LOCUS       Wi_(H.influenzae_MolC_( 7244 bp ds-DNA     circular     18-MAY-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    5341..5343
                     /label="STOP codon"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      7057..495
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4075..4094
                     /label="Wi-550For"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3508..3522
                     /label="Enterokinase cleavage site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(4822..4842)
                     /label="Wi-240Rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4582..5340
                     /label="MolB (HI1470) ATPase protein"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_binding    3395..3417
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3132..3161
                     /label="pETN1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3469..3492
                     /label="8xHis Tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             6300..6959
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3526..4536
                     /label="MolC (HI1471) Transmembrane protein"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4537..4539
                     /label="STOP codon"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(6302..6329)
                     /label="pETC_R1"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     3376..3395
                     /label="T7 Forward"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(5417..5435)
                     /label="T7 Reverse"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
       61 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      541 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      601 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      661 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt
      721 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      781 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      841 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      901 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
      961 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     1021 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     1081 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     1141 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     1201 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     1261 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     1321 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     1381 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     1441 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     1501 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     1561 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     1621 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     1681 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     1741 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     1801 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     1861 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     1921 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1981 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     2041 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     2101 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     2161 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     2221 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     2281 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     2341 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     2401 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     2461 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     2521 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     2581 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     2641 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     2701 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     2761 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     2821 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     2881 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     2941 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     3001 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     3061 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     3121 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     3181 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     3241 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     3301 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     3361 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     3421 cTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CCATGGGCCA TCATCATCAT
     3481 CATCATCATC ACAGCAGCGG CCATATCGAC GACGACGACA AGCATatgca gccagatagt
     3541 taccctaaaa ttctctttgg cttaacgctg ttgcttgtga ttaccgccgt gatttctttg
     3601 ggaatagggc ggtattcttt gtctgtaccg caaattggtc aaattctgtg ggcaaaagcg
     3661 accgcacttg agatcgatcc agtgcagcaa caggtgattt ttcaagtgcg tttacctcga
     3721 attttaaccg cactttgcgt gggtgccggt ttggcattga gtggtgtggt attgcaaggc
     3781 atttttcgta acccgttagt gaatccgcat attatcggcg tgacctccgg ttcagccttc
     3841 ggtggcacct tggcaatttt ctttggattc agtttatacg gcttatttac cagcactatt
     3901 ttattcggct tcggcacgct tgccttggtt tttctattta gcttcaaatt taatcaacgc
     3961 agcttgttga tgcttattct tatcggcatg attttaagcg gacttttttc agccctagtg
     4021 agcctattgc aatacatttc cgatacagaa gaaaaattgc caagcattgt gttttggttg
     4081 atggggagtt ttgccacctc taactgggaa aaactcctct ttttctttgt cccgtttttg
     4141 ctgtgtagct cgattttgct cagcttaagt tggcgcttga atttactgtc tttagatgag
     4201 aaagaagcca aggctttggg cgtgaaaatg gccccattac gttggttagt gatcttttta
     4261 agcggttcgt tagtggcatg ccaggtggca attagcggca gtatcggctg ggtcgggctg
     4321 atcattcctc atcttagccg tatgctggta ggtgccaatc atcaaagttt attgccttgc
     4381 accatgcttg ttggggcgac ttatatgttg cttgtggaca atgttgcgcg ttccctatcc
     4441 gatgccgaaa ttccaatcag tattttgacc gcacttattg gtgcaccgtt gtttggtgta
     4501 ttggtatata aactaaaacg cggaggcatg aatgaataat ctagaaataa ttttgtttaa
     4561 ctttaagaag gagatataca tatgaataaa gcgctctccg tggaaaatct aggtttttat
     4621 taccaagcgg aaaacttcct gtttcagcaa ctgaattttg atttgaataa aggagatatt
     4681 ctggcggttt tagggcaaaa cggttgcggg aaaagtacct tgttggattt gttgcttggc
     4741 attcatcgcc cgattcaggg caagattgag gtgtatcaat ctatcggttt tgtgccgcaa
     4801 ttcttttctt cgccttttgc ttattccgta ttggatatcg tgttgatggg gcgctcaacc
     4861 catattaata cttttgccaa accgaaatcg catgattatc aagtcgccat gcaggcactg
     4921 gattatttga acttgaccca tttggcaaag cgcgaattta cttcgctttc gggcgggcag
     4981 cgacaactaa ttttaattgc gagagccatt gcttcagaat gcaaattgat actgttggat
     5041 gagccaactt cagcattaga tcttgcaaat caagatattg tactgtcttt acttatcgat
     5101 ttggcacaat cacagaacat gactgtcgta ttcacaacac atcaacctaa tcaagttgta
     5161 gcgatagcca ataaaacttt attgttaaat aaacaaaatt ttaaatttgg cgaaacaagg
     5221 aatattttaa cctcagaaaa tttgaccgca ctttttcatt taccgatgtt tgaacaacaa
     5281 gctcaatata aagaatcttt ttttacgcat tttgttccct tgtataaaac gttattaaag
     5341 TAAgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
     5401 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     5461 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
     5521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5581 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5701 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5761 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5821 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5881 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5941 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
     6001 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6061 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6121 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6181 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6241 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6301 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6361 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6421 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6481 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6541 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6601 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     6661 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6721 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6781 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6841 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6901 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6961 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7021 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggta
//