LOCUS       Exported                8572 bp ds-DNA     circular SYN 09-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBabe-CMV-MAPT-YFP(1-155)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 9, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     enhancer        1578..1957
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1958..2161
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2392..2410
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    2488..2512
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     2496..2512
                     /note="attL1"
     misc_feature    2534..3855
                     /note="Tau441"
     protein_bind    complement(3872..3896)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     3872..3881
                     /note="attL2"
     misc_feature    3961..4422
                     /label=YFP(1-155)
     promoter        4463..4962
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4976..5779
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     LTR             5853..6446
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(6818..7406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7584..8444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8445..8549)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatct cccgatcccc tatggtcgac
     1381 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     1441 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     1501 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     1561 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     1621 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1681 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1741 agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc
     1801 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1861 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1921 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1981 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2041 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2101 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taaccagagc
     2161 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2221 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
     2281 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
     2341 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     2401 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
     2461 ctgcacctcg gttctatcga ttgatcaaca agtttgtaca aaaaagcagg ctccgcggcc
     2521 gcccccttca ccatggctga gccccgccag gagttcgaag tgatggaaga tcacgctggg
     2581 acgtacgggt tgggggacag gaaagatcag gggggctaca ccatgcacca agaccaagag
     2641 ggtgacacgg acgctggcct gaaagaatct cccctgcaga cccccactga ggacggatct
     2701 gaggaaccgg gctctgaaac ctctgatgct aagagcactc caacagcgga agatgtgaca
     2761 gcacccttag tggatgaggg agctcccggc aagcaggctg ccgcgcagcc ccacacggag
     2821 atcccagaag gaaccacagc tgaagaagca ggcattggag acacccccag cctggaagac
     2881 gaagctgctg gtcacgtgac ccaagctcgc atggtcagta aaagcaaaga cgggactgga
     2941 agcgatgaca aaaaagccaa gggggctgat ggtaaaacga agatcgccac accgcgggga
     3001 gcagcccctc caggccagaa gggccaggcc aacgccacca ggattccagc aaaaaccccg
     3061 cccgctccaa agacaccacc cagctctggt gaacctccaa aatcagggga tcgcagcggc
     3121 tacagcagcc ccggctcccc aggcactccc ggcagccgct cccgcacccc gtcccttcca
     3181 accccaccca cccgggagcc caagaaggtg gcagtggtcc gtactccacc caagtcgccg
     3241 tcttccgcca agagccgcct gcagacagcc cccgtgccca tgccagacct gaagaatgtc
     3301 aagtccaaga tcggctccac tgagaacctg aagcaccagc cgggaggcgg gaaggtgcag
     3361 ataattaata agaagctgga tcttagcaac gtccagtcca agtgtggctc aaaggataat
     3421 atcaaacacg tcccgggagg cggcagtgtg caaatagtct acaaaccagt tgacctgagc
     3481 aaggtgacct ccaagtgtgg ctcattaggc aacatccatc ataaaccagg aggtggccag
     3541 gtggaagtaa aatctgagaa gcttgacttc aaggacagag tccagtcgaa gattgggtcc
     3601 ctggacaata tcacccacgt ccctggcgga ggaaataaaa agattgaaac ccacaagctg
     3661 accttccgcg agaacgccaa agccaagaca gaccacgggg cggagatcgt gtacaagtcg
     3721 ccagtggtgt ctggggacac gtctccacgg catctcagca atgtctcctc caccggcagc
     3781 atcgacatgg tagactcgcc ccagctcgcc acgctagctg acgaggtgtc tgcctccctg
     3841 gccaagcagg gtttggcccg gtgcggccgc aacccagctt tcttgtacaa agtggttgat
     3901 ccccggggat cctctagagt cgacggcgga ggaggttcag gaggcggagg ttcgctgcat
     3961 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4021 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4081 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4141 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     4201 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4261 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4321 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4381 ctggagtaca actacaacag ccacaacgtc tatatcatgg cctagaagct aattcgttaa
     4441 cctcgagaga tctaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     4501 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     4561 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4621 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4681 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     4741 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgttc cttcgctttc
     4801 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     4861 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     4921 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag ccaatatggg
     4981 atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5041 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5101 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5161 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5221 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5281 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5341 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5401 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5461 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5521 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5581 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5641 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5701 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5761 tcttgacgag ttcttctgag gggatccgtc gacctgcagc caagcttatc gataaaataa
     5821 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     5881 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
     5941 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     6001 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     6061 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     6121 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     6181 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     6241 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     6301 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     6361 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     6421 ctacccgtca gcgggggtct ttcatttccg acttgtggtc tcgctgcctt gggagggtct
     6481 cctctgagtg attgactacc cgtcagcggg ggtcttcaca tgcagcatgt atcaaaatta
     6541 atttggtttt ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc
     6601 caacgcgcgg ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact
     6661 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7501 tcacctagat ccttttaaat taaaaatgaa gtttgcggcc gcaaatcaat ctaaagtata
     7561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     8221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
