LOCUS       Exported               10626 bp ds-DNA     circular SYN 09-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pINDUCER20-SNCA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 9, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pInducer\20 11791 bp DNA circular 4-MAR-2011 SOURCE ORGANISM 
            COMMENT This file is created by Vector NTI 
            http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT 
            VNTDATE|583771463| COMMENT VNTDBDATE|583771475| COMMENT LSOWNER| 
            COMMENT VNTNAME|pInducer 20| COMMENT VNTAUTHORNAME|Demo User| 
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FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="HIV-1 LTR"
     misc_feature    686..823
                     /label=psi
                     /note="packaging signal; psi"
     misc_feature    1310..1514
                     /label=RRE
                     /note="Rev responsive element; RRE"
     promoter        2217..2650
                     /label=TRE2
     misc_feature    2672..2691
                     /note="pINDUCER_for"
     misc_feature    2701..2725
                     /label=attB1
                     /note="recognized as attB1"
     misc_RNA        2740
                     /note="EcoRI"
     misc_feature    2741..3160
                     /note="SNCA"
     CDS             3161..3190
                     /codon_start=1
                     /label=Myc tag
                     /translation="EQKLISEEDL"
     misc_feature    3210..3234
                     /label=attB2
                     /note="recognized as attB2"
     misc_feature    3287..3313
                     /label=HA tag
     promoter        3399..4610
                     /label=Ubc
     misc_feature    complement(3527..3546)
                     /note="UBC promoter_up"
     misc_feature    4669..5415
                     /label=rtTA
     misc_feature    5451..6022
                     /label=IRES
     CDS             6043..6837
                     /label=Neo
     misc_feature    complement(6368..6385)
                     /label=Neo-R
                     /note="Genewiz"
     misc_feature    6707..6726
                     /label=Neo-F
                     /note="Genewiz"
     enhancer        7023..7598
                     /label=WPRE
                     /note="Regulatory element of woodchuck hepatitis virus"
     LTR             7714..7912
                     /label=3' LTR
                     /note="RU5"
     CDS             8240..9079
                     /label=Amp
     rep_origin      complement(9177..9859)
                     /direction=LEFT
                     /note="pBM1(ColE1) origin"
     misc_feature    10104..10123
                     /label=SV40-promoter
                     /note="Genewiz"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctaagct tgctcgaggg atggatgtat tcatgaaagg
     2761 actttcaaag gccaaggagg gagttgtggc tgctgctgag aaaaccaaac agggtgtggc
     2821 agaagcagca ggaaagacaa aagagggtgt tctctatgta ggctccaaaa ccaaggaggg
     2881 agtggtgcat ggtgtggcaa cagtggctga gaagaccaaa gagcaagtga caaatgttgg
     2941 aggagcagtg gtgacgggtg tgacagcagt agcccagaag acagtggagg gagcagggag
     3001 cattgcagca gccactggct ttgtcaaaaa ggaccagttg ggcaagaatg aagaaggagc
     3061 cccacaggaa ggaattctgg aagatatgcc tgtggatcct gacaatgagg cttatgaaat
     3121 gccttctgag gaagggtatc aagactacga acctgaagcc gagcagaagc tgatcagcga
     3181 ggaggacctg tgagcccggt gcggccgcaa cccagctttc ttgtacaaag tggttgatat
     3241 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaatacc catacgacgt
     3301 cccagactac gcttagtaat gattaattaa actagaaatt ctaccgggta ggggaggcgc
     3361 ttttcccaag gcagtctgga gtaacccacc caagatctgg cctccgcgcc gggttttggc
     3421 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     3481 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     3541 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     3601 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     3661 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     3721 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     3781 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     3841 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     3901 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     3961 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     4021 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     4081 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     4141 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     4201 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     4261 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     4321 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     4381 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     4441 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     4501 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     4561 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggt
     4621 accgagctcg gatctccacc ccgtaccggt cctgcagtcg aattcaccat gtctagactg
     4681 gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc
     4741 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     4801 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     4861 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     4921 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     4981 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     5041 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     5101 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     5161 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     5221 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     5281 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     5341 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     5401 atgctccccg ggtaactaag taaggatccg cggccgcact agaggaattc cgcccctctc
     5461 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgtgtttg
     5521 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5581 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5641 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5701 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5761 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5821 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtagtcaac aaggggctga
     5881 aggatgccca gaaggtaccc cattgtatgg gaatctgatc tggggcctcg gtgcacatgc
     5941 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6001 gttttccttt gaaaaacacg atgataagct taccggtcca ccatgattga acaagatgga
     6061 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6121 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6181 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     6241 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6301 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6361 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6421 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6481 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6541 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     6601 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6661 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6721 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6781 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgatgt
     6841 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     6901 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     6961 tgttgttgtt tagtccctcc caattcgata tcaagcttat cgatcgatag atcctaatca
     7021 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7081 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7141 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7201 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7261 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7321 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7381 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7441 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7501 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7561 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     7621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7681 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7921 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7981 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     8041 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     8101 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8221 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8281 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8341 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8401 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8461 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8521 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8581 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8641 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8701 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8761 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8821 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8881 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8941 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9001 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9061 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9121 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9181 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9901 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9961 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10021 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
    10081 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10141 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    10201 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10261 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10321 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    10381 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10441 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    10501 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    10561 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    10621 agtagt
//
