LOCUS       pR0035.15_pLac_C2C2.16  8185 bp ds-DNA     circular     04-JUL-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1613..2158
                     /label="ori"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3629..3631
                     /label="R464"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2232..2239
                     /label="Eco31I recognition and cut site"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    1208..1348
                     /label="part of IS1 from Tn9"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2196..2225
                     /label="lac promoter"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6285..7475
                     /label="TcR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    5393..5395
                     /label="R1052"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2226..2231
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     misc_feature    6192..6227
                     /label="DR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2240..6097
                     /label="Rc C2c2-16"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6101..6135
                     /label="J23119 Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6136..6171
                     /label="DR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6172..6191
                     /label="BsmBI insertion site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(328..984)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
ORIGIN
        1 cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa
       61 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
      121 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
      181 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
      241 atttctgcca ttcatccgct tattatcact tattcaggcg tagcAaccag gcgtttaagg
      301 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      361 taattcatta agcattctgc cgacatggaa gccatcacaA ACggcatgat gaacctgaat
      421 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
      481 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
      541 attggctgaC ACgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
      601 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
      661 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
      721 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
      781 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
      841 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
      901 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
      961 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1021 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1081 ccgatcaacg tgtcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1141 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1201 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1261 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1321 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1381 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     1441 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1501 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     1561 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     1621 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1741 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1801 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1861 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1921 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     1981 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2041 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2101 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2161 agatcatctt attaatcaga taaaatattt ctagatttac actttatgct tccggctcgt
     2221 atgttAGGAG Gtcttatcca tgcagatcgg caaagtgcag ggaaggacta ttagcgagtt
     2281 cggggaccca gcaggaggac tgaagaggaa gatttctact gacggaaaga accggaaaga
     2341 actgccagct cacctgtcaa gcgatccaaa ggctctgatt ggccagtgga ttagtggcat
     2401 tgacaagatc taccggaaac cagatagcag aaaatcagac ggcaaggcta tccacagtcc
     2461 tacaccaagc aaaatgcagt tcgacgcccg ggatgatctg ggggaagcat tctggaaact
     2521 ggtgagtgaa gcagggctgg cacaggactc agattatgat cagtttaagc gcaggctgca
     2581 cccttatggg gacaagtttc agcccgctga cagcggcgca aaactgaagt tcgaggccga
     2641 tccccctgag ccacaggctt tccacggacg ctggtatggg gccatgtcaa agagggggaa
     2701 cgacgcaaag gaactggcag ctgcactgta tgagcatctg catgtggacg aaaaaaggat
     2761 tgatggccag ccaaagagga acccaaagac tgataaattt gcccccgggc tggtggtcgc
     2821 aagggcactg gggatcgaga gctcagtgct gccaaggggg atggccaggc tggccaggaa
     2881 ctggggagag gaggaaattc agacttattt cgtcgtggac gtggcagcct cagtgaagga
     2941 agtggccaag gctgccgtgt ccgctgctca ggcatttgac ccacctcgac aggtgtcagg
     3001 acggagcctg tctccaaagg tcgggtttgc cctggcagag cacctggaaa gggtcaccgg
     3061 aagcaaaaga tgcagctttg atccagcagc tgggccaagt gtcctggcac tgcacgacga
     3121 ggtgaaaaag acctataagc gactgtgcgc cagagggaaa aatgccgcaa gagcttttcc
     3181 agcagacaag acagagctgc tggcactgat gagacacaca cacgaaaata gggtgcgcaa
     3241 ccagatggtc aggatgggga gggtcagcga gtatcgggga cagcaggccg gggatctggc
     3301 tcagagccat tattggacta gcgcagggca gaccgagatt aaggagagcg aaatcttcgt
     3361 gcggctgtgg gtcggggcat ttgccctggc aggccgatcc atgaaggcct ggatcgaccc
     3421 tatggggaag atcgtgaaca ctgagaagaa cgaccgggac ctgactgcag cagtgaacat
     3481 caggcaggtc atcagcaata aggaaatggt cgccgaggca atggctagaa ggggaatcta
     3541 cttcggggag actccagagc tggaccgact gggggcagaa ggaaacgagg ggttcgtgtt
     3601 cgctctgctg cgatacctga ggggctgtag aaatcagaca tttcatctgg gggcaagagc
     3661 tggctttctg aaagagattc ggaaagagct ggaaaagacc cggtggggga aggccaaaga
     3721 ggcagagcat gtggtcctga cagacaaaac tgtggcagct atccgagcta tcatcgacaa
     3781 tgatgccaaa gcactggggg ccagactgct ggcagatctg tccggcgcct ttgtcgccca
     3841 ctacgcaagc aaggagcact tttctacact gtattctgag atcgtcaagg ctgtgaagga
     3901 tgcacctgaa gtcagttcag gactgcccag gctgaagctg ctgctgaaaa gggccgatgg
     3961 ggtgcgcggg tatgtccacg ggctgaggga tacaagaaaa catgccttcg caacaaagct
     4021 gccaccccca cctgccccaa gagagctgga cgatcccgca accaaggcca ggtacatcgc
     4081 cctgctgcgc ctgtatgacg gcccattcag agcttacgca tccgggatta cagggactgc
     4141 actggctggc ccagcagcta gggccaaaga ggcagcaaca gctctggctc agtccgtgaa
     4201 tgtcactaag gcctactctg acgtgatgga aggacggtca tcacggctga ggcctcccaa
     4261 tgacggagag actctgagag aatacctgtc tgcactgact ggggaaactg caacagagtt
     4321 tcgggtccag atcggatacg aaagcgacag cgaaaacgca agaaaacagg ctgagtttat
     4381 tgagaattac cggagagata tgctggcttt tatgttcgag gattatatca gagccaaggg
     4441 gttcgattgg attctgaaaa ttgagcccgg ggcaacagcc atgactaggg caccagtgct
     4501 gcccgaacct atcgatacac gagggcagta cgagcattgg caggctgcac tgtatctggt
     4561 catgcatttc gtgccagcct cagacgtgtc taacctgctg caccagctgc ggaaatggga
     4621 agccctgcag gggaagtacg agctggtcca ggacggggac gccacagacc aggccgacgc
     4681 ccggcgggaa gctctggacc tggtcaagcg gttccgagat gtcctggtgc tgttcctgaa
     4741 aactggcgag gcacgcttcg agggaagggc tgctcctttt gacctgaagc cattccgagc
     4801 tctgtttgcc aaccccgcaa cattcgatcg cctgtttatg gcaacaccta ccacagctcg
     4861 accagccgag gatgatccag agggggacgg ggcatccgag cctgaactgc gggtcgcaag
     4921 gacactgcgg gggctgcggc agatcgcacg gtacaatcac atggcagtcc tgtcagacct
     4981 gttcgccaaa cataaggtgc gcgatgaaga ggtggcaagg ctggccgaga ttgaggatga
     5041 aacccaggag aagtcacaga tcgtcgccgc acaggagctg cggaccgacc tgcacgacaa
     5101 ggtcatgaag tgccacccta agacaattag ccccgaggag cgccagagct acgcagcagc
     5161 catcaaaact atcgaagagc atagatttct ggtggggagg gtctatctgg gggatcatct
     5221 gcgactgcac cggctgatga tggacgtgat cggccgcctg attgactatg ctggagccta
     5281 tgaaagagat actggcacat tcctgatcaa tgcaagcaaa cagctggggg ccggcgcaga
     5341 ctgggcagtg acaatcgccg gggccgccaa tacagatgct agaactcaga cacggaagga
     5401 tctggctcac ttcaacgtgc tggatcgggc tgatgggacc cccgacctga ctgcactggt
     5461 gaatcgcgca cgggaaatga tggcctacga tcggaagaga aaaaatgccg tcccaaggag
     5521 cattctggat atgctggcac ggctgggact gaccctgaag tggcagatga aggatcacct
     5581 gctgcaggat gcaaccatta cccaggcagc tatcaagcac ctggacaagg tgagactgac
     5641 cgtgggcgga ccagcagcag tcacagaagc tcggttctct caggactacc tgcagatggt
     5701 ggctgcagtg tttaacggat cagtgcagaa ccccaagcca cggcgcaggg acgacggaga
     5761 tgcttggcat aaacctccta agccagccac cgcccagagc cagccagatc agaaacctcc
     5821 caacaaagca ccttccgcag ggtcccggct gccccctcca caggtgggag aggtctacga
     5881 aggcgtggtc gtcaaagtca ttgatactgg atctctgggg ttcctggctg tcgaaggcgt
     5941 cgctggaaat attggcctgc acatctcccg actgaggcgg attcgggagg atgctatcat
     6001 tgtcggacga aggtataggt tccgggtgga aatctacgtg cctccaaaga gcaacacttc
     6061 caaactgaac gctgccgacc tggtgcggat tgatggaTGA TTGACAGCTA GCTCAGTCCT
     6121 AGGTATAATG CTAGCGCCTC ACATCACCGC CAAGACGACG GCGGACTGAA CGGAGACGGA
     6181 GATTCGTCTC GGCCTCACAT CACCGCCAAG ACGACGGCGG ACTGAACaag ctttaatgcg
     6241 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     6301 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     6361 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     6421 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     6481 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     6541 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     6601 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     6661 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     6721 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     6781 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     6841 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     6901 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     6961 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     7021 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     7081 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     7141 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     7201 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     7261 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     7321 taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     7381 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     7441 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     7501 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     7561 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     7621 catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     7681 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     7741 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     7801 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcccctacgt gctgctgaag
     7861 ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg atactatgac tgagagtcaa
     7921 cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc tgtccggtag
     7981 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     8041 catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca cggggcctgc
     8101 caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct gcatcgcagg
     8161 atgctgctgg ctaccctgtg gaaca
//