LOCUS       pTol2pA2-ubb:sec       11166 bp    DNA     circular UNA 11-MAY-2017
DEFINITION  an expression clone created from pDESTtol2pACrymCherry and Reaction
            of 3 fragments.
ACCESSION   urn.local...1o-7k0spsk
VERSION     urn.local...1o-7k0spsk
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    2714..2735
                     /note="Geneious type: attB4"
     misc_feature    2742..6259
     misc_feature    6270..6291
                     /note="Geneious type: attP1"
     misc_feature    <6282..6284
     misc_recomb     <6282..6291
     misc_feature    6301..6357
     CDS             6358..7316
     misc_feature    complement(7318..7339)
                     /note="Geneious type: attB2"
     misc_recomb     complement(7319..>7322)
     CDS             7342..8061
     misc_feature    complement(8062..8083)
                     /note="Geneious type: attB3"
ORIGIN      
        1 dsttacrymc hrrystncbs ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
       61 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      121 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      181 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      241 tggcccacgc tggctaagaa ctcatcagcc tccccggtcc atctacccac gtaccaatgc
      301 accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt ataggaatgg
      361 agactacctc ctgtccaaca aggctcttgg atacgagtac gcctacctgt tggacccagg
      421 tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat ctgttcagac
      481 acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct tgacagaggt
      541 gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg aaaattttac
      601 tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa aaaagtactc
      661 cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag aaaactagag
      721 attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca aggttttatc
      781 ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc taatgccagt
      841 acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg attctcaaaa
      901 ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc
      961 ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag gctgcaaata
     1021 gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggcccattt acgccttaag
     1081 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1141 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1201 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1261 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
     1321 ttacttgtac agctcgtcca tgccgccggt ggagtggtgg ccctcggcgc gctcgtactg
     1381 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta
     1441 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta
     1501 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc
     1561 ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     1621 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     1681 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     1741 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     1801 gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg
     1861 gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     1921 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg
     1981 catgaactcc ttgatgatgg ccatgttgtc atcctcgccc ttgctcacca tggtggcgac
     2041 cggtaatgtc agacctggta actccttact gtaacttatt gcacctgatg aacaaaagat
     2101 gtgaactgaa cgcaagtagg aggctaatag aggctagtac agtacaaaga tccccaaatg
     2161 atgtgtgccg gccttagtgg aggcagaccc ctggttatat acctgtgctg cagaaagagg
     2221 ggcttacaaa tggatctctc tggaatggca ttatctcatg gtgggaacag tgttgtcagc
     2281 agaatcagcg gggtggactc tgacctgaga atgacagtct agcactgaat ggctcagaca
     2341 atactcttgt ggccaactat caccggaaaa taagtgcctg ccaaaacaaa ccagaccaga
     2401 tgaagctaca tggctgtaga acgtcagtat ttaatgtgtt gtggccactt ggttcaccag
     2461 atttaagagc taattttttt aaatatcaca gataatcaat cgcttttttt tttcttgact
     2521 gaaaagctta gactggaaaa ttctttgaaa aagtttgcac tacagaaaat aaatacataa
     2581 catcctgcac tgaatgcaca ctattaatag aaaagagagc attattgtag gaaatgtatt
     2641 gtatatgtac attctgtgca tttcacaatt ttctcgagca ggaaacagct atgaccatga
     2701 ttacgccaag ctatcaactt tgtatagaaa agttggctcc gaattcgccc ttaaaactcg
     2761 agaccagcaa agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat
     2821 tctgagtaag ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata
     2881 aatgataaca aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc
     2941 ctcaaacagt gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa
     3001 ttggttcaga agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc
     3061 tgattccaca atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac
     3121 agcactcact agctgcccta tatattttat tatattttac atatattatt ttatttattt
     3181 agctctgagt gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta
     3241 cctcaggctc agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct
     3301 tgttttcaac tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca
     3361 tgcgactttt ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat
     3421 ataattatat tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag
     3481 atagtcagta taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc
     3541 tatatataag tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa
     3601 tgtagaatga cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt
     3661 gaaaacatcc tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa
     3721 catatacaaa aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct
     3781 ggcagttctg tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca
     3841 gattccacaa gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac
     3901 tgcttgtaga ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat
     3961 atgataatta taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac
     4021 catagcaatc attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat
     4081 ctggtcatgt gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact
     4141 ctcgaccatc ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt
     4201 ttcaacgcaa atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta
     4261 aaaccatagc gtattacttt aattatagta aactttcgct ttctttatta caagagacgt
     4321 tttgtgttga ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac
     4381 cagtaacttg cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac
     4441 gtcaaatagg ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta
     4501 tttttaattt atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa
     4561 agaacccgga tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat
     4621 tttaaaaata attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa
     4681 atgaggaaat atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa
     4741 ctggagaaaa ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta
     4801 gatgttttat gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat
     4861 aacagtaacg ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg
     4921 taagctacga gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt
     4981 aaataaaggc tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag
     5041 aaaaaatata aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc
     5101 tttttgttcc tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc
     5161 caaatgtttc cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat
     5221 aataattttg gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca
     5281 tgcttcagga agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca
     5341 ttacaaacta ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc
     5401 aaatcaaaat ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag
     5461 aaattgtttt tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta
     5521 aaattccaaa atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa
     5581 cagtcttatc taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt
     5641 ttaaatgact gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc
     5701 ttactggccc caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca
     5761 ttattattct ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag
     5821 atgtgcagtt tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat
     5881 gatgcacaaa gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga
     5941 ccctaaattt tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata
     6001 gtccactgtc agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag
     6061 ttttctatta ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc
     6121 attttcgggt gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta
     6181 gaacattgta gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt
     6241 tacagggatc caaaaagggc gaattcgacc caagtttgta caaaaaagca ggctgccacc
     6301 atggtgcaca aggtgctgct ggccctgttc ttcatcttcc tggcccccgc cggcaccgca
     6361 caggttctca gaggcactgt gactgacttc cctggatttg atgagcgggc tgatgcagaa
     6421 actcttcgga aggctatgaa aggcttgggc acagatgagg agagcatcct gactctgttg
     6481 acatcccgaa gtaatgctca gcgccaggaa atctctgcag cttttaagac tctgtttggc
     6541 agggatcttc tggatgacct gaaatcagaa ctaactggaa aatttgaaaa attaattgtg
     6601 gctctgatga aaccctctcg gctttatgat gcttatgaac tgaaacatgc cttgaaggga
     6661 gctggaacaa atgaaaaagt actgacagaa attattgctt caaggacacc tgaagaactg
     6721 agagccatca aacaagttta tgaagaagaa tatggctcaa gcctggaaga tgacgtggtg
     6781 ggggacactt cagggtacta ccagcggatg ttggtggttc tccttcaggc taacagagac
     6841 cctgatgctg gaattgatga agctcaagtt gaacaagatg ctcaggcttt atttcaggct
     6901 ggagaactta aatgggggac agatgaagaa aagtttatca ccatctttgg aacacgaagt
     6961 gtgtctcatt tgagaaaggt gtttgacaag tacatgacta tatcaggatt tcaaattgag
     7021 gaaaccattg accgcgagac ttctggcaat ttagagcaac tactccttgc tgttgtgaaa
     7081 tctattcgaa gtatacctgc ctaccttgca gagaccctct attatgctat gaagggagct
     7141 gggacagatg atcataccct catcagagtc atggtttcca ggagtgagat tgatctgttt
     7201 aacatcagga aggagtttag gaagaatttt gccacctctc tttattccat gattaaggga
     7261 gatacatctg gggactataa gaaagctctt ctgctgctct gtggagaaga tgactaccca
     7321 gctttcttgt acaaagtggc tatggtgagc aagggcgagg agctgttcac cggggtggtg
     7381 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     7441 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     7501 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     7561 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     7621 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     7681 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     7741 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     7801 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     7861 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     7921 gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
     7981 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     8041 atggacgagc tgtacaagta accaacttta ttatacatag ttgataattc actggccgtc
     8101 gttttacggt accatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     8161 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     8221 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     8281 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     8341 atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc
     8401 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
     8461 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
     8521 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
     8581 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
     8641 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
     8701 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
     8761 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     8821 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     8881 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     8941 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     9001 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9061 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9121 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9181 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9241 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9841 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10141 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10261 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10321 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10381 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10441 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10561 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10621 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10681 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10741 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10861 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10921 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10981 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11161 aaagtg
//
