LOCUS       pR0035.7_pLac_C2C2.8    7678 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5685..5720
                     /label="DR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    5444..5446
                     /label="R1069"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5629..5664
                     /label="DR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2240..5590
                     /label="Cg C2c2-8"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5778..6968
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3638..3640
                     /label="R467"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5594..5628
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5665..5684
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgcggatcac aaaggtgaag atcaagctgg acaacaagct
     2281 gtaccaggtg accatgcaga aggaggagaa gtatggcaca ctgaagctga atgaggagag
     2341 caggaagtcc accgccgaga tcctgcgcct gaagaaggcc agcttcaaca agtcttttca
     2401 cagcaagacc atcaattccc agaaggagaa caagaatgcc acaatcaaga agaacggcga
     2461 ctacatcagc cagatcttcg agaagctggt gggcgtggat accaacaaga atatcaggaa
     2521 gcccaagatg tccctgacag acctgaagga tctgcctaag aaggacctgg ccctgttcat
     2581 caagcgcaag tttaagaacg acgatatcgt ggagatcaag aatctggatc tgatctccct
     2641 gttttataat gccctgcaga aggtgccagg cgagcacttc accgacgagt cttgggccga
     2701 tttttgccag gagatgatgc cctaccggga gtataagaac aagttcatcg agagaaagat
     2761 catcctgctg gccaactcca tcgagcagaa taagggcttc tctatcaatc ccgagacatt
     2821 cagcaagcgg aagagagtgc tgcaccagtg ggcaatcgag gtgcaggaga ggggcgactt
     2881 ttccatcctg gatgagaagc tgtctaagct ggccgagatc tacaacttca agaagatgtg
     2941 caagagagtg caggacgagc tgaacgatct ggagaagagc atgaagaagg gcaagaatcc
     3001 tgagaaggag aaggaggcct ataagaagca gaagaacttt aagatcaaga ccatctggaa
     3061 ggactaccca tataagacac acatcggcct gatcgagaag atcaaggaga atgaggagct
     3121 gaaccagttc aatatcgaga tcggcaagta cttcgagcac tattttccaa tcaagaagga
     3181 gagatgcacc gaggatgagc cctactatct gaacagcgag acaatcgcca ccacagtgaa
     3241 ctaccagctg aagaatgccc tgatctccta cctgatgcag atcggcaagt ataagcagtt
     3301 tggcctggag aatcaggtgc tggactccaa gaagctgcag gagatcggca tctatgaggg
     3361 cttccagacc aagtttatgg atgcctgcgt gttcgccaca agctccctga agaacatcat
     3421 cgagcctatg cggagcggcg acatcctggg caagagagag tttaaggagg ccatcgccac
     3481 atctagcttc gtgaattacc accacttctt tccctatttc ccttttgagc tgaagggcat
     3541 gaaggatagg gagtccgagc tgatcccatt tggcgagcag accgaggcca agcagatgca
     3601 gaacatctgg gccctgaggg gctctgtgca gcagatccgc aatgagatct tccacagctt
     3661 tgacaagaac cagaagttca atctgcccca gctggacaag agcaacttcg agtttgatgc
     3721 ctccgagaac agcaccggca agtctcagag ctacatcgag acagattata agttcctgtt
     3781 tgaggccgag aagaaccagc tggagcagtt ctttatcgag cgcatcaagt cctctggcgc
     3841 cctggagtac tatcccctga agtccctgga gaagctgttc gccaagaagg agatgaagtt
     3901 tagcctgggc tcccaggtgg tggccttcgc ccctagctac aagaagctgg tgaagaaggg
     3961 ccactcctat cagaccgcca cagagggcac cgccaactac ctgggcctgt cctactataa
     4021 taggtatgag ctgaaggagg agtcttttca ggcccagtac tatctgctga agctgatcta
     4081 ccagtacgtg ttcctgccta acttttccca gggcaattct ccagccttcc gcgagacagt
     4141 gaaggccatc ctgcggatca acaaggacga ggccagaaag aagatgaaga agaataagaa
     4201 gttcctgagg aagtacgcct ttgagcaggt gcgcgagatg gagttcaagg agacacccga
     4261 ccagtacatg agctatctgc agtccgagat gagggaggag aaggtgcgca aggccgagaa
     4321 gaacgataag ggcttcgaga agaacatcac catgaatttt gagaagctgc tgatgcagat
     4381 cttcgtgaag ggcttcgacg tgttcctgac cacatttgcc ggcaaggagc tgctgctgag
     4441 ctccgaggag aaagtgatca aggagacaga gatctccctg tctaagaaga tcaacgagcg
     4501 ggagaaaacc ctgaaggcca gcatccaggt ggagcaccag ctggtggcca ccaattctgc
     4561 catcagctac tggctgttct gcaagctgct ggactcccgg cacctgaacg agctgagaaa
     4621 tgagatgatc aagttcaagc agagccggat caagttcaac cacacacagc acgccgagct
     4681 gatccagaat ctgctgccta tcgtggagct gaccatcctg tctaacgact acgatgagaa
     4741 gaacgactcc cagaatgtgg acgtgagcgc ctattttgag gataagagcc tgtacgagac
     4801 agccccttat gtgcagaccg acgataggac acgcgtgtct ttcaggccaa tcctgaagct
     4861 ggagaagtac cacacaaaga gcctgatcga ggccctgctg aaggacaacc cacagtttcg
     4921 cgtggccgcc accgatatcc aggagtggat gcacaagagg gaggagatcg gcgagctggt
     4981 ggagaagcgc aagaatctgc acaccgagtg ggcagaggga cagcagacac tgggagcaga
     5041 gaagcgggag gagtacagag actattgtaa gaagatcgat cggttcaact ggaaggccaa
     5101 taaggtgacc ctgacatacc tgtctcagct gcactatctg atcacagacc tgctgggcag
     5161 aatggtgggc ttcagcgccc tgtttgagag ggatctggtg tacttcagcc gctccttttc
     5221 tgagctgggc ggcgagacat accacatcag cgattataag aacctgtccg gcgtgctgcg
     5281 gctgaatgcc gaggtgaagc ccatcaagat caagaacatc aaagtgatcg acaacgagga
     5341 gaatccttac aagggcaatg agccagaggt gaagcccttc ctggatcggc tgcacgccta
     5401 tctggagaac gtgatcggca tcaaggccgt gcacggcaag atcagaaatc agacagccca
     5461 cctgtccgtg ctgcagctgg agctgtctat gatcgagagc atgaacaatc tgcgggacct
     5521 gatggcctac gatagaaagc tgaagaacgc cgtgaccaag tctatgatca agatcctgga
     5581 caagcacggc TGATTGACAG CTAGCTCAGT CCTAGGTATA ATGCTAGCAT TAAAGACTAC
     5641 CTCTAAATGT AAGAGGACTA TAACGGAGAC GGAGATTCGT CTCGATTAAA GACTACCTCT
     5701 AAATGTAAGA GGACTATAAC aagctttaat gcggtagttt atcacagtta aattgctaac
     5761 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
     5821 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
     5881 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
     5941 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
     6001 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
     6061 tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     6121 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     6181 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     6241 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     6301 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga
     6361 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     6421 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     6481 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     6541 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     6601 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     6661 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     6721 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     6781 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
     6841 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     6901 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     6961 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc
     7021 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat
     7081 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc
     7141 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
     7201 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     7261 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     7321 ggaaacgcgg aagtccccta cgtgctgctg aagttgcccg caacagagag tggaaccaac
     7381 cggtgatacc acgatactat gactgagagt caacgccatg agcggcctca tttcttattc
     7441 tgagttacaa cagtccgcac cgctgtccgg tagctccttc cggtgggcgc ggggcatgac
     7501 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     7561 agcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgcc
     7621 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaaca
//