LOCUS       pR0035.14_pLac_C2C2.15  8185 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6172..6191
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2240..6097
                     /label="Rc C2c2-15"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6101..6135
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6192..6227
                     /label="DR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6285..7475
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    5393..5395
                     /label="R1052"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3629..3631
                     /label="R464"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6136..6171
                     /label="DR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgcagattgg gaaagtccag ggcagaacca ttagcgagtt
     2281 tggcgacccc gcaggagggc tgaaaaggaa gattagcacc gacggaaaga accggaaaga
     2341 actgcccgcc catctgtcaa gcgatccaaa ggctctgatc ggccagtgga ttagcggcat
     2401 tgataagatc tacaggaagc ctgattcaag aaagagcgat ggaaaagcca tccatagccc
     2461 aactccaagc aagatgcagt ttgatgccag ggacgacctg ggggaagcat tctggaaact
     2521 ggtgtcagag gctgggctgg ctcaggacag cgattacgat cagtttaaaa ggcggctgca
     2581 tccctatggg gataagtttc agcccgccga tagcggcgct aaactgaaat tcgaagctga
     2641 tcctccagag ccccaggctt ttcacggccg ctggtatggg gcaatgagca aaagaggaaa
     2701 cgacgctaaa gaactggcag cagcactgta tgaacatctg catgtcgatg aaaaacgaat
     2761 tgatggccag ccaaaaagga acccaaagac agataaattc gcaccagggc tggtcgtggc
     2821 cagggcactg gggatcgaga gctctgtgct gccacggggg atggcaagac tggcacggaa
     2881 ttggggggaa gaggaaatcc agacatactt cgtggtcgac gtggcagcaa gcgtgaagga
     2941 agtggcaaag gcagccgtga gcgccgcaca ggccttcgac ccacctcggc aggtgtccgg
     3001 caggtcactg agcccaaagg tcgggtttgc cctggctgaa catctggagc gggtcacagg
     3061 ctccaaaagg tgcagcttcg atcctgcagc tgggccaagc gtcctggccc tgcacgacga
     3121 agtcaagaaa acctataaac ggctgtgcgc ccggggaaag aacgccgcaa gagcctttcc
     3181 agcagacaaa acagaactgc tggccctgat gcggcatacc cacgagaacc gggtgaggaa
     3241 tcagatggtc agaatggggc gggtgagtga gtataggggg cagcaggcag gggacctggc
     3301 acagagccac tactggacaa gcgcaggaca gaccgaaatc aaggagtcag agatttttgt
     3361 ccgcctgtgg gtcggggctt tcgcactggc cgggaggtca atgaaagcct ggattgatcc
     3421 catgggaaag atcgtcaaca ctgaaaagaa cgatcgcgac ctgaccgctg cagtcaacat
     3481 tcggcaggtc atcagcaaca aggagatggt ggccgaagct atggcacgac gggggatcta
     3541 ctttggagaa accccagagc tggataggct gggagcagaa ggcaatgagg gcttcgtctt
     3601 tgcactgctg agatacctga gagggtgcag gaatcagacc tttcacctgg gggccagggc
     3661 agggttcctg aaagagatta gaaaagaact ggaaaaaacc cggtggggga aggccaagga
     3721 ggcagagcac gtggtcctga ccgataaaac tgtcgctgcc atcagagcca tcatcgacaa
     3781 cgatgccaag gcactggggg cccggctgct ggcagacctg tcaggggctt ttgtcgctca
     3841 ctatgccagt aaagaacact ttagcaccct gtactcagaa attgtcaagg ctgtgaagga
     3901 cgcaccagag gtgtcatccg ggctgccccg gctgaagctg ctgctgaaga gggcagacgg
     3961 agtcagaggc tatgtccatg ggctgaggga tacaaggaaa cacgcctttg ctactaaact
     4021 gccaccacct ccagcaccaa gggaactgga tgacccagcc accaaagcca gatacatcgc
     4081 actgctgaga ctgtacgatg gaccattcag ggcttatgca tccggcatta ctgggacagc
     4141 tctggccggg ccagctgcac gagccaagga ggccgcaacc gctctggcac agtccgtgaa
     4201 tgtcacaaaa gcatacagcg atgtgatgga gggcaggtca agcagactga ggccacccaa
     4261 cgatggggaa acactgagag aatacctgag cgcactgaca ggagaaacag caacagaatt
     4321 tcgggtccag attggctatg aaagcgactc agagaatgct cggaagcagg ccgagttcat
     4381 tgagaactat agacgggata tgctggcttt tatgtttgag gactacatcc gggctaaggg
     4441 gttcgactgg attctgaaaa tcgagcccgg agcaactgca atgacacggg cacctgtgct
     4501 gcccgagcca attgacaccc gggggcagta cgaacactgg caggcagcac tgtatctggt
     4561 aatgcatttc gtgcccgcat cagatgtcag caacctgctg catcagctga ggaagtggga
     4621 ggccctgcag gggaaatatg aactggtgca ggatggcgac gcaacagatc aggcagacgc
     4681 acgacgagag gccctggatc tggtcaagcg atttcgcgat gtcctggtgc tgttcctgaa
     4741 aacaggagag gcccggttcg agggacgggc agctccattc gacctgaaac ccttccgagc
     4801 actgttcgca aatccagcaa ctttcgatcg gctgtttatg gctacaccta ccacagcaag
     4861 gccagcagag gatgatcccg agggggacgg cgcatcagaa ccagagctga gggtggcccg
     4921 gactctgaga gggctgcggc agattgccag gtataatcac atggcagtcc tgagcgatct
     4981 gtttgcaaag cacaaagtcc gagatgagga agtcgcaagg ctggcagaaa ttgaagatga
     5041 gactcaggag aaatcacaga tcgtcgctgc acaggaactg agaaccgacc tgcatgacaa
     5101 agtcatgaag tgtcatccca aaaccatctc tcctgaagaa agacagtcct acgccgcagc
     5161 tattaagacc atcgaagagc ataggtttct ggtggggagg gtctatctgg gagatcatct
     5221 gcggctgcac agactgatga tggatgtgat tggacggctg atcgattacg ctggagccta
     5281 cgagagggat actgggacat ttctgatcaa tgcctcaaag cagctggggg ccggcgcaga
     5341 ctgggcagtg acaattgccg gggcagcaaa cactgacgcc aggactcaga ctcggaagga
     5401 cctggcccac tttaacgtgc tggatagggc cgacgggaca cctgacctga ccgccctggt
     5461 gaatcgagca agagaaatga tggcatacga tcggaaaaga aaaaacgcag tcccaagatc
     5521 aattctggat atgctggcca ggctgggact gactctgaaa tggcagatga aggatcacct
     5581 gctgcaggat gcaactatta cccaggctgc aattaagcat ctggacaaag tgcggctgac
     5641 agtgggcggg cctgcagctg tgactgaggc acgattctcc caggactacc tgcagatggt
     5701 ggccgcagtg tttaacgggt ctgtccagaa tccaaagcca aggaggaggg acgacggcga
     5761 cgcatggcat aaaccaccta agccagcaac tgcacagtcc cagcccgacc agaagccacc
     5821 caacaaagca cctagcgcag ggtcacggct gcccccacca caggtcgggg aagtctatga
     5881 aggggtggtg gtgaaagtga tcgataccgg gtcactggga ttcctggcag tcgaaggggt
     5941 cgcagggaat atcggcctgc atattagtag gctgaggaga atcagagaag atgctattat
     6001 cgtgggcagg agatacaggt ttcgggtcga gatctacgtg ccccccaagt ctaatacctc
     6061 caaactgaac gctgctgacc tggtgcgaat tgacggaTGA TTGACAGCTA GCTCAGTCCT
     6121 AGGTATAATG CTAGCGCCTC ACATCACCGC CAAGACGACG GCGGACTGAA CGGAGACGGA
     6181 GATTCGTCTC GGCCTCACAT CACCGCCAAG ACGACGGCGG ACTGAACaag ctttaatgcg
     6241 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     6301 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     6361 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     6421 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     6481 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     6541 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     6601 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     6661 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     6721 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     6781 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     6841 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     6901 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     6961 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     7021 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     7081 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     7141 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     7201 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     7261 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     7321 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     7381 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     7441 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     7501 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     7561 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     7621 catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     7681 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     7741 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     7801 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcccctacgt gctgctgaag
     7861 ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg atactatgac tgagagtcaa
     7921 cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc tgtccggtag
     7981 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     8041 catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca cggggcctgc
     8101 caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct gcatcgcagg
     8161 atgctgctgg ctaccctgtg gaaca
//