LOCUS       Exported               12210 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJOP-HTT-HR65Q
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Bernice
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 5, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..861
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1908..1929
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1944..1974
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2006..2022
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2141..3997
                     /note="5' homology arms"
     misc_feature    3284..3478
                     /note="CAG tract"
     misc_feature    3512..3528
                     /note="barcode 4 wobble bases"
     misc_feature    4013..4248
                     /note="PB 5'TR"
     misc_feature    4256..4487
                     /note="core insul enhancer"
     promoter        4501..5046
                     /note="Ef1a"
     gene            5063..5815
                     /note="copGFP"
     CDS             5816..5869
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5870..6466
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             6473..6526
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6527..7654
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /note="HSV TK"
                     /translation="ASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQKM
                     PTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQH
                     RLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRH
                     PIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRP
                     GERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRP
                     HIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALL
                     QLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    7703..7824
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    8076..8306
                     /note="core insul"
     misc_feature    8309..8619
                     /note="PB 3'TR"
     misc_feature    8620..11074
                     /note="3' homology arms"
     primer_bind     complement(11140..11156)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      11369..11824
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        12106..12210
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 cagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgcagc ccttcctctt
     2041 tcactgactg actgactgcg tctcaaaacc tcggctgggt caacggaaca ttgtaccaca
     2101 gggagtagtc ccgctgttcg ccctcctatt ctagagctag cgtcacactt ggggtcctca
     2161 ggtcgtgccg accacgcgca ttctctgcgc tctgcgcagg agctcgccca ccctctcccc
     2221 gtgcagagag ccccgcagct ggctccccgc agggctgtcc gggtgagtat ggctctggcc
     2281 acgggccagt gtggcgggag ggcaaacccc aaggccacct cggctcagag tccacggccg
     2341 gctgtcgccc cgctccaggc gtcggcgggg gatcctttcc gcatgggcct gcgcccgcgc
     2401 tcggcgcccc ctccacggcc ccgccccgtc catggccccg tccttcatgg gcgagcccct
     2461 ccatggccct gcccctccgc gccccacccc tccctcgccc cacctctcac cttcctgccc
     2521 cgcccccagc ctccccaccc ctcaccggcc agtcccctcc cctatcccgc tccgcccctc
     2581 agccgccccg cccctcagcc ggcctgccta atgtccccgt ccccagcatc gccccgcccc
     2641 gcccccgtct cgccccgccc ctcaggcggc ctccctgctg tgccccgccc cggcctcgcc
     2701 acgcccctac ctcaccacgc cccccgcatc gccacgcccc ccgcatcgcc acgcctccct
     2761 taccatgcag tcccgccccg tcccttcctc gtcccgcctc gccgcgacac ttcacacaca
     2821 gcttcgcctc accccattac agtctcacca cgccccgtcc cctctccgtt gagccccgcg
     2881 ccttcgcccg ggtggggcgc tgcgctgtca gcggccttgc tgtgtgaggc agaacctgcg
     2941 ggggcagggg cgggctggtt ccctggccag ccattggcag agtccgcagg ctagggctgt
     3001 caatcatgct ggccggcgtg gccccgcctc cgccggcgcg gccccgcctc cgccggcgca
     3061 gcgtctggga cgcaaggcgc cgtgggggct gccgggacgg gtccaagatg gacggccgct
     3121 caggttctgt ttttacctgc ggcccagagc cccattcatt gccccggtgc tgagcggcgc
     3181 cgcgagtcgg cccgaggcct ccggggactg ccgtgccggg cgggagaccg ccatggcgac
     3241 cctggaaaag ctgatgaagg ccttcgagtc cctcaagtcc ttccagcagc agcagcagca
     3301 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     3361 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     3421 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcaacagcc
     3481 gccaccgccg ccgccgccgc cgccgcctcc tcaactgccc caaccgccgc cgcaggcaca
     3541 gccgctgctg cctcagccgc agccgccccc gccgccgccc ccgccgccac ccggcccggc
     3601 tgtggctgag gagccgctgc accgaccgtg agtttgggcc cgctgcagct ccctgtcccg
     3661 gcgggtccca ggctacggcg gggatggcgg taaccctgca gcctgcgggc cggcgacacg
     3721 aacccccggc cccgcagaga cagagtgacc cagcaaccca gagcccatga gggacacccg
     3781 ccccctcctg gggcgaggcc ttcccccact tcagccccgc tccctcactt gggtcttccc
     3841 ttgtcctctc gcgaggggag gcagagcctt gttggggcct gtcctgaatt caccgagggg
     3901 agtcacggcc tcagccctct cgcccttcgc aggatgcgaa gagttggggc gagaacttgt
     3961 ttctttttat ttgcgagaaa ccagggcggg ggttcttgcg gccgcggtct cattaaccct
     4021 agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat tgacgcatgt
     4081 gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt
     4141 atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt
     4201 tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatgaggg
     4261 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     4321 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     4381 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     4441 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaggc ctccaaggcc
     4501 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4561 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     4621 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4681 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4741 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     4801 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     4861 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga cagggccttt gtccggcgct
     4921 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     4981 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     5041 gcctactcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga
     5101 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
     5161 cacccccaag cagggccgca tgaccaacaa gatgaaaagc accaaaggcg ccctgacctt
     5221 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
     5281 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
     5341 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctgcgaggc
     5401 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat
     5461 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
     5521 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag
     5581 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
     5641 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg
     5701 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca
     5761 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg
     5821 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta
     5881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6061 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6241 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc
     6361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccaccg gtgagggcag
     6481 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctgctt cgtaccccgg
     6541 ccatcaacac gcgtctgcgt tcgaccaggc tgcgcgttct cgcggccata gcaaccgacg
     6601 tacggcgttg cgccctcgcc ggcagcaaga agccacggaa gtccgcccgg agcagaaaat
     6661 gcccacgcta ctgcgggttt atatagacgg tccccacggg atggggaaaa ccaccaccac
     6721 gcaactgctg gtggccctgg gttcgcgcga cgatatcgtc tacgtacccg agccgatgac
     6781 ttactggcgg gtgctggggg cttccgagac aatcgcgaac atctacacca cacaacaccg
     6841 cctcgaccag ggtgagatat cggccgggga cgcggcggtg gtaatgacaa gcgcccagat
     6901 aacaatgggc atgccttatg ccgtgaccga cgccgttctg gctcctcata tcggggggga
     6961 ggctgggagc tcacatgccc cgcccccggc cctcaccctc atcttcgacc gccatcccat
     7021 cgccgccctc ctgtgctacc cggccgcgcg gtaccttatg ggcagcatga ccccccaggc
     7081 cgtgctggcg ttcgtggccc tcatcccgcc gaccttgccc ggcaccaaca tcgtgcttgg
     7141 ggcccttccg gaggacagac acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg
     7201 gctggacctg gctatgctgg ctgcgattcg ccgcgtttac gggctacttg ccaatacggt
     7261 gcggtatctg cagtgcggcg ggtcgtggcg ggaggactgg ggacagcttt cggggacggc
     7321 cgtgccgccc cagggtgccg agccccagag caacgcgggc ccacgacccc atatcgggga
     7381 cacgttattt accctgtttc gggcccccga gttgctggcc cccaacggcg acctgtataa
     7441 cgtgtttgcc tgggccttgg acgtcttggc caaacgcctc cgttccatgc acgtctttat
     7501 cctggattac gaccaatcgc ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg
     7561 gatggtccag acccacgtca ccacccccgg ctccataccg acgatatgcg acctggcgcg
     7621 cacgtttgcc cgggagatgg gggaggctaa ctgattttca tcgatttctc gagcttctac
     7681 tgtctggtta gaccgttaac taaacttgtt tattgcagct tataatggtt acaaataaag
     7741 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7801 gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt
     7861 ctatcagaag ctcatctcct tccgggggac aagacatccc tgtttaatat ttaaacagca
     7921 gtgttcccaa actgggttct tatatccctt gctctggtca accaggttgc agggtttcct
     7981 gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg
     8041 actggattcc ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc
     8101 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     8161 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     8221 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     8281 gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat atataataag
     8341 ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa
     8401 gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca
     8461 gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt
     8521 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
     8581 catgcgtcaa ttttacgcag actatctttc tagggttaac tgcgttgtga agagaacttg
     8641 gaggagccga gatttgctca gtgccacttc cctcttctag tctgagaggg aagagggctg
     8701 ggggcgcggg acacttcgag aggaggcggg gtttggagct ggagagatgt gggggcagtg
     8761 gatgacataa tgcttttagg acgcctcggc gggagtggcg gggcgggggg ggggcgggga
     8821 gtgagggcgc gtccaatggg agatttcttt tcctagtggc acttaaaaca gcctgagatt
     8881 tgaggctctt cctacattgt caggacattt catttagttc atgatcacgg tggtagtaac
     8941 acgattttaa gcaccaccta agagatctgc tcatctaagc ctaagttggt ctgcaggcgt
     9001 ttgaatgagt tgtggttgcc aagtaaagtg gtgaacttac gtggtgatta atgaaattat
     9061 cttaaatatt aggaagagtt gattgaagtt ttttgcctat gtgtgttggg aataaaacca
     9121 acacgttgct gatggggagg ttaattgccg agggatgaat gaggtgtaca ttttaccagt
     9181 attccagtca ggcttgccag aatacggggg gtccgcagac tccgtgggca tctcagatgt
     9241 gccagtgaaa gggtttctgt ttgcttcatt gctgacagct tgttactttt tggaagctag
     9301 gggtttctgt tgcttgttct tggggagaat ttttgaaaca ggaaaagaga gaccattaaa
     9361 acatctagcg gaaccccagg actttccctg gaagtctgtg tgtcgagtgt acagtaggag
     9421 ttaggaagta ctctggtgca gttcaggcct ttctcttacc tctcagtatt ctatttccga
     9481 tctggatgtg tcccagatgg catttggtaa gaatatctct gttaagactg attaattttt
     9541 agtaatattt cttgttcttt gtttctgtta tgatccttgt ctcgtcttca aagtttaatt
     9601 agaaaatgat tcggagagca gtgttagctt atttgttgga ataaaattta ggaataaatt
     9661 attctaaagg atggaaaaac tttttggata tttggagaaa ttttaaaaca atttggctta
     9721 tctcttcagt aagtaatttc tcatccagaa atttactgta gtgcttttct aggaggtagg
     9781 tgtcataaaa gttcacacat tgcatgtatc ttgtgtaaac actaaacagg gctcctgatg
     9841 ggaaggaaga cctttctgct gggctgcttc agacacttga tcattctaaa aatatgcctt
     9901 ctctttctta tgctgatttg acagaacctg catttgctta tcttcaaaat atgggtatca
     9961 agaaatttcc tttgctgcct tgacaaagga gatagatttt gtttcattac tttaaggtaa
    10021 tatatgatta ccttatttaa aaaatttaat caggactggc aaggtggctt acacctttaa
    10081 tccgagcact ttgggaggcc taggtggacg aatcacctga ggtcaggagt ttgagaccag
    10141 cctggctaac atggtgaaac cctgtctcta ctaaaaatac aaaaattagc tggtcatggt
    10201 ggcacgtgcc tgtaatccaa gctacctggg aggctgaggc aggaaaatcg cttgaacccg
    10261 ggaggcagag tctgcagtga gttgagatca cgccactgca ctccagcctg ggtgacagag
    10321 cgagactcta tctcaaaaaa aatttttttt aatgtattat ttttgcataa gtaatacatt
    10381 gacatgatac aaattctgta attacaaaag ggcaataatt aaaatatctt ccttccaccc
    10441 ctttcctctg agtacctaac tttgtcccca agaacaagca ctatttcagt tcctcatgta
    10501 tcctgccaga tataacctgt tcatattgta agatagattt aaaatgctct aaaaacaaaa
    10561 gtagtttaga ataatatata tctatatatt ttttgagatg tagtctcaca ttgtcaccca
    10621 ggctggagtg cagtgataca atctcggctc actgcagtct ctgcctccca ggttcaaatg
    10681 cttctcctgc ctcagccttc tgagtagctg ggattacagg cgcccaccac catgtccagc
    10741 taatttttgt atttttagta gagatggggt ttcaccatgt tggccaggct ggtcttgaac
    10801 tcctgacctt gtgatctgtc cacctcggcc tcccaaagtg ctgggattac aggtgtgagc
    10861 caccatgcct ggctagaata ataactttta aaggttctta gcatgctctg aaatcaactg
    10921 cattaggttt atttatagtt ttatagttat tttaaataaa atgcatattt gtcatatttc
    10981 tctgtatttt gctgttgaga aaggaggtat tcactaattt tgagtaacaa acactgctca
    11041 caaagtttgg attttggcag ttctgttcac gtgaactagt tctgaattca ctgactgact
    11101 gactggaaag tcctctccac tgactgtagc ctccaattca ctggccgtcg ttttacaacg
    11161 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    11221 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    11281 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    11341 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
    11401 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    11461 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    11521 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    11581 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    11641 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    11701 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    11761 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
    11821 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
    11881 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
    11941 agacaagctg tgaccgtcaa cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
    12001 aaacgcgcga cccgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
    12061 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
    12121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
    12181 atgcttcaat aatattgaaa aaggaagagt
//
