LOCUS       Exported                3704 bp ds-DNA     circular SYN 20-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p5mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 21, 2017 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     LOCUS pMSB-6 4169 bp DNA circular 11-OCT-2010 DEFINITION Cloning 
            vector pTrc99A, complete sequence. ACCESSION M22744 VERSION M22744.1
            GI:340740 KEYWORDS cloning vector. SOURCE Cloning vector pTrc99A. 
            ORGANISM Cloning vector pTrc99A other sequences; artificial 
            sequences; vectors. REFERENCE 1 (sites) AUTHORS Amann,E. and 
            Brosius,J. TITLE 'ATG vectors' for regulated high-level expression 
            of cloned genes in Escherichia coli JOURNAL Gene 40 (2-3), 183-190 
            (1985) REFERENCE 2 (bases 1 to 4176) AUTHORS Amann,E., Ochs,B. and 
            Abel,K.J. TITLE Tightly regulated tac promoter vectors useful for 
            the expression of unfused and fused proteins in Escherichia coli 
            JOURNAL Gene 69 (2), 301-315 (1988) COMMENT ApEinfo:methylated:0 
            COMMENT This file is created by Vector NTI 
            http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT 
            VNTDATE|486229983| COMMENT VNTDBDATE|567798251| COMMENT LSOWNER| 
            COMMENT VNTNAME|pMSB-6| COMMENT VNTAUTHORNAME|Demo User| COMMENT 
            VNTOAUTHORNAME|UNKNOWN| COMMENT 
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FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    176..212
                     /note="P102 promoter"
     misc_feature    176..181
                     /note="-35"
     misc_feature    199..204
                     /note="-10"
     protein_bind    211..231
                     /note="lac O1 operator"
     misc_feature    213..231
                     /note="TALElacO binding site"
     RBS             264..269
                     /note="RBS"
     gene            278..988
                     /note="mCherry"
     misc_feature    1027..1451
                     /note="5S RNA, T1, T2, rrnB"
     CDS             1544..2401
                     /note="AmpR"
     misc_feature    2559..3178
                     /note="colE1"
ORIGIN
        1 cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc
       61 tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat
      121 aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac
      181 agctagctca gtcctaggta ctgtgctagc aattgtgagc ggataacaat ttcgaattca
      241 aaagatctat cgatcgagga tccaggaggt acaatcaatg gtgagcaagg gcgaggagga
      301 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      361 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      421 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      481 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      541 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      601 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      661 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      721 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      781 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      841 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      901 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      961 cggcggcatg gacgagctgt acaagtaagg tacctctaga gtcgacctgc aggcatgcaa
     1021 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
     1081 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     1141 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     1201 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1261 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     1321 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     1381 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     1441 tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     1801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctacag caatggcaac
     2101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2701 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     3361 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     3421 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     3481 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3541 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3601 tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg
     3661 catgcattta cgttgcgaat tgatctggtt tgacagctta tcat
//
