LOCUS       pR0035.13_pLac_C2C2.14  8182 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2240..6094
                     /label="Rc C2c2-14"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    5393..5395
                     /label="R1052"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6189..6224
                     /label="DR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6133..6168
                     /label="DR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6169..6188
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6282..7472
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3629..3631
                     /label="R464"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6098..6132
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgcagattgg aaaagtgcag ggaaggacta ttagcgagtt
     2281 tggggacccc gcaggaggac tgaagagaaa gattagcacc gatggaaaga atcgcaagga
     2341 actgccagca cacctgtcaa gtgatccaaa ggccctgatt ggacagtgga tctcagggat
     2401 cgacaaaatc taccggaagc ccgatagtag gaaatccgat ggcaaagcaa tccactcccc
     2461 aacccctagc aaaatgcagt ttgacgcaag ggatgacctg ggagaggcat tctggaaact
     2521 ggtgtcagaa gctggcctgg ctcaggatag tgactatgat cagtttaagc ggcgactgca
     2581 tccatacgga gacaaatttc agcctgctga tagcggagca aaactgaagt tcgaagccga
     2641 cccacctgag cctcaggcct tccacggcag gtggtatgga gcaatgtcca agcgcgggaa
     2701 tgacgcaaag gagctggcag ccgctctgta cgaacatctg cacgtcgatg agaaaagaat
     2761 cgatggccag cctaagcgga atcctaagac tgataaattc gctccagggc tggtggtggc
     2821 ccgcgctctg gggattgagt caagcgtgct gccaagagga atggctagac tggctaggaa
     2881 ttggggcgaa gaggaaatcc agacttactt tgtcgtggat gtggccgcat cagtgaagga
     2941 ggtggctaaa gctgctgtga gcgcagcaca ggctttcgat ccacctcgcc aggtcagcgg
     3001 gagaagtctg agtcccaaag tggggttcgc actggcagag catctggaaa gggtgacagg
     3061 ctccaagagg tgctcattcg atcccgcagc cggcccttct gtcctggcac tgcacgacga
     3121 agtgaagaaa acttataaaa gactgtgcgc ccggggaaag aatgcagcca gagcttttcc
     3181 agccgacaag accgaactgc tggctctgat gagacatacc catgagaaca gagtccggaa
     3241 tcagatggtc agaatgggaa gggtgtcaga ataccgaggc cagcaggccg gggatctggc
     3301 tcagtcccac tattggacaa gtgccggcca gacagaaatc aaagaaagcg agatctttgt
     3361 caggctgtgg gtgggagctt ttgcactggc tgggcgcagc atgaaagcat ggattgaccc
     3421 aatggggaag atcgtgaaca ctgagaagaa tgatagagat ctgactgccg ccgtcaacat
     3481 cagacaggta atctccaaca aggagatggt cgctgaggca atggctcggc gcggaatcta
     3541 tttcggcgag acaccagagc tggatagact gggggcagag ggaaatgaag gcttcgtgtt
     3601 cgctctgctg cgatacctga gagggtgtag aaaccagact tttcatctgg gggctcgggc
     3661 aggatttctg aaggaaatcc gcaaggagct ggaaaagaca cggtggggca aggcaaaaga
     3721 agccgagcat gtggtcctga ccgacaagac agtggcagcc attagagcaa tcattgacaa
     3781 tgacgccaaa gccctgggcg cccggctgct ggcagacctg agcggagctt ttgtggcaca
     3841 ctatgcaagc aaagaacact tcagcacact gtattccgag atcgtcaaag cagtgaaaga
     3901 cgctcctgaa gtgtcatccg ggctgcctcg gctgaagctg ctgctgaaac gggcagacgg
     3961 agtcagggga tacgtgcatg gactgcggga tacccgaaaa catgctttcg ccactaagct
     4021 gccacctcct ccagctccac gggaactgga cgaccctgca acaaaggcca ggtatatcgc
     4081 tctgctgcgg ctgtatgatg gcccatttcg ggcctacgca agtggaatca ctggaactgc
     4141 cctggcagga cctgctgcca gagctaaaga ggcagctact gcactggccc agtccgtgaa
     4201 tgtcactaaa gcatattccg atgtgatgga gggacgcacc tcacgactga ggccacctaa
     4261 tgatggcgaa accctgaggg aatacctgag tgcactgact ggcgagactg ccacagagtt
     4321 ccgggtgcag attggctatg agtccgactc agagaatgct agaaagcagg ccgagtttat
     4381 cgagaactat aggcgagata tgctggcctt catgttcgag gattacattc gagcaaaggg
     4441 gttcgactgg attctgaaga ttgaacctgg ggccacagca atgaccagag cccctgtgct
     4501 gccagagcca atcgatacac gaggccagta tgaacactgg caggccgcac tgtacctggt
     4561 catgcacttc gtcccagcta gtgatgtgag caacctgctg caccagctga gaaagtggga
     4621 agcactgcag ggcaaatacg agctggtcca ggatggggac gccacagacc aggcagatgc
     4681 ccggagggag gccctggatc tggtgaaaag gttccgcgat gtgctggtcc tgtttctgaa
     4741 aacaggggaa gccagatttg aaggccgcgc cgctccattt gatctgaagc ctttccgggc
     4801 actgtttgct aatccagcta ctttcgatcg gctgtttatg gccacaccaa ccactgctag
     4861 gcccgctgaa gatgaccccg agggcgacgg agcctccgaa ccagagctgc gggtcgccag
     4921 gacactgcgc gggctgcgcc agattgcacg atataaccac atggcagtgc tgagcgatct
     4981 gttcgctaaa cataaagtgc gggatgagga agtcgcacgc ctggccgaga ttgaggacga
     5041 aactcaggaa aaatctcaga ttgtcgccgc acaggaactg aggacagatc tgcacgataa
     5101 agtcatgaaa tgccacccta aaaccatcag ccccgaggag cggcagagtt acgccgccgc
     5161 aatcaaaacc attgaggagc acaggttcct ggtgggcaga gtgtacctgg gggatcacct
     5221 gcggctgcat cgcctgatga tggatgtcat cggccgcctg attgattacg ctggcgctta
     5281 tgagcgcgat actggcactt tcctgatcaa cgccagcaaa cagctggggg ctggcgctga
     5341 ttgggcagtg accattgcag gcgccgctaa taccgacgca cgcacccaga cccggaaaga
     5401 cctggcacat ttcaacgtgc tggatagagc cgacggaacc cccgacctga cagctctggt
     5461 gaacagggca agagaaatga tggcctacga tcgcaaacgc aaaaacgcag tcccacggag
     5521 tatcctggat atgctggctc ggctggggct gacactgaag tggcagatga aggaccacct
     5581 gctgcaggac gctacaatca cccaggctgc aatcaaacac ctggataaag tgcggctgac
     5641 cgtggggggc cccgccgccg tcaccgaagc acgcttctca caggactacc tgcagatggt
     5701 cgctgcagtg ttcaatggga gcgtgcagaa ccctaaacca cggcgccggg atgatgggga
     5761 cgcctggcac aagccaccca agcctgcaac tgctcagtct cagcctgatc agaaacctcc
     5821 caacaaagca ccttctgcag ggtccaggct gccaccccca caggtcgggg aggtctacga
     5881 aggagtcgtg gtcaaagtca ttgataccgg ctcactgggc tttctggccg tggagggggt
     5941 ggctggcaat attggcctgc atatctcccg cctgcgacga atcagagagg acgccatcat
     6001 tgtcgggcgg agatacagat tcagggtcga aatctatgtc ccccccaaga gcaacacttc
     6061 caaactgaac gccgctgacc tggtgcgaat tgacTGATTG ACAGCTAGCT CAGTCCTAGG
     6121 TATAATGCTA GCGCCTCACA TCACCGCCAA GACGACGGCG GACTGAACGG AGACGGAGAT
     6181 TCGTCTCGGC CTCACATCAC CGCCAAGACG ACGGCGGACT GAACaagctt taatgcggta
     6241 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc
     6301 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
     6361 tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg
     6421 tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt
     6481 ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact
     6541 acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg
     6601 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     6661 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     6721 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     6781 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     6841 gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6901 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6961 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     7021 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     7081 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     7141 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     7201 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     7261 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa
     7321 cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
     7381 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
     7441 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
     7501 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac
     7561 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat
     7621 ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     7681 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     7741 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     7801 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct gctgaagttg
     7861 cccgcaacag agagtggaac caaccggtga taccacgata ctatgactga gagtcaacgc
     7921 catgagcggc ctcatttctt attctgagtt acaacagtcc gcaccgctgt ccggtagctc
     7981 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     8041 gcaactcgta ggacaggtgc cggcagcgcc caacagtccc ccggccacgg ggcctgccac
     8101 catacccacg ccgaaacaag cgccctgcac cattatgttc cggatctgca tcgcaggatg
     8161 ctgctggcta ccctgtggaa ca
//