LOCUS       AAVS1_Puro_Tet3G_FS_v2    6663 bp      DNA     circular   2016-04-19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : AAVS1_Puro_FS_v2 [5441  nt] : (Acc65I[2554] / 
            NsiI[2032]) to pTRE3G-BI-Tet3G_v2_PCR [1214  nt] : (NsiI[285
            8] / Acc65I[452])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    complement(5606..6463)
                     /label=AP(R)
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= bla gene- Ap(r) determinant  \ 
     misc_feature    complement(149..307)
                     /label=ALPHA
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= lacZ' gene encoding alpa lacZ peptide  \ 
     misc_feature    4844..4844
                     /label=ORI
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= RNaseH cleavage point  \ 
     misc_feature    complement(5606..6463)
                     /label=AmpR
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4844..5431
                     /label=Ori
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(145..307)
                     /label=lacZa
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6464..6528
                     /label=bla promoter
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    320..1120
                     /label=HA-L
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1130..1155
                     /label=SA
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1236..1832
                     /label=PuroR (No ATG)
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3741..3746
                     /label=Unique SalI
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3753..3759
                     /label=HindIII RFLP
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3762..4601
                     /label=HA-R
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(1109..1120)
                     /label=15556
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3762..3773
                     /label=15590
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1840..2032
                     /label=SV40 LPA
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3316..3381
                     /label=3X FLAG
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3516..3740
                     /label=bGH Poly A
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3367..3381
                     /label=Enterokinase
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3391..3480
                     /label=Twin_Strep_Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3397..3420
                     /label=Strep Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3457..3480
                     /label=Strep Tag
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3421..3444
                     /label=(GGGS)2
                     /SerialCloner_Color=&h008040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3472..3477
                     /label=BstBI (for N-term tagging) (use pESG-IBA167 for linker)
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1833..1838
                     /label=XbaI (Blocked by Dam)
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3277..3296
                     /label=TEV (for C-term Tag)
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    309..313
                     /label=Blunted EcoRI
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4603..4607
                     /label=Blunted EcoRI
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4608..4610
                     /label=PvuII Half
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    306..308
                     /label=PvuII Half
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(3515..3535)
                     /label=pcDNA R3
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    881..903
                     /label=AAVS1 Cel1 F
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4030..4051
                     /label=AAVS1 CEL1 R
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(4661..4680)
                     /label=pUC19 R
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    218..237
                     /label=pUC19 F
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1162..1181
                     /label=2A SEQ F (1 SNP in primer BS)
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1953..1972
                     /label=SV40 LPA SEQ F
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1170..1232
                     /label=2A
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3262..3267
                     /label=AflII
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3268..3275
                     /label=NotI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3299..3304
                     /label=NcoI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3304..3309
                     /label=BamHI (not unique)
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3310..3315
                     /label=NgoMIV
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3309..3316
                     /label=SgrAI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3500..3507
                     /label=PmeI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3508..3515
                     /label=SbfI
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2799..3249
                     /label=TRE3G-BI promoter
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="TRE3G-BI promoter" - 1..451 \ 
     misc_feature    complement(5603..6463)
                     /label=Amp(R)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="Amp(R)" - complement(1647..2507) \ 
     misc_feature    complement(6464..6568)
                     /label=pAmp(R)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pAmp(R)" - complement(2508..2612) \ 
     misc_feature    2038..2784
                     /label=Tet3G
                     /SerialCloner_Color=&h7F007F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2879..2897
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2915..2933
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2951..2969
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2987..3005
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3023..3041
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3059..3077
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3095..3113
                     /label=TetO
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagaa ttcgcccttt gctttctctg accagcattc tctcccctgg gcctgtgccg 
      361 ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc 
      421 cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc 
      481 tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc 
      541 tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc 
      601 catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc 
      661 tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt 
      721 gtaggcctgc atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt 
      781 tagccacctc tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg 
      841 ggtcacctct cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg 
      901 tgctagctct tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc 
      961 ttcttcctcc aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg 
     1021 gatcctgtgt ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg 
     1081 gttctgggta cttttatctg tcccctccac cccacagtgg ggcaagcttc tgacctcttc 
     1141 tcttcctccc acagggcctc gagagatctg gcggcggaga gggcagagga agtcttctaa 
     1201 catgcggtga cgtggaggag aatcccggcc ctagcaccga gtacaagccc acggtgcgcc 
     1261 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 
     1321 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 
     1381 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
     1441 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
     1501 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
     1561 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
     1621 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
     1681 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
     1741 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 
     1801 cctggtgcat gacccgcaag cccggtgcct gatctagaca gacatgataa gatacattga 
     1861 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 
     1921 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa 
     1981 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aatgcatTTA 
     2041 CCCGGGGAGC ATGTCAAGGT CAAAATCGTC AAGAGCGTCA GCAGGCAGCA TATCAAGGTC 
     2101 AAAGTCGTCA AGGGCATCGG CTGGGAGCAT GTCTAAGTCA AAATCGTCAA GGGCGTCGGT 
     2161 CGGCCCGCCG CTTTCGCACT TTAGCTGTTT CTCCAGGCCA CATATGATTA GTTCCAGGCC 
     2221 GAAAAGGAAG GCAGGTTCGG CTCCCTGCCG GTCGAACAGC TCAATTGCTT GTTTCAGAAG 
     2281 TGGGGGCATA GAATCGGTGG TAGGTGTCTC TCTTTCCTCT TTTGCTACTT GATGCTCCTG 
     2341 TTCCTCCAAT ACGCAGCCCA GTGTAAAGTG GCCCACGGCG GACAGAGCGT ACAGTGCGTT 
     2401 CTCCAGGGAG AAGCCTTGCT GACACAGGAA CGCGAGCTGA TTTTCCAGGG TTTCGTACTG 
     2461 TTTCTCTGTT GGGCGGGTGC CGAGATGCAC TTTAGCCCCG TCGCGATGTG AGAGGAGAGC 
     2521 ACAGCGGTAT GACTTGGCGT TGTTCCGCAG AAAGTCTTGC CATGACTCGC CTTCCAGGGG 
     2581 GCAGGAGTGG GTATGATGCC TGTCCAGCAT CTCGATTGGC AGGGCATCGA GCAGGGCCCG 
     2641 CTTGTTCTTC ACGTGCCAGT ACAGGGTAGG CTGCTCAACT CCCAGCTTTT GAGCGAGTTT 
     2701 CCTTGTCGTC AGGCCTTCGA TACCGACTCC ATTGAGTAAT TCCAGAGCAG AGTTTATGAC 
     2761 TTTGCTCTTG TCCAGTCTAG ACATGGTgaa ttctccaggc gatctgacgg ttcactaaac 
     2821 gagctctgct tatataggcc tcCCACCGTA CACGCCacct cgacatactc gagtttactc 
     2881 cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg 
     2941 cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat 
     3001 agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc 
     3061 cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag agaACGTATA 
     3121 AGCTTTAGGC GTGTACGGtg ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga 
     3181 tcgcctggag caattCCACA ACACTTTTGT CTTATACCaa ctttccgtac cacttcctac 
     3241 cctcgtaaag gtaccctcag ccttaaggcg gccgccgaga atctgtattt tcagggagcc 
     3301 atgggatccg ccggcgacta caaggaccac gacggcgatt ataaggatca cgacatcgac 
     3361 tacaaagacg acgatgacaa gggcgccagc agcgcctggt cccaccctca gtttgagaag 
     3421 ggcggaggct ctggcggcgg aagcggagga tctgcttgga gccaccccca gttcgaaaag 
     3481 tgataactcg agtctagacg tttaaaccct gcaggctgtg ccttctagtt gccagccatc 
     3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 
     3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 
     3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 
     3721 ggatgcggtg ggctctatgg gtcgacagta ctaagcttta ctagggacag gattggtgac 
     3781 agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg tctaaccccc 
     3841 acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg agccatctct 
     3901 ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc ctgggaggga 
     3961 gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt tctcagtggc 
     4021 caccctgcgc taccctctcc cagaacctga gctgctctga cgcggctgtc tggtgcgttt 
     4081 cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag tttggaaaaa 
     4141 caaaatcaga ataagttggt cctgagttct aactttggct cttcaccttt ctagtcccca 
     4201 atttatattg ttcctccgtg cgtcagtttt acctgtgaga taaggccagt agccagcccc 
     4261 gtcctggcag ggctgtggtg aggagggggg tgtccgtgtg gaaaactccc tttgtgagaa 
     4321 tggtgcgtcc taggtgttca ccaggtcgtg gccgcctcta ctccctttct ctttctccat 
     4381 ccttctttcc ttaaagagtc cccagtgcta tctgggacat attcctccgc ccagagcagg 
     4441 gtcccgcttc cctaaggccc tgctctgggc ttctgggttt gagtccttgg caagcccagg 
     4501 agaggcgctc aggcttccct gtcccccttc ctcgtccacc atctcatgcc cctggctctc 
     4561 ctgccccttc cctacagggg ttcctggctc tgctctaagg gcgaattctg cattaatgaa 
     4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     4801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     4861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     4921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     4981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     5041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     5101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     5161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     5221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     5281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     5341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     5401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     5461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     5521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     5581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     5641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     5701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     5761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     5821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     5881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     5941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     6001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     6061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     6121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     6181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     6241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     6301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     6361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     6421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     6481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     6541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 
     6601 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 
     6661 gtc
//