LOCUS       pR0035.2_pLac_C2C2.3    7783 bp ds-DNA     circular     13-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5699..5733
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5790..5825
                     /label="DR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    5883..7073
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5770..5789
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5734..5769
                     /label="DR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2240..5695
                     /label="LwC2c2-3"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG GtcttatccA TGAAAGTGAC CAAGGTCGAC GGCATCAGCC ACAAGAAGTA
     2281 CATCGAAGAG GGCAAGCTCG TGAAGTCCAC CAGCGAGGAA AACCGGACCA GCGAGAGACT
     2341 GAGCGAGCTG CTGAGCATCC GGCTGGACAT CTACATCAAG AACCCCGACA ACGCCTCCGA
     2401 GGAAGAGAAC CGGATCAGAA GAGAGAACCT GAAGAAGTTC TTTAGCAACA AGGTGCTGCA
     2461 CCTGAAGGAC AGCGTGCTGT ATCTGAAGAA CCGGAAAGAA AAGAACGCCG TGCAGGACAA
     2521 GAACTATAGC GAAGAGGACA TCAGCGAGTA CGACCTGAAA AACAAGAACA GCTTCTCCGT
     2581 GCTGAAGAAG ATCCTGCTGA ACGAGGACGT GAACTCTGAG GAACTGGAAA TCTTTCGGAA
     2641 GGACGTGGAA GCCAAGCTGA ACAAGATCAA CAGCCTGAAG TACAGCTTCG AAGAGAACAA
     2701 GGCCAACTAC CAGAAGATCA ACGAGAACAA CGTGGAAAAA GTGGGCGGCA AGAGCAAGCG
     2761 GAACATCATC TACGACTACT ACAGAGAGAG CGCCAAGCGC AACGACTACA TCAACAACGT
     2821 GCAGGAAGCC TTCGACAAGC TGTATAAGAA AGAGGATATC GAGAAACTGT TTTTCCTGAT
     2881 CGAGAACAGC AAGAAGCACG AGAAGTACAA GATCCGCGAG TACTATCACA AGATCATCGG
     2941 CCGGAAGAAC GACAAAGAGA ACTTCGCCAA GATTATCTAC GAAGAGATCC AGAACGTGAA
     3001 CAACATCAAA GAGCTGATTG AGAAGATCCC CGACATGTCT GAGCTGAAGA AAAGCCAGGT
     3061 GTTCTACAAG TACTACCTGG ACAAAGAGGA ACTGAACGAC AAGAATATTA AGTACGCCTT
     3121 CTGCCACTTC GTGGAAATCG AGATGTCCCA GCTGCTGAAA AACTACGTGT ACAAGCGGCT
     3181 GAGCAACATC AGCAACGATA AGATCAAGCG GATCTTCGAG TACCAGAATC TGAAAAAGCT
     3241 GATCGAAAAC AAACTGCTGA ACAAGCTGGA CACCTACGTG CGGAACTGCG GCAAGTACAA
     3301 CTACTATCTG CAAGTGGGCG AGATCGCCAC CTCCGACTTT ATCGCCCGGA ACCGGCAGAA
     3361 CGAGGCCTTC CTGAGAAACA TCATCGGCGT GTCCAGCGTG GCCTACTTCA GCCTGAGGAA
     3421 CATCCTGGAA ACCGAGAACG AGAACGGTAT CACCGGCCGG ATGCGGGGCA AGACCGTGAA
     3481 GAACAACAAG GGCGAAGAGA AATACGTGTC CGGCGAGGTG GACAAGATCT ACAATGAGAA
     3541 CAAGCAGAAC GAAGTGAAAG AAAATCTGAA GATGTTCTAC AGCTACGACT TCAACATGGA
     3601 CAACAAGAAC GAGATCGAGG ACTTCTTCGC CAACATCGAC GAGGCCATCA GCAGCATCAG
     3661 ACACGGCATC GTGCACTTCA ACCTGGAACT GGAAGGCAAG GACATCTTCG CCTTCAAGAA
     3721 TATCGCCCCC AGCGAGATCT CCAAGAAGAT GTTTCAGAAC GAAATCAACG AAAAGAAGCT
     3781 GAAGCTGAAA ATCTTCAAGC AGCTGAACAG CGCCAACGTG TTCAACTACT ACGAGAAGGA
     3841 TGTGATCATC AAGTACCTGA AGAATACCAA GTTCAACTTC GTGAACAAAA ACATCCCCTT
     3901 CGTGCCCAGC TTCACCAAGC TGTACAACAA GATTGAGGAC CTGCGGAATA CCCTGAAGTT
     3961 TTTTTGGAGC GTGCCCAAGG ACAAAGAAGA GAAGGACGCC CAGATCTACC TGCTGAAGAA
     4021 TATCTACTAC GGCGAGTTCC TGAACAAGTT CGTGAAAAAC TCCAAGGTGT TCTTTAAGAT
     4081 CACCAATGAA GTGATCAAGA TTAACAAGCA GCGGAACCAG AAAACCGGCC ACTACAAGTA
     4141 TCAGAAGTTC GAGAACATCG AGAAAACCGT GCCCGTGGAA TACCTGGCCA TCATCCAGAG
     4201 CAGAGAGATG ATCAACAACC AGGACAAAGA GGAAAAGAAT ACCTACATCG ACTTTATTCA
     4261 GCAGATTTTC CTGAAGGGCT TCATCGACTA CCTGAACAAG AACAATCTGA AGTATATCGA
     4321 GAGCAACAAC AACAATGACA ACAACGACAT CTTCTCCAAG ATCAAGATCA AAAAGGATAA
     4381 CAAAGAGAAG TACGACAAGA TCCTGAAGAA CTATGAGAAG CACAATCGGA ACAAAGAAAT
     4441 CCCTCACGAG ATCAATGAGT TCGTGCGCGA GATCAAGCTG GGGAAGATTC TGAAGTACAC
     4501 CGAGAATCTG AACATGTTTT ACCTGATCCT GAAGCTGCTG AACCACAAAG AGCTGACCAA
     4561 CCTGAAGGGC AGCCTGGAAA AGTACCAGTC CGCCAACAAA GAAGAAACCT TCAGCGACGA
     4621 GTTGGAACTG ATCAACCTGC TGAACCTGGA CAACAACAGA GTGACCGAGG ACTTCGAGCT
     4681 GGAAGCCAAC GAGATCGGCA AGTTCCTGGA CTTCAACGAA AACAAAATCA AGGACCGGAA
     4741 AGAGCTGAAA AAGTTCGACA CCAACAAGAT CTATTTCGAC GGCGAGAACA TCATCAAGCA
     4801 CCGGGCCTTC TACAATATCA AGAAATACGG CATGCTGAAT CTGCTGGAAA AGATCGCCGA
     4861 TAAGGCCAAG TATAAGATCA GCCTGAAAGA ACTGAAAGAG TACAGCAACA AGAAGAATGA
     4921 GATTGAAAAG AACTACACCA TGCAGCAGAA CCTGCACCGG AAGTACGCCA GACCCAAGAA
     4981 GGACGAAAAG TTCAACGACG AGGACTACAA AGAGTATGAG AAGGCCATCG GCAACATCCA
     5041 GAAGTACACC CACCTGAAGA ACAAGGTGGA ATTCAATGAG CTGAACCTGC TGCAGGGCCT
     5101 GCTGCTGAAG ATCCTGCACC GGCTCGTGGG CTACACCAGC ATCTGGGAGC GGGACCTGAG
     5161 ATTCCGGCTG AAGGGCGAGT TTCCCGAGAA CCACTACATC GAGGAAATTT TCAATTTCGA
     5221 CAACTCCAAG AATGTGAAGT ACAAAAGCGG CCAGATCGTG GAAAAGTATA TCAACTTCTA
     5281 CAAAGAACTG TACAAGGACA ATGTGGAAAA GCGGAGCATC TACTCCGACA AGAAAGTGAA
     5341 GAAACTGAAG CAGGAAAAAA AGGACCTGTA CATCCGGAAC TACATTGCCC ACTTCAACTA
     5401 CATCCCCCAC GCCGAGATTA GCCTGCTGGA AGTGCTGGAA AACCTGCGGA AGCTGCTGTC
     5461 CTACGACCGG AAGCTGAAGA ACGCCATCAT GAAGTCCATC GTGGACATTC TGAAAGAATA
     5521 CGGCTTCGTG GCCACCTTCA AGATCGGCGC TGACAAGAAG ATCGAAATCC AGACCCTGGA
     5581 ATCAGAGAAG ATCGTGCACC TGAAGAATCT GAAGAAAAAG AAACTGATGA CCGACCGGAA
     5641 CAGCGAGGAA CTGTGCGAAC TCGTGAAAGT CATGTTCGAG TACAAGGCCC TGGAATGATT
     5701 GACAGCTAGC TCAGTCCTAG GTATAATGCT AGCGATTTAG ACTACCCCAA AAACGAAGGG
     5761 GACTAAAACG GAGACGGAGA TTCGTCTCGG ATTTAGACTA CCCCAAAAAC GAAGGGGACT
     5821 AAAACaagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt
     5881 gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag
     5941 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca
     6001 gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg
     6061 cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc
     6121 tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatcctct
     6181 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     6241 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     6301 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     6361 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     6421 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag
     6481 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     6541 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
     6601 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
     6661 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
     6721 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
     6781 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
     6841 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
     6901 tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg
     6961 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
     7021 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
     7081 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
     7141 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
     7201 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
     7261 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
     7321 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
     7381 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
     7441 ccctacgtgc tgctgaagtt gcccgcaaca gagagtggaa ccaaccggtg ataccacgat
     7501 actatgactg agagtcaacg ccatgagcgg cctcatttct tattctgagt tacaacagtc
     7561 cgcaccgctg tccggtagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     7621 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc ccaacagtcc
     7681 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgccctgca ccattatgtt
     7741 ccggatctgc atcgcaggat gctgctggct accctgtgga aca
//