LOCUS       pBEST-p70a-UTR3-        3232 bp    DNA     circular UNA 07-MAY-2017
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...z-7j1bori
VERSION     urn.local...z-7j1bori
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     38..59
                     /Sequence="GTGAAGACTATCGCACCATCAG"
                     /Hairpin_Tm="None"
                     /Tm="58.9"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /Pair_Dimer_Tm="0.7"
                     /Product_Size=39
                     /modified_by="User"
                     /label="pBEST seq F"
     promoter        164..218
                     /created_by="User"
                     /Transferred_From="OR2-OR1-Pr"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="p70a promoter"
     3'UTR           236..252
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="UTR3"
     ORF             260..967
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /modified_by="User"
                     /label="ORF 1 (GFP)"
     tag             944..961
                     /created_by="User"
                     /modified_by="User"
                     /label="6xHis"
     terminator      974..1003
                     /created_by="User"
                     /Transferred_From="T500 terminator"
                     /Transferred_Similarity="100.00%"
                     /label="T500"
     misc_feature    complement(1078..1101)
                     /Sequence="GATAAAGAAGACAGTCATAAGTGC"
                     /Hairpin_Tm="None"
                     /Tm="55.6"
                     /Self_Dimer_Tm="None"
                     /%GC="37.5"
                     /modified_by="User"
                     /label="pBEST seq R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1300..1919)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     marker          complement(2074..2934)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     promoter        complement(2976..3004)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
ORIGIN      
        1 agaagacctg accgccgcag agtggatgtt tgacatggtg aagactatcg caccatcagc
       61 cagaaaaccg aattttgctg ggtgggctaa cgatatccgc ctgatgcgtg aacgtgacgg
      121 acgtaaccac cgcgacatgt gtgtgctgtt ccgctgggca tgctgagcta acaccgtgcg
      181 tgttgacaat tttacctctg gcggtgataa tggttgcagc tagcaataat tttgtttaac
      241 tttaagaacg agatatacca tggagctttt cactggcgtt gttcccatcc tggtcgagct
      301 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      361 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      421 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      481 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      541 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      601 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      661 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
      721 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
      781 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
      841 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
      901 ggtcctgctg gagttcgtga ccgccgccgg gatctctaga gtgcaccacc accaccatca
      961 cgtgtaactc gagcaaagcc cgccgaaagg cgggcttttc tgtgtcgacc gatgcccttg
     1021 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     1081 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     1141 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1261 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1501 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1741 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1921 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1981 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2041 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2101 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2161 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2221 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2281 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2341 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2401 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2461 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2521 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2581 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2641 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2701 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2761 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2821 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2881 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2941 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3001 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3061 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3121 gaggcccttt cgtcttcaag aattctggcg aatcctctga ccagccagaa aacgaccttt
     3181 ctgtggtgaa accggatgct gcaattcaga gcggcagcaa gtgggggaca gc
//
