LOCUS       Exported                2291 bp ds-DNA     circular UNA 06-DEC-2015
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT016g_120415_pBR322_sgRNA-EGFP_HHR_cleaved
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          330
                     /label=-1
                     /note="-1"
     source          332..362
     source          332..362
     source          363..445
     source          363..445
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     primer_bind     complement(309..356)
                     /label=WT173
                     /note="WT173"
     misc_feature    332
                     /label=after cleavage starting site
                     /note="after cleavage starting site"
     primer_bind     343..362
                     /label=Target DNA
                     /note="Target DNA"
     primer_bind     347..362
                     /label=WT172
                     /note="WT172"
     misc_feature    complement(363..445)
                     /note="gRNA backbone"
     misc_feature    complement(527..1146)
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(1301..2161)
                     /note="amp marker"
     promoter        complement(2203..2231)
                     /note="amp prom"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gctggattcc acgggcacgg gcagcttgcc
      361 gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      421 gtggcaccga gtcggtgctt tttttaagct tgggccgctc gaggtacctc tctacatatg
      481 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      541 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      601 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      661 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      721 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      781 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      841 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      901 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      961 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1021 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1081 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1141 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1201 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1261 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1321 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1381 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1441 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1501 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1561 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1621 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1681 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1741 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1801 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1861 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1921 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1981 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2041 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2101 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2161 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2221 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2281 aagtgccacc t
//
