LOCUS       pR0035.8_pLac_C2C2.9    7993 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    6000..6035
                     /label="DR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    5909..5943
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    6093..7283
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3782..3784
                     /label="R515"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2240..5905
                     /label="Cg2 C2c2-9"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5980..5999
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5944..5979
                     /label="DR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5579..5581
                     /label="R1114"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgatcctgaa gctgaagatc gacgagaacc acaagaattt
     2281 cgagatcgag agcctgatcc caaaggagat catccacctg aaggataagg ccatcaagac
     2341 caaccaggtg tccgaggagt actgccagct ggtgctggcc ctgctgacca caaaccccgg
     2401 caatcagctg aacatgagga tgacaaaggt gaagatcaac ggcagccccg tgagcatgaa
     2461 tcgcagcaag ctgaacggcc acctggtgtg gaatggcacc acaaataccg tgaacatcct
     2521 gacaaagaag gagcagagct tcgccgcctc ctttctgaac aagaccctgg tgaaggccga
     2581 ccaggtgaag ggctacaagg tgctggccga gaatatcttc atcatctttg agcagctgga
     2641 gaagtccaac tctgagaagc ccagcgtgta cctgaacaat atccggagac tgaaggaggc
     2701 cggcctgaag cggttcttta agagcaagta ccacgaggag atcaagtata cctctgagaa
     2761 gaatcagagc gtgccaacaa agctgaatct gatccccctg ttctttaacg ccgtggacag
     2821 aatccaggag gacaagttcg atgagaagaa ctggtcctac ttttgcaagg agatgtctcc
     2881 ctacctggat tataagaaga gctatctgaa taggaagaag gagatcctgg ccaattctat
     2941 ccagcagaac aggggcttca gcatgccaac cgcagaggag cctaacctgc tgtctaagcg
     3001 gaagcagctg ttccagcagt gggccatgaa gtttcaggag agccctctga tccagcagaa
     3061 caattttgcc gtggagcagt tcaataagga gtttgccaat aagatcaacg agctggccgc
     3121 cgtgtataac gtggacgagc tgtgcaccgc catcacagag aagctgatga acttcgacaa
     3181 ggataagtcc aataagacca gaaactttga gatcaagaag ctgtggaagc agcaccctca
     3241 caacaaggat aaggccctga tcaagctgtt caatcaggag ggcaacgagg ccctgaatca
     3301 gtttaacatc gagctgggca agtacttcga gcactatttt cctaagacag gcaagaagga
     3361 gagcgccgag tcctactatc tgaatccaca gaccatcatc aagacagtgg gctaccagct
     3421 gaggaacgcc ttcgtgcagt atctgctgca agtgggcaag ctgcaccagt acaacaaggg
     3481 cgtgctggac agccagaccc tgcaggagat cggcatgtat gagggcttcc agacaaagtt
     3541 tatggatgcc tgcgtgttcg ccagctcctc tctgcggaat atcatccagg ccaccacaaa
     3601 cgaggacatc ctgaccagag agaagtttaa gaaggagctg gagaagaacg tggagctgaa
     3661 gcacgacctg ttctttaaga ccgagatcgt ggaggagcgc gatgagaatc ctgccaagaa
     3721 gatcgccatg acaccaaacg agctggacct gtgggcaatc aggggagccg tgcagagggt
     3781 gcgcaatcag atcttccacc agcagatcaa taagagacac gagcccaacc agctgaaggt
     3841 cggctccttt gagaatggcg atctgggcaa cgtgtcttac cagaaaacca tctatcagaa
     3901 gctgttcgac gccgagatca aggatatcga gatctacttt gccgagaaga tcaagagctc
     3961 cggcgccctg gagcagtatt ccatgaagga cctggagaag ctgttcagca acaaggagct
     4021 gacactgtcc ctgggaggac aggtggtggc cttcgcccct tcttacaaga agctgtataa
     4081 gcagggctac ttttatcaga atgagaaaac catcgagctg gagcagttta cagactacga
     4141 tttctctaat gacgtgttca aggccaacta ctatctgatc aagctgatct accactacgt
     4201 gttcctgcca cagtttagcc aggccaacaa taagctgttc aaggacaccg tgcactacgt
     4261 gatccagcag aataaggagc tgaacaccac agagaaggat aagaagaaca ataagaagat
     4321 cagaaagtat gcctttgagc aggtgaagct gatgaagaac gagtccccag agaagtacat
     4381 gcagtatctg cagagggaga tgcaggagga gcgcaccatc aaggaggcca agaaaaccaa
     4441 cgaggagaag cccaactaca atttcgagaa gctgctgatc cagatcttta tcaagggctt
     4501 cgacaccttt ctgaggaatt tcgatctgaa cctgaatcct gccgaggagc tggtgggcac
     4561 agtgaaggag aaggccgagg gcctgcggaa gagaaaggag cgcatcgcca agatcctgaa
     4621 cgtggacgag cagatcaaga ccggcgatga ggagatcgcc ttctggatct ttgcaaagct
     4681 gctggacgca aggcacctga gcgagctgag aaacgagatg atcaagttta agcagtctag
     4741 cgtgaagaag ggcctgatca agaatggcga tctgatcgag cagatgcagc caatcctgga
     4801 gctgtgcatc ctgagcaacg acagcgagtc catggagaag gagtccttcg ataagatcga
     4861 ggtgtttctg gagaaggtgg agctggccaa gaatgagcca tacatgcagg aggacaagct
     4921 gacccccgtg aagttcaggt ttatgaagca gctggagaag tatcagacac gcaatttcat
     4981 cgagaacctg gtcatcgaga atccagagtt taaggtgtcc gagaagatcg tgctgaactg
     5041 gcacgaggag aaggagaaga tcgccgacct ggtggataag cggaccaagc tgcacgagga
     5101 gtgggcctcc aaggccagag agatcgagga gtacaatgag aagatcaaga agaacaagtc
     5161 taagaagctg gacaagcccg ccgagttcgc caagtttgcc gagtataaga tcatctgtga
     5221 ggccatcgag aacttcaata ggctggatca caaggtgcgc ctgacatacc tgaagaacct
     5281 gcactatctg atgatcgacc tgatgggcag gatggtgggc ttctccgtgc tgtttgagcg
     5341 ggatttcgtg tatatgggca gatcttatag cgccctgaag aagcagtcta tctacctgaa
     5401 tgactatgat accttcgcca acatccgcga ctgggaggtg aacgagaata agcacctgtt
     5461 tggcacatcc tctagcgatc tgaccttcca ggagacagcc gagtttaaga atctgaagaa
     5521 gcccatggag aaccagctga aggccctgct gggcgtgacc aaccacagct tcgagatccg
     5581 gaacaatatc gcccacctgc acgtgctgag aaatgatggc aagggcgagg gcgtgtctct
     5641 gctgagctgc atgaacgacc tgaggaagct gatgtcctac gatcgcaagc tgaagaatgc
     5701 cgtgacaaag gccatcatca agatcctgga caagcacggc atgatcctga agctgaccaa
     5761 caatgatcac acaaagccct tcgagattga gagcctgaag cctaagaaga tcatccacct
     5821 ggagaagtcc aaccactctt tccctatgga ccaggtgtcc caggagtact gtgatctggt
     5881 gaagaagatg ctggtgttta ccaatTGATT GACAGCTAGC TCAGTCCTAG GTATAATGCT
     5941 AGCAATATAA ACTACCTCTA AATGTAAGAG GACTATAACG GAGACGGAGA TTCGTCTCGA
     6001 ATATAAACTA CCTCTAAATG TAAGAGGACT ATAACaagct ttaatgcggt agtttatcac
     6061 agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
     6121 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
     6181 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
     6241 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
     6301 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
     6361 tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
     6421 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     6481 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     6541 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     6601 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     6661 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     6721 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     6781 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     6841 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     6901 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     6961 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     7021 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     7081 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     7141 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     7201 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     7261 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     7321 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     7381 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     7441 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     7501 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     7561 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     7621 tgtttcgtaa agtctggaaa cgcggaagtc ccctacgtgc tgctgaagtt gcccgcaaca
     7681 gagagtggaa ccaaccggtg ataccacgat actatgactg agagtcaacg ccatgagcgg
     7741 cctcatttct tattctgagt tacaacagtc cgcaccgctg tccggtagct ccttccggtg
     7801 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     7861 aggacaggtg ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     7921 gccgaaacaa gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
     7981 accctgtgga aca
//