LOCUS       pR0035.11_pLac_C2C2.12  7479 bp ds-DNA     circular     13-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    5487..5522
                     /label="DR (note missing a T)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5431..5466
                     /label="DR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2240..5392
                     /label="C2c2-12"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5467..5486
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    5396..5430
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5579..6769
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgaagatcac caagatgcgg gtggacggca gaaccatcgt
     2281 gatggagcgg acatccaagg agggccagct gggctatgag ggcatcgacg gcaacaagac
     2341 cacagagatc atctttgata agaagaagga gagcttctac aagtccatcc tgaacaagac
     2401 cgtgagaaag cctgatgaga aggagaagaa tcggagaaag caggccatca acaaggccat
     2461 caataaggag atcacagagc tgatgctggc cgtgctgcac caggaggtgc ctagccagaa
     2521 gctgcacaac ctgaagagcc tgaataccga gtccctgaca aagctgttta agccaaagtt
     2581 ccagaatatg atctcttatc cacctagcaa gggagcagag cacgtgcagt tctgcctgac
     2641 cgacatcgcc gtgcccgcaa tccgggacct ggatgagatc aagcctgact ggggcatctt
     2701 ctttgagaag ctgaagccat acacagattg ggccgagtcc tacatccact ataagcagac
     2761 cacaatccag aagtctatcg agcagaacaa gatccagagc ccagactccc ccagaaagct
     2821 ggtgctgcag aagtatgtga ccgcctttct gaatggagag ccactgggcc tggacctggt
     2881 ggccaagaag tacaagctgg ccgatctggc cgagtctttc aagctggtgg acctgaacga
     2941 ggataagagc gccaattata agatcaaggc ctgtctgcag cagcaccagc ggaacatcct
     3001 ggacgagctg aaggaggacc ccgagctgaa tcagtacggc atcgaggtga agaagtacat
     3061 ccagaggtat ttcccaatca agagggcccc caaccgctct aagcacgcca gagccgactt
     3121 tctgaagaag gagctgatcg agagcaccgt ggagcagcag ttcaagaatg ccgtgtacca
     3181 ctatgtgctg gagcagggca agatggaggc ctacgagctg accgatccca agacaaagga
     3241 cctgcaggat atcaggtctg gcgaggcctt cagctttaag ttcatcaacg cctgcgcctt
     3301 tgcctccaac aatctgaaga tgatcctgaa ccctgagtgt gagaaggaca tcctgggcaa
     3361 gggcaatttc aagaagaacc tgccaaattc caccacacgc tctgatgtgg tgaagaagat
     3421 gatccccttc tttagcgacg agctgcagaa cgtgaatttc gatgaggcca tctgggccat
     3481 ccggggctcc atccagcaga tcagaaatga ggtgtatcac tgcaagaagc actcttggaa
     3541 gagcatcctg aagatcaagg gctttgagtt cgagccaaac aatatgaagt acgccgacag
     3601 cgatatgcag aagctgatgg acaaggatat cgccaagatc cccgagttta tcgaggagaa
     3661 gctgaagagc tccggcgtgg tgcggttcta cagacacgac gagctgcaga gcatctggga
     3721 gatgaagcag ggcttttccc tgctgaccac aaacgcccct tttgtgccca gcttcaagcg
     3781 ggtgtacgcc aagggccacg actatcagac ctccaagaac agatactata atctggacct
     3841 gaccacattc gatatcctgg agtacggcga ggaggatttt cgggccagat atttcctgac
     3901 caagctggtg tactatcagc agtttatgcc ctggttcaca gccgacaaca atgcctttag
     3961 ggatgccgcc aacttcgtgc tgaggctgaa caagaatcgc cagcaggacg ccaaggcctt
     4021 tatcaatatc cgggaggtgg aggagggcga gatgcctaga gattacatgg gctatgtgca
     4081 gggccagatc gccatccacg aggacagcat cgaggatacc ccaaaccact ttgagaagtt
     4141 catctcccag gtgtttatca agggcttcga caggcacatg cgctctgcca atctgaagtt
     4201 catcaagaac ccccgcaatc agggcctgga gcagtccgag atcgaggaga tgtcttttga
     4261 tatcaaggtg gagccttctt tcctgaagaa caaggacgat tatatcgcct tttggatctt
     4321 ctgcaagatg ctggacgcaa ggcacctgag cgagctgaga aatgagatga tcaagtacga
     4381 tggccacctg accggcgagc aggagatcat cggcctggcc ctgctgggag tggactcccg
     4441 ggagaacgat tggaagcagt tcttttctag cgagagagag tacgagaaga tcatgaaggg
     4501 ctatgtggtg gaggagctgt accagcggga gccttataga cagtctgacg gcaagacacc
     4561 aatcctgttc aggggagtgg agcaggcaag gaagtacggc accgagacag tgatccagag
     4621 gctgtttgat gccaaccccg agttcaaggt gagcaagtgt aatctggccg agtgggagcg
     4681 ccagaaggag acaatcgagg agacaatcaa gaggcgcaag gagctgcaca acgagtgggc
     4741 caagaatccc aagaagcctc agaacaatgc cttctttaag gagtacaagg agtgctgtga
     4801 cgccatcgat gcctataact ggcacaagaa taagaccaca ctggcctacg tgaacgagct
     4861 gcaccacctg ctgatcgaga tcctgggcag gtacgtgggc tatgtggcca tcgccgaccg
     4921 cgattttcag tgcatggcca accagtattt caagcactcc ggcatcaccg agagggtgga
     4981 gtactggggc gacaatcgcc tgaagtctat caagaagctg gatacatttc tgaagaagga
     5041 gggcctgttc gtgagcgaga agaacgcccg gaatcacatc gcccacctga actacctgtc
     5101 cctgaagtct gagtgtaccc tgctgtatct gtccgagagg ctgcgcgaga tctttaagta
     5161 cgaccggaag ctgaagaatg ccgtgtccaa gtctctgatc gacatcctgg atagacacgg
     5221 catgtccgtg gtgttcgcca acctgaagga gaataagcac cggctggtca tcaagagcct
     5281 ggagcctaag aagctgcgcc acctgggcgg caagaagatc gatggcggct acatcgagac
     5341 aaaccaggtg agcgaggagt attgtggcat cgtgaagaga ctgctggaga tgTGATTGAC
     5401 AGCTAGCTCA GTCCTAGGTA TAATGCTAGC GATTTAGAGT ACCTCAAAAC AAAAGAGGAC
     5461 TAAAACGGAG ACGGAGATTC GTCTCGGATT TAGAGACCTC AAAACAAAAG AGGACTAAAA
     5521 Caagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat
     5581 gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat
     5641 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat
     5701 cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc
     5761 cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact
     5821 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga tcctctacgc
     5881 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     5941 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     6001 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     6061 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     6121 gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag
     6181 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     6241 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt
     6301 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
     6361 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat
     6421 cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg
     6481 gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca
     6541 ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc
     6601 ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga tttatgccgc
     6661 ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg
     6721 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg
     6781 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
     6841 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     6901 agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg
     6961 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
     7021 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
     7081 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcccct
     7141 acgtgctgct gaagttgccc gcaacagaga gtggaaccaa ccggtgatac cacgatacta
     7201 tgactgagag tcaacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca
     7261 ccgctgtccg gtagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     7321 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgcccaa cagtcccccg
     7381 gccacggggc ctgccaccat acccacgccg aaacaagcgc cctgcaccat tatgttccgg
     7441 atctgcatcg caggatgctg ctggctaccc tgtggaaca
//