LOCUS pC013_pET_His6-TwinStre 9542 bp ds-DNA circular 25-MAR-2017 DEFINITION . ACCESSION pR0046 His6-TwinStrep- VERSION pR0046 His6-TwinStrep- SOURCE synthetic DNA construct ORGANISM recombinant plasmid . FEATURES Location/Qualifiers primer_bind 2743..2762 /label="T7" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS complement(1900..2559) /label="AmpR" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 2983..3274 /label="Sumo" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(1120..1802) /label="ColE1 origin" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_binding 2762..2784 /label="LacO" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 3280..6735 /label="LwCas13a" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2834..2982 /label="6x His tag/ Twin Strep" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 ORIGIN 1 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac 61 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg 121 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 181 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 241 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 301 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 361 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 421 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 481 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 601 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1021 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 1081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1321 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 1381 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 1441 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 1501 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 1561 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 1621 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 1681 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 1741 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 1801 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 1861 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 1921 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 1981 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 2041 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 2101 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 2161 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 2221 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 2281 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 2341 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 2401 aggcgtatca cgaggccctt tcgtcttcaa gaaccgggat ctcgacgctc tcccttatgc 2461 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca 2521 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc 2581 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg 2641 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg 2701 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag 2761 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg 2821 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc 2881 ggcagccata tggctagctg gagccatccg cagtttgaaa aaggtggtgg tagcggtggt 2941 ggttcaggtg gtagtgcatg gtcacaccct cagtttgaga aaatgtcgga ctcagaagtc 3001 aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta 3061 aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga 3121 aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg 3181 tacgacggta ttagaatcca agctgatcag acccctgaag atttggacat ggaggataac 3241 gatattattg aggctcacag agaacagatt ggtggatccA TGAAAGTGAC CAAGGTCGAC 3301 GGCATCAGCC ACAAGAAGTA CATCGAAGAG GGCAAGCTCG TGAAGTCCAC CAGCGAGGAA 3361 AACCGGACCA GCGAGAGACT GAGCGAGCTG CTGAGCATCC GGCTGGACAT CTACATCAAG 3421 AACCCCGACA ACGCCTCCGA GGAAGAGAAC CGGATCAGAA GAGAGAACCT GAAGAAGTTC 3481 TTTAGCAACA AGGTGCTGCA CCTGAAGGAC AGCGTGCTGT ATCTGAAGAA CCGGAAAGAA 3541 AAGAACGCCG TGCAGGACAA GAACTATAGC GAAGAGGACA TCAGCGAGTA CGACCTGAAA 3601 AACAAGAACA GCTTCTCCGT GCTGAAGAAG ATCCTGCTGA ACGAGGACGT GAACTCTGAG 3661 GAACTGGAAA TCTTTCGGAA GGACGTGGAA GCCAAGCTGA ACAAGATCAA CAGCCTGAAG 3721 TACAGCTTCG AAGAGAACAA GGCCAACTAC CAGAAGATCA ACGAGAACAA CGTGGAAAAA 3781 GTGGGCGGCA AGAGCAAGCG GAACATCATC TACGACTACT ACAGAGAGAG CGCCAAGCGC 3841 AACGACTACA TCAACAACGT GCAGGAAGCC TTCGACAAGC TGTATAAGAA AGAGGATATC 3901 GAGAAACTGT TTTTCCTGAT CGAGAACAGC AAGAAGCACG AGAAGTACAA GATCCGCGAG 3961 TACTATCACA AGATCATCGG CCGGAAGAAC GACAAAGAGA ACTTCGCCAA GATTATCTAC 4021 GAAGAGATCC AGAACGTGAA CAACATCAAA GAGCTGATTG AGAAGATCCC CGACATGTCT 4081 GAGCTGAAGA AAAGCCAGGT GTTCTACAAG TACTACCTGG ACAAAGAGGA ACTGAACGAC 4141 AAGAATATTA AGTACGCCTT CTGCCACTTC GTGGAAATCG AGATGTCCCA GCTGCTGAAA 4201 AACTACGTGT ACAAGCGGCT GAGCAACATC AGCAACGATA AGATCAAGCG GATCTTCGAG 4261 TACCAGAATC TGAAAAAGCT GATCGAAAAC AAACTGCTGA ACAAGCTGGA CACCTACGTG 4321 CGGAACTGCG GCAAGTACAA CTACTATCTG CAAGTGGGCG AGATCGCCAC CTCCGACTTT 4381 ATCGCCCGGA ACCGGCAGAA CGAGGCCTTC CTGAGAAACA TCATCGGCGT GTCCAGCGTG 4441 GCCTACTTCA GCCTGAGGAA CATCCTGGAA ACCGAGAACG AGAACGGTAT CACCGGCCGG 4501 ATGCGGGGCA AGACCGTGAA GAACAACAAG GGCGAAGAGA AATACGTGTC CGGCGAGGTG 4561 GACAAGATCT ACAATGAGAA CAAGCAGAAC GAAGTGAAAG AAAATCTGAA GATGTTCTAC 4621 AGCTACGACT TCAACATGGA CAACAAGAAC GAGATCGAGG ACTTCTTCGC CAACATCGAC 4681 GAGGCCATCA GCAGCATCAG ACACGGCATC GTGCACTTCA ACCTGGAACT GGAAGGCAAG 4741 GACATCTTCG CCTTCAAGAA TATCGCCCCC AGCGAGATCT CCAAGAAGAT GTTTCAGAAC 4801 GAAATCAACG AAAAGAAGCT GAAGCTGAAA ATCTTCAAGC AGCTGAACAG CGCCAACGTG 4861 TTCAACTACT ACGAGAAGGA TGTGATCATC AAGTACCTGA AGAATACCAA GTTCAACTTC 4921 GTGAACAAAA ACATCCCCTT CGTGCCCAGC TTCACCAAGC TGTACAACAA GATTGAGGAC 4981 CTGCGGAATA CCCTGAAGTT TTTTTGGAGC GTGCCCAAGG ACAAAGAAGA GAAGGACGCC 5041 CAGATCTACC TGCTGAAGAA TATCTACTAC GGCGAGTTCC TGAACAAGTT CGTGAAAAAC 5101 TCCAAGGTGT TCTTTAAGAT CACCAATGAA GTGATCAAGA TTAACAAGCA GCGGAACCAG 5161 AAAACCGGCC ACTACAAGTA TCAGAAGTTC GAGAACATCG AGAAAACCGT GCCCGTGGAA 5221 TACCTGGCCA TCATCCAGAG CAGAGAGATG ATCAACAACC AGGACAAAGA GGAAAAGAAT 5281 ACCTACATCG ACTTTATTCA GCAGATTTTC CTGAAGGGCT TCATCGACTA CCTGAACAAG 5341 AACAATCTGA AGTATATCGA GAGCAACAAC AACAATGACA ACAACGACAT CTTCTCCAAG 5401 ATCAAGATCA AAAAGGATAA CAAAGAGAAG TACGACAAGA TCCTGAAGAA CTATGAGAAG 5461 CACAATCGGA ACAAAGAAAT CCCTCACGAG ATCAATGAGT TCGTGCGCGA GATCAAGCTG 5521 GGGAAGATTC TGAAGTACAC CGAGAATCTG AACATGTTTT ACCTGATCCT GAAGCTGCTG 5581 AACCACAAAG AGCTGACCAA CCTGAAGGGC AGCCTGGAAA AGTACCAGTC CGCCAACAAA 5641 GAAGAAACCT TCAGCGACGA GTTGGAACTG ATCAACCTGC TGAACCTGGA CAACAACAGA 5701 GTGACCGAGG ACTTCGAGCT GGAAGCCAAC GAGATCGGCA AGTTCCTGGA CTTCAACGAA 5761 AACAAAATCA AGGACCGGAA AGAGCTGAAA AAGTTCGACA CCAACAAGAT CTATTTCGAC 5821 GGCGAGAACA TCATCAAGCA CCGGGCCTTC TACAATATCA AGAAATACGG CATGCTGAAT 5881 CTGCTGGAAA AGATCGCCGA TAAGGCCAAG TATAAGATCA GCCTGAAAGA ACTGAAAGAG 5941 TACAGCAACA AGAAGAATGA GATTGAAAAG AACTACACCA TGCAGCAGAA CCTGCACCGG 6001 AAGTACGCCA GACCCAAGAA GGACGAAAAG TTCAACGACG AGGACTACAA AGAGTATGAG 6061 AAGGCCATCG GCAACATCCA GAAGTACACC CACCTGAAGA ACAAGGTGGA ATTCAATGAG 6121 CTGAACCTGC TGCAGGGCCT GCTGCTGAAG ATCCTGCACC GGCTCGTGGG CTACACCAGC 6181 ATCTGGGAGC GGGACCTGAG ATTCCGGCTG AAGGGCGAGT TTCCCGAGAA CCACTACATC 6241 GAGGAAATTT TCAATTTCGA CAACTCCAAG AATGTGAAGT ACAAAAGCGG CCAGATCGTG 6301 GAAAAGTATA TCAACTTCTA CAAAGAACTG TACAAGGACA ATGTGGAAAA GCGGAGCATC 6361 TACTCCGACA AGAAAGTGAA GAAACTGAAG CAGGAAAAAA AGGACCTGTA CATCCGGAAC 6421 TACATTGCCC ACTTCAACTA CATCCCCCAC GCCGAGATTA GCCTGCTGGA AGTGCTGGAA 6481 AACCTGCGGA AGCTGCTGTC CTACGACCGG AAGCTGAAGA ACGCCATCAT GAAGTCCATC 6541 GTGGACATTC TGAAAGAATA CGGCTTCGTG GCCACCTTCA AGATCGGCGC TGACAAGAAG 6601 ATCGAAATCC AGACCCTGGA ATCAGAGAAG ATCGTGCACC TGAAGAATCT GAAGAAAAAG 6661 AAACTGATGA CCGACCGGAA CAGCGAGGAA CTGTGCGAAC TCGTGAAAGT CATGTTCGAG 6721 TACAAGGCCC TGGAAgcggc cgcactcgag gcccgaaagg aagctgagtt ggctgctgcc 6781 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt 6841 ttgctgaaag gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa 6901 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt 6961 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt 7021 aaagcttatc gatgataagc tgtcaaacat gagaaacacc atcgaatggc gcaaaacctt 7081 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 7141 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 7201 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 7261 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 7321 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 7381 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 7441 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 7501 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 7561 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 7621 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 7681 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 7741 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 7801 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 7861 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 7921 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 7981 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 8041 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 8101 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 8161 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 8221 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 8281 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 8341 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 8401 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 8461 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 8521 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 8581 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 8641 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 8701 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg 8761 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 8821 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 8881 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 8941 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 9001 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 9061 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 9121 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 9181 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 9241 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 9301 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 9361 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 9421 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 9481 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 9541 aa //