LOCUS       pR0035.1_pLac_C2C2.2    8478 bp ds-DNA     circular     13-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature      1208..1348
                       /label="part of IS1 from Tn9"
                       /ApEinfo_revcolor=#f8d3a9
                       /ApEinfo_fwdcolor=#f8d3a9
     Catalytic residue 4028..4030
                       /label="R597"
                       /ApEinfo_revcolor=#d59687
                       /ApEinfo_fwdcolor=#d59687
     misc_feature      2196..2225
                       /label="lac promoter"
                       /ApEinfo_revcolor=#7ea7d8
                       /ApEinfo_fwdcolor=#7ea7d8
     misc_feature      6578..7768
                       /label="TcR"
                       /ApEinfo_revcolor=#d59687
                       /ApEinfo_fwdcolor=#d59687
     misc_feature      6473..6492
                       /label="BsmBI insertion site"
                       /ApEinfo_revcolor=#9eafd2
                       /ApEinfo_fwdcolor=#9eafd2
     misc_feature      6493..6520
                       /label="DR"
                       /ApEinfo_revcolor=#b4abac
                       /ApEinfo_fwdcolor=#b4abac
     misc_feature      2226..2231
                       /label="Shine-Dalgarno sequence"
                       /ApEinfo_revcolor=#7ea7d8
                       /ApEinfo_fwdcolor=#7ea7d8
     misc_feature      1613..2158
                       /label="ori"
                       /ApEinfo_revcolor=#c7b0e3
                       /ApEinfo_fwdcolor=#c7b0e3
     misc_feature      6445..6472
                       /label="DR"
                       /ApEinfo_revcolor=#b1ff67
                       /ApEinfo_fwdcolor=#b1ff67
     CDS               complement(328..984)
                       /label="CmR"
                       /ApEinfo_revcolor=#fff79a
                       /ApEinfo_fwdcolor=#fff79a
     Catalytic residue 6071..6073
                       /label="R1278"
                       /ApEinfo_revcolor=#ff9ccd
                       /ApEinfo_fwdcolor=#ff9ccd
     misc_feature      2232..2239
                       /label="Eco31I recognition and cut site"
                       /ApEinfo_revcolor=#7ea7d8
                       /ApEinfo_fwdcolor=#7ea7d8
     Catalytic residue 4043..4045
                       /label="H602"
                       /ApEinfo_revcolor=#84b0dc
                       /ApEinfo_fwdcolor=#84b0dc
     Catalytic residue 6086..6088
                       /label="H1283"
                       /ApEinfo_revcolor=#ffef86
                       /ApEinfo_fwdcolor=#ffef86
     misc_feature      6410..6444
                       /label="J23119 Promoter"
                       /ApEinfo_revcolor=#84b0dc
                       /ApEinfo_fwdcolor=#84b0dc
     misc_feature      2240..6406
                       /label="Lsh C2c2"
                       /ApEinfo_revcolor=#ff9ccd
                       /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tggggaacct gttcggacac aagcggtggt acgaggtgcg
     2281 agataagaaa gacttcaaga tcaagcgaaa ggtgaaagtc aagcggaatt atgacgggaa
     2341 caagtacatt ctgaacatca acgaaaacaa caacaaagag aagatcgaca acaacaagtt
     2401 catcagaaag tacatcaact acaagaaaaa cgataatatt ctgaaggagt tcactaggaa
     2461 atttcatgca ggaaatatcc tgttcaaact gaagggcaaa gaagggatca ttagaattga
     2521 gaataacgac gacttcctgg agacagagga agtggtgctg tatatcgagg cctacggcaa
     2581 gagcgagaag ctgaaagcac tggggatcac aaagaaaaag atcattgacg aggccatcag
     2641 gcagggcatt actaaggacg ataaaaagat cgagatcaag cgacaggaga acgaggaaga
     2701 gatcgaaatt gacatccggg atgagtacac taataagacc ctgaacgact gctccatcat
     2761 tctgcgcatc attgaaaacg atgaactgga gacaaagaag agcatctacg agatcttcaa
     2821 gaacatcaat atgagcctgt ataagatcat cgagaagatc atcgaaaacg agacagaaaa
     2881 ggtgtttgaa aatagatact acgaagagca cctgagggag aagctgctga aagacgataa
     2941 gattgacgtg atcctgacca acttcatgga aatccgggag aagatcaagt ctaatctgga
     3001 gatcctgggc ttcgtgaagt tttacctgaa cgtcggcggg gacaaaaaga aaagtaaaaa
     3061 taagaaaatg ctggtggaaa agattctgaa catcaatgtg gatctgaccg tcgaggacat
     3121 tgccgatttc gtgatcaagg agctggaatt ttggaacatc acaaagcgca ttgagaaagt
     3181 gaagaaagtc aataacgagt tcctggagaa gcggagaaat cggacataca tcaagtccta
     3241 tgtgctgctg gacaagcacg aaaagtttaa aatcgagaga gaaaacaaga aggataagat
     3301 cgtgaagttc tttgtcgaga acattaagaa caactctatc aaggaaaaga ttgagaagat
     3361 cctggctgag ttcaagatcg acgagctgat taagaaactg gagaaggaac tgaagaaagg
     3421 gaactgtgat accgagatct tcggaatctt taagaagcat tacaaggtga acttcgacag
     3481 caagaaattt tccaagaaat ctgatgaaga gaaggagctg tataagatca tctacagata
     3541 cctgaagggc agaattgaaa aaatcctggt gaacgagcag aaggtcagac tgaagaaaat
     3601 ggagaagatc gagatcgaaa agattctgaa tgaaagtatc ctgtcagaga aaattctgaa
     3661 gagagtgaaa cagtatacac tggagcacat tatgtacctg gggaagctga ggcataacga
     3721 catcgatatg accacagtga atactgacga tttcagccgc ctgcacgcca aggaagagct
     3781 ggacctggaa ctgatcacct tctttgccag cacaaatatg gagctgaaca agatcttttc
     3841 ccgagaaaac atcaacaacg acgagaacat cgatttcttt ggaggcgacc gggagaagaa
     3901 ctatgtgctg gataagaaaa tcctgaatag taagatcaag atcatccgcg acctggattt
     3961 catcgataac aagaacaaca tcacaaacaa cttcattcga aagtttacaa agatcggcac
     4021 taatgaaagg aaccgcatcc tgcatgccat ttccaaagag agggacctgc aggggactca
     4081 ggacgattac aacaaagtga tcaacatcat tcagaatctg aagatctccg atgaagaggt
     4141 gagcaaagct ctgaacctgg acgtggtctt taaggacaag aaaaacatca tcacaaagat
     4201 caatgacatc aagatctctg aagagaacaa caacgatatc aagtatctgc ccagcttcag
     4261 caaagtgctg cccgaaatcc tgaacctgta ccgcaacaat cccaagaatg agccttttga
     4321 cacaatcgag actgaaaaaa ttgtgctgaa cgctctgatc tacgtcaata aggagctgta
     4381 taagaaactg atcctggagg acgatctgga agagaacgag tccaagaata tcttcctgca
     4441 ggaactgaag aaaaccctgg gcaacattga cgaaatcgat gagaacatca tcgagaacta
     4501 ctacaagaac gcacagattt ctgccagtaa ggggaacaac aaggcaatca agaaatatca
     4561 gaagaaagtg atcgagtgct acattggata tctgcgcaaa aactacgaag agctgttcga
     4621 cttttcagac ttcaagatga acatccagga aatcaagaaa cagattaagg acatcaacga
     4681 taacaagact tatgagcgga tcaccgtgaa aaccagcgac aaaaccattg tcatcaacga
     4741 cgatttcgag tacatcattt ctatctttgc actgctgaac agtaatgccg tgattaataa
     4801 gatccgaaac agattcttcg ccaccagcgt gtggctgaac acctcagaat accagaatat
     4861 cattgacatc ctggatgaga ttatgcagct gaataccctg cggaacgaat gcatcacaga
     4921 gaactggaat ctgaacctgg aagagttcat tcagaagatg aaagagatcg aaaaggattt
     4981 cgacgacttc aagatccaga ctaagaaaga aatcttcaac aactactacg aggacatcaa
     5041 gaacaacatt ctgaccgagt ttaaagacga tatcaacggc tgtgatgtgc tggaaaagaa
     5101 actggagaag attgtcatct tcgacgatga aaccaagttc gagatcgaca agaaatccaa
     5161 catcctgcag gatgaacaga gaaagctgtc taacatcaac aagaaggacc tgaagaaaaa
     5221 ggtggatcag tatatcaagg acaaagatca ggagatcaag tctaaaatcc tgtgcaggat
     5281 cattttcaac agtgactttc tgaaaaagta caagaaggaa atcgacaatc tgattgagga
     5341 tatggagtct gaaaatgaga acaagttcca ggagatctac tatcccaagg aacggaagaa
     5401 cgagctgtat atctacaaaa agaatctgtt cctgaacatc ggaaatccta actttgacaa
     5461 gatctacggc ctgattagca acgacatcaa gatggccgat gctaaattcc tgtttaatat
     5521 cgatggaaag aacatcagaa agaacaaaat cagtgagatc gacgctattc tgaagaatct
     5581 gaacgataaa ctgaacggct actcaaagga atacaaggag aagtacatca aaaagctgaa
     5641 ggagaacgac gatttctttg caaagaacat ccagaataag aactacaaat ccttcgaaaa
     5701 ggactataac cgcgtgtctg agtacaaaaa gattcgagat ctggtcgagt tcaactatct
     5761 gaacaaaatc gagtcctacc tgattgacat caactggaag ctggctattc agatggcaag
     5821 attcgaaagg gatatgcact atatcgtgaa tggactgagg gagctgggca tcattaagct
     5881 gtcaggctat aacaccggga tcagcagggc atacccaaag cgcaatggaa gcgacggctt
     5941 ttacactacc acagcctact acaagttctt tgatgaagag tcctacaaga agttcgagaa
     6001 gatttgctac gggtttggaa tcgacctgag cgaaaattcc gagatcaaca agcctgaaaa
     6061 tgagagcatt cggaactata tctcccattt ctacatcgtg agaaatccat ttgccgacta
     6121 cagtattgct gagcagatcg atcgggtgag caacctgctg tcatatagca cacgctacaa
     6181 caattcaact tatgccagcg tgttcgaagt ctttaaaaag gacgtgaatc tggactacga
     6241 tgagctgaaa aagaaattca aactgatcgg caacaatgat attctggagc gcctgatgaa
     6301 gcccaagaaa gtgagcgtcc tggaactgga gtcctacaac agtgactaca ttaagaatct
     6361 gatcattgaa ctgctgacca aaatcgagaa tactaacgat accctgTGAT TGACAGCTAG
     6421 CTCAGTCCTA GGTATAATGC TAGCCCACCC CAATATCGAA GGGGACTAAA ACGGAGACGG
     6481 AGATTCGTCT CGCCACCCCA ATATCGAAGG GGACTAAAAC aagctttaat gcggtagttt
     6541 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
     6601 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
     6661 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
     6721 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
     6781 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
     6841 gatcatggcg accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat
     6901 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     6961 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     7021 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     7081 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     7141 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     7201 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     7261 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     7321 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     7381 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     7441 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     7501 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     7561 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     7621 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     7681 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
     7741 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
     7801 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
     7861 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg
     7921 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
     7981 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
     8041 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
     8101 ttccgtgttt cgtaaagtct ggaaacgcgg aagtccccta cgtgctgctg aagttgcccg
     8161 caacagagag tggaaccaac cggtgatacc acgatactat gactgagagt caacgccatg
     8221 agcggcctca tttcttattc tgagttacaa cagtccgcac cgctgtccgg tagctccttc
     8281 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     8341 ctcgtaggac aggtgccggc agcgcccaac agtcccccgg ccacggggcc tgccaccata
     8401 cccacgccga aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     8461 tggctaccct gtggaaca
//