LOCUS       pHdzCas9-KRAB          11169 bp    DNA     circular     11-AUG-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11365510|
COMMENT     VNTDBDATE|-11365510|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pHdzCas9-KRAB|
COMMENT     VNTAUTHORNAME|Kao Pei Hsun|
FEATURES             Location/Qualifiers
     promoter        112..866
                     /vntifkey="29"
                     /label=CaMV35S\promoter
     3'UTR           5728..5977
                     /vntifkey="50"
                     /label=nos\3'UTR
     promoter        6022..6802
                     /vntifkey="29"
                     /label=CaMV35S\promoter
     CDS             6838..7863
                     /vntifkey="4"
                     /label=HygR
     3'UTR           7879..8087
                     /vntifkey="50"
                     /label=CaMV35S\3'UTR
     rep_origin      9439..10058
                     /vntifkey="33"
                     /label=pBR322\ori
     CDS             complement(10132..10941)
                     /vntifkey="4"
                     /label=KanR
     CDS             1047..5678
                     /vntifkey="4"
                     /label=dzCas9-KRAB
     CDS             1047..5315
                     /vntifkey="4"
                     /label=dzCas9
BASE COUNT     2982 a      2839 c      2899 g      2449 t 
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 
       61 aatctgatcc aagctcaagc taagctcacg tgacggaatt aagcttgcat gcctgcaggt 
      121 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga 
      181 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt 
      241 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta 
      301 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg 
      361 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac 
      421 gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac ttgtctactc 
      481 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag 
      541 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa 
      601 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat 
      661 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 
      721 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc 
      781 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata 
      841 aggaagttca tttcatttgg agaggacctc gacctcaaca caacatatac aaaacaaacg 
      901 aatctcaagc aatcaagcat tctacttcta ttgcagcaat ttaaatcatt tcttttaaag 
      961 caaaagcaat tttctgaaaa ttttcaccat ttacgaacga tactcgaggg ggatccccaa 
     1021 tacttgtatg gccgcggccg ctctagatgg attacaagga ccacgacggg gattacaagg 
     1081 accacgacat tgattacaag gatgatgatg acaagatggc tccgaagaag aagaggaagg 
     1141 ttggcatcca cggggtgcca gctgctgaca agaagtactc gatcggcctc gccattggga 
     1201 ctaactctgt tggctgggcc gtgatcaccg acgagtacaa ggtgccctca aagaagttca 
     1261 aggtcctggg caacaccgat cggcattcca tcaagaagaa tctcattggc gctctcctgt 
     1321 tcgacagcgg cgagacggct gaggctacgc ggctcaagcg caccgcccgc aggcggtaca 
     1381 cgcgcaggaa gaatcgcatc tgctacctgc aggagatttt ctccaacgag atggcgaagg 
     1441 ttgacgattc tttcttccac aggctggagg agtcattcct cgtggaggag gataagaagc 
     1501 acgagcggca tccaatcttc ggcaacattg tcgacgaggt tgcctaccac gagaagtacc 
     1561 ctacgatcta ccatctgcgg aagaagctcg tggactccac agataaggcg gacctccgcc 
     1621 tgatctacct cgctctggcc cacatgatta agttcagggg ccatttcctg atcgaggggg 
     1681 atctcaaccc ggacaatagc gatgttgaca agctgttcat ccagctcgtg cagacgtaca 
     1741 accagctctt cgaggagaac cccattaatg cgtcaggcgt cgacgcgaag gctatcctgt 
     1801 ccgctaggct ctcgaagtct cggcgcctcg agaacctgat cgcccagctg ccgggcgaga 
     1861 agaagaacgg cctgttcggg aatctcattg cgctcagcct ggggctcacg cccaacttca 
     1921 agtcgaattt cgatctcgct gaggacgcca agctgcagct ctccaaggac acatacgacg 
     1981 atgacctgga taacctcctg gcccagatcg gcgatcagta cgcggacctg ttcctcgctg 
     2041 ccaagaatct gtcggacgcc atcctcctgt ctgatattct cagggtgaac accgagatta 
     2101 cgaaggctcc gctctcagcc tccatgatca agcgctacga cgagcaccat caggatctga 
     2161 ccctcctgaa ggcgctggtc aggcagcagc tccccgagaa gtacaaggag atcttcttcg 
     2221 atcagtcgaa gaacggctac gctgggtaca ttgacggcgg ggcctctcag gaggagttct 
     2281 acaagttcat caagccgatt ctggagaaga tggacggcac ggaggagctg ctggtgaagc 
     2341 tcaatcgcga ggacctcctg aggaagcagc ggacattcga taacggcagc atcccacacc 
     2401 agattcatct cggggagctg cacgctatcc tgaggaggca ggaggacttc taccctttcc 
     2461 tcaaggataa ccgcgagaag atcgagaaga ttctgacttt caggatcccg tactacgtcg 
     2521 gcccactcgc taggggcaac tcccgcttcg cttggatgac ccgcaagtca gaggagacga 
     2581 tcacgccgtg gaacttcgag gaggtggtcg acaagggcgc tagcgctcag tcgttcatcg 
     2641 agaggatgac gaatttcgac aagaacctgc caaatgagaa ggtgctccct aagcactcgc 
     2701 tcctgtacga gtacttcaca gtctacaacg agctgactaa ggtgaagtat gtgaccgagg 
     2761 gcatgaggaa gccggctttc ctgtctgggg agcagaagaa ggccatcgtg gacctcctgt 
     2821 tcaagaccaa ccggaaggtc acggttaagc agctcaagga ggactacttc aagaagattg 
     2881 agtgcttcga ttcggtcgag atctctggcg ttgaggaccg cttcaacgcc tccctgggga 
     2941 cctaccacga tctcctgaag atcattaagg ataaggactt cctggacaac gaggagaatg 
     3001 aggatatcct cgaggacatt gtgctgacac tcactctgtt cgaggaccgg gagatgatcg 
     3061 aggagcgcct gaagacttac gcccatctct tcgatgacaa ggtcatgaag cagctcaaga 
     3121 ggaggaggta caccggctgg gggaggctga gcaggaagct catcaacggc attcgggaca 
     3181 agcagtccgg gaagacgatc ctcgacttcc tgaagagcga tggcttcgcg aaccgcaatt 
     3241 tcatgcagct gattcacgat gacagcctca cattcaagga ggatatccag aaggctcagg 
     3301 tgagcggcca gggggactcg ctgcacgagc atatcgcgaa cctcgctggc tcgccagcta 
     3361 tcaagaaggg gattctgcag accgtgaagg ttgtggacga gctggtgaag gtcatgggca 
     3421 ggcacaagcc tgagaacatc gtcattgaga tggcccggga gaatcagacc acgcagaagg 
     3481 gccagaagaa ctcacgcgag aggatgaaga ggatcgagga gggcattaag gagctggggt 
     3541 cccagatcct caaggagcac ccggtggaga acacgcagct gcagaatgag aagctctacc 
     3601 tgtactacct ccagaatggc cgcgatatgt atgtggacca ggagctggat attaacaggc 
     3661 tcagcgatta cgacgtcgat gccatcgttc cacagtcatt cctgaaggat gactccattg 
     3721 acaacaaggt cctcaccagg tcggacaaga accggggcaa gtctgataat gttccttcag 
     3781 aggaggtcgt taagaagatg aagaactact ggcgccagct cctgaatgcc aagctgatca 
     3841 cgcagcggaa gttcgataac ctcacaaagg ctgagagggg cgggctctct gagctggaca 
     3901 aggcgggctt catcaagagg cagctggtcg agacacggca gatcactaag cacgttgcgc 
     3961 agattctcga ctcacggatg aacactaagt acgatgagaa tgacaagctg atccgcgagg 
     4021 tgaaggtcat caccctgaag tcaaagctcg tctccgactt caggaaggat ttccagttct 
     4081 acaaggttcg ggagatcaac aattaccacc atgcccatga cgcgtacctg aacgcggtgg 
     4141 tcggcacagc tctgatcaag aagtacccaa agctcgagag cgagttcgtg tacggggact 
     4201 acaaggttta cgatgtgagg aagatgatcg ccaagtcgga gcaggagatt ggcaaggcta 
     4261 ccgccaagta cttcttctac tctaacatta tgaatttctt caagacagag atcactctgg 
     4321 ccaatggcga gatccggaag cgccccctca tcgagacgaa cggcgagacg ggggagatcg 
     4381 tgtgggacaa gggcagggat ttcgcgaccg tcaggaaggt tctctccatg ccacaagtga 
     4441 atatcgtcaa gaagacagag gtccagactg gcgggttctc taaggagtca attctgccta 
     4501 agcggaacag cgacaagctc atcgcccgca agaaggactg ggatccgaag aagtacggcg 
     4561 ggttcgacag ccccactgtg gcctactcgg tcctggttgt ggcgaaggtt gagaagggca 
     4621 agtccaagaa gctcaagagc gtgaaggagc tgctggggat cacgattatg gagcgctcca 
     4681 gcttcgagaa gaacccgatc gatttcctgg aggcgaaggg ctacaaggag gtgaagaagg 
     4741 acctgatcat taagctcccc aagtactcac tcttcgagct ggagaacggc aggaagcgga 
     4801 tgctggcttc cgctggcgag ctgcagaagg ggaacgagct ggctctgccg tccaagtatg 
     4861 tgaacttcct ctacctggcc tcccactacg agaagctcaa gggcagcccc gaggacaacg 
     4921 agcagaagca gctgttcgtc gagcagcaca agcattacct cgacgagatc attgagcaga 
     4981 tttccgagtt ctccaagcgc gtgatcctgg ccgacgcgaa tctggataag gtcctctccg 
     5041 cgtacaacaa gcaccgcgac aagccaatca gggagcaggc tgagaatatc attcatctct 
     5101 tcaccctgac gaacctcggc gcccctgctg ctttcaagta cttcgacaca actatcgatc 
     5161 gcaagaggta cacaagcact aaggaggtcc tggacgcgac cctcatccac cagtcgatta 
     5221 ccggcctcta cgagacgcgc atcgacctgt ctcagctcgg gggcgacaag cggccagcgg 
     5281 cgacgaagaa ggcggggcag gcgaagaaga agaagtccgg gtcctcgggg tcgccaaaga 
     5341 agaagcggaa ggtcggctca agcgggtcac caaagaagaa gcggaaggtc ggctcgatgg 
     5401 acgctaagtc cctcaccgct tggagccgca ccctggtgac gttcaaggac gtgttcgtcg 
     5461 atttcacaag ggaggagtgg aagctcctgg acactgctca gcagatcgtt taccggaacg 
     5521 tgatgctgga gaactacaag aacctcgtgt ccctcggcta ccagctgacg aagccggatg 
     5581 ttattctccg cctggagaag ggcgaggagc ccgggtcccc taagaagaag cggaaggtcg 
     5641 gctcctaccc atacgatgtc cctgactacg cctcatgagc tcaggcctcc atacaagtat 
     5701 tggggatcca ctacgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga 
     5761 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag 
     5821 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga 
     5881 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat 
     5941 aaattatcgc gcgcggtgtc atctatgtta ctagatcgga attcgaggcg gtttgcgtat 
     6001 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc 
     6061 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg 
     6121 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa 
     6181 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat 
     6241 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa 
     6301 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac 
     6361 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt 
     6421 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc 
     6481 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc 
     6541 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc 
     6601 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg 
     6661 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa 
     6721 gaccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt 
     6781 ctctctctac aaatctatct ctctcgagct ttcgcagatc ccggggggca atgagatatg 
     6841 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc 
     6901 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta 
     6961 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt 
     7021 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg 
     7081 gagtttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa 
     7141 gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg 
     7201 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc 
     7261 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac 
     7321 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg 
     7381 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc 
     7441 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg 
     7501 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt 
     7561 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga 
     7621 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc 
     7681 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc 
     7741 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt 
     7801 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa 
     7861 tagagtagat gccgaccgga tctgtcgatc gacaagctcg agtttctcca taataatgtg 
     7921 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca 
     7981 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta 
     8041 attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat 
     8101 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt cactagtcag gttaactcaa 
     8161 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 
     8221 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 
     8281 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 
     8341 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 
     8401 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 
     8461 cgatgacaga gcgttgctgc ctgtgatcac ttaagtaact aactaacagg aagagtttgt 
     8521 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt 
     8581 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct 
     8641 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag 
     8701 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc 
     8761 ttagtagtta gacgtccccg agatccatgc tagaccatga atccagaagc ccgagaggtt 
     8821 gccgcctttc gggctttttc tttttcaaaa aaaaaaattt ataaaacgat ctgttgcggc 
     8881 cggccgccgg gttgtgggca aaggcgctcg acggtgggca accgcttgcg gttgtccacg 
     8941 ggcggagccg gtgcgcgtag cgcattgtcc acaagccaag ggcgaccaat aattgatata 
     9001 tatattcata attgaaaagc taattgaaca tactacttgc tgtaactact tgccggagcg 
     9061 aggggtgttt gcaagctgtt gatctgaaag ggctattagc gttctcacgt gcctttttga 
     9121 ttagcgattt cacgtgacct tattagcgat ttcacgtact ccgattagcg atttcacgta 
     9181 ccctgattag cgatttcacg tggatagttt ttggagcggg ccggaaagcc ccgtgaatca 
     9241 aggctttgcg gggcattagc ggtttcacgt ggataactac cctctatcca caggcttccg 
     9301 gggataaaaa agcccgctcg acggcgggct gttggatggg gatctagcgg taatacggtt 
     9361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     9421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     9481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     9541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     9601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     9661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     9721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     9781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     9841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 
     9901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     9961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
    10021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctaccgggt ctgacgctca 
    10081 gtggaacggg gcccaatctg aataatgtta caaccaatta accaattctg attagaaaaa 
    10141 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt 
    10201 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc 
    10261 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt 
    10321 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg 
    10381 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg 
    10441 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc 
    10501 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg 
    10561 gcgcagggac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa 
    10621 tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt 
    10681 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac 
    10741 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg 
    10801 cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg 
    10861 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt 
    10921 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt 
    10981 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac 
    11041 gggccagagc tgcagtttga tcccgagggg aaccctgtgg ttgacatgca catacaaatg 
    11101 gacgaacgga taaacctttt cacgcccttt taaatatccg ttattctaat aaacgctctt 
    11161 ttctcttag 
//
