LOCUS       Exported                2352 bp ds-DNA     circular UNA 06-DEC-2015
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT016e_120115_pBR322_sgRNA-EGFP_dHHR_10_blocker
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          330
                     /label=-1
                     /note="-1"
     source          341..506
     source          341..506
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     primer_bind     complement(312..367)
                     /label=WT165
                     /note="WT165"
     misc_feature    331..340
                     /label=blocker
                     /note="blocker"
     misc_feature    complement(341..403)
                     /label=dHHR
                     /note="dHHR"
     primer_bind     355..420
                     /label=WT158
                     /note="WT158"
     modified_base   375
                     /label=mutation
                     /note="mutation"
     primer_bind     404..423
                     /label=Target DNA
                     /note="Target DNA"
     misc_feature    complement(424..506)
                     /note="gRNA backbone"
     misc_feature    complement(588..1207)
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(1362..2222)
                     /note="amp marker"
     promoter        complement(2264..2292)
                     /note="amp prom"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcccgtgccc ggtacatcca gctgatgagt
      361 cccaaatagg acgagacgcg cttcggtgcg tcctggattc cacgggcacg ggcagcttgc
      421 cgggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      481 agtggcaccg agtcggtgct ttttttaagc ttgggccgct cgaggtacct ctctacatat
      541 gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1021 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2341 aaagtgccac ct
//
