LOCUS       Exported                9422 bp ds-DNA     circular SYN 13-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p2CT-His-MBP-Ppr_Cas13a_WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Apr 13, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(139..167)
                     /label=AmpR_promoter
                     /note="AmpR_promoter"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    209..1069
                     /label=AMP resistance beta-lactimase
                     /note="AMP resistance beta-lactimase"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     CDS             407..1066
                     /label=AmpR
                     /note="AmpR"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
     rep_origin      1164..1846
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="ApEinfo_revcolor=#f8d3a9
                     ApEinfo_fwdcolor=#f8d3a9"
     misc_feature    complement(1276..1385)
                     /label=RNA1-for
                     /note="RNA1-for"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    complement(1824..1833)
                     /label=ColE1 ORI
                     /note="ColE1 ORI"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    complement(2257..2482)
                     /label=ROP/ROM
                     /note="ROP/ROM"
                     /note="ApEinfo_revcolor=#ff9ccd
                     ApEinfo_fwdcolor=#ff9ccd"
     primer_bind     4010..4029
                     /label=T7
                     /note="T7"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    4147..5349
                     /label=MBP ORF
                     /note="MBP ORF"
                     /note="ApEinfo_revcolor=#84b0dc
                     ApEinfo_fwdcolor=#84b0dc"
     misc_feature    4159..4176
                     /label=His6 tag
                     /note="His6 tag"
                     /note="ApEinfo_revcolor=#5757ff
                     ApEinfo_fwdcolor=#5757ff"
     misc_feature    5325..6524
                     /label=WP_013443710.1-gB1
                     /note="WP_013443710.1-gB1"
                     /note="ApEinfo_revcolor=#75c6a9
                     ApEinfo_fwdcolor=#75c6a9"
     misc_feature    5350..5364
                     /label=Lic_v1_For
                     /note="Lic_v1_For"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    5368..8829
                     /label=WP_013443710.1
                     /note="WP_013443710.1"
                     /note="ApEinfo_revcolor=#f58a5e
                     ApEinfo_fwdcolor=#f58a5e"
     misc_feature    6485..7734
                     /label=WP_013443710.1-gB2
                     /note="WP_013443710.1-gB2"
                     /note="ApEinfo_revcolor=#b7e6d7
                     ApEinfo_fwdcolor=#b7e6d7"
     misc_feature    7695..8876
                     /label=WP_013443710.1-gB3
                     /note="WP_013443710.1-gB3"
                     /note="ApEinfo_revcolor=#b1ff67
                     ApEinfo_fwdcolor=#b1ff67"
     misc_feature    8837..8851
                     /label=Lic_v1_rev
                     /note="Lic_v1_rev"
                     /note="ApEinfo_revcolor=#faac61
                     ApEinfo_fwdcolor=#faac61"
     misc_feature    8958..8976
                     /label=T7 terminator primer
                     /note="T7 terminator primer"
                     /note="ApEinfo_revcolor=#85dae9
                     ApEinfo_fwdcolor=#85dae9"
     misc_feature    8979..9019
                     /label=T7 terminator
                     /note="T7 terminator"
                     /note="ApEinfo_revcolor=#ffef86
                     ApEinfo_fwdcolor=#ffef86"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
     4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
     4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
     4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
     4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
     4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
     4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
     4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
     4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
     4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
     4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
     4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
     4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
     4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
     4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
     4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
     4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
     5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     5341 gaaaacctgt acttccaatc caatgcaatg cgtgtgtcga aagtgaaagt taaagatggg
     5401 ggcaaagaca agatggtctt ggtccatcgt aagacgaccg gtgcccaact tgtttattca
     5461 gggcagcccg ttagcaacga gacgtctaat attctgccag aaaaaaagcg ccagtcattc
     5521 gaccttagca ccctgaacaa aacaattatc aaattcgata ctgcgaaaaa gcaaaagtta
     5581 aatgtggacc agtataagat cgtagaaaag atcttcaagt atcccaagca ggagctgccc
     5641 aagcaaatta aagcagagga gatcctgccg tttttgaatc acaaattcca ggagccagtc
     5701 aagtattgga aaaacgggaa ggaagagagc ttcaatttaa cattattgat cgtcgaggca
     5761 gtccaagcac aggacaagcg taaacttcag ccctattatg actggaagac ctggtacatc
     5821 caaactaaaa gcgatttact gaagaagagt atcgagaaca atcgtatcga tttgactgag
     5881 aacttgtcaa agcgcaagaa ggcattgttg gcctgggaga cagagttcac agcatccggt
     5941 tcaatcgacc tgacgcatta ccataaggtc tatatgacgg atgttctgtg caaaatgtta
     6001 caagatgtta agccgctgac agatgataaa gggaagatca acaccaatgc gtatcatcgt
     6061 gggcttaaga aagccctgca gaaccaccaa ccagccatct ttggaacacg cgaggtgcct
     6121 aatgaggcta atcgtgcaga caatcagttg agcatttatc atttggaggt cgtgaagtat
     6181 cttgagcact attttccaat taaaacttcg aaacgccgca ataccgccga cgatatcgct
     6241 cattatttaa aggcacagac gttgaagaca acgattgaaa agcagctggt aaacgccatc
     6301 cgcgcgaaca tcatccaaca aggcaaaacc aatcatcacg agttgaaagc ggacacaaca
     6361 tcgaatgatt tgatccgtat taaaactaac gaagccttcg tcttaaactt aactggtacc
     6421 tgtgcgtttg cagcgaacaa tattcgcaac atggtggaca acgagcaaac taacgacatc
     6481 ctgggaaagg gggactttat caagagctta ctgaaggaca atacgaacag ccaattgtac
     6541 tcgttttttt ttggtgaagg attatccacg aataaggcag agaaagagac gcaactttgg
     6601 ggtatccgtg gggctgttca acagatccgc aataatgtaa atcattacaa gaaagacgcc
     6661 cttaaaaccg tgttcaacat ctcaaatttc gagaatccca ccattactga cccgaaacaa
     6721 cagactaact atgcagacac catctataaa gcgcgcttca tcaatgaatt ggaaaagatc
     6781 cctgaagcct ttgcacagca gcttaaaact ggcggtgctg tctcctatta cactatcgaa
     6841 aacctgaaat cattattgac tacgtttcag ttttcgctgt gtcgtagcac gattccattt
     6901 gcacctggtt tcaaaaaggt ttttaatgga ggaatcaatt accaaaacgc caagcaagat
     6961 gaatctttct atgagcttat gctggaacaa tacttgcgta aagagaattt cgccgaagag
     7021 agctacaatg cacgttattt tatgctgaag ttgatctata acaatctttt cctgccgggc
     7081 tttacgaccg accgtaaggc gttcgccgac agtgtgggat ttgtgcaaat gcagaacaaa
     7141 aagcaagctg agaaagtcaa ccctcgcaag aaagaggcgt atgcgttcga ggcggtacgt
     7201 ccgatgaccg cagcagactc tatcgcagat tacatggcat acgttcaaag tgaattaatg
     7261 caggagcaaa acaaaaagga ggaaaaagtt gccgaggaaa ctcgcatcaa ttttgaaaag
     7321 ttcgtccttc aggtgttcat taaagggttc gattcgttct tgcgcgccaa agagtttgac
     7381 ttcgtccaaa tgccacaacc tcaattgact gctacggcgt ccaaccaaca aaaagccgac
     7441 aagttaaacc aactggaagc ctctattact gctgattgca aacttacgcc ccaatatgcg
     7501 aaagcggacg atgctacaca tatcgcgttc tacgtctttt gcaaattact ggatgcggca
     7561 cacttgtcta atttgcgtaa cgagctgatt aagtttcgcg aatccgttaa cgaattcaaa
     7621 ttccaccatt tgctggaaat cattgagatt tgccttttat ctgcagatgt tgtgccaaca
     7681 gactatcgtg atctttatag tagcgaagct gactgcttag cacgtctgcg cccgttcatt
     7741 gaacagggcg cagacatcac aaactggtca gacttatttg tacaatctga caaacacagc
     7801 ccagtcatcc acgcaaacat tgagcttagt gttaaatatg gcacaactaa actgttagag
     7861 cagattatca ataaagatac tcaattcaag accacagagg caaattttac cgcctggaat
     7921 actgcgcaga agagcatcga acagcttatt aagcaacgcg aggaccatca cgagcaatgg
     7981 gtcaaggcta aaaacgccga cgacaaagaa aaacaagagc gcaaacgcga gaagtccaac
     8041 tttgcgcaaa agtttattga gaaacacggt gacgattatc ttgatatctg cgattatatc
     8101 aacacttata actggcttga caataaaatg cacttcgtgc acctgaaccg tctgcacggc
     8161 ttgactatcg agcttttagg tcgcatggcc gggtttgtcg ccctgtttga tcgcgatttt
     8221 caatttttcg atgagcaaca gatcgctgac gagttcaagt tacacggatt tgtgaactta
     8281 cacagtattg acaagaagct caatgaagtc cctactaaaa agatcaaaga gatctacgac
     8341 attcgtaaca aaatcattca aattaacgga aacaagatca atgagtccgt gcgtgccaac
     8401 ttaattcaat ttatctcgtc taaacgtaat tactataata acgccttctt gcacgtgtcc
     8461 aatgatgaga tcaaagaaaa gcagatgtat gatatccgca accatatcgc gcacttcaac
     8521 tatttgacga aggacgcggc tgacttctct ttgattgact tgattaacga gcttcgcgaa
     8581 ttgttacatt atgatcgcaa acttaagaac gctgtttcca aggcctttat cgacctgttt
     8641 gataagcacg gcatgattct gaagcttaag cttaacgcag accacaagtt aaaggttgag
     8701 tccctggagc caaagaaaat ttaccatctg ggatcatctg cgaaggacaa gccggagtat
     8761 caatattgca ccaaccaagt catgatggca tactgtaaca tgtgccgctc ccttttagag
     8821 atgaaaaaat aataacattg gaagtggata acggatccgc gatcgcggcg cgccacctgg
     8881 tggccggccg gtaccacgcg tgcgcgctga tccggctgct aacaaagccc gaaaggaagc
     8941 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     9001 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg
     9061 tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc
     9121 ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg
     9181 catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc
     9241 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
     9301 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
     9361 aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag
     9421 aa
//
