LOCUS       Exported                3701 bp ds-DNA     circular SYN 21-APR-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p5sucAmCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 21, 2017 from SnapGene 3.1.4
            http://www.snapgene.com
COMMENT     LOCUS pMSB-6 4169 bp DNA circular 11-OCT-2010 DEFINITION Cloning 
            vector pTrc99A, complete sequence. ACCESSION M22744 VERSION M22744.1
            GI:340740 KEYWORDS cloning vector. SOURCE Cloning vector pTrc99A. 
            ORGANISM Cloning vector pTrc99A other sequences; artificial 
            sequences; vectors. REFERENCE 1 (sites) AUTHORS Amann,E. and 
            Brosius,J. TITLE 'ATG vectors' for regulated high-level expression 
            of cloned genes in Escherichia coli JOURNAL Gene 40 (2-3), 183-190 
            (1985) REFERENCE 2 (bases 1 to 4176) AUTHORS Amann,E., Ochs,B. and 
            Abel,K.J. TITLE Tightly regulated tac promoter vectors useful for 
            the expression of unfused and fused proteins in Escherichia coli 
            JOURNAL Gene 69 (2), 301-315 (1988) COMMENT ApEinfo:methylated:0 
            COMMENT This file is created by Vector NTI 
            http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT 
            VNTDATE|486229983| COMMENT VNTDBDATE|567798251| COMMENT LSOWNER| 
            COMMENT VNTNAME|pMSB-6| COMMENT VNTAUTHORNAME|Demo User| COMMENT 
            VNTOAUTHORNAME|UNKNOWN| COMMENT 
            Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc
            ""pMSB-6"" 0 4169 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 """" """" 0 0 0 
            0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 """") COMMENT 
            (CDocSetData 1 1 0 0 0 2 ""MAIN"" 1 1 1 1 0 0 1 1 0 1 10 10 
            4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) 
            (CWordArray) COMMENT (CStringList ""AflII"" ""AflIII"" ""BamHI"" 
            ""BglI"" ""BglII"" ""ClaI"" ""EcoRI"" COMMENT ""HaeI"" ""HindIII"" 
            ""KpnI"" ""MfeI"" ""NcoI"" ""NdeI"" ""NheI"" ""NotI"" ""PstI"" 
            COMMENT ""PvuII"" ""SacI"" ""SalI"" ""SapI"" ""SpeI"" ""SphI"" 
            ""XbaI"" ""XhoI"" ""XmaI"") COMMENT (CStringList ""atg"" ""gtg"") 
            (CStringList ""taa"" ""tga"" ""tag"") (CObList) 1 COMMENT 
            ""{(0,1),2}"" 0 0 """" 0 4294967295 0 0 0 0 0 0 0 ""MAIN"" 0 0 30 0 
            COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
            COMMENT (CMolPar 0 0 0 0 0 1 4169 0 0 0 0 0 0 0 0) (CStringList) 
            (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar
            25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT 
            (CObList #0=(CRSite (CStringList) ""AflII"" ""cttaag"" 2 0 0 0 """")
            COMMENT #1=(CRSite (CStringList) ""AflIII"" ""acrygt"" 2 0 2 2253 0 
            3137 0 0 """") COMMENT #2=(CRSite (CStringList) ""BamHI"" ""ggatcc""
            2 0 1 14 0 0 """") COMMENT #3=(CRSite (CStringList) ""BglI"" 
            ""gccnnnnnggc"" 8 0 1 1247 0 0 """") COMMENT #4=(CRSite 
            (CStringList) ""BglII"" ""agatct"" 2 0 1 4168 0 0 """") COMMENT 
            #5=(CRSite (CStringList) ""ClaI"" ""atcgat"" 3 0 1 6 0 0 """") 
            COMMENT #6=(CRSite (CStringList) ""EcoRI"" ""gaattc"" 2 0 1 4159 0 0
            """") COMMENT #7=(CRSite (CStringList) ""HaeI"" ""wggccw"" 4 0 6 444
            0 460 0 1779 0 2231 0 COMMENT 2242 0 2865 0 0 """") COMMENT 
            #8=(CRSite (CStringList) ""HindIII"" ""aagctt"" 2 0 1 53 0 0 """") 
            COMMENT #9=(CRSite (CStringList) ""KpnI"" ""ggtacc"" 6 0 1 27 0 0 
            """") COMMENT #10=(CRSite (CStringList) ""MfeI"" ""caattg"" 2 0 0 0 
            """") COMMENT #11=(CRSite (CStringList) ""NcoI"" ""ccatgg"" 2 0 0 0 
            """") COMMENT #12=(CRSite (CStringList) ""NdeI"" ""catatg"" 3 0 1 
            2432 0 0 """") COMMENT #13=(CRSite (CStringList) ""NheI"" ""gctagc""
            2 0 0 0 """") COMMENT #14=(CRSite (CStringList) ""NotI"" 
            ""gcggccgc"" 3 0 0 0 """") COMMENT #15=(CRSite (CStringList) 
            ""PstI"" ""ctgcag"" 6 0 1 45 0 0 """") COMMENT #16=(CRSite 
            (CStringList) ""PvuII"" ""cagctg"" 4 0 2 3737 0 3830 0 0 """") 
            COMMENT #17=(CRSite (CStringList) ""SacI"" ""gagctc"" 6 0 0 0 """") 
            COMMENT #18=(CRSite (CStringList) ""SalI"" ""gtcgac"" 2 0 1 35 0 0 
            """") COMMENT #19=(CRSite (CStringList) ""SapI"" ""gctcttc"" 9 12 1 
            2370 1 0 """") COMMENT #20=(CRSite (CStringList) ""SpeI"" ""actagt""
            2 0 0 0 """") COMMENT #21=(CRSite (CStringList) ""SphI"" ""gcatgc"" 
            6 0 2 51 0 2698 0 0 """") COMMENT #22=(CRSite (CStringList) ""XbaI""
            ""tctaga"" 2 0 1 29 0 0 """") COMMENT #23=(CRSite (CStringList) 
            ""XhoI"" ""ctcgag"" 2 0 0 0 """") COMMENT #24=(CRSite (CStringList) 
            ""XmaI"" ""cccggg"" 2 0 0 0 """")) COMMENT (CObList COMMENT 
            #25=(CFSignal (CObList) ""trc promoter"" 30 0 0 3925 4157 0 
            (CStringList) COMMENT (CStringList ""/dnas_title=\""trc 
            promoter\"""" ""/citation=[1]"" COMMENT ""/ApEinfo_fwdcolor=cyan"" 
            ""/ApEinfo_revcolor=green"") 1 1 1 1 COMMENT ""1496..1728"") COMMENT
            #26=(CFSignal (CObList) ""5S RNA, T1, T2, rrnB"" 21 0 0 60 484 0 
            COMMENT (CStringList) COMMENT (CStringList ""/dnas_title=\""5S RNA, 
            T1, T2, rrnB\"""" COMMENT ""/ApEinfo_fwdcolor=cyan"" 
            ""/ApEinfo_revcolor=green"") 1 1 1 1 COMMENT ""1800..2224"") COMMENT
            #27=(CFSignal (CObList) ""lacI-q region"" 21 0 0 2708 3894 0 
            (CStringList) COMMENT (CStringList ""/dnas_title=\""lacI-q 
            region\"""" COMMENT ""/ApEinfo_fwdcolor=cyan"" 
            ""/ApEinfo_revcolor=green"") 1 1 1 1 COMMENT ""279..1465"") COMMENT 
            #28=(CFSignal (CObList) ""AmpR"" 4 0 0 577 1434 0 (CStringList) 
            COMMENT (CStringList ""/dnas_title=\""AmpR\"""") 1 1 1 1 
            ""2317..3174"") COMMENT #29=(CFSignal (CObList) ""MSB6-Fwd"" 28 0 0 
            4158 13 0 (CStringList) COMMENT (CStringList 
            ""/dnas_title=\""MSB6-Fwd\"""") 1 1 1 1 ""1729..1753"") COMMENT 
            #30=(CFSignal (CObList) ""MSB6-Rev"" 28 0 0 4133 4157 0 
            (CStringList) COMMENT (CStringList ""/dnas_title=\""MSB6-Rev\"""") 1
            1 1 1 ""1704..1728"") COMMENT #31=(CFSignal (CObList) ""trc_seq"" 28
            0 0 4005 4025 0 (CStringList) COMMENT (CStringList 
            ""/dnas_title=\""trc_seq\"""") 1 1 1 1 ""1576..1596"") COMMENT 
            #32=(CFSignal (CObList) ""rrnB_seq"" 28 0 1 69 89 0 (CStringList) 
            COMMENT (CStringList ""/dnas_title=\""rrnB_seq\"""") 1 1 1 1 COMMENT
            ""complement(1809..1829)"") COMMENT #33=(CFSignal (CObList) 
            ""lacIq"" 4 0 0 2777 3865 0 (CStringList) COMMENT (CStringList 
            ""/dnas_title=\""lacIq\"""") 1 1 1 1 ""348..1436"") COMMENT 
            #34=(CFSignal (CObList) ""colE1"" 21 0 0 1592 2211 0 (CStringList) 
            COMMENT (CStringList) 1 1 1 1 """")) (CObList) (CObList) (CObList) 
            (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT 
            #35=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList
            COMMENT #36=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0
            1 233) ""pMSB-6"" 2) 5 """" 0 COMMENT 4) COMMENT #37=(CFolderPar 
            COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT 
            (CObjectList COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
            COMMENT ""DNA 'pMSB-6'"" 1) COMMENT #39=(CLinePar (CParagraph 0 (0 
            0) 1 2 1 0 180) COMMENT ""Cloning vector pTrc99A, complete 
            sequence."" 1) COMMENT #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 
            180) COMMENT ""Currently local object. Original author: UNKNOWN"" 1)
            COMMENT #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT 
            ""Created: 06/27/08 03:53PM"" 1) COMMENT #42=(CLinePar (CParagraph 0
            (0 0) 1 2 1 0 180) COMMENT ""Last Modified: 10/11/10 05:44PM"" 1) 
            COMMENT #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT 
            ""length: 4169 bp"" 1) COMMENT #44=(CLinePar (CParagraph 0 (0 0) 1 2
            1 0 180) COMMENT ""storage type: Basic"" 1) COMMENT #45=(CLinePar 
            (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""form: Circular"" 1))) 
            ""General Description"") COMMENT #46=(CFolderPar COMMENT (CGroupPar 
            (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT 
            #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Keywords: 
            cloning vector"" 1) COMMENT #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 
            0 180) COMMENT ""Original Source Database: GenBank"" 1) COMMENT 
            #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT 
            ""Modification Date in the Original DB: 10-OCT-2008"" 1) COMMENT 
            #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Original 
            Accession Number(s): <a href=\""ncbilink:dnarna/M22744\"">M22744"" 
            COMMENT 1) COMMENT #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
            COMMENT ""Version in the Original DB: M22744.1 GI:340740"" 1) 
            COMMENT #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT 
            ""Sequence Source: Cloning vector pTrc99A."" 1) COMMENT 
            #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT 
            ""Organizm(s): <a 
            href=\""ncbilink:taxonomy/Cloning+vector+pTrc99A+other+sequences;+ar
            tificial+sequences;\"">Cloning vector pTrc99A other sequences; 
            artificial sequences;(Cloning vector pTrc99A other sequences; 
            artificial sequences;)"" COMMENT 1) COMMENT #54=(CLinePar 
            (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Taxonomy: vectors"" 1))) 
            ""Standard Fields"") COMMENT #55=(CFolderPar COMMENT (CGroupPar 
            (CParagraph 3 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT 
            #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""REFERENCE 1
            (sites)"" 1) COMMENT #57=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
            COMMENT "" AUTHORS Amann,E. and Brosius,J."" 1) COMMENT 
            #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "" TITLE 'ATG
            vectors' for regulated high-level expression of cloned genes"" 
            COMMENT 1) COMMENT #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
            COMMENT "" in Escherichia coli"" 1) COMMENT #60=(CLinePar 
            (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "" JOURNAL Gene 40 (2-3), 
            183-190 (1985)"" 1) COMMENT #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 
            0 180) COMMENT ""REFERENCE 2 (bases 1 to 4176)"" 1) COMMENT 
            #62=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "" AUTHORS 
            Amann,E., Ochs,B. and Abel,K.J."" 1) COMMENT #63=(CLinePar 
            (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "" TITLE Tightly regulated 
            tac promoter vectors useful for the expression of"" COMMENT 1) 
            COMMENT #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "" 
            unfused and fused proteins in Escherichia coli"" COMMENT 1) COMMENT 
            #65=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "" JOURNAL 
            Gene 69 (2), 301-315 (1988)"" 1))) COMMENT ""References"") COMMENT 
            #66=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
            ""Comments"" 2) 1 """" COMMENT 0 0) COMMENT #67=(CFolderPar COMMENT 
            (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT 
            ""Annotations"") COMMENT #68=(CFolderPar COMMENT (CGroupPar 
            (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT 
            #69=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 
            178) COMMENT (CObjectList COMMENT #70=(CFolderPar COMMENT (CGroupPar
            COMMENT (CParagraph 577 (3 #28# 0) 1 2 2 0 327) COMMENT (CObjectList
            COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Start: 577 End: 1434"" 1) COMMENT #72=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Original Location 
            Description:"" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 
            1 2 3 0 180) COMMENT "" 2317..3174"" 1) COMMENT #74=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Qualifiers:"" 1) 
            COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT "" /dnas_title=\""AmpR\"""" 1))) ""AmpR"") COMMENT 
            #76=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2777 (3 #33# 
            0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #77=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 2777 End: 3865"" 
            1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Original Location Description:"" 1) COMMENT #79=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 348..1436"" 1) 
            COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Qualifiers:"" 1) COMMENT #81=(CLinePar COMMENT (CParagraph
            0 (0 0) 1 2 3 0 180) COMMENT "" /dnas_title=\""lacIq\"""" 1))) 
            ""lacIq""))) COMMENT ""CDS (2 total)"") COMMENT #82=(CFolderPar 
            COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT 
            (CObjectList COMMENT #83=(CFolderPar COMMENT (CGroupPar COMMENT 
            (CParagraph 60 (3 #26# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT 
            #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT 
            ""Start: 60 End: 484 "" 1) COMMENT #85=(CLinePar COMMENT (CParagraph
            0 (0 0) 1 2 3 0 180) COMMENT ""5S RNA, T1, T2, rrnB"" 1) COMMENT 
            #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT 
            ""Original Location Description:"" 1) COMMENT #87=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 1800..2224"" 1) COMMENT 
            #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT 
            ""Qualifiers:"" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0)
            1 2 3 0 180) COMMENT "" /dnas_title=\""5S RNA, T1, T2, rrnB\"""" 
            COMMENT 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0
            180) COMMENT "" /ApEinfo_fwdcolor=cyan"" 1) COMMENT #91=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 
            /ApEinfo_revcolor=green"" 1))) COMMENT ""5S RNA, T1, T2, rrnB"") 
            COMMENT #92=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1592 
            (3 #34# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #93=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1592 End: 
            2211"" 1))) ""colE1"") COMMENT #94=(CFolderPar COMMENT (CGroupPar 
            COMMENT (CParagraph 2708 (3 #27# 0) 1 2 2 0 194) COMMENT 
            (CObjectList COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3
            0 180) COMMENT ""Start: 2708 End: 3894"" 1) COMMENT #96=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""lacI-q region"" 
            1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Original Location Description:"" 1) COMMENT #98=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 279..1465"" 1) 
            COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Qualifiers:"" 1) COMMENT #100=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" /dnas_title=\""lacI-q 
            region\"""" 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 
            2 3 0 180) COMMENT "" /ApEinfo_fwdcolor=cyan"" 1) COMMENT 
            #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 
            /ApEinfo_revcolor=green"" 1))) COMMENT ""lacI-q region""))) ""Misc. 
            Feature (3 total)"") COMMENT #103=(CFolderPar COMMENT (CGroupPar 
            (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT 
            #104=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 69 (3 #32# 
            0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #105=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 69 End: 89 
            (Complementary)"" COMMENT 1) COMMENT #106=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Original Location 
            Description:"" 1) COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0)
            1 2 3 0 180) COMMENT "" complement(1809..1829)"" 1) COMMENT 
            #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT 
            ""Qualifiers:"" 1) COMMENT #109=(CLinePar COMMENT (CParagraph 0 (0 
            0) 1 2 3 0 180) COMMENT "" /dnas_title=\""rrnB_seq\"""" 1))) COMMENT
            ""rrnB_seq"") COMMENT #110=(CFolderPar COMMENT (CGroupPar COMMENT 
            (CParagraph 4005 (3 #31# 0) 1 2 2 0 194) COMMENT (CObjectList 
            COMMENT #111=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Start: 4005 End: 4025"" 1) COMMENT #112=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Original Location 
            Description:"" 1) COMMENT #113=(CLinePar COMMENT (CParagraph 0 (0 0)
            1 2 3 0 180) COMMENT "" 1576..1596"" 1) COMMENT #114=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Qualifiers:"" 1) 
            COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT "" /dnas_title=\""trc_seq\"""" 1))) COMMENT ""trc_seq"") 
            COMMENT #116=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4133
            (3 #30# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #117=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 4133 End: 
            4157"" 1) COMMENT #118=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0
            180) COMMENT ""Original Location Description:"" 1) COMMENT 
            #119=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 
            1704..1728"" 1) COMMENT #120=(CLinePar COMMENT (CParagraph 0 (0 0) 1
            2 3 0 180) COMMENT ""Qualifiers:"" 1) COMMENT #121=(CLinePar COMMENT
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 
            /dnas_title=\""MSB6-Rev\"""" 1))) COMMENT ""MSB6-Rev"") COMMENT 
            #122=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4158 (3 #29#
            0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #123=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 4158 End: 13 "" 1)
            COMMENT #124=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Original Location Description:"" 1) COMMENT #125=(CLinePar
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 1729..1753"" 1) 
            COMMENT #126=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT ""Qualifiers:"" 1) COMMENT #127=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 
            /dnas_title=\""MSB6-Fwd\"""" 1))) COMMENT ""MSB6-Fwd""))) ""Primer 
            Binding Site (4 total)"") COMMENT #128=(CFolderPar COMMENT 
            (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList
            COMMENT #129=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3925
            (3 #25# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #130=(CLinePar 
            COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 3925 End: 
            4157"" 1) COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0
            180) COMMENT ""trc promoter from pKK233-2"" 1) COMMENT 
            #132=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT 
            ""Original Location Description:"" 1) COMMENT #133=(CLinePar COMMENT
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" 1496..1728"" 1) COMMENT 
            #134=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT 
            ""Qualifiers:"" 1) COMMENT #135=(CLinePar COMMENT (CParagraph 0 (0 
            0) 1 2 3 0 180) COMMENT "" /dnas_title=\""trc promoter\"""" 1) 
            COMMENT #136=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT "" /citation=[1]"" 1) COMMENT #137=(CLinePar COMMENT 
            (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "" /ApEinfo_fwdcolor=cyan""
            1) COMMENT #138=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) 
            COMMENT "" /ApEinfo_revcolor=green"" 1))) COMMENT ""trc 
            promoter""))) COMMENT ""Promoter Prokaryotic (1 total)""))) 
            ""Feature Map"") COMMENT #139=(CFolderPar COMMENT (CGroupPar 
            (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT 
            #140=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31384816 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) 
            ""AflII: 0 sites"") 1 5 ""CTTAAG"" COMMENT ""GAATTC"") COMMENT 
            #141=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31383504 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #142=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #143=(CLinePar COMMENT (CParagraph 0 (1
            #1# 1) 1 2 2 0 191) COMMENT "" N1: 2253 "" 1) COMMENT #144=(CLinePar
            COMMENT (CParagraph 0 (1 #1# 2) 1 2 2 0 191) COMMENT "" N2: 3137 "" 
            1))))) COMMENT ""AflIII: 2 sites"") 1 5 ""ACRYGT"" ""TGYRCA"") 
            COMMENT #145=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31383472 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #146=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #147=(CLinePar COMMENT (CParagraph 0 (1
            #2# 1) 1 2 2 0 191) COMMENT "" N1: 14 "" 1))))) ""BamHI: 1 site"") 1
            COMMENT 5 ""GGATCC"" ""CCTAGG"") COMMENT #148=(CRSFolderPar COMMENT 
            (CFolderPar COMMENT (CGroupPar (CParagraph 31386256 (8 0) 1 1 1 0 
            178) COMMENT (CObjectList COMMENT #149=(CGroupPar COMMENT 
            (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT 
            #150=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT 
            "" N1: 1247 "" 1))))) ""BglI: 1 site"") COMMENT 7 4 ""GCCNNNNNGGC"" 
            ""CGGNNNNNCCG"") COMMENT #151=(CRSFolderPar COMMENT (CFolderPar 
            COMMENT (CGroupPar (CParagraph 31383216 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList COMMENT #152=(CGroupPar COMMENT (CParagraph 0 (10 #4# 
            0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #153=(CLinePar COMMENT 
            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT "" N1: 4168 "" 1))))) 
            ""BglII: 1 site"") COMMENT 1 5 ""AGATCT"" ""TCTAGA"") COMMENT 
            #154=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31383408 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #155=(CGroupPar COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #156=(CLinePar COMMENT (CParagraph 0 (1
            #5# 1) 1 2 2 0 191) COMMENT "" N1: 6 "" 1))))) ""ClaI: 1 site"") 2 4
            COMMENT ""ATCGAT"" ""TAGCTA"") COMMENT #157=(CRSFolderPar COMMENT 
            (CFolderPar COMMENT (CGroupPar (CParagraph 31383440 (8 0) 1 1 1 0 
            178) COMMENT (CObjectList COMMENT #158=(CGroupPar COMMENT 
            (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT 
            #159=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT 
            "" N1: 4159 "" 1))))) ""EcoRI: 1 site"") COMMENT 1 5 ""GAATTC"" 
            ""CTTAAG"") COMMENT #160=(CRSFolderPar COMMENT (CFolderPar COMMENT 
            (CGroupPar (CParagraph 31383376 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList)) ""HaeI: 6 sites"") 3 3 ""WGGCCW"" COMMENT ""WCCGGW"")
            COMMENT #161=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31383312 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #162=(CGroupPar COMMENT (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #163=(CLinePar COMMENT (CParagraph 0 (1
            #8# 1) 1 2 2 0 191) COMMENT "" N1: 53 "" 1))))) ""HindIII: 1 site"")
            COMMENT 1 5 ""AAGCTT"" ""TTCGAA"") COMMENT #164=(CRSFolderPar 
            COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 31381808 (8 0) 1 
            1 1 0 178) COMMENT (CObjectList COMMENT #165=(CGroupPar COMMENT 
            (CParagraph 0 (10 #9# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT 
            #166=(CLinePar COMMENT (CParagraph 0 (1 #9# 1) 1 2 2 0 191) COMMENT 
            "" N1: 27 "" 1))))) ""KpnI: 1 site"") 5 COMMENT 1 ""GGTACC"" 
            ""CCATGG"") COMMENT #167=(CRSFolderPar COMMENT (CFolderPar COMMENT 
            (CGroupPar (CParagraph 31384624 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList)) ""MfeI: 0 sites"") 1 5 ""CAATTG"" COMMENT ""GTTAAC"")
            COMMENT #168=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31384560 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) 
            ""NcoI: 0 sites"") 1 5 ""CCATGG"" COMMENT ""GGTACC"") COMMENT 
            #169=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 31384784 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #170=(CGroupPar COMMENT (CParagraph 0 (10 #12# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #171=(CLinePar COMMENT (CParagraph 0 (1
            #12# 1) 1 2 2 0 191) COMMENT "" N1: 2432 "" 1))))) ""NdeI: 1 site"")
            COMMENT 2 4 ""CATATG"" ""GTATAC"") COMMENT #172=(CRSFolderPar 
            COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 31384752 (8 0) 1 
            1 1 0 178) COMMENT (CObjectList)) ""NheI: 0 sites"") 1 5 ""GCTAGC"" 
            COMMENT ""CGATCG"") COMMENT #173=(CRSFolderPar COMMENT (CFolderPar 
            COMMENT (CGroupPar (CParagraph 31384528 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList)) ""NotI: 0 sites"") 2 6 ""GCGGCCGC"" COMMENT 
            ""CGCCGGCG"") COMMENT #174=(CRSFolderPar COMMENT (CFolderPar COMMENT
            (CGroupPar (CParagraph 71888008 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList COMMENT #175=(CGroupPar COMMENT (CParagraph 0 (10 #15# 
            0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #176=(CLinePar COMMENT 
            (CParagraph 0 (1 #15# 1) 1 2 2 0 191) COMMENT "" N1: 45 "" 1))))) 
            ""PstI: 1 site"") 5 COMMENT 1 ""CTGCAG"" ""GACGTC"") COMMENT 
            #177=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 71888040 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #178=(CGroupPar COMMENT (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #179=(CLinePar COMMENT (CParagraph 0 (1
            #16# 1) 1 2 2 0 191) COMMENT "" N1: 3737 "" 1) COMMENT 
            #180=(CLinePar COMMENT (CParagraph 0 (1 #16# 2) 1 2 2 0 191) COMMENT
            "" N2: 3830 "" 1))))) ""PvuII: 2 sites"") COMMENT 3 3 ""CAGCTG"" 
            ""GTCGAC"") COMMENT #181=(CRSFolderPar COMMENT (CFolderPar COMMENT 
            (CGroupPar (CParagraph 71888072 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList)) ""SacI: 0 sites"") 5 1 ""GAGCTC"" COMMENT ""CTCGAG"")
            COMMENT #182=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 71888104 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #183=(CGroupPar COMMENT (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #184=(CLinePar COMMENT (CParagraph 0 (1
            #18# 1) 1 2 2 0 191) COMMENT "" N1: 35 "" 1))))) ""SalI: 1 site"") 1
            COMMENT 5 ""GTCGAC"" ""CAGCTG"") COMMENT #185=(CRSFolderPar COMMENT 
            (CFolderPar COMMENT (CGroupPar (CParagraph 71888136 (8 0) 1 1 1 0 
            178) COMMENT (CObjectList COMMENT #186=(CGroupPar COMMENT 
            (CParagraph 0 (10 #19# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT 
            #187=(CLinePar COMMENT (CParagraph 0 (1 #19# 1) 1 2 2 0 191) COMMENT
            "" N1: 2370 (on complementary strand)"" COMMENT 1))))) ""SapI: 1 
            site"") 8 11 COMMENT ""GCTCTTCNNNNN"" ""CGAGAAGNNNNN"") COMMENT 
            #188=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 71888168 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) 
            ""SpeI: 0 sites"") 1 5 ""ACTAGT"" COMMENT ""TGATCA"") COMMENT 
            #189=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 71888200 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #190=(CGroupPar COMMENT (CParagraph 0 (10 #21# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #191=(CLinePar COMMENT (CParagraph 0 (1
            #21# 1) 1 2 2 0 191) COMMENT "" N1: 51 "" 1) COMMENT #192=(CLinePar 
            COMMENT (CParagraph 0 (1 #21# 2) 1 2 2 0 191) COMMENT "" N2: 2698 ""
            1))))) ""SphI: 2 sites"") COMMENT 5 1 ""GCATGC"" ""CGTACG"") COMMENT
            #193=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar 
            (CParagraph 71888232 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT
            #194=(CGroupPar COMMENT (CParagraph 0 (10 #22# 0) 1 2 2 0 180) 
            COMMENT (CObjectList COMMENT #195=(CLinePar COMMENT (CParagraph 0 (1
            #22# 1) 1 2 2 0 191) COMMENT "" N1: 29 "" 1))))) ""XbaI: 1 site"") 1
            COMMENT 5 ""TCTAGA"" ""AGATCT"") COMMENT #196=(CRSFolderPar COMMENT 
            (CFolderPar COMMENT (CGroupPar (CParagraph 71888264 (8 0) 1 1 1 0 
            178) COMMENT (CObjectList)) ""XhoI: 0 sites"") 1 5 ""CTCGAG"" 
            COMMENT ""GAGCTC"") COMMENT #197=(CRSFolderPar COMMENT (CFolderPar 
            COMMENT (CGroupPar (CParagraph 71888296 (8 0) 1 1 1 0 178) COMMENT 
            (CObjectList)) ""XmaI: 0 sites"") 1 5 ""CCCGGG"" COMMENT 
            ""GGGCCC""))) COMMENT ""Restriction/Methylation Map: (no more than 2
            sites)"")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT 
            (CObjectList COMMENT #198=(CWidgetStyle ""RSite Label"" 1 (LOGPEN 0 
            0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 
            ""Georgia"") 0.555556 0 1 5 COMMENT ""@N (@S)"" 0) COMMENT 
            #199=(CWidgetStyle ""Signal Label"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT 
            (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 ""Arial"") 0.666667 0 1 1 
            COMMENT ""@N"" 0) COMMENT #200=(CWidgetStyle ""Molecule Label 2"" 0 
            0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 
            0.555556 0 1 16 COMMENT ""@L bp"" 0) COMMENT #201=(CWidgetStyle 
            ""Molecule Label 1"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 
            1 34 ""Verdana"") 0.833333 0 1 1 COMMENT ""@N"" 0) COMMENT 
            #202=(CWidgetStyle ""Shape 3"" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT 
            (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT 
            #203=(CWidgetStyle ""Shape 1"" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT 
            (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT 
            #204=(CWidgetStyle ""Axis"" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT 
            (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT 
            #205=(CWidgetStyle ""Line 2"" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
            COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #206=(CWidgetStyle ""RSite""
            1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) 
            COMMENT #207=(CWidgetStyle ""Short Signal"" 1 (LOGPEN 0 0 13395507) 
            10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT 
            #208=(CWidgetStyle ""Uniq RSite Label"" 1 (LOGPEN 0 0 153) 1 0 1 
            COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 
            128 1 COMMENT 5 ""@N (@S)"" 0) COMMENT #209=(CWidgetStyle 
            ""Vanilla"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 
            COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 ""Times New Roman"") 
            0.8 0 COMMENT 1 2 ""?"" 0) COMMENT #210=(CWidgetStyle ""Mark 1"" 0 0
            1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 ""Windings"") 0.7 0 
            1 2 ""?"" COMMENT 0) COMMENT #211=(CWidgetStyle ""Motif Label"" 1 
            (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2
            1 34 ""Arial"") 0.611111 8388608 COMMENT 1 65535 ""@N (@H)"" 0) 
            COMMENT #212=(CWidgetStyle ""Fragment Label 2"" 1 (LOGPEN 0 0 0) 1 0
            1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 ""Courier New"") 
            1.05 0 1 48 COMMENT ""@F bp (molecule @L bp)"" 0) COMMENT 
            #213=(CWidgetStyle ""Fragment Label 1"" 1 (LOGPEN 0 0 0) 1 0 1 
            COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.91 0 1 1 
            COMMENT ""Fragment of @N"" 0) COMMENT #214=(CWidgetStyle ""Shape 4""
            1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT 
            #215=(CWidgetStyle ""Shape 2"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 
            0) 0 COMMENT 0 0) COMMENT #216=(CWidgetStyle ""Shape 0"" 1 (LOGPEN 0
            0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #217=(CWidgetStyle 
            ""ORF"" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 
            3.41182 2.86186 0.609808)) COMMENT #218=(CWidgetStyle ""Line 4"" 1 
            (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #219=(CWidgetStyle ""Line 3"" 
            1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #220=(CWidgetStyle ""Line
            1"" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #221=(CWidgetStyle 
            ""Short Promoter"" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT 
            #222=(CWidgetStyle ""Motif"" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT 
            #223=(CWidgetStyle ""Line 0"" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT 
            #224=(CWidgetStyle ""Void"" 0 0 0 0 0) COMMENT #225=(CWidgetStyle 
            ""General Label"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 
            400 0 0 0 0 3 2 1 18 ""Times New Roman"") 0.91 0 COMMENT 1 3 ""@T @N
            "" 0) COMMENT #226=(CWidgetStyle ""Position"" 1 (LOGPEN 0 0 0) 1 0 0
            0 0) COMMENT #227=(CWidgetStyle ""Annotation"" 0 0 1 COMMENT 
            (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") 0.91 0 
            COMMENT 0 0) COMMENT #228=(CWidgetStyle ""Position Label"" 1 (LOGPEN
            0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 
            ""Arial"") 0.63 8388608 1 1 COMMENT ""@N"" 0) COMMENT 
            #229=(CWidgetStyle ""Range"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 
            0 16777215 0) 0 0 0) COMMENT #230=(CWidgetStyle ""Range Label"" 1 
            (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 
            1 34 ""Arial"") 0.63 8388608 1 1 COMMENT ""@N"" 0) COMMENT 
            #231=(CWidgetStyle ""ORF Label"" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT 
            (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") COMMENT 
            0.611111 0 1 65535 ""@N"" 0) COMMENT #232=(CWidgetStyle ""CDS 
            Label"" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 
            0 0 0 3 2 1 34 ""Arial"") 0.555556 255 1 1 COMMENT ""@N"" 0) COMMENT
            #233=(CWidgetStyle ""Shape 5"" 1 (LOGPEN 0 0 0) 3 1 COMMENT 
            (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 
            2 50 ""Arial"") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT 
            #234=(CWidgetStyle ""CDS"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3)
            0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #235=(CWidgetStyle 
            ""Label 2"" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 
            400 0 0 0 0 3 2 1 34 ""Arial"") 0.944444 8388608 COMMENT 1 1 ""@N"" 
            0) COMMENT #236=(CWidgetStyle ""Label 3"" 1 (LOGPEN 0 0 8421376) 1 0
            1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 ""Arial"") 
            0.833333 255 1 COMMENT 5 ""@N (@S)"" 0) COMMENT #237=(CWidgetStyle 
            ""Label 4"" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 
            400 0 0 0 0 3 2 1 34 ""Arial"") 0.722222 0 1 5 COMMENT ""@N (@S)"" 
            0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 
            ""Unique RSite"" 1 ""Uniq RSite Label"") 67 COMMENT (CShapeMapEntry 
            0 ""ORF"" 0 ""ORF Label"")) 77.1989 77.1989 85.7783 COMMENT 85.7783 
            0.1 -4169) 1 1 0 1 1 COMMENT (mapper: 17.9965 -20.5613 77.1989 
            77.1989 0.01 10 -1 4169 4169 1 0 0) COMMENT #238=(CGroupWidget 
            (CWidget 0 (0 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT 
            #239=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -822 100) COMMENT 
            (CObjectList COMMENT #240=(CAxis COMMENT (CWideLine COMMENT (CWidget
            0 (0 0) 1 2 0 0 #204# 71796780 0) COMMENT (LOGPEN 0 2 10079436) 2 
            (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27569 6.27369 1 0.0214037) 
            0.150472) COMMENT #241=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 
            #201# 1852270931 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 111 
            42 0 0 400 0 0 0 0 3 2 1 34 ""Verdana"") COMMENT 1.74233 0.833333 0 
            ""pMSB-6"" ""@N"" 1 0 0.871165 0 -10 COMMENT 4.14514 1.41194 Nil) 
            COMMENT #242=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #200# 0 100) 
            COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 
            1 18 ""Georgia"") COMMENT 1.74233 0.555556 0 ""4169 bp"" ""@L bp"" 
            16 0 -0.871165 COMMENT 0 -10.8 2.91455 0.958563 Nil)) (CObjectList))
            COMMENT #243=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1225 100) 
            COMMENT (CObjectList COMMENT #244=(CGroupWidget COMMENT (CWidget 4 
            (7 4 0) 1 2 0 0 Nil -553 100) COMMENT (CObjectList COMMENT 
            #245=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #28# 0) 1 
            2 0 0 #202# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 
            0 6724095 0) 1 4.11541 5.40647 1 COMMENT 0.082322) 0.8 1.8 0) 
            COMMENT #246=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #199# 10703 
            100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 700 0 0 0 0
            3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""AmpR"" ""@N"" 1 
            COMMENT 13.3173 0 0 -10 2.52594 1.10105 #245#) COMMENT 
            #247=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #33# 0) 1 
            2 0 0 #202# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 
            0 6724095 0) 1 0.457435 2.09608 1 COMMENT 0.082322) 0.8 1.8 0) 
            COMMENT #248=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #199# 38836800
            100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 700 0 0 0 0
            3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""lacIq"" ""@N"" 1 
            COMMENT -12.622 3.48466 0 -10 1.98189 1.10105 #247#)) COMMENT 
            (CObjectList)) COMMENT #249=(CGroupWidget COMMENT (CWidget 21 (7 21 
            0) 1 2 0 0 Nil -687 100) COMMENT (CObjectList COMMENT 
            #250=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #26# 0) 1 
            2 0 0 #203# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 
            0 10079334 0) 1 5.5449 6.18441 1 COMMENT 0.082322) 0.8 1.8 0) 
            COMMENT #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #199# 0 100) COMMENT 
            (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 700 0 0 0 0 3 2 1 34 
            COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""5S RNA, T1, T2, 
            rrnB"" ""@N"" 1 15.9108 3.48466 COMMENT 0 -10 8.14778 1.10105 #250#)
            COMMENT #252=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 
            #27# 0) 1 2 0 0 #203# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT 
            (LOGBRUSH 0 10079334 0) 0.835356 0.413798 COMMENT 2.1999 1 0.082322)
            0.8 1.8 0) COMMENT #253=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 
            #199# 1005454657 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 
            33 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 
            ""lacI-q region"" COMMENT ""@N"" 1 -14.6002 1.74233 0 -10 4.98712 
            1.10105 COMMENT #252#) COMMENT #254=(CWideArrow COMMENT (CWideLine 
            COMMENT (CWidget 0 (3 #34# 0) 1 2 0 0 #203# 0 100) COMMENT (LOGPEN 0
            0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.94625 3.87917 1 
            COMMENT 0.082322) 0.8 1.8 0) COMMENT #255=(CLabel (CWidget 0 (0 0) 1
            2 0 0 #199# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0
            0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 
            ""colE1"" ""@N"" 1 COMMENT 5.20147 -12.1963 0 -10 2.29278 1.10105 
            #254#)) COMMENT (CObjectList)) COMMENT #256=(CGroupWidget COMMENT 
            (CWidget 28 (7 28 0) 1 2 0 0 Nil -2570 100) COMMENT (CObjectList 
            COMMENT #257=(CScratch COMMENT (CWidget 0 (3 #29# 0) 1 2 0 0 #220# 0
            100) COMMENT (LOGPEN 0 0 16711680) 1 1 0.0180567 1.9 COMMENT 
            0.082322 1) COMMENT #258=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 
            #199# 574365534 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33
            0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 
            ""MSB6-Fwd"" ""@N"" COMMENT 1 -3.40647 13.9386 0 -10 4.31353 1.10105
            COMMENT #257#) COMMENT #259=(CScratch COMMENT (CWidget 0 (3 #30# 0) 
            1 2 0 0 #220# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 1 0.0556747 1.9
            COMMENT 0.082322 1) COMMENT #260=(CLabel (CWidget 0 (0 0) 1 2 0 0 
            #199# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 700
            0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""MSB6-Rev"" 
            ""@N"" COMMENT 1 -3.6451 12.1963 0 -10 4.17104 1.10105 #259#) 
            COMMENT #261=(CScratch COMMENT (CWidget 0 (3 #31# 0) 1 2 0 0 #220# 0
            100) COMMENT (LOGPEN 0 0 16711680) 1 1 0.248279 1.9 COMMENT 0.082322
            1) COMMENT #262=(CLabel (CWidget 0 (0 0) 1 2 0 0 #199# 0 100) 
            COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 700 0 0 0 0 3 2 
            1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""trc seq"" ""@N"" 1 
            COMMENT -10.235 8.71165 0 -10 2.79797 1.10105 #261#) COMMENT 
            #263=(CScratch COMMENT (CWidget 0 (3 #32# 0) 1 2 0 0 #220# 0 100) 
            COMMENT (LOGPEN 0 0 16711680) 1 1 6.17086 1.9 COMMENT 0.082322 1) 
            COMMENT #264=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #199# 
            2082603440 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 
            700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""rrnB 
            seq"" ""@N"" COMMENT 1 12.8821 5.22699 0 -10 3.48451 1.10105 #263#))
            COMMENT (CObjectList)) COMMENT #265=(CGroupWidget COMMENT (CWidget 
            30 (7 30 0) 1 2 0 0 Nil -956 100) COMMENT (CObjectList COMMENT 
            #266=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #25# 0) 1 
            2 0 0 #215# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 
            0.835356 COMMENT 0.0180567 0.368657 1 0.082322) 0.8 1.8 0) COMMENT 
            #267=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #199# 2082617568 100) 
            COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 89 33 0 0 700 0 0 0 0 3 2 
            1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""trc promoter"" COMMENT 
            ""@N"" 1 -9.2351 10.454 0 -10 4.98712 1.10105 COMMENT #266#)) 
            (CObjectList))) (CObjectList)) COMMENT #268=(CGroupWidget (CWidget 
            11 (8 0) 1 2 0 0 Nil -1494 100) COMMENT (CObjectList COMMENT 
            #269=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1360 
            100) COMMENT (CObjectList COMMENT #270=(CScratch COMMENT (CWidget 1 
            (1 #2# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 
            6.25362 1.9 COMMENT 0.082322 1) COMMENT #271=(CLabel (CWidget 0 (0 
            0) 1 2 0 0 #208# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 
            74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556
            128 COMMENT ""{\\i Bam}HI (14)"" ""@N (@S)"" 5 3.69191 COMMENT 
            17.4233 0 -10 4.5467 0.958563 #270#)) COMMENT (CObjectList)) COMMENT
            #272=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1629 
            100) COMMENT (CObjectList COMMENT #273=(CScratch COMMENT (CWidget 1 
            (1 #3# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 
            4.3983 1.9 COMMENT 0.082322 1) COMMENT #274=(CLabel COMMENT (CWidget
            0 (0 0) 1 2 0 0 #208# 30793204 100) COMMENT (LOGPEN 0 0 153) 1 
            COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"")
            1.74233 0.555556 128 COMMENT ""{\\i Bgl}I (1247)"" ""@N (@S)"" 5 
            13.8113 COMMENT -3.48466 0 -10 4.22286 0.958563 #273#)) COMMENT 
            (CObjectList)) COMMENT #275=(CGroupWidget COMMENT (CWidget 1 (10 #4#
            0) 1 2 0 0 Nil -1691 100) COMMENT (CObjectList COMMENT 
            #276=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #206# 0 100) 
            COMMENT (LOGPEN 0 12 10053171) 8 1 0.00300944 1.9 COMMENT 0.082322 
            1) COMMENT #277=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #208# 
            30306376 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 74 27 0 0 
            400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 
            COMMENT ""{\\i Bgl}II (4168)"" ""@N (@S)"" 5 -3.50465 COMMENT 
            17.4233 0 -10 4.62442 0.958563 #276#)) COMMENT (CObjectList)) 
            COMMENT #278=(CGroupWidget COMMENT (CWidget 1 (10 #5# 0) 1 2 0 0 Nil
            -1574 100) COMMENT (CObjectList COMMENT #279=(CScratch COMMENT 
            (CWidget 1 (1 #5# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 
            10053171) 8 1 6.26566 1.9 COMMENT 0.082322 1) COMMENT #280=(CLabel 
            (CWidget 0 (0 0) 1 2 0 0 #208# 1 100) COMMENT (LOGPEN 0 0 153) 1 
            COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"")
            1.74233 0.555556 128 COMMENT ""{\\i Cla}I (6)"" ""@N (@S)"" 5 
            2.83302 19.1656 COMMENT 0 -10 2.97932 0.958563 #279#)) 
            (CObjectList)) COMMENT #281=(CGroupWidget COMMENT (CWidget 1 (10 #6#
            0) 1 2 0 0 Nil -548 100) COMMENT (CObjectList COMMENT #282=(CScratch
            COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 
            12 10053171) 8 1 0.0165519 1.9 COMMENT 0.082322 1) COMMENT 
            #283=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) COMMENT (LOGPEN 0
            0 153) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT 
            ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Eco}RI (4159)"" 
            ""@N (@S)"" 5 -3.76951 COMMENT 15.681 0 -10 5.00007 0.958563 #282#))
            COMMENT (CObjectList)) COMMENT #284=(CGroupWidget COMMENT (CWidget 1
            (10 #8# 0) 1 2 0 0 Nil -1225 100) COMMENT (CObjectList COMMENT 
            #285=(CScratch COMMENT (CWidget 1 (1 #8# 1) 1 2 0 0 #206# 0 100) 
            COMMENT (LOGPEN 0 12 10053171) 8 1 6.19494 1.9 COMMENT 0.082322 1) 
            COMMENT #286=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #208# 
            112321996 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 74 27 0 0
            400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 
            COMMENT ""{\\i Hin}dIII (53)"" ""@N (@S)"" 5 12.5229 COMMENT 6.96932
            0 -10 4.71509 0.958563 #285#)) COMMENT (CObjectList)) COMMENT 
            #287=(CGroupWidget COMMENT (CWidget 1 (10 #9# 0) 1 2 0 0 Nil 
            1550738791 100) COMMENT (CObjectList COMMENT #288=(CScratch COMMENT 
            (CWidget 1 (1 #9# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 
            10053171) 8 1 6.23406 1.9 COMMENT 0.082322 1) COMMENT #289=(CLabel 
            COMMENT (CWidget 0 (0 0) 1 2 0 0 #208# 30355616 100) COMMENT (LOGPEN
            0 0 153) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT 
            ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Kpn}I (27)"" ""@N 
            (@S)"" 5 3.51468 15.681 COMMENT 0 -10 3.71767 0.958563 #288#)) 
            (CObjectList)) COMMENT #290=(CGroupWidget COMMENT (CWidget 1 (10 
            #12# 0) 1 2 0 0 Nil -413 100) COMMENT (CObjectList COMMENT 
            #291=(CScratch COMMENT (CWidget 1 (1 #12# 1) 1 2 0 0 #206# 0 100) 
            COMMENT (LOGPEN 0 12 10053171) 8 1 2.61521 1.9 COMMENT 0.082322 1) 
            COMMENT #292=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #208# 30850848
            100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0
            0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i 
            Nde}I (2432)"" ""@N (@S)"" 5 -11.1149 COMMENT -8.71165 0 -10 4.58556
            0.958563 #291#)) COMMENT (CObjectList)) COMMENT #293=(CGroupWidget 
            COMMENT (CWidget 1 (10 #15# 0) 1 2 0 0 Nil -2296 100) COMMENT 
            (CObjectList COMMENT #294=(CScratch COMMENT (CWidget 1 (1 #15# 1) 1 
            2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 6.20698 1.9 
            COMMENT 0.082322 1) COMMENT #295=(CLabel (CWidget 0 (0 0) 1 2 0 0 
            #208# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 74 27 0 0 
            400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 
            COMMENT ""{\\i Pst}I (45)"" ""@N (@S)"" 5 8.20712 10.454 COMMENT 0 
            -10 3.35497 0.958563 #294#)) (CObjectList)) COMMENT 
            #296=(CGroupWidget COMMENT (CWidget 1 (10 #18# 0) 1 2 0 0 Nil -144 
            100) COMMENT (CObjectList COMMENT #297=(CScratch COMMENT (CWidget 1 
            (1 #18# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 
            6.22202 1.9 COMMENT 0.082322 1) COMMENT #298=(CLabel COMMENT 
            (CWidget 0 (0 0) 1 2 0 0 #208# 268786344 100) COMMENT (LOGPEN 0 0 
            153) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT 
            ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Sal}I (35)"" ""@N 
            (@S)"" 5 3.23155 12.1963 COMMENT 0 -10 3.35497 0.958563 #297#)) 
            (CObjectList)) COMMENT #299=(CGroupWidget COMMENT (CWidget 1 (10 
            #19# 0) 1 2 0 0 Nil -1086 100) COMMENT (CObjectList COMMENT 
            #300=(CScratch COMMENT (CWidget 1 (1 #19# 1) 1 2 0 0 #206# 0 100) 
            COMMENT (LOGPEN 0 12 10053171) 8 1 2.7085 1.9 COMMENT 0.082322 1) 
            COMMENT #301=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) COMMENT 
            (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 
            COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Sap}I 
            (2370)"" ""@N (@S)"" 5 -8.88663 COMMENT -10.454 0 -10 4.49488 
            0.958563 #300#)) COMMENT (CObjectList)) COMMENT #302=(CGroupWidget 
            COMMENT (CWidget 1 (10 #22# 0) 1 2 0 0 Nil -817 100) COMMENT 
            (CObjectList COMMENT #303=(CScratch COMMENT (CWidget 1 (1 #22# 1) 1 
            2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 6.23105 1.9 
            COMMENT 0.082322 1) COMMENT #304=(CLabel COMMENT (CWidget 0 (0 0) 1 
            2 0 0 #208# 30873176 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT 
            (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233
            0.555556 128 COMMENT ""{\\i Xba}I (29)"" ""@N (@S)"" 5 3.42226 
            13.9386 COMMENT 0 -10 3.71767 0.958563 #303#)) (CObjectList)) 
            COMMENT #305=(CGroupWidget COMMENT (CWidget 2 (10 #1# 0) 1 2 0 0 Nil
            -1360 100) COMMENT (CObjectList COMMENT #306=(CScratch COMMENT 
            (CWidget 1 (1 #1# 1) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 
            10053171) 8 1 2.88455 1.9 COMMENT 0.082322 1) COMMENT #307=(CLabel 
            (CWidget 0 (0 0) 1 2 0 0 #198# 0 100) COMMENT (LOGPEN 0 0 13408563) 
            1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT 
            ""Georgia"") 1.74233 0.555556 0 COMMENT ""{\\i Afl}III (2253)"" ""@N
            (@S)"" 5 -6.37164 COMMENT -12.1963 0 -10 4.81872 0.958563 #306#) 
            COMMENT #308=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #206# 0 
            100) COMMENT (LOGPEN 0 12 10053171) 8 1 1.55438 1.9 COMMENT 0.082322
            1) COMMENT #309=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #198# 
            30793848 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 74 27
            0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 0 
            COMMENT ""{\\i Afl}III (3137)"" ""@N (@S)"" 5 -14.5282 0 COMMENT 0 
            -10 4.67623 0.958563 #308#)) (CObjectList)) COMMENT 
            #310=(CGroupWidget COMMENT (CWidget 2 (10 #16# 0) 1 2 0 0 Nil -2162 
            100) COMMENT (CObjectList COMMENT #311=(CScratch COMMENT (CWidget 1 
            (1 #16# 1) 1 2 0 0 #206# 30416704 COMMENT 100) (LOGPEN 0 12 
            10053171) 8 1 0.651545 1.9 COMMENT 0.082322 1) COMMENT #312=(CLabel 
            COMMENT (CWidget 0 (0 0) 1 2 0 0 #198# 30873944 100) COMMENT (LOGPEN
            0 0 13408563) 1 COMMENT (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 
            COMMENT ""Georgia"") 1.74233 0.555556 0 COMMENT ""{\\i Pvu}II 
            (3737)"" ""@N (@S)"" 5 -13.6052 COMMENT 5.22699 0 -10 4.76691 
            0.958563 #311#) COMMENT #313=(CScratch COMMENT (CWidget 2 (1 #16# 2)
            1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 0.511605 1.9
            COMMENT 0.082322 1) COMMENT #314=(CLabel COMMENT (CWidget 0 (0 0) 1 
            2 0 0 #198# 112321208 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT 
            (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233
            0.555556 0 COMMENT ""{\\i Pvu}II (3830)"" ""@N (@S)"" 5 -12.6797 
            COMMENT 6.96932 0 -10 4.94826 0.958563 #313#)) COMMENT 
            (CObjectList)) COMMENT #315=(CGroupWidget COMMENT (CWidget 2 (10 
            #21# 0) 1 2 0 0 Nil -413 100) COMMENT (CObjectList COMMENT 
            #316=(CScratch COMMENT (CWidget 1 (1 #21# 1) 1 2 0 0 #206# 0 100) 
            COMMENT (LOGPEN 0 12 10053171) 8 1 6.19795 1.9 COMMENT 0.082322 1) 
            COMMENT #317=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #198# 30850652
            100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 74 27 0 0 400 
            0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 0 COMMENT 
            ""{\\i Sph}I (51)"" ""@N (@S)"" 5 10.5312 8.71165 COMMENT 0 -10 
            3.49746 0.958563 #316#) COMMENT #318=(CScratch COMMENT (CWidget 2 (1
            #21# 2) 1 2 0 0 #206# 0 100) COMMENT (LOGPEN 0 12 10053171) 8 1 
            2.21495 1.9 COMMENT 0.082322 1) COMMENT #319=(CLabel (CWidget 0 (0 
            0) 1 2 0 0 #198# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT 
            (LOGFONT 74 27 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233
            0.555556 0 COMMENT ""{\\i Sph}I (2698)"" ""@N (@S)"" 5 -12.4271 
            COMMENT -6.96932 0 -10 4.5467 0.958563 #318#)) COMMENT 
            (CObjectList))) (CObjectList)) COMMENT #320=(CGroupWidget (CWidget 
            14 (16 0) 1 2 0 0 Nil -682 100) COMMENT (CObjectList) (CObjectList))
            COMMENT #321=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -2431 100) 
            COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT 
            (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 
            31929056 COMMENT (CStringList) 0 12955056 (CObList)))
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    176..212
                     /note="P102 promoter"
     misc_feature    176..181
                     /note="-35"
     misc_feature    199..204
                     /note="-10"
     protein_bind    213..228
                     /bound_moiety="TALE sucA"
                     /note="TALEsucA Binding Site"
                     /note="lac O1 operator"
     RBS             261..266
                     /note="RBS"
     gene            275..985
                     /note="mCherry"
     misc_feature    1024..1448
                     /note="5S RNA, T1, T2, rrnB"
     CDS             1541..2398
                     /note="AmpR"
     misc_feature    2556..3175
                     /note="colE1"
ORIGIN
        1 cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc
       61 tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat
      121 aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac
      181 agctagctca gtcctaggta ctgtgctagc aatgacacta agacagtttc gaattcaaaa
      241 gatctatcga tcgaggatcc aggaggtaca atcaatggtg agcaagggcg aggaggataa
      301 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      361 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      421 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      481 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      541 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      601 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      661 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      721 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      781 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      841 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      901 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      961 cggcatggac gagctgtaca agtaaggtac ctctagagtc gacctgcagg catgcaagct
     1021 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     1081 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     1141 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     1201 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1261 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     1321 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa
     1381 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     1441 aaactctttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     1801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctacagcaa tggcaacaac
     2101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2701 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3181 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3241 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     3361 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     3421 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     3481 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3541 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3601 ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat
     3661 gcatttacgt tgcgaattga tctggtttga cagcttatca t
//
