LOCUS       Exported                6145 bp ds-DNA     circular SYN 02-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    iRFP-PAmCh-PH_PLCd1 for addgene
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Chen Ji
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 2, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
     promoter        365..568
                     /label=CMV promoter
     misc_feature    591
                     /label=MCS
     CDS             610..1557
                     /codon_start=1
                     /label=iRFP
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             1573..2280
                     /codon_start=1
                     /label=PAmCherry1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2293..2808
                     /codon_start=1
                     /label=PH_PLC-delta-1(human)
                     /translation="GMDSGRDFLTLHGLQDDEDLQALLKGSQLLKVKSSSWRRERFYKL
                     QEDCKTIWQESRKVMRTPESQLFSIEDIQEVRMGHRTEGLEKFARDVPEDRCFSIVFKD
                     QRNTLDLIAPSPADAQHWVLGLHKIIHHSGSMDQRQKLQHWIHSCLRKADKNKDNKMSF
                     KELQNFLKE"
     polyA_signal    2933..3054
                     /label=SV40 poly(A) signal
     rep_origin      complement(3061..3516)
                     /direction=LEFT
                     /label=f1 ori
     promoter        3543..3647
                     /label=AmpR promoter
     promoter        3649..4006
                     /label=SV40 promoter
     rep_origin      3857..3992
                     /label=SV40 ori
     CDS             4041..4835
                     /codon_start=1
                     /label=NeoR/KanR
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5067..5114
                     /label=HSV TK poly(A) signal
     rep_origin      5443..6031
                     /direction=RIGHT
                     /label=ori
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggta
      601 cccgccacca tggctgaagg atccgtcgcc aggcagcctg acctcttgac ctgcgacgat
      661 gagccgatcc atatccccgg tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc
      721 gacatgacga tcgttgccgg cagcgacaac cttcccgaac tcaccggact ggcgatcggc
      781 gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg
      841 atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg
      901 cgaaaggacg caggcttcat cggctcctgg catcgccatg atcagctcat cttcctcgag
      961 ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc
     1021 gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa
     1081 gaggtgcgga agattaccgg cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc
     1141 agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg agtcaaaact aggcctgcac
     1201 tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg
     1261 atcattcccg atatcaatta tcggccggtg ccggtcaccc cagacctcaa tccggtcacc
     1321 gggcggccga ttgatcttag cttcgccatc ctgcgcagcg tctcgcccgt ccatctggaa
     1381 ttcatgcgca acataggcat gcacggcacg atgtcgatct cgattttgcg cggcgagcga
     1441 ctgtggggat tgatcgtttg ccatcaccga acgccgtact acgtcgatct cgatggccgc
     1501 caagcctgcg agctagtcgc ccaggttctg gcctggcaga tcggcgtgat ggaagagcca
     1561 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat taaggagttc
     1621 atgcgcttca aggtgcacat ggaggggtcc gtgaacggcc acgtgttcga gatcgagggc
     1681 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     1741 ggccccctgc ccttcacctg ggacatcctg agccctcagt tcatgtacgg ctccaatgcc
     1801 tacgtgaagc accccgccga catccccgac tactttaagc tgtccttccc cgagggcttc
     1861 aagtgggagc gcgtgatgaa attcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     1921 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1981 gacggccccg tgatgcagaa gaagaccatg ggctgggagg ccctctccga gcggatgtac
     2041 cccgaggacg gcgccctgaa gggcgaggtc aagcccagag tgaagctgaa ggacggcggc
     2101 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2161 gcctacaacg tcaaccgcaa gctggacatc accagccaca acgaggacta caccatcgtg
     2221 gagcagtacg agagagccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     2281 agatctcccg ggggcatgga ctcgggccgg gacttcctga ccctgcacgg cctacaggat
     2341 gatgaggatc tacaggcgct gctgaagggc agccagctcc tgaaggtgaa gtccagctca
     2401 tggaggagag agcgcttcta caagttgcag gaggactgca agaccatctg gcaggagtcc
     2461 cgcaaggtca tgcggacccc ggagtcccag ctgttctcca tcgaggacat tcaggaggtg
     2521 cgaatggggc accgcacgga gggtctggag aagttcgccc gtgatgtgcc cgaggaccgc
     2581 tgcttctcca ttgtcttcaa ggaccagcgc aatacactag acctcatcgc cccatcgcca
     2641 gctgatgccc agcactgggt gctggggctg cacaagatca tccaccactc aggctccatg
     2701 gaccagcgtc agaagctaca gcactggatt cactcctgct tgcgaaaagc tgacaaaaac
     2761 aaggacaaca agatgagctt caaggagctg cagaacttcc tgaaggagta agcggccgcg
     2821 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2881 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     3061 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3121 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3181 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3241 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3301 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3361 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3421 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3481 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3541 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3601 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     3661 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3781 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3841 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3901 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3961 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4021 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4081 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4141 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4201 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     4261 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4321 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4381 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4441 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4501 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4561 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4621 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4681 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4741 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4801 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4861 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4921 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4981 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5041 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     5101 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5161 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     5221 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     5281 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6121 ggataaccgt attaccgcca tgcat
//
