LOCUS       Exported                2376 bp ds-DNA     circular UNA 11-APR-2016
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT016l_041116_pBR322_sgRNA-EGFP_theophylline-dHHR_17_blocker
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          330
                     /label=-1
                     /note="-1"
     source          348..427
     source          348..427
     source          428..530
     source          428..530
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     misc_feature    331..347
                     /label=blocker
                     /note="blocker"
     primer_bind     complement(358..388)
                     /label=WT308
                     /note="WT308"
     modified_base   382
                     /label=inactive mutation
                     /note="inactive mutation"
     primer_bind     383..404
                     /label=WT307
                     /note="WT307"
     modified_base   384..414
                     /label=theophylline aptamer
                     /note="theophylline aptamer"
     primer_bind     428..447
                     /label=Target DNA
                     /note="Target DNA"
     misc_feature    complement(448..530)
                     /note="gRNA backbone"
     misc_feature    complement(612..1231)
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(1386..2246)
                     /note="amp marker"
     promoter        complement(2288..2316)
                     /note="amp prom"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcaagctgcc cgtgcccggt acatccagct
      361 gatgagtccc aaataggacg agatacatac cagccgaaag gcccttggca ggtgtcctgg
      421 attccacggg cacgggcagc ttgccgggtt ttagagctag aaatagcaag ttaaaataag
      481 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt aagcttgggc
      541 cgctcgaggt acctctctac atatgacatg tgagcaaaag gccagcaaaa ggccaggaac
      601 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      781 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1201 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1261 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1321 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1381 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1441 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1501 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1561 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1621 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1681 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1741 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1801 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1861 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1921 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1981 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2041 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2101 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2161 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2221 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2281 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2341 ggggttccgc gcacatttcc ccgaaaagtg ccacct
//
