaaagatcc agaataactt aaataacaag 8701 aaccgcgaat tagtatctat gttatgttgg aataaaaagc tgaacaagaa cgaatttggt 8761 gctattattt ggaagcgtaa ccctatcgcc catctgaacc actttacaca aacggaacaa 8821 aactcaaagt cgagccttga atctctgatc aactcgctgc gcatcctgtt agcgtacgac 8881 cgtaaacgtc aaaacgctgt gacaaagact atcaatgacc ttctgttaaa cgactatcac 8941 atccgcatta agtgggaagg gcgcgtggat gaagggcaga tctactttaa tatcaaggaa 9001 aaggaagaca tcgagaacga gcctattatc cacttgaaac acctgcataa aaaggactgc 9061 tacatttaca agaacagcta tatgttcgac aagcaaaagg agtggatttg taatggcatt 9121 aaagaagaag tttatgacaa gtccattctt aagtgtatcg gaaacttgtt caagtttgac 9181 tatgaggaca aaaacaaatc ctcagcaaat cctaagcaca cctaataaca ttggaagtgg 9241 ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc 9301 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca 9361 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg 9421 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag 9481 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc 9541 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt 9601 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa 9661 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt 9721 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt 9781 aaagcttatc gatgataagc tgtcaaacat gagaa //