LOCUS       108B_V5_CaMO_NoH        5480 bp ds-DNA     circular     08-DEC-2015
SOURCE      
  ORGANISM  
COMMENT     NewMCS:XbaI,BamHI,NheI,AgeI,BstBI,AfeI(blunt),HpaI(blunt),AscI(8cut
            ter),SbfI,EcoRIOtherpossibleuniquecutters(currentlynosites)BsiWI,Bm
            tI(samesequenceasNheI)Tochangepromoter cutwith PacIand XbaI
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2744..2884
                     /label=ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..130
                     /label=ITR(1)
                     /ApEinfo_label=ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1188..1331,1338..1370)
                     /label=TEV for yeast
                     /ApEinfo_fwdcolor=#ff51d8
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    642..650
                     /label=Kozak consensus 
                     /ApEinfo_fwdcolor=#ffd92b
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1134..1163
                     /label=GGSGSGGSGSGSGGS linker
                     /ApEinfo_fwdcolor=#fff679
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(651..701,705..749,753..1091)
                     /label=CaM from GCaMP5
                     /ApEinfo_fwdcolor=#ff385c
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1862..2470
                     /label=WPRE
                     /ApEinfo_fwdcolor=#a0ff99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    702..704
                     /label=F19L
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    750..752
                     /label=V35G
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1848..1853
                     /label=EcoRI
                     /ApEinfo_fwdcolor=#46ff6e
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2484..2715
                     /label=PolyA
                     /ApEinfo_fwdcolor=#ff2549
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1092..1099,1101..1105,1107..1111,1113..1132)
                     /label=V5 tag
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    648..650
                     /label=start codon
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1188..1370
                     /label=S219V-tev in BL21 DNA
                     /ApEinfo_fwdcolor=#a486ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1371..1844
                     /label=TEV for yeast(1)
                     /ApEinfo_label=TEV for yeast
                     /ApEinfo_fwdcolor=#ff51d8
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    151..628
                     /label=synapsin promoter
                     /ApEinfo_fwdcolor=#4eff51
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1371..1844
                     /label=S219V-tev in BL21 DNA(1)
                     /ApEinfo_label=S219V-tev in BL21 DNA
                     /ApEinfo_fwdcolor=#a486ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaGGATC Cgccaccatg GACCAACTGA
      661 CTGAAGAGCA GATCGCAGAA TTTAAAGAGG CTTTCTCCCT ActgGACAAG GACGGGGATG
      721 GGACAATAAC AACCAAGGAG CTGGGGACGg ggATGCGGTC TCTGGGGCAG AACCCCACAG
      781 AAGCAGAGCT GCAGGACATG ATCAATGAAG TAGATGCCGA CGGTGACGGC ACAATCGACT
      841 TCCCTGAGTT CCTGACAATG ATGGCAAGAA AAATGAAATA CACAGACAGT GAAGAAGAAA
      901 TTAGAGAAGC GTTCCGTGTG TTTGATAAGG ATGGCAATGG CTACATCAGT GCAGCAGAGC
      961 TTCGCCACGT GATGACAAAC CTTGGAGAGA AGTTAACAGA TGAAGAGGTT GATGAAATGA
     1021 TCAGGGAAGC AGACATCGAT GGGGATGGTC AGGTAAACTA CGAAGAGTTT GTACAAATGA
     1081 TGACAGCGAA GGGCAAGCCG ATCCCTAACC CtCTGCTGGG CCTGGACAGC ACTGGTGGAA
     1141 GTGGATCAGG CAGCGGTGGA TCTTACGGAA GCCATGTGGA TTACGCCGGA GAAAGCttgt
     1201 ttaagggacc acgtgattac aacccgatat cgagcaccat ttgtcatttg acgaatgaat
     1261 ctgatgggca cacaacatcg ttgtatggta ttggatttgg tcccttcatc attacaaaca
     1321 agcacttgtt tAGAAGAaat aatggaacac tgttggtcca atcactacat ggtgtattca
     1381 aggtcaagaa caccacgact ttgcaacaac acctcattga tgggagggac atgataatta
     1441 ttcgcatgcc taaggatttc ccaccatttc ctcaaaagct gaaatttaga gagccacaaa
     1501 gggaagagcg catatgtctt gtgacaacca acttccaaac taagagcatg tctagcatgg
     1561 tgtcagacac tagttgcaca ttcccttcat ctgatggcat attctggaag cattggattc
     1621 aaaccaagga tgggcagtgt ggcagtccat tagtatcaac tagagatggg ttcattgttg
     1681 gtatacactc agcatcgaat ttcaccaaca caaacaatta tttcacaagc gtgccgaaaa
     1741 acttcatgga attgttgaca aatcaggagg cgcagcagtg ggttagtggt tggcgattaa
     1801 atgctgactc agtattgtgg ggAggccata aagttttcat gGTGTAAGAA TTCgatatca
     1861 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     1921 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     1981 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2041 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2101 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2161 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2221 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2281 cttggctgct cgcctAtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2341 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2401 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2461 atcgataccg agcgctgctc gagCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG
     2521 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC
     2581 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA
     2641 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG
     2701 CAGGCATGCT GGGGAggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     2761 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     2821 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     2881 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtattca caccgcatac
     2941 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3001 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3061 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3121 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3181 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3241 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     3301 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3361 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     3421 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3481 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3541 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3601 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3661 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4501 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5461 ctggcctttt gctcacatgt    
//
