LOCUS       pR0035.3_pLac_C2C2.4    7687 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    3572..3574
                     /label="R449"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5603..5637
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5285..5287
                     /label="R1020"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5674..5693
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2240..5599
                     /label="Lse C2c2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5694..5729
                     /label="DR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5638..5673
                     /label="DR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5552..5599)
                     /label="end of protein"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    5787..6977
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgtggatcag cattaagacc ctgatccacc atctgggcgt
     2281 gctgttcttt tgcgattaca tgtataaccg gagagagaag aaaatcatcg aagtcaaaac
     2341 aatgaggatc actaaggtgg aggtcgatcg gaagaaagtg ctgatcagcc gggacaagaa
     2401 tggcgggaaa ctggtctatg agaacgaaat gcaggacaat acagagcaga tcatgcacca
     2461 taagaaaagc tccttctaca aaagcgtggt caacaagact atttgtcgcc ctgagcagaa
     2521 gcagatgaag aaactggtgc acggcctgct gcaggagaac agccaggaaa agatcaaggt
     2581 gagcgatgtc actaagctga atatctcaaa cttcctgaat cacagattca agaagagcct
     2641 gtactatttc ccagagaaca gtcccgataa gtcagaggaa tacagaatcg aaattaatct
     2701 gtctcagctg ctggaggaca gtctgaagaa acagcaggga accttcatct gctgggagtc
     2761 cttttctaag gacatggaac tgtatattaa ctgggccgag aactacatct ctagtaagac
     2821 taaactgatt aagaaaagta tcaggaacaa tcgcatccag tcaaccgaga gtaggtcagg
     2881 ccagctgatg gaccgctata tgaaggatat tctgaacaag aacaagccct tcgatatcca
     2941 gagcgtgtcc gagaaatacc agctggaaaa gctgacatcc gccctgaagg ctacttttaa
     3001 agaagctaag aaaaacgaca aggagatcaa ttacaagctg aaatctaccc tgcagaacca
     3061 cgagaggcag atcattgagg aactgaaaga gaatagcgaa ctgaaccagt tcaatatcga
     3121 gattcgcaag catctggaaa cttacttccc tattaagaaa accaacagaa aagtgggaga
     3181 tatcaggaat ctggagatcg gcgaaattca gaagatcgtg aaccaccgcc tgaagaacaa
     3241 gattgtccag cgaatcctgc aggagggaaa gctggcctcc tatgagatcg aatctacagt
     3301 gaactctaat agtctgcaga agatcaaaat tgaggaagcc ttcgctctga agtttatcaa
     3361 cgcttgcctg ttcgcatcta acaatctgag gaatatggtg taccccgtct gtaagaaaga
     3421 cattctgatg atcggcgagt tcaagaacag cttcaaggag attaagcaca agaagttcat
     3481 ccgccagtgg agccagttct tttcccagga aattactgtg gacgatatcg agctggcctc
     3541 ttggggactg cgaggagcaa ttgcccctat ccggaacgag atcattcacc tgaagaaaca
     3601 tagctggaag aaattcttta acaacccaac tttcaaagtg aagaaatcca agatcattaa
     3661 tggcaagacc aaagacgtga ccagcgagtt cctgtataag gaaaccctgt tcaaggatta
     3721 cttttattct gagctggaca gtgtgcccga actgatcatt aacaaaatgg agtcaagcaa
     3781 gatcctggac tactattcct ctgatcagct gaaccaggtg ttcaccattc ctaattttga
     3841 gctgtcactg ctgacaagcg ccgtgccctt cgctccttcc tttaaaagag tctatctgaa
     3901 gggcttcgac taccagaacc aggatgaggc acagccagac tataatctga agctgaacat
     3961 ctacaatgag aaagccttca acagcgaagc atttcaggcc cagtactccc tgtttaagat
     4021 ggtgtactat caggtgttcc tgccccagtt taccacaaac aatgatctgt tcaagagttc
     4081 agtggacttt atcctgaccc tgaacaaaga gaggaagggc tatgctaagg cattccagga
     4141 tatccgcaaa atgaataagg acgagaaacc ttccgaatac atgtcttata ttcagagtca
     4201 gctgatgctg taccagaaga aacaggagga aaaggagaaa atcaaccact tcgaaaagtt
     4261 tattaaccag gtgtttatca aagggttcaa cagctttatt gagaagaata ggctgaccta
     4321 catctgccac cctaccaaga acacagtgcc agagaacgat aatatcgaaa ttccattcca
     4381 tacagacatg gacgatagca atatcgcttt ttggctgatg tgcaaactgc tggatgcaaa
     4441 gcagctgtcc gagctgcgga acgaaatgat caagttcagc tgtagcctgc agagtacaga
     4501 ggaaatttca acttttacca aggcaagaga agtgatcggc ctggccctgc tgaacgggga
     4561 gaaaggatgt aatgactgga aggagctgtt cgacgataaa gaagcctgga agaaaaacat
     4621 gtcactgtat gtgagcgagg aactgctgca gtctctgccc tacacccagg aggatgggca
     4681 gacacctgtg attaatcgga gtatcgacct ggtcaagaaa tatggaacag agactatcct
     4741 ggaaaagctg ttcagctcct ctgacgatta taaggtgagc gcaaaagata ttgccaagct
     4801 gcacgagtac gacgtgacag aaaagattgc tcagcaggaa tccctgcata aacagtggat
     4861 cgagaagcca gggctggctc gggatagcgc atggactaag aaataccaga acgtgattaa
     4921 tgacatctcc aactaccagt gggcaaagac taaagtggag ctgacccagg tcagacacct
     4981 gcatcagctg accattgacc tgctgtcccg gctggccgga tacatgtcta tcgctgacag
     5041 agatttccag tttagttcaa actacattct ggagcgcgaa aatagcgaat accgagtgac
     5101 aagctggatt ctgctgtccg agaacaagaa caagaacaag tacaacgatt atgaactgta
     5161 taacctgaag aatgcctcca tcaaagtgag ctccaagaat gatccccagc tgaaagtcga
     5221 cctgaagcag ctgcggctga ccctggagta tctggaactg ttcgacaatc gactgaaaga
     5281 gaagcggaac aatatcagtc actttaacta cctgaatggc cagctgggga actcaattct
     5341 ggaactgttc gacgatgcca gagatgtgct gagctatgac aggaaactga agaatgctgt
     5401 ctccaagtct ctgaaagaaa tcctgtctag tcatggcatg gaggtgacct tcaagccact
     5461 gtaccagact aaccaccatc tgaagattga caaactgcag cccaagaaaa tccaccatct
     5521 gggggagaag tctacagtgt caagcaacca ggtcagtaac gaatactgtc agctggtgag
     5581 aactctgctg accatgaagT GATTGACAGC TAGCTCAGTC CTAGGTATAA TGCTAGCGTA
     5641 AGAGACTACC TCTATATGAA AGAGGACTAA AACGGAGACG GAGATTCGTC TCGGTAAGAG
     5701 ACTACCTCTA TATGAAAGAG GACTAAAACa agctttaatg cggtagttta tcacagttaa
     5761 attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg
     5821 gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg ccgggcctct
     5881 tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg ctagcgctat
     5941 atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac cgctttggcc
     6001 gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg atcatggcga
     6061 ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     6121 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     6181 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     6241 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     6301 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga
     6361 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     6421 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     6481 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     6541 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     6601 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     6661 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     6721 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     6781 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac
     6841 cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     6901 ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     6961 gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     7021 aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     7081 catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg
     7141 gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
     7201 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
     7261 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
     7321 gtaaagtctg gaaacgcgga agtcccctac gtgctgctga agttgcccgc aacagagagt
     7381 ggaaccaacc ggtgatacca cgatactatg actgagagtc aacgccatga gcggcctcat
     7441 ttcttattct gagttacaac agtccgcacc gctgtccggt agctccttcc ggtgggcgcg
     7501 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     7561 ggtgccggca gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     7621 acaagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg
     7681 tggaaca
//