LOCUS       Exported                2408 bp ds-DNA     circular UNA 02-MAY-2016
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT029d_042516_pBR322_sgRNA-EGFP_guanine-dHHR_17_blocker_M0
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          330
                     /label=-1
                     /note="-1"
     source          460..562
     source          460..562
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     misc_feature    331..347
                     /label=blocker
                     /note="blocker"
     primer_bind     complement(348..374)
                     /label=WT315
                     /note="WT315"
     primer_bind     complement(358..384)
                     /label=WT317
                     /note="WT317"
     primer_bind     371..399
                     /label=WT316
                     /note="WT316"
     primer_bind     378..406
                     /label=WT318
                     /note="WT318"
     modified_base   382
                     /label=dead mutation
                     /note="dead mutation"
     modified_base   384..386
     primer_bind     complement(424..445)
                     /label=WT264
                     /note="WT264"
     primer_bind     440..462
                     /label=WT265
                     /note="WT265"
     modified_base   444..446
     primer_bind     460..479
                     /label=Target DNA
                     /note="Target DNA"
     misc_feature    complement(480..562)
                     /note="gRNA backbone"
     misc_feature    complement(644..1263)
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     misc_feature    complement(1418..2278)
                     /note="amp marker"
     promoter        complement(2320..2348)
                     /note="amp prom"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcaagctgcc cgtgcccggt acatccagct
      361 gatgagtccc aaataggacg agatactata atcgcgtgga tatggcacgc aagtttctac
      421 cgggcaccgt aaatgtccga ctagtgtcct ggattccacg ggcacgggca gcttgccggg
      481 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      541 gcaccgagtc ggtgcttttt ttaagcttgg gccgctcgag gtacctctct acatatgaca
      601 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      661 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      721 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      781 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      841 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      901 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      961 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1021 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1081 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1141 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1201 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1261 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1321 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1381 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1441 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1501 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1561 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1621 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1681 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1741 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1801 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1861 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1921 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1981 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2041 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2101 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2161 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2221 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2281 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2341 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2401 tgccacct
//
