LOCUS       pR0035.4_pLac_C2C2.5    8345 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    6333..6352
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(6221..6259)
                     /label="end of protein"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6353..6387
                     /label="DR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6263..6297
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2240..6259
                     /label="C2c2-5"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6298..6332
                     /label="DR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    6445..7635
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgcagatctc caaggtgaac cacaagcacg tggcagtggg
     2281 acagaaggac agggagagaa tcaccggctt catctataat gacccagtgg gcgatgagaa
     2341 gagcctggag gacgtggtgg caaagagggc caacgatacc aaggtgctgt tcaacgtgtt
     2401 caacacaaag gacctgtacg attctcagga gagcgacaag tccgagaagg ataaggagat
     2461 catctccaag ggcgccaagt tcgtggccaa gtcttttaac agcgccatca caatcctgaa
     2521 gaagcagaat aagatctatt ccaccctgac atctcagcag gtcatcaagg agctgaagga
     2581 caagttcggc ggcgcccgca tctacgacga tgacatcgag gaggccctga ccgagacact
     2641 gaagaagtct tttagaaagg agaatgtgag gaacagcatc aaggtgctga tcgagaatgc
     2701 agcaggcatc cggagctccc tgtccaagga cgaggaggag ctgatccagg agtatttcgt
     2761 gaagcagctg gtggaggagt acaccaagac aaagctgcag aagaacgtgg tgaagagcat
     2821 caagaatcag aacatggtca tccagcccga tagcgactcc caggtgctgt ctctgagcga
     2881 gtcccggaga gagaagcagt ctagcgccgt gtcctctgac accctggtga attgcaagga
     2941 gaaggacgtg ctgaaggcct tcctgacaga ttacgccgtg ctggatgagg acgagcgcaa
     3001 cagcctgctg tggaagctgc ggaatctggt gaacctgtac ttttatggct ctgagagcat
     3061 cagggattac tcttatacca aggagaagag cgtgtggaag gagcacgacg agcagaaggc
     3121 caacaagacc ctgttcatcg atgagatctg ccacatcaca aagatcggca agaacggcaa
     3181 ggagcagaag gtgctggact atgaggagaa taggagccgc tgtcggaagc agaatatcaa
     3241 ctactatcgc tccgccctga attacgccaa gaacaatacc agcggcatct tcgagaatga
     3301 ggattccaac cacttttgga ttcacctgat cgagaacgag gtggagcggc tgtataatgg
     3361 catcgagaac ggcgaggagt tcaagtttga gacaggctac atctctgaga aagtgtggaa
     3421 ggccgtgatc aaccacctga gcatcaagta catcgccctg ggcaaggccg tgtataacta
     3481 tgccatgaag gagctgagct cccccggcga catcgagcct ggcaagatcg atgactccta
     3541 tatcaacggc atcacctctt tcgactacga gatcatcaag gccgaggagt ccctgcagag
     3601 agatatctct atgaatgtgg tgtttgccac aaactacctg gcctgcgcca ccgtggatac
     3661 agacaaggat ttcctgctgt ttagcaagga ggacatcagg tcctgcacca agaaggatgg
     3721 caacctgtgc aagaacatca tgcagttctg gggcggctat tccacatgga agaatttttg
     3781 tgaggagtac ctgaaggatg acaaggacgc cctggagctg ctgtactccc tgaagtctat
     3841 gctgtattct atgagaaact ctagcttcca cttttctacc gagaatgtgg acaacggcag
     3901 ctgggataca gagctgatcg gcaagctgtt cgaggaggat tgtaaccgcg ccgcccggat
     3961 cgagaaggag aagttctaca acaataacct gcacatgttt tattcctcta gcctgctgga
     4021 gaaggtgctg gagagactgt actcctctca ccacgagagg gccagccagg tgccttcctt
     4081 caacagagtg tttgtgagga agaatttccc aagctccctg tccgagcaga gaatcacccc
     4141 caagtttaca gactccaagg atgagcagat ctggcagtct gccgtgtact atctgtgcaa
     4201 ggagatctac tataacgact tcctgcagag caaggaggcc tacaagctgt ttagggaggg
     4261 cgtgaagaat ctggacaaga acgatatcaa taaccagaag gccgccgata gcttcaagca
     4321 ggccgtggtg tactatggca aggccatcgg caacgccacc ctgtcccagg tgtgccaggc
     4381 catcatgaca gagtataata ggcagaataa cgacggcctg aagaagaaga gcgcctacgc
     4441 cgagaagcag aacagcaaca agtacaagca ctatcctctg ttcctgaagc aggtgctgca
     4501 gagcgccttt tgggagtacc tggatgagaa caaggagatc tatggcttca tctctgccca
     4561 gatccacaag agcaacgtgg agatcaaggc cgaggacttt atcgccaatt actctagcca
     4621 gcagtataag aagctggtgg ataaggtgaa gaaaacccct gagctgcaga agtggtatac
     4681 actgggccgc ctgatcaatc cacggcaggc caaccagttc ctgggctcca tcagaaatta
     4741 cgtgcagttt gtgaaggaca tccagaggcg cgccaaggag aatggcaacc caatcaggaa
     4801 ctactatgag gtgctggagt ccgattctat catcaagatc ctggagatgt gcaccaagct
     4861 gaatggcacc acaagcaacg acatccacga ttacttccgc gacgaggatg agtacgccga
     4921 gtatatctcc cagttcgtga actttggcga cgtgcactct ggcgccgccc tgaatgcctt
     4981 ttgcaacagc gagtccgagg gcaagaagaa cggcatctac tatgacggca tcaatcccat
     5041 cgtgaatcgg aactgggtgc tgtgcaagct gtatggctcc cctgatctga tctctaagat
     5101 catcagccgc gtgaatgaga acatgatcca cgacttccac aagcaggagg atctgatccg
     5161 ggagtaccag atcaagggca tctgttctaa caagaaggag cagcaggacc tgcgcacctt
     5221 tcaggtgctg aagaatcgcg tggagctgcg ggatatcgtg gagtacagcg agatcatcaa
     5281 cgagctgtat ggccagctga tcaagtggtg ctacctgaga gagagggacc tgatgtactt
     5341 ccagctgggc tttcactacc tgtgcctgaa taacgcctcc tctaaggagg ccgattatat
     5401 caagatcaat gtggatgacc ggaacatcag cggcgccatc ctgtaccaga tcgccgccat
     5461 gtatatcaat ggcctgcccg tgtactataa gaaggatgac atgtacgtgg ccctgaagtc
     5521 cggcaagaag gcctctgacg agctgaatag caacgagcag acctccaaga agatcaacta
     5581 cttcctgaag tatggcaata acatcctggg cgacaagaag gatcagctgt acctggccgg
     5641 cctggagctg ttcgagaatg tggccgagca cgagaacatc atcatcttta gaaatgagat
     5701 cgaccacttc cactactttt atgaccgcga tcggtccatg ctggatctgt attctgaggt
     5761 gtttgaccgc ttctttacct acgatatgaa gctgcggaag aatgtggtga acatgctgta
     5821 taacatcctg ctggaccaca atatcgtgag ctccttcgtg tttgagacag gcgagaagaa
     5881 ggtcggcagg ggcgatagcg aagtgatcaa gccttccgcc aagatcagac tgagggccaa
     5941 taacggcgtg tctagcgacg tgttcaccta caaagtgggc agcaaggatg agctgaagat
     6001 cgccacactg ccagccaaga acgaggagtt tctgctgaat gtggccagac tgatctacta
     6061 tcccgacatg gaggccgtgt ccgagaacat ggtgagggag ggcgtggtga aggtggagaa
     6121 gtctaatgat aagaagggca agatcagcag aggctccaat accaggtcct ctaaccagtc
     6181 taagtacaac aacaagagca agaacagaat gaactactct atgggcagca tcttcgagaa
     6241 gatggacctg aagtttgatT GATTGACAGC TAGCTCAGTC CTAGGTATAA TGCTAGCGTA
     6301 TTGAGAAAAG CCAGATATAG TTGGCAATAG ACGGAGACGG AGATTCGTCT CGGTATTGAG
     6361 AAAAGCCAGA TATAGTTGGC AATAGACaag ctttaatgcg gtagtttatc acagttaaat
     6421 tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
     6481 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
     6541 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat
     6601 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc
     6661 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc
     6721 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     6781 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     6841 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     6901 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     6961 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     7021 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     7081 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     7141 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     7201 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     7261 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     7321 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     7381 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     7441 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg
     7501 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     7561 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     7621 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     7681 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     7741 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt
     7801 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     7861 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     7921 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     7981 aaagtctgga aacgcggaag tcccctacgt gctgctgaag ttgcccgcaa cagagagtgg
     8041 aaccaaccgg tgataccacg atactatgac tgagagtcaa cgccatgagc ggcctcattt
     8101 cttattctga gttacaacag tccgcaccgc tgtccggtag ctccttccgg tgggcgcggg
     8161 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     8221 tgccggcagc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     8281 aagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
     8341 gaaca
//