LOCUS       Exported                3712 bp ds-DNA     circular SYN 21-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p5lysAsfGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 21, 2017 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     LOCUS pMSB-6 4169 bp DNA circular 11-OCT-2010 DEFINITION Cloning 
            vector pTrc99A, complete sequence. ACCESSION M22744 VERSION M22744.1
            GI:340740 KEYWORDS cloning vector. SOURCE Cloning vector pTrc99A. 
            ORGANISM Cloning vector pTrc99A other sequences; artificial 
            sequences; vectors. REFERENCE 1 (sites) AUTHORS Amann,E. and 
            Brosius,J. TITLE 'ATG vectors' for regulated high-level expression 
            of cloned genes in Escherichia coli JOURNAL Gene 40 (2-3), 183-190 
            (1985) REFERENCE 2 (bases 1 to 4176) AUTHORS Amann,E., Ochs,B. and 
            Abel,K.J. TITLE Tightly regulated tac promoter vectors useful for 
            the expression of unfused and fused proteins in Escherichia coli 
            JOURNAL Gene 69 (2), 301-315 (1988) COMMENT ApEinfo:methylated:0 
            COMMENT This file is created by Vector NTI 
            http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT 
            VNTDATE|486229983| COMMENT VNTDBDATE|567798251| COMMENT LSOWNER| 
            COMMENT VNTNAME|pMSB-6| COMMENT VNTAUTHORNAME|Demo User| COMMENT 
            VNTOAUTHORNAME|UNKNOWN| COMMENT 
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FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    176..210
                     /note="P102 promoter"
     misc_feature    176..181
                     /note="-35"
     misc_feature    199..204
                     /note="-10"
     misc_feature    211..233
                     /note="TlysA binding site"
     RBS             266..271
                     /note="RBS"
     gene            280..996
                     /note="sfGFP"
     misc_feature    1035..1459
                     /note="5S RNA, T1, T2, rrnB"
     CDS             1552..2409
                     /note="AmpR"
     misc_feature    2567..3186
                     /note="colE1"
ORIGIN
        1 cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc
       61 tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat
      121 aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac
      181 agctagctca gtcctaggta ctgtgctagc tctttttatg atgtggcgta atctcgaatt
      241 caaaagatct atcgatcgag gatccaggag gtacaatcaa tgagcaaagg agaagaactt
      301 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      361 tctgtccgtg gagagggtga aggtgatgct acaaacggaa aactcaccct taaatttatt
      421 tgcactactg gaaaactacc tgttccgtgg ccaacacttg tcactactct gacctatggt
      481 gttcaatgct tttcccgtta tccggatcac atgaaacggc atgacttttt caagagtgcc
      541 atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg gacctacaag
      601 acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaagggt
      661 attgatttta aagaagatgg aaacattctt ggacacaaac ttgagtacaa ctttaactca
      721 cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
      781 cgccacaacg ttgaagatgg ttccgttcaa ctagcagacc attatcaaca aaatactcca
      841 attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgtcctt
      901 tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct
      961 gggattacac atggcatgga tgagctctac aaataaggta cctctagagt cgacctgcag
     1021 gcatgcaagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
     1081 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
     1141 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
     1201 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
     1261 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1321 tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg
     1381 cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt
     1441 tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1501 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1561 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1621 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1681 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1741 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     1801 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1861 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1921 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1981 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2041 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca
     2101 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2161 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2221 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2281 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2341 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2401 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2461 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2521 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2701 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3181 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3241 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3301 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3361 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     3421 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     3481 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3541 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3601 agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg
     3661 cgaagcggca tgcatttacg ttgcgaattg atctggtttg acagcttatc at
//
