LOCUS       pC013_pET_His6-TwinStre 9542 bp ds-DNA     circular     25-MAR-2017
DEFINITION  .
ACCESSION   pR0046 His6-TwinStrep-                                             
VERSION     pR0046 His6-TwinStrep-                                             
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid .                                              
FEATURES             Location/Qualifiers
     primer_bind     2743..2762
                     /label="T7"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(1900..2559)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2983..3274
                     /label="Sumo"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1120..1802)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_binding    2762..2784
                     /label="LacO"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3280..6735
                     /label="LwCas13a"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2834..2982
                     /label="6x His tag/ Twin Strep"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
       61 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
      121 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
      181 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
      241 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
      301 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
      361 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      421 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      481 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      601 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1021 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     1081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1321 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1381 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1441 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1501 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1561 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1621 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1681 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1741 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1801 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1861 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1921 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1981 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2041 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2101 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2161 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2221 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2281 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2341 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2401 aggcgtatca cgaggccctt tcgtcttcaa gaaccgggat ctcgacgctc tcccttatgc
     2461 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     2521 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     2581 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     2641 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     2701 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
     2761 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
     2821 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc
     2881 ggcagccata tggctagctg gagccatccg cagtttgaaa aaggtggtgg tagcggtggt
     2941 ggttcaggtg gtagtgcatg gtcacaccct cagtttgaga aaatgtcgga ctcagaagtc
     3001 aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta
     3061 aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga
     3121 aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg
     3181 tacgacggta ttagaatcca agctgatcag acccctgaag atttggacat ggaggataac
     3241 gatattattg aggctcacag agaacagatt ggtggatccA TGAAAGTGAC CAAGGTCGAC
     3301 GGCATCAGCC ACAAGAAGTA CATCGAAGAG GGCAAGCTCG TGAAGTCCAC CAGCGAGGAA
     3361 AACCGGACCA GCGAGAGACT GAGCGAGCTG CTGAGCATCC GGCTGGACAT CTACATCAAG
     3421 AACCCCGACA ACGCCTCCGA GGAAGAGAAC CGGATCAGAA GAGAGAACCT GAAGAAGTTC
     3481 TTTAGCAACA AGGTGCTGCA CCTGAAGGAC AGCGTGCTGT ATCTGAAGAA CCGGAAAGAA
     3541 AAGAACGCCG TGCAGGACAA GAACTATAGC GAAGAGGACA TCAGCGAGTA CGACCTGAAA
     3601 AACAAGAACA GCTTCTCCGT GCTGAAGAAG ATCCTGCTGA ACGAGGACGT GAACTCTGAG
     3661 GAACTGGAAA TCTTTCGGAA GGACGTGGAA GCCAAGCTGA ACAAGATCAA CAGCCTGAAG
     3721 TACAGCTTCG AAGAGAACAA GGCCAACTAC CAGAAGATCA ACGAGAACAA CGTGGAAAAA
     3781 GTGGGCGGCA AGAGCAAGCG GAACATCATC TACGACTACT ACAGAGAGAG CGCCAAGCGC
     3841 AACGACTACA TCAACAACGT GCAGGAAGCC TTCGACAAGC TGTATAAGAA AGAGGATATC
     3901 GAGAAACTGT TTTTCCTGAT CGAGAACAGC AAGAAGCACG AGAAGTACAA GATCCGCGAG
     3961 TACTATCACA AGATCATCGG CCGGAAGAAC GACAAAGAGA ACTTCGCCAA GATTATCTAC
     4021 GAAGAGATCC AGAACGTGAA CAACATCAAA GAGCTGATTG AGAAGATCCC CGACATGTCT
     4081 GAGCTGAAGA AAAGCCAGGT GTTCTACAAG TACTACCTGG ACAAAGAGGA ACTGAACGAC
     4141 AAGAATATTA AGTACGCCTT CTGCCACTTC GTGGAAATCG AGATGTCCCA GCTGCTGAAA
     4201 AACTACGTGT ACAAGCGGCT GAGCAACATC AGCAACGATA AGATCAAGCG GATCTTCGAG
     4261 TACCAGAATC TGAAAAAGCT GATCGAAAAC AAACTGCTGA ACAAGCTGGA CACCTACGTG
     4321 CGGAACTGCG GCAAGTACAA CTACTATCTG CAAGTGGGCG AGATCGCCAC CTCCGACTTT
     4381 ATCGCCCGGA ACCGGCAGAA CGAGGCCTTC CTGAGAAACA TCATCGGCGT GTCCAGCGTG
     4441 GCCTACTTCA GCCTGAGGAA CATCCTGGAA ACCGAGAACG AGAACGGTAT CACCGGCCGG
     4501 ATGCGGGGCA AGACCGTGAA GAACAACAAG GGCGAAGAGA AATACGTGTC CGGCGAGGTG
     4561 GACAAGATCT ACAATGAGAA CAAGCAGAAC GAAGTGAAAG AAAATCTGAA GATGTTCTAC
     4621 AGCTACGACT TCAACATGGA CAACAAGAAC GAGATCGAGG ACTTCTTCGC CAACATCGAC
     4681 GAGGCCATCA GCAGCATCAG ACACGGCATC GTGCACTTCA ACCTGGAACT GGAAGGCAAG
     4741 GACATCTTCG CCTTCAAGAA TATCGCCCCC AGCGAGATCT CCAAGAAGAT GTTTCAGAAC
     4801 GAAATCAACG AAAAGAAGCT GAAGCTGAAA ATCTTCAAGC AGCTGAACAG CGCCAACGTG
     4861 TTCAACTACT ACGAGAAGGA TGTGATCATC AAGTACCTGA AGAATACCAA GTTCAACTTC
     4921 GTGAACAAAA ACATCCCCTT CGTGCCCAGC TTCACCAAGC TGTACAACAA GATTGAGGAC
     4981 CTGCGGAATA CCCTGAAGTT TTTTTGGAGC GTGCCCAAGG ACAAAGAAGA GAAGGACGCC
     5041 CAGATCTACC TGCTGAAGAA TATCTACTAC GGCGAGTTCC TGAACAAGTT CGTGAAAAAC
     5101 TCCAAGGTGT TCTTTAAGAT CACCAATGAA GTGATCAAGA TTAACAAGCA GCGGAACCAG
     5161 AAAACCGGCC ACTACAAGTA TCAGAAGTTC GAGAACATCG AGAAAACCGT GCCCGTGGAA
     5221 TACCTGGCCA TCATCCAGAG CAGAGAGATG ATCAACAACC AGGACAAAGA GGAAAAGAAT
     5281 ACCTACATCG ACTTTATTCA GCAGATTTTC CTGAAGGGCT TCATCGACTA CCTGAACAAG
     5341 AACAATCTGA AGTATATCGA GAGCAACAAC AACAATGACA ACAACGACAT CTTCTCCAAG
     5401 ATCAAGATCA AAAAGGATAA CAAAGAGAAG TACGACAAGA TCCTGAAGAA CTATGAGAAG
     5461 CACAATCGGA ACAAAGAAAT CCCTCACGAG ATCAATGAGT TCGTGCGCGA GATCAAGCTG
     5521 GGGAAGATTC TGAAGTACAC CGAGAATCTG AACATGTTTT ACCTGATCCT GAAGCTGCTG
     5581 AACCACAAAG AGCTGACCAA CCTGAAGGGC AGCCTGGAAA AGTACCAGTC CGCCAACAAA
     5641 GAAGAAACCT TCAGCGACGA GTTGGAACTG ATCAACCTGC TGAACCTGGA CAACAACAGA
     5701 GTGACCGAGG ACTTCGAGCT GGAAGCCAAC GAGATCGGCA AGTTCCTGGA CTTCAACGAA
     5761 AACAAAATCA AGGACCGGAA AGAGCTGAAA AAGTTCGACA CCAACAAGAT CTATTTCGAC
     5821 GGCGAGAACA TCATCAAGCA CCGGGCCTTC TACAATATCA AGAAATACGG CATGCTGAAT
     5881 CTGCTGGAAA AGATCGCCGA TAAGGCCAAG TATAAGATCA GCCTGAAAGA ACTGAAAGAG
     5941 TACAGCAACA AGAAGAATGA GATTGAAAAG AACTACACCA TGCAGCAGAA CCTGCACCGG
     6001 AAGTACGCCA GACCCAAGAA GGACGAAAAG TTCAACGACG AGGACTACAA AGAGTATGAG
     6061 AAGGCCATCG GCAACATCCA GAAGTACACC CACCTGAAGA ACAAGGTGGA ATTCAATGAG
     6121 CTGAACCTGC TGCAGGGCCT GCTGCTGAAG ATCCTGCACC GGCTCGTGGG CTACACCAGC
     6181 ATCTGGGAGC GGGACCTGAG ATTCCGGCTG AAGGGCGAGT TTCCCGAGAA CCACTACATC
     6241 GAGGAAATTT TCAATTTCGA CAACTCCAAG AATGTGAAGT ACAAAAGCGG CCAGATCGTG
     6301 GAAAAGTATA TCAACTTCTA CAAAGAACTG TACAAGGACA ATGTGGAAAA GCGGAGCATC
     6361 TACTCCGACA AGAAAGTGAA GAAACTGAAG CAGGAAAAAA AGGACCTGTA CATCCGGAAC
     6421 TACATTGCCC ACTTCAACTA CATCCCCCAC GCCGAGATTA GCCTGCTGGA AGTGCTGGAA
     6481 AACCTGCGGA AGCTGCTGTC CTACGACCGG AAGCTGAAGA ACGCCATCAT GAAGTCCATC
     6541 GTGGACATTC TGAAAGAATA CGGCTTCGTG GCCACCTTCA AGATCGGCGC TGACAAGAAG
     6601 ATCGAAATCC AGACCCTGGA ATCAGAGAAG ATCGTGCACC TGAAGAATCT GAAGAAAAAG
     6661 AAACTGATGA CCGACCGGAA CAGCGAGGAA CTGTGCGAAC TCGTGAAAGT CATGTTCGAG
     6721 TACAAGGCCC TGGAAgcggc cgcactcgag gcccgaaagg aagctgagtt ggctgctgcc
     6781 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     6841 ttgctgaaag gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa
     6901 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     6961 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     7021 aaagcttatc gatgataagc tgtcaaacat gagaaacacc atcgaatggc gcaaaacctt
     7081 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     7141 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     7201 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     7261 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
     7321 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     7381 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     7441 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     7501 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     7561 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     7621 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     7681 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     7741 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     7801 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     7861 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     7921 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     7981 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     8041 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     8101 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8161 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8221 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
     8281 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     8341 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     8401 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     8461 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     8521 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     8581 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     8641 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     8701 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg
     8761 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     8821 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     8881 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     8941 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
     9001 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc
     9061 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
     9121 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     9181 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     9241 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     9301 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     9361 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     9421 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     9481 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     9541 aa
//