LOCUS       pR0174_pFUGW_EFS-NLS-L 13088 bp ds-DNA     circular     11-OCT-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    7821..7826
                     /label="GS linker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     3073..3092
                     /label="HKO074"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(11983..12665)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1286..1305
                     /label="HKO084"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     LTR             9551..9739
                     /label="3' LTR"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3552..3599
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3606..7061
                     /label="LwC2c2-3"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(11595..11615)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      12188..12413
                     /label="ColE1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     8524..8543
                     /label="HKO083"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    7827..7874
                     /label="NLS"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        979..1282
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    8443..9036
                     /label="WPRE; Woodchuck post-transcriptional"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(11647..11677)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     PCR_primer      1070..1076
                     /label="ACGCCAA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     PCR_primer      8022..8045
                     /label="ATGGCCAAGCCTTTGTCTCAAGAA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     9296..9316
                     /label="HKO085"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             8022..8420
                     /label="Bla"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      10057..10363
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     LTR             1493..1681
                     /label="5' LTR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     PCR_primer      complement(8398..8420)
                     /label="TTAGCCCTCCCACACATAACCAG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1732..1869
                     /label="Psi"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    7875..7955
                     /label="HA tag 3x"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7062..7106
                     /label="Linker (GGS)5"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PCR_primer      complement(3508..3512)
                     /label="CCTGTGTTCTGGCGGCAAACC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    7978..8021
                     /label="P2A"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7107..7820
                     /label="msfGFP"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3600..3605
                     /label="GS linker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(12763..334)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        3273..3512
                     /label="EFS-NS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     PCR_primer      3273..3298
                     /label="TAGGTCTTGAAAGGAGTGGGAATTGG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3105..3222
                     /label="CPPT; central polypurine tract"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1789..1792
                     /label="SD; splice donor"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_binding    complement(11621..11643)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
       61 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
      121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
      181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
      241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
      301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
      361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
      421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      661 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
      721 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      781 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      841 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      901 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      961 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1021 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1081 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     1141 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1201 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1261 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1321 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1381 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1441 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     1501 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1561 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1621 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1681 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1741 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1801 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1861 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1921 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1981 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     2041 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     2101 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     2161 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     2221 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     2281 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     2341 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     2401 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     2461 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     2521 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     2581 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     2641 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2701 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2761 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2821 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2881 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2941 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     3001 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     3061 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     3121 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3181 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     3241 agcagagatc cagtttggtt aattaagcta gctaggtctt gaaaggagtg ggaattggct
     3301 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     3361 gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     3421 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     3481 tcgccgtgaa cgttcttttt cgcaacgggt ttgccagaac acaggaccgg ttctagagcg
     3541 ctgccaccAT Gaagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag
     3601 gatccatgaa agtgaccaag gtcgacggCA TCAGCCACAA GAAGTACATC GAAGAGGGCA
     3661 AGCTCGTGAA GTCCACCAGC GAGGAAAACC GGACCAGCGA GAGACTGAGC GAGCTGCTGA
     3721 GCATCCGGCT GGACATCTAC ATCAAGAACC CCGACAACGC CTCCGAGGAA GAGAACCGGA
     3781 TCAGAAGAGA GAACCTGAAG AAGTTCTTTA GCAACAAGGT GCTGCACCTG AAGGACAGCG
     3841 TGCTGTATCT GAAGAACCGG AAAGAAAAGA ACGCCGTGCA GGACAAGAAC TATAGCGAAG
     3901 AGGACATCAG CGAGTACGAC CTGAAAAACA AGAACAGCTT CTCCGTGCTG AAGAAGATCC
     3961 TGCTGAACGA GGACGTGAAC TCTGAGGAAC TGGAAATCTT TCGGAAGGAC GTGGAAGCCA
     4021 AGCTGAACAA GATCAACAGC CTGAAGTACA GCTTCGAAGA GAACAAGGCC AACTACCAGA
     4081 AGATCAACGA GAACAACGTG GAAAAAGTGG GCGGCAAGAG CAAGCGGAAC ATCATCTACG
     4141 ACTACTACAG AGAGAGCGCC AAGCGCAACG ACTACATCAA CAACGTGCAG GAAGCCTTCG
     4201 ACAAGCTGTA TAAGAAAGAG GATATCGAGA AACTGTTTTT CCTGATCGAG AACAGCAAGA
     4261 AGCACGAGAA GTACAAGATC CGCGAGTACT ATCACAAGAT CATCGGCCGG AAGAACGACA
     4321 AAGAGAACTT CGCCAAGATT ATCTACGAAG AGATCCAGAA CGTGAACAAC ATCAAAGAGC
     4381 TGATTGAGAA GATCCCCGAC ATGTCTGAGC TGAAGAAAAG CCAGGTGTTC TACAAGTACT
     4441 ACCTGGACAA AGAGGAACTG AACGACAAGA ATATTAAGTA CGCCTTCTGC CACTTCGTGG
     4501 AAATCGAGAT GTCCCAGCTG CTGAAAAACT ACGTGTACAA GCGGCTGAGC AACATCAGCA
     4561 ACGATAAGAT CAAGCGGATC TTCGAGTACC AGAATCTGAA AAAGCTGATC GAAAACAAAC
     4621 TGCTGAACAA GCTGGACACC TACGTGCGGA ACTGCGGCAA GTACAACTAC TATCTGCAAG
     4681 TGGGCGAGAT CGCCACCTCC GACTTTATCG CCCGGAACCG GCAGAACGAG GCCTTCCTGA
     4741 GAAACATCAT CGGCGTGTCC AGCGTGGCCT ACTTCAGCCT GAGGAACATC CTGGAAACCG
     4801 AGAACGAGAA CGGTATCACC GGCCGGATGC GGGGCAAGAC CGTGAAGAAC AACAAGGGCG
     4861 AAGAGAAATA CGTGTCCGGC GAGGTGGACA AGATCTACAA TGAGAACAAG CAGAACGAAG
     4921 TGAAAGAAAA TCTGAAGATG TTCTACAGCT ACGACTTCAA CATGGACAAC AAGAACGAGA
     4981 TCGAGGACTT CTTCGCCAAC ATCGACGAGG CCATCAGCAG CATCAGACAC GGCATCGTGC
     5041 ACTTCAACCT GGAACTGGAA GGCAAGGACA TCTTCGCCTT CAAGAATATC GCCCCCAGCG
     5101 AGATCTCCAA GAAGATGTTT CAGAACGAAA TCAACGAAAA GAAGCTGAAG CTGAAAATCT
     5161 TCAAGCAGCT GAACAGCGCC AACGTGTTCA ACTACTACGA GAAGGATGTG ATCATCAAGT
     5221 ACCTGAAGAA TACCAAGTTC AACTTCGTGA ACAAAAACAT CCCCTTCGTG CCCAGCTTCA
     5281 CCAAGCTGTA CAACAAGATT GAGGACCTGC GGAATACCCT GAAGTTTTTT TGGAGCGTGC
     5341 CCAAGGACAA AGAAGAGAAG GACGCCCAGA TCTACCTGCT GAAGAATATC TACTACGGCG
     5401 AGTTCCTGAA CAAGTTCGTG AAAAACTCCA AGGTGTTCTT TAAGATCACC AATGAAGTGA
     5461 TCAAGATTAA CAAGCAGCGG AACCAGAAAA CCGGCCACTA CAAGTATCAG AAGTTCGAGA
     5521 ACATCGAGAA AACCGTGCCC GTGGAATACC TGGCCATCAT CCAGAGCAGA GAGATGATCA
     5581 ACAACCAGGA CAAAGAGGAA AAGAATACCT ACATCGACTT TATTCAGCAG ATTTTCCTGA
     5641 AGGGCTTCAT CGACTACCTG AACAAGAACA ATCTGAAGTA TATCGAGAGC AACAACAACA
     5701 ATGACAACAA CGACATCTTC TCCAAGATCA AGATCAAAAA GGATAACAAA GAGAAGTACG
     5761 ACAAGATCCT GAAGAACTAT GAGAAGCACA ATCGGAACAA AGAAATCCCT CACGAGATCA
     5821 ATGAGTTCGT GCGCGAGATC AAGCTGGGGA AGATTCTGAA GTACACCGAG AATCTGAACA
     5881 TGTTTTACCT GATCCTGAAG CTGCTGAACC ACAAAGAGCT GACCAACCTG AAGGGCAGCC
     5941 TGGAAAAGTA CCAGTCCGCC AACAAAGAAG AAACCTTCAG CGACGAGTTG GAACTGATCA
     6001 ACCTGCTGAA CCTGGACAAC AACAGAGTGA CCGAGGACTT CGAGCTGGAA GCCAACGAGA
     6061 TCGGCAAGTT CCTGGACTTC AACGAAAACA AAATCAAGGA CCGGAAAGAG CTGAAAAAGT
     6121 TCGACACCAA CAAGATCTAT TTCGACGGCG AGAACATCAT CAAGCACCGG GCCTTCTACA
     6181 ATATCAAGAA ATACGGCATG CTGAATCTGC TGGAAAAGAT CGCCGATAAG GCCAAGTATA
     6241 AGATCAGCCT GAAAGAACTG AAAGAGTACA GCAACAAGAA GAATGAGATT GAAAAGAACT
     6301 ACACCATGCA GCAGAACCTG CACCGGAAGT ACGCCAGACC CAAGAAGGAC GAAAAGTTCA
     6361 ACGACGAGGA CTACAAAGAG TATGAGAAGG CCATCGGCAA CATCCAGAAG TACACCCACC
     6421 TGAAGAACAA GGTGGAATTC AATGAGCTGA ACCTGCTGCA GGGCCTGCTG CTGAAGATCC
     6481 TGCACCGGCT CGTGGGCTAC ACCAGCATCT GGGAGCGGGA CCTGAGATTC CGGCTGAAGG
     6541 GCGAGTTTCC CGAGAACCAC TACATCGAGG AAATTTTCAA TTTCGACAAC TCCAAGAATG
     6601 TGAAGTACAA AAGCGGCCAG ATCGTGGAAA AGTATATCAA CTTCTACAAA GAACTGTACA
     6661 AGGACAATGT GGAAAAGCGG AGCATCTACT CCGACAAGAA AGTGAAGAAA CTGAAGCAGG
     6721 AAAAAAAGGA CCTGTACATC CGGAACTACA TTGCCCACTT CAACTACATC CCCCACGCCG
     6781 AGATTAGCCT GCTGGAAGTG CTGGAAAACC TGCGGAAGCT GCTGTCCTAC GACCGGAAGC
     6841 TGAAGAACGC CATCATGAAG TCCATCGTGG ACATTCTGAA AGAATACGGC TTCGTGGCCA
     6901 CCTTCAAGAT CGGCGCTGAC AAGAAGATCG AAATCCAGAC CCTGGAATCA GAGAAGATCG
     6961 TGCACCTGAA GAATCTGAAG AAAAAGAAAC TGATGACCGA CCGGAACAGC GAGGAACTGT
     7021 GCGAACTCGT GAAAGTCATG TTCGAGTACA AGGCCCTGGA AGGAGGAGGT GGAAGCGGAG
     7081 GAGGAGGAAG CGGAGGAGGA GGTAGCGTGA GTAAAGGTGA AGAACTCTTC ACTGGAGTAG
     7141 TGCCCATTCT GGTAGAGCTT GATGGAGATG TAAATGGACA TAAATTCTCC GTCAGGGGCG
     7201 AAGGCGAAGG GGACGCCACG AATGGTAAGC TGACTCTGAA ATTCATCTGT ACGACGGGCA
     7261 AACTGCCCGT CCCATGGCCT ACACTCGTAA CGACCCTCAC CTACGGCGTG CAATGCTTTT
     7321 CTCGATATCC CGACCACATG AAACAGCATG ACTTTTTCAA GTCTGCAATG CCTGAAGGTT
     7381 ATGTTCAAGA AAGGACCATC AGCTTTAAGG ATGATGGTAC ATATAAAACC CGAGCCGAGG
     7441 TTAAATTTGA AGGGGACACT CTGGTTAATC GAATTGAACT GAAAGGTATT GATTTTAAGG
     7501 AGGACGGTAA CATACTGGGG CACAAGTTGG AGTACAACTT TAACAGCCAT AATGTGTATA
     7561 TTACCGCTGA TAAGCAGAAA AATGGGATAA AGGCCAACTT TAAGATCCGA CATAATGTCG
     7621 AAGATGGTAG TGTTCAACTG GCTGATCATT ACCAACAAAA TACGCCCATC GGAGATGGAC
     7681 CTGTACTCTT GCCTGACAAT CATTATCTCT CCACGCAATC AAAGCTTTCC AAGGACCCAA
     7741 ACGAAAAGAG AGATCACATG GTCCTTCTGG AATTTGTGAC TGCCGCAGGC ATCactctcg
     7801 gtatggatga gctgtacaag ggatccaagc gacctgccgc cacaaagaag gctggacagg
     7861 ctaagaagaa gaaataccca tacgatgttc cagattacgc ttatccctac gacgtgcctg
     7921 attatgcata cccatatgat gtccccgact atgccggatc cggcgcaaca aacttctCTC
     7981 TGCTGAAACA AGCCGGAGAT GTCGAAGAGA ATCCTGGACC Gatggccaag cctttgtctc
     8041 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     8101 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     8161 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     8221 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     8281 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     8341 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     8401 gttatgtgtg ggagggctaa gaattcgata tcaagcttat cggtaatcaa cctctggatt
     8461 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8521 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8581 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8641 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8701 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8761 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8821 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     8881 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8941 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9001 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     9061 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     9121 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     9181 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9241 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     9301 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     9361 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     9421 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9481 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     9541 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9721 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     9781 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9841 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9901 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9961 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    10021 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    10081 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    10141 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10201 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10261 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10321 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10381 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10441 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    10501 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    10561 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10621 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    10681 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10741 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10801 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
    10861 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    10921 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    10981 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    11041 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    11101 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    11161 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    11221 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    11281 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    11341 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    11401 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    11461 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11521 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    11581 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    11641 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    11701 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11761 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    11821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    12001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    12061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    12121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    12181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    12241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    12301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    12361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    12481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    13021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    13081 catcgtgg
//