LOCUS       pBabe-tetoff-mVenus-PI-Cdc42        7200 bp    DNA     circular
            4-NOV-2011
ACCESSION   pBabe\pTRE\puro\lab\actual\81311
VERSION     pBabe\pTRE\puro\lab\actual\81311.0
KEYWORDS    .

COMMENT
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                <location x="5" y="134"/>
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            <element id="681788706" name="pBabe\R3"
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                <location x="5" y="134"/>
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            </element>
            <element id="1997524997" name="pbabe\out\3ltr\r1"
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                <location x="399" y="34"/>
            </element>
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                    <fill pattern="Solid">
                        <color blue="255" green="255" red="255"/>
                    </fill>
                </style>
                <location x="5" y="109"/>
                <dimension height="20" width="600"/>
            </element>
            <element id="1139536330"
                name="Tetracycline\responsive\element"
                target="Tetracycline\responsive\element:1364 - 1847"
                text="@N" type="29" xsi:type="label">
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                    <label text="@N">
                        <font name="Arial" size="9" style="1"/>
                        <color blue="0" green="0" red="0"/>
                    </label>
                </style>
                <location x="68" y="19"/>
            </element>
            <element id="1139536330"
                name="Tetracycline\responsive\element"
                target="Tetracycline\responsive\element:1364 - 1847"
                type="29" xsi:type="line">
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                    <line width="1">
                        <color blue="0" green="0" red="0"/>
                        <pattern style="1"/>
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            </element>
            <element id="1139536330"
                name="Tetracycline\responsive\element" shape="1"
                target="Tetracycline\responsive\element:1364 - 1847"
                type="29" xsi:type="symbol">
                <style name="Shape 1" shape="1" xsi:type="basicStyle">
                    <line width="1">
                        <color blue="102" green="153" red="0"/>
                        <pattern style="1"/>
                    </line>
                    <fill pattern="Solid">
                        <color blue="153" green="204" red="102"/>
                    </fill>
                </style>
                <location x="5" y="109"/>
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        </elements>
    </setupInfo>
    <setupInfo basesPerBlock="10" blocksPerLine="10"
        prefer1LetterAACode="true" preferSingleStrand="false"
        selected="true" textBold="false" textColor="0,0,0"
        textFontName="Andale Mono" textFontSize="14" textItalic="false"
        textUnderline="false" trackSeparatorColor="128,128,128"
        trackSeparatorWidth="2" type="Sequence"
        xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:type="sequenceSetup">
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    <setupInfo allowBlunt="true" allowFivePrime="true"
        allowThreePrime="true"
        enzymeMap="AscI|ggcgcgcc,AvaI|cycgrg,BsgI|gtgcag,ApaLI|gtgcac,BsrGI|tgtaca,BspEI|tccgga,NcoI|ccatgg,StuI|aggcct,EcoRI|gaattc,SmaI|cccggg,SalI|gtcgac,PstI|ctgcag,XhoI|ctcgag,SpeI|actagt,HindIII|aagctt,BamHI|ggatcc,XmaI|cccggg,ClaI|atcgat,MfeI|caattg,NheI|gctagc"
        enzymesCleavePoint="AscI|3,AvaI|2,BsgI|23,ApaLI|2,BsrGI|2,BspEI|2,NcoI|2,StuI|4,EcoRI|2,SmaI|4,SalI|2,PstI|6,XhoI|2,SpeI|2,HindIII|2,BamHI|2,XmaI|2,ClaI|3,MfeI|2,NheI|2"
        enzymesComplCleavePoint="AscI|0,AvaI|0,BsgI|21,ApaLI|0,BsrGI|0,BspEI|0,NcoI|0,StuI|0,EcoRI|0,SmaI|0,SalI|0,PstI|0,XhoI|0,SpeI|0,HindIII|0,BamHI|0,XmaI|0,ClaI|0,MfeI|0,NheI|0"
        enzymesPalindromic="AscI|true,AvaI|true,BsgI|false,ApaLI|true,BsrGI|true,BspEI|true,NcoI|true,StuI|true,EcoRI|true,SmaI|true,SalI|true,PstI|true,XhoI|true,SpeI|true,HindIII|true,BamHI|true,XmaI|true,ClaI|true,MfeI|true,NheI|true"
        ignoreIn="-1,-1" ignoreLess="-1" ignoreMore="-1"
        ignoreOut="-1,-1" selected="true" startIndex="1" type="Restriction Map"/>
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        startCodons="ATG GTG" stopCodons="TAA TGA TAG" type="ORFs"
        undefinedStart="false" undefinedStop="false" undefinedStopNested="false"/>
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DISPLAY_SETUP_END|
COMMENT     
            This file is created by Vector NTI
            http://www.invitrogen.com/
            VNTDATE|598373853|
            VNTDBDATE|605893729|
            LSOWNER|
            VNTNAME|pBabe pTRE puro lab actual 81311|
            VNTAUTHORNAME|Demo User|
            VNTSEQSTYLE|1853|1858||0|null|null|null|204,204,204|null
            VNTSEQSTYLE|3614|3664||0|null|null|null|255,204,102|null
            VNTSEQSTYLE|2576|2593||0|null|null|null|175,175,175|null
            VNTSEQSTYLE|1859|2575||0|null|null|null|255,255,0|null
            VNTSEQSTYLE|2735|3181||0|null|null|null|0,255,255|null
FEATURES             Location/Qualifiers
     primer          1316..1335
                     /vntifkey="27"
                     /label=pbabe\f1
     primer          4166..4185
                     /vntifkey="27"
                     /label=pBabeR2
     primer          1787..1806
                     /vntifkey="27"
                     /label=pbabe\f2
     primer          3716..3734
                     /vntifkey="27"
                     /label=pBabe\R3
     misc_feature    27..439
                     /vntifkey="21"
                     /label=5'\lTR
     misc_feature    4867..5145
                     /vntifkey="21"
                     /label=3'\LTR
     primer          4911..4931
                     /vntifkey="27"
                     /label=pbabe\3ltr\r1
     misc_feature    554..573
                     /vntifkey="21"
                     /label=pbabe\5'\lTR\F1
     primer          5146..5162
                     /vntifkey="27"
                     /label=pbabe\out\3ltr\r1
     primer          5341..5362
                     /vntifkey="27"
                     /label=out\3'\ltr\r2
     CDS             554..1362
                     /vntifkey="4"
                     /label=packaging\PSI\sequence
     promoter        1364..1847
                     /vntifkey="29"
                     /label=Tetracycline\responsive\element
     CDS             3642..4821
                     /vntifkey="4"
                     /label=Selection\Cassette
     misc_feature    1859..2575
                     /label=mVenus
                     /type="misc_feature"
                     /vntifkey="21"
     primer          1853..2595
                     /label=seq-pBabeFw
                     /type="primer"
                     /vntifkey="27"
     misc_feature    2621..3640
                     /label=PI-Cdc42
                     /type="misc_feature"
                     /vntifkey="21"
     misc_feature    2762..3208
                     /label=LOV2
                     /type="misc_feature"
                     /vntifkey="21"
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccac gaggcccttt
     1381 cgtcgtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     1441 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     1501 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     1561 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     1621 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     1681 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
     1741 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     1801 agacaccggg accgatccag cctccgcggc cccagatccg taaagtgaat tcgccaccat
     1861 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1921 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1981 caagctgacc ctgaagttga tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2041 cgtgaccacc ctcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca
     2101 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2161 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2221 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2281 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2341 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2401 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2461 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2521 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggttc
     2581 tggatccggt ggttctggtt ctggtggtac cggcgcgccg atgcagacaa ttaagtgtgt
     2641 tgttgtgggc gatggtgctg ttggtaaaac atgtctcctg atatcctaca caacaaacaa
     2701 atttccatcg gaatatgtac cgagtgtttt tgctaactat gcagtcacag ttatgattgg
     2761 tggatcaggt ttggctacta cacttgaacg tattgagaag aactttgtca ttactgaccc
     2821 aaggttgcca gataatccca ttatattcgc gtccgatagt ttcttgcagt tgacagaata
     2881 tagccgtgaa gaaattttgg gaagaaactg caggtttcta caaggtcctg aaactgatcg
     2941 cgcgacagtg agaaaaatta gagatgccat agataaccaa acagaggtca ctgttcagct
     3001 gattaattat acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc agcctatgcg
     3061 agatcagaag ggagatgtcc agtactttat tggggttcag ttggatggaa ctgagcatgt
     3121 ccgagatgct gccgagagag agggagtcat gctgattaag aaaactgcag aaaatattga
     3181 tgaggcggca aaagaacttg gctctggtgg agaaccatat actcttggac tttttgatac
     3241 tgcagggcta gaggattatg acagattacg accgctgagt tatccacaaa cagatgtatt
     3301 tctagtctgt ttttcagtgg tctctccatc ttcatttgaa aacgtgaaag aaaagtgggt
     3361 gcctgagata actcaccact gtccaaagac tcctttcttg cttgttggga ctcaaattga
     3421 tctcagagat gacccctcta ctattgagaa acttgccaag aacaaacaga agcctatcac
     3481 tccagagact gctgaaaagc tggcccgtga cctgaaggct gtcaagtatg tggagtgttc
     3541 tgcacttaca cagaaaggcc taaagaatgt atttgacgaa gcaatattgg ctgccctgga
     3601 gcctccagaa ccgaagaaga gccgcaggtg tgtgctgcta ctcgagtgtg gaattgtgag
     3661 cggataacaa tttgtcgagg gcccctgcag gtcaattcta ccgggtaggg gaggcgcttt
     3721 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3781 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3841 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3901 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3961 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4021 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4081 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4141 gcacgcttca aaagcgcaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     4201 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     4261 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     4321 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     4381 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     4441 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     4501 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4561 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4621 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4681 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4741 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     4801 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     4861 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     4921 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtag gagatccctg
     4981 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     5041 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     5101 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc
     5161 aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg
     5221 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5281 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5341 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5401 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5461 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5521 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5581 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5641 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5701 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5761 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5821 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5881 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5941 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6001 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6061 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6121 aaggatcttc acctagatcc ttttaaatta aaaatgaagt ttgcggccgc aaatcaatct
     6181 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6241 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6301 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6361 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6421 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6481 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6541 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6601 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6661 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6721 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6781 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     6841 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6901 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6961 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7021 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7081 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7141 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
