LOCUS       Exported                6337 bp ds-DNA     circular SYN 14-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJL006
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jun 14, 2017 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        80..124
                     /note="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    100..116
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    132..148
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             191..1339
                     /codon_start=1
                     /label=FtsZ
                     /note="FtsZD212A"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNTD
                     AQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAAGM
                     GGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSLITI
                     PNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFAAVRTVMSEMGY
                     AMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFETVGNT
                     IRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQPVMDRYQ
                     QHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     misc_feature    1340..1375
                     /label=gflinker
                     /note="gflinker"
     CDS             1376..2056
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /note="mEos3.2"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     terminator      2105..2199
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    2107..2130
                     /note="RM119 R-primer"
     promoter        2755..2857
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2858..3517
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4066..4654
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4898..4975
                     /gene="lacI (mutant)"
                     /note="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4976..6058
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /note="lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        6107..6137
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6145..6161
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6169..6185
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6201..6217)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6291..6311
                     /note="RM118 F-primer"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactactagt atgtttgaac caatggaact taccaatgac gcggtgatta aagtcatcgg
      241 cgtcggcggc ggcggcggta atgctgttga acacatggtg cgcgagcgca ttgaaggtgt
      301 tgaattcttc gcggtaaata ccgatgcaca agcgctgcgt aaaacagcgg ttggacagac
      361 gattcaaatc ggtagcggta tcaccaaagg actgggcgct ggcgctaatc cagaagttgg
      421 ccgcaatgcg gctgatgagg atcgcgatgc attgcgtgcg gcgctggaag gtgcagacat
      481 ggtctttatt gctgcgggta tgggtggtgg taccggtaca ggtgcagcac cagtcgtcgc
      541 tgaagtggca aaagatttgg gtatcctgac cgttgctgtc gtcactaagc ctttcaactt
      601 tgaaggcaag aagcgtatgg cattcgcgga gcaggggatc actgaactgt ccaagcatgt
      661 ggactctctg atcactatcc cgaacgacaa actgctgaaa gttctgggcc gcggtatctc
      721 cctgctggat gcgtttggcg cagcgaacga tgtactgaaa ggcgctgtgc aaggtatcgc
      781 tgaactgatt actcgtccgg gtttgatgaa cgtggacttt gcagccgtac gcaccgtaat
      841 gtctgagatg ggctacgcaa tgatgggttc tggcgtggcg agcggtgaag accgtgcgga
      901 agaagctgct gaaatggcta tctcttctcc gctgctggaa gatatcgacc tgtctggcgc
      961 gcgcggcgtg ctggttaaca tcacggcggg cttcgatctg cgtctggatg agttcgaaac
     1021 ggtaggtaac accatccgtg catttgcttc cgacaacgcg actgtggtta tcggtacttc
     1081 tcttgacccg gatatgaatg acgagctgcg cgtaaccgtt gttgcgacag gtatcggcat
     1141 ggacaaacgt cctgaaatca ctctggtgac caataagcag gttcagcagc cagtgatgga
     1201 tcgctaccag cagcatggga tggctccgct gacccaggag cagaagccgg ttgctaaagt
     1261 cgtgaatgac aatgcgccgc aaactgcgaa agagccggat tatctggata tcccagcatt
     1321 cctgcgtaag caagctgatg gatccgctgg ctccgctgct ggttctggcg aattcatgag
     1381 tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca
     1441 ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct
     1501 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
     1561 ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc
     1621 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcaa
     1681 tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gattttatgg
     1741 taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
     1801 cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattgaaa tggctttgtt
     1861 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
     1921 gggtgtcaag ttaccaggcg cacactttgt ggaccactgc attgagattt taagccatga
     1981 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
     2041 caatgccaga cgataagcgg ccgctaaggg tcgacctgca gccaagctta attagctgag
     2101 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     2161 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagctaggg gaattcatcg
     2221 tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2281 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     2341 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     2401 cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     2461 ggttaatgtc atgataataa tggtttctta ggatcctgat gtccggcggt gcttttgccg
     2521 ttacgcacca ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact
     2581 ggagcacctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccgacgc
     2641 actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg
     2701 tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg
     2761 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     2821 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     2881 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     2941 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     3001 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     3061 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     3121 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     3181 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     3241 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     3301 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     3361 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     3421 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     3481 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     3541 cccttaaacg cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     3601 tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg
     3661 tctattgctg gtttaccggt ttattgacta ccggaagcag tgtgaccgtg tgcttctcaa
     3721 atgcctgagg ccagtttgct caggctctcc ccgtggaggt aataattgac gatatgatca
     3781 tttattctgc ctcccagagc ctgataaaaa cggttaggat cggagtcagg caactatgga
     3841 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     3901 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3961 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4141 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4201 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4261 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4321 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4381 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4441 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4501 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4561 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4621 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4681 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4741 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4801 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4861 tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat ggtgcaaaac
     4921 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
     4981 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
     5041 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
     5101 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
     5161 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
     5221 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
     5281 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
     5341 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
     5401 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
     5461 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
     5521 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
     5581 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
     5641 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
     5701 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     5761 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     5821 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     5881 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     5941 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     6001 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6061 gcaacgcaat taatgtaagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6181 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6241 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6301 gaagaggccc gcaccgatcg cccttcccaa cagttgc
//
