LOCUS       pEN435___pCAGGS_       11022 bp ds-DNA     circular     03-APR-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT       
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(9185..9867)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(239..307)
                     /label=LacZ alpha
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(239..307)
                     /label=LAcZalpha (serialcloner)
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=#64b1ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6153..6158
                     /label=SpeI
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     6160..6193
                     /label=FRT
                     /ApEinfo_fwdcolor=orchid
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1342..1343
                     /label=SbfI
                     /ApEinfo_fwdcolor=#b64983
                     /ApEinfo_revcolor=#10ef37
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    396..403
                     /label=SwaI
                     /ApEinfo_fwdcolor=#707b8f
                     /ApEinfo_revcolor=#707b8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     7750..7783
                     /label=FRT(1)
                     /ApEinfo_label=FRT
                     /ApEinfo_fwdcolor=orchid
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1344..1346)
                     /label=PacI
                     /ApEinfo_fwdcolor=#ff8080
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8781..8788
                     /label=SwaI(1)
                     /ApEinfo_label=SwaI
                     /ApEinfo_fwdcolor=#707b8f
                     /ApEinfo_revcolor=#707b8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6834..7432
                     /label=puro (variant)
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7792..7799
                     /label=SwaI(2)
                     /ApEinfo_label=SwaI
                     /ApEinfo_fwdcolor=#707b8f
                     /ApEinfo_revcolor=#707b8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6368..6766
                     /label=PGK promoter
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6255..6594
                     /label=mousePGK promoter (serialcloner)
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff8080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    404..1333
                     /label=TIGRE locus centromeric homology arm
                     /ApEinfo_fwdcolor=#e6f574
                     /ApEinfo_revcolor=#eff9ac
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1347..1352)
                     /label=Homology to pEN18
                     /ApEinfo_fwdcolor=#13ecc0
                     /ApEinfo_revcolor=#cf1ce3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7468..7695
                     /label=bGH pA (serialcloner)
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ffbf80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1347..1352)
                     /label=AvrII
                     /ApEinfo_fwdcolor=#a0eb94
                     /ApEinfo_revcolor=#a0eb94
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6834..7433
                     /label=puro (variant)(1)
                     /ApEinfo_label=puro (variant)
                     /ApEinfo_fwdcolor=#ffa980
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6778..6833
                     /label=EM7 promoter
                     /ApEinfo_fwdcolor=#0962f7
                     /ApEinfo_revcolor=#93b4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(4479..4498)
                     /label=pCAGGS-Forward Primer
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8797..8817)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(10864..10892)
                     /label=Amp Promoter (from Serial Cloner)
                     /ApEinfo_fwdcolor=#fec501
                     /ApEinfo_revcolor=#fce39a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7800..8780
                     /label=TIGRE locus telomeric homology arm
                     /ApEinfo_fwdcolor=#faf372
                     /ApEinfo_revcolor=#fdfabd
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(6035..6053)
                     /label=ROSA2
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(4458..6137)
                     /label=CAGGS Promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(6138..6152)
                     /label=CAGGS Promoter(1)
                     /ApEinfo_label=CAGGS Promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(6153..6158)
                     /label=CAGGS Promoter(2)
                     /ApEinfo_label=CAGGS Promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6153..6158
                     /label=homology to pEN24
                     /ApEinfo_fwdcolor=#bae01f
                     /ApEinfo_revcolor=#bae01f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1334..1341
                     /label=SwaI(3)
                     /ApEinfo_label=SwaI
                     /ApEinfo_fwdcolor=#707b8f
                     /ApEinfo_revcolor=#707b8f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    complement(8823..8845)
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9965..10624)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2275..3285)
                     /label=mCherry-hCdt1(30-120) G1 marker
                     /ApEinfo_fwdcolor=#ff6262
                     /ApEinfo_revcolor=#ffc0cb
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_site      complement(1733..2256)
                     /label=Rabbit globin polyA
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1689..1709
                     /label=M13-rev(1)
                     /ApEinfo_label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3727..4425)
                     /label=mTagBFP
                     /ApEinfo_fwdcolor=#2818e7
                     /ApEinfo_revcolor=#958df3
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    1661..1683
                     /label=LacO(1)
                     /ApEinfo_label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2272..2274)
                     /label=Stop\codon
                     /ApEinfo_fwdcolor=#00ff40
                     /ApEinfo_revcolor=#a4ffa4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_site      complement(1727..2256)
                     /label=Rabbit Globin pA
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3292..3354)
                     /label=T2A
                     /ApEinfo_fwdcolor=#c896f5
                     /ApEinfo_revcolor=#efe0fc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_site      complement(1538..1538)
                     /label=SV40 early pA
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3361..3720)
                     /label=Truncated hGeminin
                     /ApEinfo_fwdcolor=#3168ce
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3361..3720)
                     /label=AmCyan1-hGeminin\(1-110) SG2M marker
                     /ApEinfo_fwdcolor=#0ecaf1
                     /ApEinfo_revcolor=#64ddf7
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtATTTA AATACTGCCC CTAGGAGATG
      421 GATactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaaTTA AAGTTACTGC
     1321 CATAACACCT AACATTTAAA TCCtaaccta ggcctccaaa aaagcctcct cactacttct
     1381 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     1441 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     1501 gcgggactat ggttgctgac taattgagat gcggatcCGC TGGCACGACA GGTTTCCCGA
     1561 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC
     1621 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA
     1681 ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTTGGGCTGC AGGTCGAGGG
     1741 ATCTTCATAA GAGAAGAGGG ACAGCTATGA CTGGGAGTAG TCAGGAGAGG AGGAAAAATC
     1801 TGGCTAGTAA AACATGTAAG GAAAATTTTA GGGATGTTAA AGAAAAAAAT AACACAAAAC
     1861 AAAATATAAA AAAAATCTAA CCTCAAGTCA AGGCTTTTCT ATGGAATAAG GAATGGACAG
     1921 CAGGGGGCTG TTTCATATAC TGATGACCTC TTTATAGCCA CCTTTGTTCA TGGCAGCCAG
     1981 CATATGGCAT ATGTTGCCAA ACTCTAAACC AAATACTCAT TCTGATGTTT TAAATGATTT
     2041 GCCCTCCCAT ATGTCCTTCC GAGTGAGAGA CACAAAAAAT TCCAACACAC TATTGCAATG
     2101 AAAATAAATT TCCTTTATTA GCCAGAAGTC AGATGCTCAA GGGGCTTCAT GATGTCCCCA
     2161 TAATTTTTGG CAGAGGGAAA AAGATCTCAG TGGTATTTGT GAGCCAGGGC ATTGGCCACA
     2221 CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGCC gcttacccgg gttagatggt
     2281 gtcctggtcc tgcgcggatg tcaggtgggg cggctgacct gctgccgggg tggatttctt
     2341 tatcttctgg cccggagaag ggcaggctgg gatgtctggg gcctcggggg tactggggct
     2401 ggaaacctcg tccaccgaca gccgcagtct cctgcgggcc ggtggcctgg cctggtcgcg
     2461 tccgggggcg gcgggcgggc gggcgcgctt gcggctgcca ctggtagcgg aggccggggc
     2521 gcggagtgcg ggcctggcgg ggctgggctc gagcggccgc cagtgtgatg gatatccctt
     2581 gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac
     2641 gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc
     2701 gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc
     2761 gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta
     2821 catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga
     2881 ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga
     2941 gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
     3001 gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc
     3061 cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc
     3121 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
     3181 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
     3241 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatATGCA TGGGGCCCGG
     3301 GTTTTCCTCG ACGTCACCAC ATGTAAGAAG ACTCCCCCGT CCCTCGCCGC TTCCCTTAAG
     3361 cagcgccttt ctccgttttt ctgccacttc cttccaatac tgagaggatg gattttcttt
     3421 aatcataaga tcaaatgact cctgggtgac tcctccaaga tttttatttt cactagattc
     3481 tgggacaata accccagggc tggaagttgt agatgttaag tggtcattcc gatgtttcct
     3541 tttggacaag cctgcggaca gctcattttc tcttccaaca agagatccag atgcagaagg
     3601 ctgaatcatc ttcagagttc ttcttgggac agaactattc tttatattct ctttgatttc
     3661 ttcttgtttc tgcttcatac tgggattcat ctcgagcggc cgccagtgtg atggatatcc
     3721 CCTAGGATTA AGCTTGTGCC CCAGTTTGCT AGGGAGGTCG CAGTATCTGG CCACTGCCAC
     3781 CTCGTGCTGC TCGACGTAGG TCTCGTTGTT GGCCTCCTTG ATTCTTTCCA GTCTGTAGTC
     3841 CACATAGTAG ACGCCAGGCA TCTTGAGGTT CTTAGCGGGT TTCTTGGATC TATATGTGGT
     3901 CTTGATGTTT GCGATCAGAT GGCTCCCGCC CACGAGCTTC AGGGCCATGT CGTTTCTGCC
     3961 TTCCAGGCCG CCGTCAGCGG GGTACAGCGT CTCGGTGAAG GCCTCCCAGC CGAGTGTTTT
     4021 CTTCTGCATC ACAGGGCCGT TGGATGTGAA GTTCACCCCT CTGATCTTGA CGTTGTAGAT
     4081 GAGGCAGCCG TCCTGGAGGC TGGTGTCCTG GGTAGCGGTC AGCACGCCCC CGTCTTCGTA
     4141 TGTGGTGACT CTCTCCCATG TGAAGCCCTC AGGGAAGGAC TGCTTGAAGA AGTCGGGGAT
     4201 GCCCTGGGTG TGGTTGATGA AGGTCTTGCT GCCGTAGAGG AAGCTAGTAG CCAGGATGTC
     4261 GAAGGCGAAG GGGAGAGGGC CGCCCTCGAC CACCTTGATT CTCATGGTCT GGGTGCCCTC
     4321 GTAGGGCTTG CCTTCGCCCT CGGATGTGCA CTTGAAGTGA TGGTTGTCCA CGGTGCCCTC
     4381 CATGTACAGC TTCATGTGCA TGTTCTCCTT AATCAGCTCG CTCATGGTGG CGacgcgtga
     4441 gaattctcga ggaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc
     4501 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
     4561 gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc
     4621 tgcggcagcc ccgaggctgg agatggagaa ggggacggcg gcgcggcgac gcacgaaggc
     4681 cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgcccgcgcc cgctagaggg
     4741 ggtgcggcgg cgcctcccag atttcggctc cgccagattt gggacaaagg aagtccctgc
     4801 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg
     4861 ccggcgctcc ggggccgccg cgcccctccc ccgagccctc cccggcccga ggcggccccg
     4921 ccccgcccgg cacccccacc tgccgccacc ccccgcccgg cacggcgagc cccgcgccac
     4981 gccccgcacg gagccccgca cccgaagccg ggccgtgctc agcaactcgg ggaggggggt
     5041 gcaggggggg gttacagccc gaccgccgcg cccacacccc ctgctcaccc ccccacgcac
     5101 acaccccgca cgcagccttt gttcccctcg cagccccccc gcaccgcggg gcaccgcccc
     5161 cggccgcgct cccctcgcgc acacgcggag cgcacaaagc cccgcgccgc gcccgcagcg
     5221 ctcacagccg ccgggcagcg cgggccgcac gcggcgctcc ccacgcacac acacacgcac
     5281 gcaccccccg agccgctccc ccccgcacaa agggccctcc cggagccctt taaggctttc
     5341 acgcagccac agaaaagaaa cgagccgtca ttaaaccaag cgctaattac agcccggagg
     5401 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg
     5461 gcggcgcgag gcggcgcgga gcggggcacg gggcgaaggc aacgcagcga ctcccgcccg
     5521 ccgcgcgctt cgctttttat agggccgccg ccgccgccgc ctcgccataa aaggaaactt
     5581 tcggagcgcg ccgctctgat tggctgccgc cgcacctctc cgcctcgccc cgccccgccc
     5641 ctcgccccgc cccgccccgc ctggcgcgcg cccccccccc ccccgccccc atcgctgcac
     5701 aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga
     5761 gagtgaagca gaacgtgggg ctcacctcga cccatggtaa tagcgatgac taatacgtag
     5821 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     5881 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     5941 gccaagtggg cagtttaccg taaatagtcc acccattgac gtcaatggaa agtccctatt
     6001 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     6061 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     6121 actaatgacc ccgtaattga ttactattaa taactagtcg aagttcctat tctctagaaa
     6181 gtataggaac ttcaggtctg aagaggagtt tacgtccagc caagctagct tggctgcagg
     6241 tcgtcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     6301 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     6361 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     6421 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     6481 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     6541 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     6601 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     6661 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     6721 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcctgtt gacaattaat
     6781 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatgaccg
     6841 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     6901 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     6961 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     7021 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     7081 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     7141 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     7201 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     7261 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     7321 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     7381 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaggggatc
     7441 aattctctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7501 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7561 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7621 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7681 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgact agagcttgcg
     7741 gaacccttcg aagttcctat tctctagaaa gtataggaac ttcGGCGCGC CATTTAAATT
     7801 TTGGGATGTT TTCTGTTTGT TTTgagatag ggtttttcag tgtagtcctg gctgtcctag
     7861 aactcactct gtagaccagg ctggcctgga actcaaggac tgccagcctc tgcctcctga
     7921 gcatcgggat taaaggtgtg tgccaccact gcccagcaga gtgcgttttt actcttcact
     7981 ttgtgggtcc tggctgcctg agattgtgtg cttgcaccat tagatgggag ggatgacttg
     8041 ggaaagggtt gagtcttttg tgagtcttgc ggaggcccag gagggggtac gtaaattaga
     8101 gttctaagag gcccagcaaa agtgaacaag tgactggcca ctgtgagttc actgggggac
     8161 ctgagagtta actagtgaaa aggagaggtc ctagtggaga agctaaagaa ttaaagttac
     8221 tgccttgaag ttctttgtga aggccaagct ctggagcgcg gcttgaaaag cctttatccc
     8281 agagcagcat gggaaggggc cccagctggg tgcgagcctg caaaccagag cacacctatc
     8341 acagggcctt ttcttaccag tgggacggga tcaaggcgga gcttacctgg gaacgtacag
     8401 ggcggaacct taacttgggg gcgtggccga atcacctggg tttggaaacg gttcttgtct
     8461 ttcctcaagc tcaggagagg ttgtttgcag gacactggcc cggaggggcc cgaactctcc
     8521 gagggagggt ttcgtggatc agaacaccgt cccttgacaa aaagatgcag agcaccgtct
     8581 gcattctgct agtaaccgtc cctcctgggg gcggagccag ccctcctccg ggtgtataaa
     8641 gtagctgagc gcctccttct gacaaacgcc tcacccccaa gagtactggg ggagtacgca
     8701 gcacgccagc ctaggggcgg agctcagccc tggaggaggc gtttcttctt agttctccga
     8761 CTCACGCCTC TCCACTTTTC ATTTAAATgg cgtaatcatg gtcatagctg tttcctgtgt
     8821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9001 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10921 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10981 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc 
//
