LOCUS       pIKU801::CrPEPC1        4691 bp    DNA     circular      6-DEC-2016
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11351478|
COMMENT     VNTDBDATE|-11533881|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pIKU801::CrPEPC1|
COMMENT     VNTAUTHORNAME|Kao Pei Hsun|
FEATURES             Location/Qualifiers
     CDS             1445..2248
                     /vntifkey="4"
                     /label=aphVIII\(ParoR)
     rep_origin      complement(2999..3618)
                     /vntifkey="33"
                     /label=pBR322
     CDS             complement(3774..4634)
                     /vntifkey="4"
                     /label=AmpR
     promoter        complement(4635..4686)
                     /vntifkey="30"
                     /label=Pamp
     3'UTR           2322..2555
                     /vntifkey="50"
                     /label=RbcS2-3'UTR
     promoter        754..1249
                     /vntifkey="29"
                     /label=Hsp70A-RbcS2\promoter
     intron          1258..1402
                     /vntifkey="15"
                     /label=RbcS2-intron
     promoter        19..442
                     /vntifkey="29"
                     /label=AtU6-26\promoter
     misc_feature    464..540
                     /vntifkey="21"
                     /label=sgRNA-scaffold
     3'UTR           540..731
                     /vntifkey="50"
                     /label=AtU6-26\TT
     misc_feature    444..463
                     /vntifkey="21"
                     /label=Target\(CrPEPC1)
     primer          11..40
                     /vntifkey="27"
                     /label=XhoI-U6P-F
     primer          complement(707..739)
                     /vntifkey="27"
                     /label=EcoRI-U6T-R
     primer          complement(422..470)
                     /vntifkey="27"
                     /label=CrPEPC1(N20)-U6P-R
     primer          444..488
                     /vntifkey="27"
                     /label=CrPEPC1(N20)-sgRNA-F
BASE COUNT     1024 a      1143 c      1315 g      1209 t 
ORIGIN
        1 ctgcagagat ctctcgagcg acttgccttc cgcacaatac atcatttctt cttagctttt 
       61 tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg 
      121 tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt 
      181 ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat 
      241 cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga 
      301 agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg 
      361 aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac 
      421 agctagagtc gaagtagtga ttgggacgcg gtgaccacct accgttttag agctagaaat 
      481 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct 
      541 tttttttgca aaattttcca gatcgatttc ttcttcctct gttcttcggc gttcaatttc 
      601 tggggttttc tcttcgtttt ctgtaactga aacctaaaat ttgacctaaa aaaaatctca 
      661 aataatatga ttcagtggtt ttgtactttt cagttagttg agttttgcag ttccgatgag 
      721 ataaaccaat agaattcacg cgtcatatgg agctcgctga ggcttgacat gattggtgcg 
      781 tatgtttgta tgaagctaca ggactgattt ggcgggctat gagggcgcgg gaagctctgg 
      841 aagggccgcg atggggcgcg cggcgtccag aaggcgccat acggcccgct ggcggcaccc 
      901 atccggtata aaagcccgcg accccgaacg gtgacctcca ctttcagcga caaacgagca 
      961 cttatacata cgcgactatt ctgccgctat acataaccac tcagctagct taagatccca 
     1021 tcaagcttgc atgccgggcg cgccagaagg agcgcagcca aaccaggatg atgtttgatg 
     1081 gggtatttga gcacttgcaa cccttatccg gaagccccct ggcccacaaa ggctaggcgc 
     1141 caatgcaagc agttcgcatg cagcccctgg agcggtgccc tcctgataaa ccggccaggg 
     1201 ggcctatgtt ctttactttt ttacaagaga agtcactcaa catcttaaaa tggccaggtg 
     1261 agtcgacgag caagcccggc ggatcaggca gcgtgcttgc agatttgact tgcaacgccc 
     1321 gcattgtgtc gacgaaggct tttggctcct ctgtcgctgt ctcaagcagc atctaaccct 
     1381 gcgtcgccgt ttccatttgc aggatggcca ctccgccctc cccggtgctg aagaatttcg 
     1441 aagcatggac gatgcgttgc gtgcactgcg gggtcggtat cccggttgtg agtgggttgt 
     1501 tgtggaggat ggggcctcgg gggctggtgt ttatcggctt cggggtggtg ggcgggagtt 
     1561 gtttgtcaag gtggcagctc tgggggccgg ggtgggcttg ttgggtgagg ctgagcggct 
     1621 ggtgtggttg gcggaggtgg ggattcccgt acctcgtgtt gtggagggtg gtggggacga 
     1681 gagggtcgcc tggttggtca ccgaagcggt tccggggcgt ccggccagtg cgcggtggcc 
     1741 gcgggagcag cggctggacg tggcggtggc gctcgcgggg ctcgctcgtt cgctgcacgc 
     1801 gctggactgg gagcggtgtc cgttcgatcg cagtctcgcg gtgacggtgc cgcaggcggc 
     1861 ccgtgctgtc gctgaaggga gcgtcgactt ggaggatctg gacgaggagc ggaaggggtg 
     1921 gtcgggggag cggcttctcg ccgagctgga gcggactcgg cctgcggacg aggatctggc 
     1981 ggtttgccac ggtcacctgt gcccggacaa cgtgctgctc gaccctcgta cctgcgaggt 
     2041 gaccgggctg atcgacgtgg ggcgggtcgg ccgtgcggac cggcactccg atctcgcgct 
     2101 ggtgctgcgc gagctggccc acgaggagga cccgtggttc gggccggagt gttccgcggc 
     2161 gttcctgcgg gagtacgggc gcgggtggga tggggcggta tcggaggaaa agctggcgtt 
     2221 ttaccggctg ttggacgagt tcttctgagg gacctgatgg tgttggtggc tgggtagggt 
     2281 tgcgtcgcgt gggtgacagc acagtgtgga cgttgggatc cccgctccgt gtaaatggag 
     2341 gcgctcgttg atctgagcct tgccccctga cgaacggcgg tggatggaag atactgctct 
     2401 caagtgctga agcggtagct tagctccccg tttcgtgctg atcagtcttt ttcaacacgt 
     2461 aaaaagcgga ggagttttgc aattttgttg gttgtaacga tcctccgttg attttggcct 
     2521 ctttctccat gggcgggctg ggcgtatttg aagcgggtac ccagcttttg ttccctttag 
     2581 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     2641 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
     2701 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
     2761 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     2821 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     2881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     2941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     3001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     3061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     3121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     3181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     3241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     3301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     3361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     3421 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     3481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     3541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     3601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     3661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     3721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     3781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     3841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     3901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     3961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     4021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     4081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     4141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     4201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     4261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     4321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     4381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     4441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     4501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     4561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     4621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     4681 ctcatgagcg g 
//
