LOCUS       Exported                6702 bp ds-DNA     circular SYN 20-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p5A0-T
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Mark Politz
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 21, 2017 from SnapGene 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..816)
                     /codon_start=1
                     /note="kanR"
                     /note="kanamycin resistance gene from TN903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(913..1575)
                     /codon_start=1
                     /note="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number 
                     X66730"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     terminator      2258..2301
                     /note="terminator"
     CDS             complement(2349..2972)
                     /note="TetR"
     RBS             complement(2979..2983)
                     /note="RBS"
     misc_feature    2992..2997
                     /note="-35"
     protein_bind    2997..3015
                     /note="tet operator"
     misc_feature    complement(2997..3002)
                     /note="-10"
     misc_feature    3016..3021
                     /note="-10"
     misc_feature    complement(3020..3025)
                     /note="-35"
     protein_bind    3027..3045
                     /note="tet operator"
     RBS             3068..3073
                     /note="RBS"
     gene            3084..3833
                     /note="TEV Protease"
     misc_feature    3084..3107
                     /note="His 7x Tag"
     misc_feature    3762..3764
                     /note="S219V"
                     /note="S219V, this version of TEV does not autocatalyze and
                     inactivate itself and is more efficient than wildtype (ie 
                     with Serine)"
     misc_feature    3816..3830
                     /note="poly-arginine tag"
                     /note="poly-arginine tag, purification by cation-exchange 
                     chromatography"
     terminator      complement(3902..3938)
                     /note="SoxR terminator"
                     /note="SoxR terminator (what is the directionality of this 
                     terminator?)"
     promoter        4011..4045
                     /note="J23102 promoter"
     misc_feature    4011..4016
                     /note="-35"
     misc_feature    4034..4039
                     /note="-10"
     RBS             4072..4077
                     /note="RBS"
     gene            4086..6620
                     /note="TALElacO1"
     misc_feature    4086..4514
                     /note="TALE N-terminus"
     misc_feature    4515..4616
                     /note="Repeat 1 (NG)"
     repeat_region   4548..4553
                     /note="NI"
     misc_feature    4617..4718
                     /note="Repeat 2 (NN)"
     repeat_region   4650..4655
                     /note="NN"
     misc_feature    4719..4820
                     /note="Repeat 3 (NG)"
     repeat_region   4752..4757
                     /note="NG"
     misc_feature    4821..4922
                     /note="Repeat 4 (NN)"
     repeat_region   4854..4859
                     /note="NN"
     misc_feature    4923..5024
                     /note="Repeat 5 (NI)"
     repeat_region   4956..4961
                     /note="NI"
     misc_feature    5025..5126
                     /note="Repeat 6 (NN)"
     repeat_region   5058..5063
                     /note="NN"
     misc_feature    5127..5228
                     /note="Repeat 7 (HD)"
     repeat_region   5160..5165
                     /note="HD"
     misc_feature    5229..5330
                     /note="Repeat 8 (NH)"
     repeat_region   5262..5267
                     /note="NH"
     misc_feature    5331..5432
                     /note="Repeat 9 (NN)"
     repeat_region   5364..5369
                     /note="NN"
     misc_feature    5433..5534
                     /note="Repeat 10 (NI)"
     repeat_region   5466..5471
                     /note="NI"
     misc_feature    5535..5636
                     /note="Repeat 11 (NG)"
     repeat_region   5568..5573
                     /note="NG"
     misc_feature    5637..5738
                     /note="Repeat 12 (NI)"
     repeat_region   5670..5675
                     /note="NI"
     misc_feature    5739..5840
                     /note="Repeat 13 (NI)"
     repeat_region   5772..5777
                     /note="NI"
     misc_feature    5841..5942
                     /note="Repeat 14 (HD)"
     repeat_region   5874..5879
                     /note="HD"
     misc_feature    5943..6044
                     /note="Repeat 15 (NI)"
     repeat_region   5976..5981
                     /note="NI"
     misc_feature    6045..6146
                     /note="Repeat 16 (NI)"
     repeat_region   6078..6083
                     /note="NI"
     misc_feature    6147..6248
                     /note="Repeat 17 (NG)"
     repeat_region   6180..6185
                     /note="NG"
     misc_feature    6249..6308
                     /note="0.5 repeat (NG)"
     repeat_region   6282..6287
                     /note="NG"
     misc_feature    6309..6593
                     /note="C-terminus"
     misc_feature    6594..6617
                     /note="FLAG tag"
     terminator      complement(6655..6686)
                     /note="TonB terminator"
ORIGIN
        1 tcagaaaaac tcatcgagca tcaagtgaaa ctgcaattta ttcatatcag gattatcaat
       61 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
      121 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
      181 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
      241 tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca
      301 gccattacgc tcgtcatcaa aatcactcgc accaaccaaa ccgttattca ttcgtgattg
      361 cgcctgagcg agacgaaata cgcgatcgcc gttaaaagga caattacaaa caggaatcga
      421 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
      481 ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc
      541 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
      601 tagcctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
      661 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
      721 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
      781 cctcgagcaa gacgtttccc gttgaatatg gctcatttta gcttccttag ctcctgaaaa
      841 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      901 acctcttacg tactaccggc gcggcagcgt gacccgtgtc ggcggctcca acggctcgcc
      961 atcgtccaga aaacacggct catcgggcat cggcaggcgc tgctgcccgc gccgttccca
     1021 ttcctccgtt tcggtcaagg ctggcaggtc tggttccatg cccggaatgc cgggctggct
     1081 gggcggctcc tcgccggggc cggtcggtag ttgctgctcg cccggataca gggtcgggat
     1141 gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac
     1201 ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc
     1261 attgaccacg taggccgaca cggtgccggg gccgttgagc ttcacgacgg agatccagcg
     1321 ctcggccacc aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt
     1381 ggaaagtgtc ttctggctga ccaccacggc gttctggtgg cccatctgcg ccacgaggtg
     1441 atgcagcagc attgccgccg tgggtttcct cgcaataagc ccggcccacg cctcatgcgc
     1501 tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc cgatttctct
     1561 ggactgcgtg gccatgctta tctccatgcg gtagggtgcc gcacggttgc ggcaccatgc
     1621 gcaatcagct gcaacttttc ggcagcgcga caacaattat gcgttgcgta aaagtggcag
     1681 tcaattacag attttcttta acctacgcaa tgagctattg cggggggtgc cgcaatgagc
     1741 tgttgcgtac cccccttttt taagttgttg atttttaagt ctttcgcatt tcgccctata
     1801 tctagttctt tggtgcccaa agaagggcac ccctgcgggg ttcccccacg ccttcggcgc
     1861 ggctccccct ccggcaaaaa gtggcccctc cggggcttgt tgatcgactg cgcggccttc
     1921 ggccttgccc aaggtggcgc tgcccccttg gaacccccgc actcgccgcc gtgaggctcg
     1981 gggggcaggc gggcgggctt cgccttcgac tgcccccact cgcataggct tgggtcgttc
     2041 caggcgcgtc aaggccaagc cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac
     2101 ttggtgtcca accggcaagc gaagcgcgca ggccgcaggc cggaggcttt tccccagaga
     2161 aaattaaaaa aattgatggg gcaaggccgc aggccgcgca gttggagccg gtgggtatgt
     2221 ggtcgaaggc tgggtagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     2281 cgactgagcc tttcgtttta tttgatgcct ggctctagta gtgatctaca ctagcactat
     2341 cagtgttatt aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat
     2401 tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt
     2461 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga
     2521 tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat
     2581 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt
     2641 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt
     2701 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct
     2761 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc
     2821 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg
     2881 gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg
     2941 ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt gttgacactc
     3001 tatcgttgat agagttattt taccactccc tatcagtgat agagaaaaga attcaaaaga
     3061 tcttttaaga aggagatata catatgcatc atcatcatca tcatcatgga gaaagcttgt
     3121 ttaaggggcc gcgtgattac aacccgatat cgagcaccat ttgtcatttg acgaatgaat
     3181 ctgatgggca cacaacatcg ttgtatggta ttggatttgg tcccttcatc attacaaaca
     3241 agcacttgtt tagaagaaat aatggaacac tgttggtcca atcactacat ggtgtattca
     3301 aggtcaagaa caccacgact ttgcaacaac acctcattga tgggagggac atgataatta
     3361 ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct gaaatttaga gagccacaaa
     3421 gggaagagcg catatgtctt gtgacaacca acttccaaac taagagcatg tctagcatgg
     3481 tgtcagacac tagttgcaca ttcccttcat ctgatggcat attctggaag cattggattc
     3541 aaaccaagga tgggcagtgt ggcagtccat tagtatcaac tagagatggg ttcattgttg
     3601 gtatacactc agcatcgaat ttcaccaaca caaacaatta tttcacaagc gtgccgaaaa
     3661 acttcatgga attgttgaca aatcaggagg cgcagcagtg ggttagtggt tggcgattaa
     3721 atgctgactc agtattgtgg gggggccata aagttttcat ggtgaaacct gaagagcctt
     3781 ttcagccagt taaggaagcg actcaactca tgaatcgtcg tcgccgtcgc taagtccatc
     3841 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgcggaa
     3901 caaaactaaa gcgccacaag ggcgctttag tttgttttca gtccagttac gctggagtct
     3961 gaggctcgtc ctgaatgata tcaagcttga attcgcggcc gcttctagag ttgacagcta
     4021 gctcagtcct aggtactgtg ctagctacta gatatcgctc aatactgacc aaggaggtat
     4081 ctacaatgca tcaccatcat caccatgttg atctgcgtac cctgggttat agccagcagc
     4141 agcaagaaaa aatcaaaccg aaagttcgta gcaccgttgc acagcatcat gaagcactgg
     4201 ttggtcatgg ttttacccat gcacatattg ttgcactgag ccagcatccg gcagcactgg
     4261 gcaccgttgc agttaaatat caggatatga ttgcagcact gccggaagca acccatgaag
     4321 caattgttgg tgttggtaaa cagtggtcag gtgcacgtgc cctggaagca ctgctgaccg
     4381 ttgccggtga actgcgtggt ccgcctctgc agctggatac cggtcagctg ctgaaaattg
     4441 caaaacgtgg tggtgttacc gcagttgaag cagttcatgc atggcgtaat gcactgaccg
     4501 gtgcaccgct gaatctgaca ccggaacagg ttgttgcaat tgccagcaat ggtggtggca
     4561 aacaggcact ggaaaccgtt cagcgtctgc tgccggttct gtgtcaggca catggtctga
     4621 cccctgaaca ggtggtggcc attgcaagca ataatggcgg taaacaagcc ctggaaacag
     4681 tgcagcgcct gttaccggtg ctgtgccagg cccatggctt aactccggaa caggtggtag
     4741 cgatcgcatc aaatggcgga ggtaaacagg ccttagaaac cgtacagcgc ttactgccgg
     4801 tgttatgcca ggcgcacggc ctgacgccag aacaggtagt ggcaatcgcc tcaaataatg
     4861 gtggaaaaca ggcgttagag acagtccagc gcctgctgcc tgtattatgt caagcccatg
     4921 gcctgacccc agagcaagtt gttgcgattg caagtaacat tgggggtaaa caggcacttg
     4981 agacagttca acgtttactg cctgtactgt gccaagctca cggtctgact ccggaacaag
     5041 tcgtcgcgat tgcgagtaat aacggtggca aacaagcatt agaaacggtg caacgcctgc
     5101 tgccagttct ttgccaggct cacggtttaa cccctgagca ggttgtagct attgcgagtc
     5161 atgatggtgg taagcaggcg ttggaaactg tgcaaagact gctgcccgtg ttgtgccaag
     5221 cacatggttt aaccccagaa caagtcgtag caatcgcaag caatcatggt ggcaagcaag
     5281 cgcttgaaac agtacagcgt ttattaccgg tactttgtca ggcccacggt cttacaccag
     5341 aacaagttgt ggccatagcc agtaacaatg gcggaaagca ggctctggaa acggtacaac
     5401 gtctgttacc tgttctgtgt caagcgcacg gattaacacc tgaacaagta gttgccattg
     5461 cgtcaaatat cggaggcaag caggccttgg agacagtgca gagattactg ccagtgttgt
     5521 gtcaggctca tggccttaca cccgagcagg tcgtggcaat tgcatctaat ggtggcggta
     5581 agcaagcttt agagactgtt cagagactgc ttcctgtcct gtgccaggca cacggactta
     5641 cgcctgagca agtggttgca atcgcctcta acatcggtgg taagcaagca ctggaaactg
     5701 tccaacgctt acttccggtg ctttgtcaag cacacggctt aacgccagag caggtcgtcg
     5761 ccatagccag caatatcggt ggtaaacagg cccttgaaac ggtccaaaga cttctgccgg
     5821 tcctttgcca agcgcatggg ctgacacctg agcaggtagt cgcgattgcc tcacatgacg
     5881 gtgggaagca ggcattagaa acagttcaaa gattattacc agtcctgtgt caggcgcatg
     5941 ggttaacccc agagcaggta gttgcaatag catccaacat tggcggaaaa caagcgttgg
     6001 aaacggttca gcggttattg cctgttttgt gccaggcgca tggtttgaca cccgagcaag
     6061 tggtagccat agcctcaaat atagggggta aacaagcttt ggagacagta caacggctgc
     6121 ttccagtttt atgtcaggcc catggattga cgcctgaaca agttgtcgct atcgcaagta
     6181 atgggggtgg taaacaagcg cttgaaaccg ttcaacgcct tctgcctgtg ctttgtcagg
     6241 cacatggatt aacacccgaa caggttgtcg cgatagcttc aaacggtggt ggtcgtccgg
     6301 cactggaaag cattgttgca cagctgagcc gtccggatcc ggcactggca gcactgacca
     6361 atgatcatct ggttgcactg gcatgtctgg gtggtcgccc tgccctggat gcagttaaaa
     6421 aaggtctgcc gcatgcaccg gcactgatta aacgtaccaa tcgtcgtatt ccggaacgta
     6481 ccagccatcg tgttgcagat catgcacagg ttgttcgtgt tctgggtttt tttcagtgtc
     6541 atagccatcc ggcacaggca tttgatgatg caatgaccca gtttggtatg agcgattata
     6601 aagatgatga tgataaataa ggtaccctaa tcatacctga cctccatagc agaaagtcaa
     6661 aaacctccga ccggaggctt ttgacttgag ggggatccgc cc
//
