LOCUS Exported 6127 bp ds-DNA circular SYN 02-MAY-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS iRFP-PAmCherry1-PHgrp1 for Addgene SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 6127) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, May 2, 2017 from SnapGene Viewer 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6127 /organism="recombinant plasmid" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 610..1557 /codon_start=1 /product="phytochrome-based near-infrared fluorescent protein (Filonov et al., 2011)" /label=iRFP /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE" CDS 1573..2280 /codon_start=1 /product="photoactivatable monomeric derivative of DsRed fluorescent protein (Subach et al., 2009)" /label=PAmCherry1 /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" CDS 2293..2751 /codon_start=1 /label=PH-GRP1 /translation="PFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDN CLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRV VEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK" polyA_signal 2915..3036 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3043..3498) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3525..3629 /gene="bla" /label=AmpR promoter promoter 3631..3988 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3839..3974 /label=SV40 ori /note="SV40 origin of replication" CDS 4023..4817 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5049..5096 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5425..6013 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggta 601 cccgccacca tggctgaagg atccgtcgcc aggcagcctg acctcttgac ctgcgacgat 661 gagccgatcc atatccccgg tgccatccaa ccgcatggac tgctgctcgc cctcgccgcc 721 gacatgacga tcgttgccgg cagcgacaac cttcccgaac tcaccggact ggcgatcggc 781 gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg agacgcacaa ccgtctgacg 841 atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga tcactgtcgg cttcacgatg 901 cgaaaggacg caggcttcat cggctcctgg catcgccatg atcagctcat cttcctcgag 961 ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt tcttccgccg caccaacagc 1021 gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg cctgcgccgc cgcggcgcaa 1081 gaggtgcgga agattaccgg cttcgatcgg gtgatgatct atcgcttcgc ctccgacttc 1141 agcggcgaag tgatcgcaga ggatcggtgc gccgaggtcg agtcaaaact aggcctgcac 1201 tatcctgcct caaccgtgcc ggcgcaggcc cgtcggctct ataccatcaa cccggtacgg 1261 atcattcccg atatcaatta tcggccggtg ccggtcaccc cagacctcaa tccggtcacc 1321 gggcggccga ttgatcttag cttcgccatc ctgcgcagcg tctcgcccgt ccatctggaa 1381 ttcatgcgca acataggcat gcacggcacg atgtcgatct cgattttgcg cggcgagcga 1441 ctgtggggat tgatcgtttg ccatcaccga acgccgtact acgtcgatct cgatggccgc 1501 caagcctgcg agctagtcgc ccaggttctg gcctggcaga tcggcgtgat ggaagagcca 1561 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat taaggagttc 1621 atgcgcttca aggtgcacat ggaggggtcc gtgaacggcc acgtgttcga gatcgagggc 1681 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc 1741 ggccccctgc ccttcacctg ggacatcctg agccctcagt tcatgtacgg ctccaatgcc 1801 tacgtgaagc accccgccga catccccgac tactttaagc tgtccttccc cgagggcttc 1861 aagtgggagc gcgtgatgaa attcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 1921 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1981 gacggccccg tgatgcagaa gaagaccatg ggctgggagg ccctctccga gcggatgtac 2041 cccgaggacg gcgccctgaa gggcgaggtc aagcccagag tgaagctgaa ggacggcggc 2101 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 2161 gcctacaacg tcaaccgcaa gctggacatc accagccaca acgaggacta caccatcgtg 2221 gagcagtacg agagagccga gggccgccac tccaccggcg gcatggacga gctgtacaag 2281 agatctcgag gaccgtttaa gatcccagaa gacgacggaa atgacctgac acacacgttc 2341 ttcaacccag accgagaagg ctggctgctg aagctggggg gtcgtgtgaa gacctggaaa 2401 cggcgctggt tcatcctcac agataactgc ctctactact ttgagtacac cacggacaag 2461 gagcccaggg gcatcatccc cctggagaac ctcagcatca gggaggtgga ggacccccgg 2521 aagccgaact gctttgagct gtataacccc agtcacaaag gtcaagtcat caaggcctgc 2581 aagacggagg ccgatggccg tgtggtggag ggcaaccacg ttgtgtaccg gatctctgcc 2641 cccagcccgg aggaaaagga ggagtggatg aagtccatca aagcaagcat cagtagggac 2701 ccgttctatg acatgttggc cacgaggaaa aggaggattg ccaataagaa ataggaattc 2761 tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag 2821 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 2881 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 2941 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 3001 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata 3061 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 3121 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 3181 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 3241 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 3301 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 3361 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 3421 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 3481 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 3541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 3601 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 3961 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 4021 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 4081 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 4141 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 4201 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 4261 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 4321 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 4381 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 4441 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 4501 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 4561 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 4621 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 4681 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 4741 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 4801 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 4861 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 4921 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 4981 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 5041 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 5101 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 5161 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 5221 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 6061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 6121 catgcat //