LOCUS       pC010_pLac_LshCas13a_lo 8486 bp ds-DNA     circular     25-MAR-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6410..6444
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6586..7776
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    6445..6472
                     /label="DR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    2240..6406
                     /label="LshCas13a"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6071..6073
                     /label="R1278"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4043..4045
                     /label="H602"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6086..6088
                     /label="H1283"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4028..4030
                     /label="R597"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6473..6500
                     /label="nontargeting spacer"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6501..6528
                     /label="DR"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tggggaacct gttcggacac aagcggtggt acgaggtgcg
     2281 agataagaaa gacttcaaga tcaagcgaaa ggtgaaagtc aagcggaatt atgacgggaa
     2341 caagtacatt ctgaacatca acgaaaacaa caacaaagag aagatcgaca acaacaagtt
     2401 catcagaaag tacatcaact acaagaaaaa cgataatatt ctgaaggagt tcactaggaa
     2461 atttcatgca ggaaatatcc tgttcaaact gaagggcaaa gaagggatca ttagaattga
     2521 gaataacgac gacttcctgg agacagagga agtggtgctg tatatcgagg cctacggcaa
     2581 gagcgagaag ctgaaagcac tggggatcac aaagaaaaag atcattgacg aggccatcag
     2641 gcagggcatt actaaggacg ataaaaagat cgagatcaag cgacaggaga acgaggaaga
     2701 gatcgaaatt gacatccggg atgagtacac taataagacc ctgaacgact gctccatcat
     2761 tctgcgcatc attgaaaacg atgaactgga gacaaagaag agcatctacg agatcttcaa
     2821 gaacatcaat atgagcctgt ataagatcat cgagaagatc atcgaaaacg agacagaaaa
     2881 ggtgtttgaa aatagatact acgaagagca cctgagggag aagctgctga aagacgataa
     2941 gattgacgtg atcctgacca acttcatgga aatccgggag aagatcaagt ctaatctgga
     3001 gatcctgggc ttcgtgaagt tttacctgaa cgtcggcggg gacaaaaaga aaagtaaaaa
     3061 taagaaaatg ctggtggaaa agattctgaa catcaatgtg gatctgaccg tcgaggacat
     3121 tgccgatttc gtgatcaagg agctggaatt ttggaacatc acaaagcgca ttgagaaagt
     3181 gaagaaagtc aataacgagt tcctggagaa gcggagaaat cggacataca tcaagtccta
     3241 tgtgctgctg gacaagcacg aaaagtttaa aatcgagaga gaaaacaaga aggataagat
     3301 cgtgaagttc tttgtcgaga acattaagaa caactctatc aaggaaaaga ttgagaagat
     3361 cctggctgag ttcaagatcg acgagctgat taagaaactg gagaaggaac tgaagaaagg
     3421 gaactgtgat accgagatct tcggaatctt taagaagcat tacaaggtga acttcgacag
     3481 caagaaattt tccaagaaat ctgatgaaga gaaggagctg tataagatca tctacagata
     3541 cctgaagggc agaattgaaa aaatcctggt gaacgagcag aaggtcagac tgaagaaaat
     3601 ggagaagatc gagatcgaaa agattctgaa tgaaagtatc ctgtcagaga aaattctgaa
     3661 gagagtgaaa cagtatacac tggagcacat tatgtacctg gggaagctga ggcataacga
     3721 catcgatatg accacagtga atactgacga tttcagccgc ctgcacgcca aggaagagct
     3781 ggacctggaa ctgatcacct tctttgccag cacaaatatg gagctgaaca agatcttttc
     3841 ccgagaaaac atcaacaacg acgagaacat cgatttcttt ggaggcgacc gggagaagaa
     3901 ctatgtgctg gataagaaaa tcctgaatag taagatcaag atcatccgcg acctggattt
     3961 catcgataac aagaacaaca tcacaaacaa cttcattcga aagtttacaa agatcggcac
     4021 taatgaaagg aaccgcatcc tgcatgccat ttccaaagag agggacctgc aggggactca
     4081 ggacgattac aacaaagtga tcaacatcat tcagaatctg aagatctccg atgaagaggt
     4141 gagcaaagct ctgaacctgg acgtggtctt taaggacaag aaaaacatca tcacaaagat
     4201 caatgacatc aagatctctg aagagaacaa caacgatatc aagtatctgc ccagcttcag
     4261 caaagtgctg cccgaaatcc tgaacctgta ccgcaacaat cccaagaatg agccttttga
     4321 cacaatcgag actgaaaaaa ttgtgctgaa cgctctgatc tacgtcaata aggagctgta
     4381 taagaaactg atcctggagg acgatctgga agagaacgag tccaagaata tcttcctgca
     4441 ggaactgaag aaaaccctgg gcaacattga cgaaatcgat gagaacatca tcgagaacta
     4501 ctacaagaac gcacagattt ctgccagtaa ggggaacaac aaggcaatca agaaatatca
     4561 gaagaaagtg atcgagtgct acattggata tctgcgcaaa aactacgaag agctgttcga
     4621 cttttcagac ttcaagatga acatccagga aatcaagaaa cagattaagg acatcaacga
     4681 taacaagact tatgagcgga tcaccgtgaa aaccagcgac aaaaccattg tcatcaacga
     4741 cgatttcgag tacatcattt ctatctttgc actgctgaac agtaatgccg tgattaataa
     4801 gatccgaaac agattcttcg ccaccagcgt gtggctgaac acctcagaat accagaatat
     4861 cattgacatc ctggatgaga ttatgcagct gaataccctg cggaacgaat gcatcacaga
     4921 gaactggaat ctgaacctgg aagagttcat tcagaagatg aaagagatcg aaaaggattt
     4981 cgacgacttc aagatccaga ctaagaaaga aatcttcaac aactactacg aggacatcaa
     5041 gaacaacatt ctgaccgagt ttaaagacga tatcaacggc tgtgatgtgc tggaaaagaa
     5101 actggagaag attgtcatct tcgacgatga aaccaagttc gagatcgaca agaaatccaa
     5161 catcctgcag gatgaacaga gaaagctgtc taacatcaac aagaaggacc tgaagaaaaa
     5221 ggtggatcag tatatcaagg acaaagatca ggagatcaag tctaaaatcc tgtgcaggat
     5281 cattttcaac agtgactttc tgaaaaagta caagaaggaa atcgacaatc tgattgagga
     5341 tatggagtct gaaaatgaga acaagttcca ggagatctac tatcccaagg aacggaagaa
     5401 cgagctgtat atctacaaaa agaatctgtt cctgaacatc ggaaatccta actttgacaa
     5461 gatctacggc ctgattagca acgacatcaa gatggccgat gctaaattcc tgtttaatat
     5521 cgatggaaag aacatcagaa agaacaaaat cagtgagatc gacgctattc tgaagaatct
     5581 gaacgataaa ctgaacggct actcaaagga atacaaggag aagtacatca aaaagctgaa
     5641 ggagaacgac gatttctttg caaagaacat ccagaataag aactacaaat ccttcgaaaa
     5701 ggactataac cgcgtgtctg agtacaaaaa gattcgagat ctggtcgagt tcaactatct
     5761 gaacaaaatc gagtcctacc tgattgacat caactggaag ctggctattc agatggcaag
     5821 attcgaaagg gatatgcact atatcgtgaa tggactgagg gagctgggca tcattaagct
     5881 gtcaggctat aacaccggga tcagcagggc atacccaaag cgcaatggaa gcgacggctt
     5941 ttacactacc acagcctact acaagttctt tgatgaagag tcctacaaga agttcgagaa
     6001 gatttgctac gggtttggaa tcgacctgag cgaaaattcc gagatcaaca agcctgaaaa
     6061 tgagagcatt cggaactata tctcccattt ctacatcgtg agaaatccat ttgccgacta
     6121 cagtattgct gagcagatcg atcgggtgag caacctgctg tcatatagca cacgctacaa
     6181 caattcaact tatgccagcg tgttcgaagt ctttaaaaag gacgtgaatc tggactacga
     6241 tgagctgaaa aagaaattca aactgatcgg caacaatgat attctggagc gcctgatgaa
     6301 gcccaagaaa gtgagcgtcc tggaactgga gtcctacaac agtgactaca ttaagaatct
     6361 gatcattgaa ctgctgacca aaatcgagaa tactaacgat accctgTGAT TGACAGCTAG
     6421 CTCAGTCCTA GGTATAATGC TAGCCCACCC CAATATCGAA GGGGACTAAA ACtatggatt
     6481 acttggtaga ACAGCAATCT CCACCCCAAT ATCGAAGGGG ACTAAAACaa gctttaatgc
     6541 ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat
     6601 gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
     6661 ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat
     6721 ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca
     6781 ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc
     6841 gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg
     6901 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     6961 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     7021 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     7081 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     7141 aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     7201 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     7261 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     7321 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     7381 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     7441 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     7501 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     7561 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     7621 ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
     7681 tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
     7741 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
     7801 acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     7861 aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     7921 gcatctcggg cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga
     7981 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
     8041 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
     8101 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcccctacg tgctgctgaa
     8161 gttgcccgca acagagagtg gaaccaaccg gtgataccac gatactatga ctgagagtca
     8221 acgccatgag cggcctcatt tcttattctg agttacaaca gtccgcaccg ctgtccggta
     8281 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     8341 tcatgcaact cgtaggacag gtgccggcag cgcccaacag tcccccggcc acggggcctg
     8401 ccaccatacc cacgccgaaa caagcgccct gcaccattat gttccggatc tgcatcgcag
     8461 gatgctgctg gctaccctgt ggaaca
//