LOCUS       Exported               11507 bp ds-DNA     circular SYN 11-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAI4_RK2rep
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Morten Norholm Group
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2017 from SnapGene 4.0.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      476..523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             657..1280
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(1442..2257)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(2258..2349)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2341..2350
                     /label=USER
     rep_origin      complement(2367..2368)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     misc_feature    2378..6936
                     /label=For cloning
     CDS             5649..6900
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="*MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG
                     SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKR
                     KTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTT
                     RNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFT
                     FYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKK
                     TSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRL
                     MCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDLVHCKR*GLVGSVPAGGSAQLSL
                     IHISSNVAPEVSPIRYKL"
     misc_feature    6951..6958
                     /label=USER2
     misc_RNA        complement(7179..7257)
                     /label=tracr RNA
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     repeat_region   7429..7464
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   7495..7530
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   7561..7596
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   7627..7662
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     protein_bind    7760..7778
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7785..7803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             7828..427
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg
       61 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga
      121 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt
      181 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga
      241 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt
      301 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct
      361 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg
      421 tgactgagat cccatgttac cggtatccta ggctgctgcc accgctgagc aataactagc
      481 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt
      541 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtattga cgccaacggg
      601 tcacacgggt acagtgctag cagaaacata tccatgaaat cccgccccga attcatatgt
      661 ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      721 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      781 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      841 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
      901 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
      961 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
     1021 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
     1081 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
     1141 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
     1201 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
     1261 aatgtgaaag tgggtcttaa gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     1321 ccgacgacaa gctgacgacc gggtctccgc aagtggcact tttcggggaa atgtgcgcgg
     1381 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt
     1441 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     1501 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     1561 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     1621 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     1681 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     1741 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     1801 gcgcctgagc gagacgaaat acgcggtcgc tgttaaaagg acaattacaa acaggaatcg
     1861 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     1921 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     1981 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     2041 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     2101 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     2161 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     2221 gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt
     2281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2341 ataaacaaat aggcatgcta gcgcagaata aatatctaac accgtgcgtg ttgactattt
     2401 tacctctggc ggtgataatg gttgcatgta ctaaggaggt tgtatggaac aacgagatgt
     2461 gtataagaga cagctgaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt
     2521 tgtggatacc tcgcggaaaa cttggccctc actgacagat gaggggcgga cgttgacact
     2581 tgaggggccg actcacccgg cgcggcgttg acagatgagg ggcaggctcg atttcggccg
     2641 gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc
     2701 ccacagatga tgtggacaag cctggggata agtgccctgc ggtattgaca cttgaggggc
     2761 gcgactactg acagatgagg ggcgcgatcc ttgacacttg aggggcagag tgctgacaga
     2821 tgaggggcgc acctattgac atttgagggg ctgtccacag gcagaaaatc cagcatttgc
     2881 aagggtttcc gcccgttttt cggccaccgc taacctgtct tttaacctgc ttttaaacca
     2941 atatttataa accttgtttt taatggttgc atgtactaag gaggttgtat ggaacaacga
     3001 gatgtgtata agagacagct gaaacgccgt cgaagccgtg tgctgagaga tcccctcata
     3061 atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt gcagccacga
     3121 gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg cctaagcgaa
     3181 ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg tcccaacctt
     3241 gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg gcgattagca
     3301 gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct agtccgtagg
     3361 caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa tagatattaa
     3421 caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaacc catggctcct
     3481 ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt gtcgagcgct
     3541 gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag attggattgc
     3601 ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata tcatgtaagt
     3661 tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt cctcttactc
     3721 gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact ttaaagccag
     3781 catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg tcggctagaa
     3841 attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc tcataaagag
     3901 taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagtcgt ttttgctcac
     3961 gaatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag ataaagctag
     4021 catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct aacaatcggt
     4081 cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg ccaagttcgg
     4141 taagagtgag agttttacag tcaagtaatg cgtggcaagc caacgttaag ctgttgagtc
     4201 gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata tcgagttcgc
     4261 acatcttgtt gtctgattat tgatttttcg cgaaaccatt tgatcatatg acaagatgtg
     4321 tatccacctt aacttaatga tttttaccaa aatcattagg ggattcatca gagccgtgtg
     4381 cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     4441 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     4501 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
     4561 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     4621 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     4681 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     4741 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     4801 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     4861 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     4921 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
     4981 ccaaaaatgg cagcgctggc agtccttgcc attgccggga tcggggcagt aacgggatgg
     5041 gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc cgcaggtgct ggcatcgaca
     5101 ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg gtggcggcct gcccttcact
     5161 tcggccgtcg gggcattcac ggacttcatg gcggggccgg caatttttac cttgggcatt
     5221 cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg gtgaattaat tccccggatc
     5281 cgtcagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
     5341 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     5401 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     5461 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     5521 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     5581 ggatctgatg gcgcagggga tcaagatcga cggatccggg gaattccggg gcaatcccgc
     5641 aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg
     5701 cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc
     5761 gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg
     5821 acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc
     5881 gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa
     5941 ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg
     6001 ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct
     6061 tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct
     6121 ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca
     6181 ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg
     6241 atgacgaact ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc
     6301 cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt
     6361 attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt
     6421 ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc
     6481 gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg
     6541 ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc
     6601 gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct
     6661 tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg
     6721 aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgacctgg
     6781 tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca gcacagctgt
     6841 ctcttataca catctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     6901 aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     6961 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     7021 gcgcacaccg actagcgaaa aaaccccgcc gaagcggggt tttttgcgaa aaaaagcacc
     7081 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatgctg
     7141 ttttgaatgg ttccgctagc actgtaccta ggactgagct agccgtcaaa agcagcgtat
     7201 ataccatggc cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc
     7261 caatggccca ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg
     7321 gtcgtgtttt agagctatgc tgttttgaat ggtcccaaaa catgaactag cgattagtcg
     7381 ctatgactta agttttagag ctatgctgtt ttgaatggtc ccaaaacaac cacactagag
     7441 aacatactgg ctaaatagtt ttagagctat gctgttttga atggtcccaa aacggttgga
     7501 ctcaagacga tagttaccgg atagttttag agctatgctg ttttgaatgg tcccaaaacg
     7561 gcgaaacccg acaggactat aaagataccg ttttagagct atgctgtttt gaatggtccc
     7621 aaaacaaaaa aaaaccccgc ccctgacagg gcggggtttt ttttttaatt aaagtctggt
     7681 cctcgagtct ggttataatc cctatcagtg atagagattg acatccctat cagtgataga
     7741 gatactgagc acagaaggag atatacatgg ataagaaata ctgagcacta tcagtgatag
     7801 agatactgag cacagaagga gatatacatg gataagaaat actcaatagg cttagatatc
     7861 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
     7921 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
     7981 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
     8041 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
     8101 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
     8161 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
     8221 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
     8281 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
     8341 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
     8401 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
     8461 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
     8521 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
     8581 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
     8641 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
     8701 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
     8761 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     8821 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     8881 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
     8941 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
     9001 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc
     9061 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
     9121 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat
     9181 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
     9241 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
     9301 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
     9361 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
     9421 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
     9481 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
     9541 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
     9601 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
     9661 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
     9721 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
     9781 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
     9841 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
     9901 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
     9961 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
    10021 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
    10081 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
    10141 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
    10201 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
    10261 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
    10321 cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa agacgattca
    10381 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
    10441 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
    10501 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
    10561 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
    10621 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
    10681 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
    10741 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
    10801 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
    10861 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
    10921 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
    10981 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
    11041 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
    11101 gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta
    11161 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
    11221 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
    11281 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga
    11341 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
    11401 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
    11461 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggc
//
