LOCUS       Exported                9815 bp ds-DNA     circular SYN 13-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p2CT-His-MBP-Hhe_Cas13a_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 13, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(139..167)
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    209..1069
                     /label=AMP resistance beta-lactimase
                     /note="AMP resistance beta-lactimase"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     CDS             407..1066
                     /label=AmpR
                     /note="AmpR"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     rep_origin      1164..1846
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="ApEinfo_revcolor=#f8d3a9
                     ApEinfo_fwdcolor=#f8d3a9"
     misc_feature    complement(1276..1385)
                     /label=RNA1-for
                     /note="RNA1-for"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    complement(1824..1833)
                     /label=ColE1 ORI
                     /note="ColE1 ORI"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    complement(2257..2482)
                     /label=ROP/ROM
                     /note="ROP/ROM"
                     /note="ApEinfo_revcolor=#ff9ccd
                     ApEinfo_fwdcolor=#ff9ccd"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    4147..5349
                     /label=MBP ORF
                     /note="MBP ORF"
                     /note="ApEinfo_revcolor=#84b0dc
                     ApEinfo_fwdcolor=#84b0dc"
     misc_feature    4159..4176
                     /label=His6 tag
                     /note="His6 tag"
                     /note="ApEinfo_revcolor=#5757ff
                     ApEinfo_fwdcolor=#5757ff"
     misc_feature    5325..7324
                     /label=gB1_CVTD020000026.1
                     /note="gB1_CVTD020000026.1"
                     /note="ApEinfo_revcolor=#d59687
                     ApEinfo_fwdcolor=#d59687"
     misc_feature    5350..5364
                     /label=Lic_v1_For
                     /note="Lic_v1_For"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    5368..9222
                     /label=CVTD020000026.1
                     /note="CVTD020000026.1"
                     /note="ApEinfo_revcolor=#d6b295
                     ApEinfo_fwdcolor=#d6b295"
     misc_feature    7285..9269
                     /label=gB2_CVTD020000026.1
                     /note="gB2_CVTD020000026.1"
                     /note="ApEinfo_revcolor=#75c6a9
                     ApEinfo_fwdcolor=#75c6a9"
     misc_feature    9230..9244
                     /label=Lic_v1_rev
                     /note="Lic_v1_rev"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    9351..9369
                     /label=T7 terminator primer
                     /note="T7 terminator primer"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    9372..9412
                     /label=T7 terminator
                     /note="T7 terminator"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgcaatg aagttgacgc gtcgccgcat cagtggcaat
     5401 tcagtcgacc agaagattac agctgcgttt tatcgtgata tgtcgcaagg gttattatat
     5461 tatgattcgg aggacaatga ctgtacggac aaggtcatcg aaagtatgga tttcgaacgc
     5521 tcttggcgtg gacgcattct gaagaacggc gaggatgata agaatccgtt ttacatgttc
     5581 gttaaaggtt tagttggttc caacgataag atcgtgtgcg aacctatcga cgtcgactca
     5641 gaccctgata acttagatat cttgattaac aaaaatctga cggggttcgg acgcaacttg
     5701 aaagcacctg acagtaacga taccttagaa aatcttatcc gcaaaatcca ggccggcatt
     5761 cccgaagaag aggttcttcc ggagctgaag aaaattaaag agatgatcca gaaagacatc
     5821 gttaaccgca aagagcagtt gcttaaatcc atcaaaaaca accgtattcc tttttcattg
     5881 gaaggttcca agctggtccc ttccaccaaa aagatgaagt ggcttttcaa gttgattgac
     5941 gttcctaata aaaccttcaa tgaaaaaatg ttggagaagt actgggagat ctacgactat
     6001 gacaagctta aggcgaatat cacgaaccgc ctggacaaaa cggataagaa ggcacgttca
     6061 atttcgcgtg cggtaagcga ggagttacgt gagtaccata aaaacttgcg cacaaactat
     6121 aatcgcttcg tatctggaga ccgtccagca gccgggcttg acaatggggg gagtgctaaa
     6181 tataatcccg ataaggagga gttcttattg tttcttaaag aagttgagca gtacttcaag
     6241 aaatacttcc cagtcaagag caagcattcg aacaagtcca aagacaaatc attggttgat
     6301 aagtacaaaa attattgcag ttacaaagtg gtcaagaaag aggtcaaccg cagcatcatc
     6361 aatcaattgg tggctggact tattcagcag ggtaagcttc tgtattactt ctactataac
     6421 gatacctggc aggaggattt cctgaattcc tacggtctta gttatattca ggttgaggag
     6481 gcatttaaaa agagtgtaat gaccagtctt tcgtggggaa tcaaccgtct gacctcgttc
     6541 tttatcgacg actcgaacac tgtgaagttt gatgatatca caacgaaaaa ggctaaggag
     6601 gcgattgagt ctaattattt caacaaatta cgcacatgct cccgtatgca ggaccacttc
     6661 aaagagaaat tggccttctt ttacccggtg tatgtgaagg acaagaagga tcgcccggat
     6721 gatgatatcg aaaacttaat tgtactggtt aagaacgcca ttgagagcgt gagctatttg
     6781 cgcaatcgca cgtttcactt taaagagagc tccttattgg agctgcttaa agagttagac
     6841 gataaaaatt ctggtcaaaa taaaatcgac tatagcgtag ccgctgaatt tattaaacgc
     6901 gatatcgaga acttgtatga cgtcttccgt gaacagatcc gctcacttgg tattgctgag
     6961 tactacaagg cggacatgat ttcggactgt tttaagacat gcgggttaga attcgcgctt
     7021 tattcgccaa aaaattcgtt gatgcccgct tttaagaacg tttataaacg cggagcaaat
     7081 cttaacaagg cgtatattcg cgataaaggg ccaaaggaaa caggagacca gggtcagaac
     7141 tcgtacaaag ctttggagga atatcgtgag cttacatggt atattgaggt gaagaataac
     7201 gaccagtcgt acaacgccta caagaatttg cttcaactga tttattatca tgcgtttctt
     7261 ccggaggttc gtgaaaatga ggctcttatc actgacttca ttaaccgcac taaagagtgg
     7321 aatcgtaagg aaacagagga gcgtctgaac actaaaaaca ataaaaaaca caagaatttc
     7381 gacgagaatg acgatattac ggtaaatacg tatcgttacg aatctattcc ggattaccag
     7441 ggggaatccc ttgatgatta tcttaaggtg ttacagcgca agcaaatggc gcgtgcgaag
     7501 gaggtaaatg agaaggagga gggcaacaat aactatatcc aattcattcg tgacgtcgta
     7561 gtatgggcgt tcggggccta cttggaaaac aagctgaaga attataagaa cgaactgcaa
     7621 ccaccgttga gcaaagagaa cattggctta aatgatacct taaaggaatt gtttcccgaa
     7681 gaaaaagtaa agtcaccatt taatatcaag tgccgtttct ccattagtac atttatcgac
     7741 aacaaaggta agtccaccga taatacctcg gccgaggccg tcaagactga tggcaaggag
     7801 gacgaaaagg acaaaaagaa tattaaacgc aaagacttgc tttgctttta cctgttctta
     7861 cgcttgcttg atgaaaacga aatttgcaaa ttacaacacc aatttattaa ataccgttgc
     7921 agtttaaaag agcgtcgttt tccaggtaac cgcaccaaac tggagaagga aactgaactt
     7981 ttggccgagc tggaagaact gatggaactg gtacgtttta cgatgccatc aattcctgaa
     8041 atctctgcaa aggcggaatc aggttatgac acaatgatta agaagtattt taaagacttc
     8101 atcgagaaaa aagtctttaa gaatccgaag acgtccaacc tgtactacca ctccgactct
     8161 aaaactccgg tcacccgcaa atatatggct ctgctgatgc gttctgcgcc tttgcatctc
     8221 tacaaggata tcttcaaggg ctactatttg atcactaaga aggagtgttt ggagtatatt
     8281 aaattaagca acatcattaa agactatcaa aactcattga atgaattgca cgagcagctg
     8341 gagcgcatta aattaaagag tgagaaacaa aacgggaaag actctctgta tcttgacaaa
     8401 aaggacttct ataaagtaaa ggagtacgtc gaaaatttag aacaagtggc gcgttacaag
     8461 cacttgcagc acaaaatcaa cttcgaaagc ttataccgta tttttcgtat tcacgtggac
     8521 atcgctgcgc gtatggtcgg ttatacccaa gactgggagc gtgatatgca ctttcttttc
     8581 aaggcgttgg tctataatgg cgtacttgag gagcgccgtt ttgaagcaat ctttaacaat
     8641 aacgatgaca acaacgacgg gcgtatcgta aaaaagatcc agaataactt aaataacaag
     8701 aaccgcgaat tagtatctat gttatgttgg aataaaaagc tgaacaagaa cgaatttggt
     8761 gctattattt ggaagcgtaa ccctatcgcc catctgaacc actttacaca aacggaacaa
     8821 aactcaaagt cgagccttga atctctgatc aactcgctgc gcatcctgtt agcgtacgac
     8881 cgtaaacgtc aaaacgctgt gacaaagact atcaatgacc ttctgttaaa cgactatcac
     8941 atccgcatta agtgggaagg gcgcgtggat gaagggcaga tctactttaa tatcaaggaa
     9001 aaggaagaca tcgagaacga gcctattatc cacttgaaac acctgcataa aaaggactgc
     9061 tacatttaca agaacagcta tatgttcgac aagcaaaagg agtggatttg taatggcatt
     9121 aaagaagaag tttatgacaa gtccattctt aagtgtatcg gaaacttgtt caagtttgac
     9181 tatgaggaca aaaacaaatc ctcagcaaat cctaagcaca cctaataaca ttggaagtgg
     9241 ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc
     9301 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     9361 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     9421 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     9481 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     9541 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     9601 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     9661 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     9721 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     9781 aaagcttatc gatgataagc tgtcaaacat gagaa
//
