LOCUS       Exported               12258 bp ds-DNA     circular SYN 04-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJOP-HTT-HR81Q
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12258)
  AUTHORS   Bernice
  TITLE     Direct Submission
  JOURNAL   Exported Friday, May 5, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..861
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1908..1929
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1944..1974
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2006..2022
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2141..4045
                     /note="5' homology arms"
     misc_feature    3284..3526
                     /note="CAG tract"
     misc_feature    3560..3576
                     /note="barcode 4 wobble bases"
     misc_feature    4061..4296
                     /note="PB 5'TR"
     misc_feature    4304..4535
                     /note="core insul enhancer"
     promoter        4549..5094
                     /note="Ef1a"
     gene            5111..5863
                     /note="copGFP"
     CDS             5864..5917
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5918..6514
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             6521..6574
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6575..7702
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /note="HSV TK"
                     /translation="ASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQKM
                     PTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQH
                     RLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRH
                     PIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRP
                     GERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRP
                     HIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALL
                     QLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    7751..7872
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    8124..8354
                     /note="core insul"
     misc_feature    8357..8667
                     /note="PB 3'TR"
     misc_feature    8668..11122
                     /note="3' homology arms"
     primer_bind     complement(11188..11204)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      11417..11872
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        12154..12258
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 cagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgcagc ccttcctctt
     2041 tcactgactg actgactgcg tctcaaaacc tcggctgggt caacggaaca ttgtaccaca
     2101 gggagtagtc ccgctgttcg ccctcctatt ctagagctag cgtcacactt ggggtcctca
     2161 ggtcgtgccg accacgcgca ttctctgcgc tctgcgcagg agctcgccca ccctctcccc
     2221 gtgcagagag ccccgcagct ggctccccgc agggctgtcc gggtgagtat ggctctggcc
     2281 acgggccagt gtggcgggag ggcaaacccc aaggccacct cggctcagag tccacggccg
     2341 gctgtcgccc cgctccaggc gtcggcgggg gatcctttcc gcatgggcct gcgcccgcgc
     2401 tcggcgcccc ctccacggcc ccgccccgtc catggccccg tccttcatgg gcgagcccct
     2461 ccatggccct gcccctccgc gccccacccc tccctcgccc cacctctcac cttcctgccc
     2521 cgcccccagc ctccccaccc ctcaccggcc agtcccctcc cctatcccgc tccgcccctc
     2581 agccgccccg cccctcagcc ggcctgccta atgtccccgt ccccagcatc gccccgcccc
     2641 gcccccgtct cgccccgccc ctcaggcggc ctccctgctg tgccccgccc cggcctcgcc
     2701 acgcccctac ctcaccacgc cccccgcatc gccacgcccc ccgcatcgcc acgcctccct
     2761 taccatgcag tcccgccccg tcccttcctc gtcccgcctc gccgcgacac ttcacacaca
     2821 gcttcgcctc accccattac agtctcacca cgccccgtcc cctctccgtt gagccccgcg
     2881 ccttcgcccg ggtggggcgc tgcgctgtca gcggccttgc tgtgtgaggc agaacctgcg
     2941 ggggcagggg cgggctggtt ccctggccag ccattggcag agtccgcagg ctagggctgt
     3001 caatcatgct ggccggcgtg gccccgcctc cgccggcgcg gccccgcctc cgccggcgca
     3061 gcgtctggga cgcaaggcgc cgtgggggct gccgggacgg gtccaagatg gacggccgct
     3121 caggttctgt ttttacctgc ggcccagagc cccattcatt gccccggtgc tgagcggcgc
     3181 cgcgagtcgg cccgaggcct ccggggactg ccgtgccggg cgggagaccg ccatggcgac
     3241 cctggaaaag ctgatgaagg ccttcgagtc cctcaagtcc ttccagcagc agcagcagca
     3301 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     3361 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     3421 gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca
     3481 gcagcagcag cagcagcagc agcagcagca gcagcagcag caacagccgc caccgccgcc
     3541 gccgccgccg ccgcctcctc acctgcccca cccgccgccg caggcacagc cgctgctgcc
     3601 tcagccgcag ccgcccccgc cgccgccccc gccgccaccc ggcccggctg tggctgagga
     3661 gccgctgcac cgaccgtgag tttgggcccg ctgcagctcc ctgtcccggc gggtcccagg
     3721 ctacggcggg gatggcggta accctgcagc ctgcgggccg gcgacacgaa cccccggccc
     3781 cgcagagaca gagtgaccca gcaacccaga gcccatgagg gacacccgcc ccctcctggg
     3841 gcgaggcctt cccccacttc agccccgctc cctcacttgg gtcttccctt gtcctctcgc
     3901 gaggggaggc agagccttgt tggggcctgt cctgaattca ccgaggggag tcacggcctc
     3961 agccctctcg cccttcgcag gatgcgaaga gttggggcga gaacttgttt ctttttattt
     4021 gcgagaaacc agggcggggg ttcttgcggc cgcggtctca ttaaccctag aaagataatc
     4081 atattgtgac gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt tttatcggtc
     4141 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     4201 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     4261 acaaaaactc aaaatttctt ctataaagta acaaaacttt tatgagggac agcccccccc
     4321 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     4381 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     4441 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     4501 tgagcctgca gacacctggg gggatacggg gaaaaggcct ccaaggccaa ggatctgcga
     4561 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     4621 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     4681 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4741 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     4801 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     4861 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     4921 ctaggtaagt ttaaagctca ggtcgagaca gggcctttgt ccggcgctcc cttggagcct
     4981 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     5041 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctactctaga
     5101 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     5161 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     5221 gggccgcatg accaacaaga tgaaaagcac caaaggcgcc ctgaccttca gcccctacct
     5281 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     5341 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     5401 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tgcgaggccg gccgcgtgat
     5461 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     5521 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     5581 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     5641 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     5701 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     5761 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc
     5821 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct
     5881 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     5941 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6001 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6061 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6121 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6181 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6241 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6301 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6361 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct
     6421 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6481 gcgcacctgg tgcatgaccc gcaagcccgg tgccaccggt gagggcagag gaagtcttct
     6541 aacatgcggt gacgtggagg agaatcccgg ccctgcttcg taccccggcc atcaacacgc
     6601 gtctgcgttc gaccaggctg cgcgttctcg cggccatagc aaccgacgta cggcgttgcg
     6661 ccctcgccgg cagcaagaag ccacggaagt ccgcccggag cagaaaatgc ccacgctact
     6721 gcgggtttat atagacggtc cccacgggat ggggaaaacc accaccacgc aactgctggt
     6781 ggccctgggt tcgcgcgacg atatcgtcta cgtacccgag ccgatgactt actggcgggt
     6841 gctgggggct tccgagacaa tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg
     6901 tgagatatcg gccggggacg cggcggtggt aatgacaagc gcccagataa caatgggcat
     6961 gccttatgcc gtgaccgacg ccgttctggc tcctcatatc gggggggagg ctgggagctc
     7021 acatgccccg cccccggccc tcaccctcat cttcgaccgc catcccatcg ccgccctcct
     7081 gtgctacccg gccgcgcggt accttatggg cagcatgacc ccccaggccg tgctggcgtt
     7141 cgtggccctc atcccgccga ccttgcccgg caccaacatc gtgcttgggg cccttccgga
     7201 ggacagacac atcgaccgcc tggccaaacg ccagcgcccc ggcgagcggc tggacctggc
     7261 tatgctggct gcgattcgcc gcgtttacgg gctacttgcc aatacggtgc ggtatctgca
     7321 gtgcggcggg tcgtggcggg aggactgggg acagctttcg gggacggccg tgccgcccca
     7381 gggtgccgag ccccagagca acgcgggccc acgaccccat atcggggaca cgttatttac
     7441 cctgtttcgg gcccccgagt tgctggcccc caacggcgac ctgtataacg tgtttgcctg
     7501 ggccttggac gtcttggcca aacgcctccg ttccatgcac gtctttatcc tggattacga
     7561 ccaatcgccc gccggctgcc gggacgccct gctgcaactt acctccggga tggtccagac
     7621 ccacgtcacc acccccggct ccataccgac gatatgcgac ctggcgcgca cgtttgcccg
     7681 ggagatgggg gaggctaact gattttcatc gatttctcga gcttctactg tctggttaga
     7741 ccgttaacta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7861 caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct
     7921 catctccttc cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac
     7981 tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga
     8041 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt
     8101 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     8161 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     8221 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     8281 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     8341 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     8401 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     8461 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     8521 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     8581 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     8641 ttacgcagac tatctttcta gggttaactg cgttgtgaag agaacttgga ggagccgaga
     8701 tttgctcagt gccacttccc tcttctagtc tgagagggaa gagggctggg ggcgcgggac
     8761 acttcgagag gaggcggggt ttggagctgg agagatgtgg gggcagtgga tgacataatg
     8821 cttttaggac gcctcggcgg gagtggcggg gcgggggggg ggcggggagt gagggcgcgt
     8881 ccaatgggag atttcttttc ctagtggcac ttaaaacagc ctgagatttg aggctcttcc
     8941 tacattgtca ggacatttca tttagttcat gatcacggtg gtagtaacac gattttaagc
     9001 accacctaag agatctgctc atctaagcct aagttggtct gcaggcgttt gaatgagttg
     9061 tggttgccaa gtaaagtggt gaacttacgt ggtgattaat gaaattatct taaatattag
     9121 gaagagttga ttgaagtttt ttgcctatgt gtgttgggaa taaaaccaac acgttgctga
     9181 tggggaggtt aattgccgag ggatgaatga ggtgtacatt ttaccagtat tccagtcagg
     9241 cttgccagaa tacggggggt ccgcagactc cgtgggcatc tcagatgtgc cagtgaaagg
     9301 gtttctgttt gcttcattgc tgacagcttg ttactttttg gaagctaggg gtttctgttg
     9361 cttgttcttg gggagaattt ttgaaacagg aaaagagaga ccattaaaac atctagcgga
     9421 accccaggac tttccctgga agtctgtgtg tcgagtgtac agtaggagtt aggaagtact
     9481 ctggtgcagt tcaggccttt ctcttacctc tcagtattct atttccgatc tggatgtgtc
     9541 ccagatggca tttggtaaga atatctctgt taagactgat taatttttag taatatttct
     9601 tgttctttgt ttctgttatg atccttgtct cgtcttcaaa gtttaattag aaaatgattc
     9661 ggagagcagt gttagcttat ttgttggaat aaaatttagg aataaattat tctaaaggat
     9721 ggaaaaactt tttggatatt tggagaaatt ttaaaacaat ttggcttatc tcttcagtaa
     9781 gtaatttctc atccagaaat ttactgtagt gcttttctag gaggtaggtg tcataaaagt
     9841 tcacacattg catgtatctt gtgtaaacac taaacagggc tcctgatggg aaggaagacc
     9901 tttctgctgg gctgcttcag acacttgatc attctaaaaa tatgccttct ctttcttatg
     9961 ctgatttgac agaacctgca tttgcttatc ttcaaaatat gggtatcaag aaatttcctt
    10021 tgctgccttg acaaaggaga tagattttgt ttcattactt taaggtaata tatgattacc
    10081 ttatttaaaa aatttaatca ggactggcaa ggtggcttac acctttaatc cgagcacttt
    10141 gggaggccta ggtggacgaa tcacctgagg tcaggagttt gagaccagcc tggctaacat
    10201 ggtgaaaccc tgtctctact aaaaatacaa aaattagctg gtcatggtgg cacgtgcctg
    10261 taatccaagc tacctgggag gctgaggcag gaaaatcgct tgaacccggg aggcagagtc
    10321 tgcagtgagt tgagatcacg ccactgcact ccagcctggg tgacagagcg agactctatc
    10381 tcaaaaaaaa ttttttttaa tgtattattt ttgcataagt aatacattga catgatacaa
    10441 attctgtaat tacaaaaggg caataattaa aatatcttcc ttccacccct ttcctctgag
    10501 tacctaactt tgtccccaag aacaagcact atttcagttc ctcatgtatc ctgccagata
    10561 taacctgttc atattgtaag atagatttaa aatgctctaa aaacaaaagt agtttagaat
    10621 aatatatatc tatatatttt ttgagatgta gtctcacatt gtcacccagg ctggagtgca
    10681 gtgatacaat ctcggctcac tgcagtctct gcctcccagg ttcaaatgct tctcctgcct
    10741 cagccttctg agtagctggg attacaggcg cccaccacca tgtccagcta atttttgtat
    10801 ttttagtaga gatggggttt caccatgttg gccaggctgg tcttgaactc ctgaccttgt
    10861 gatctgtcca cctcggcctc ccaaagtgct gggattacag gtgtgagcca ccatgcctgg
    10921 ctagaataat aacttttaaa ggttcttagc atgctctgaa atcaactgca ttaggtttat
    10981 ttatagtttt atagttattt taaataaaat gcatatttgt catatttctc tgtattttgc
    11041 tgttgagaaa ggaggtattc actaattttg agtaacaaac actgctcaca aagtttggat
    11101 tttggcagtt ctgttcacgt gaactagttc tgaattcact gactgactga ctggaaagtc
    11161 ctctccactg actgtagcct ccaattcact ggccgtcgtt ttacaacgtc gtgactggga
    11221 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11281 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11341 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
    11401 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    11521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    11581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
    11641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    11701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
    11761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    11821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
    11881 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
    11941 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    12001 accgtcaacg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgacc
    12061 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
    12121 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    12181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    12241 tattgaaaaa ggaagagt
//
