LOCUS       Exported                2359 bp ds-DNA     circular UNA 20-MAR-2017
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT016d_120115_pBR322_sgRNA-EGFP_dHHR_17_blocker
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     primer_bind     complement(312..367)
                     /label=WT164
                     /note="WT164"
     misc_feature    331..347
                     /label=blocker
                     /note="blocker"
     misc_feature    complement(348..410)
                     /label=dHHR
                     /note="dHHR"
     primer_bind     362..427
                     /label=WT158
                     /note="WT158"
     modified_base   382
                     /label=mutation
                     /note="mutation"
     primer_bind     411..430
                     /label=Target DNA
                     /note="Target DNA"
     misc_feature    complement(431..513)
                     /note="gRNA backbone"
     misc_feature    complement(595..1214)
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(1369..2229)
                     /note="amp marker"
     promoter        complement(2271..2299)
                     /note="amp prom"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcaagctgcc cgtgcccggt acatccagct
      361 gatgagtccc aaataggacg agacgcgctt cggtgcgtcc tggattccac gggcacgggc
      421 agcttgccgg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      481 ttgaaaaagt ggcaccgagt cggtgctttt tttaagcttg ggccgctcga ggtacctctc
      541 tacatatgac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1501 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2341 tccccgaaaa gtgccacct
//
