LOCUS       Exported                2410 bp ds-DNA     circular UNA 15-SEP-2016
DEFINITION  natural circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    pWT049e_091516_pBR322_sgRNA-mCherry_20_nt_spacer_guanine-dHHR_4-...
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, May 15, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          330
                     /label=-1
                     /note="-1"
     promoter        67..330
                     /label=hU6 prom
                     /note="hU6 prom promoter"
     misc_feature    complement(331..349)
     misc_feature    335
                     /label=T
                     /note="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    339
                     /label=G
                     /note="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    346
                     /label=G
                     /note="G"
                     /note="Geneious type: Editing History Replacement"
     modified_base   384
                     /label=dead mutation
                     /note="dead mutation"
     modified_base   386..388
     modified_base   446..448
     misc_feature    462..481
                     /label=protospacer
                     /note="protospacer"
     primer_bind     482..564
                     /label=Spy gRNA hairpins (constant)
                     /note="Spy gRNA hairpins (constant)"
     misc_feature    complement(646..1265)
                     /label=pBR322 origin
                     /note="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     primer_bind     complement(652..671)
                     /label=pBR322 end FP
                     /note="pBR322 end FP"
     misc_feature    complement(1420..2280)
                     /label=amp marker
                     /note="amp marker"
     promoter        complement(2322..2350)
                     /label=amp prom
                     /note="amp prom promoter"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc actgcgggat ggaggagacg gtacatccag
      361 ctgatgagtc ccaaatagga cgagatacta taatcgcgtg gatatggcac gcaagtttct
      421 accgggcacc gtaaatgtcc gactagtgtc ctggattcca cgtcccctcc accccacagt
      481 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      541 tggcaccgag tcggtgcttt ttttaagctt gggccgctcg aggtacctct ctacatatga
      601 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      661 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      721 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      781 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      841 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      901 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      961 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1021 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1081 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1141 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1201 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1261 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1321 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1381 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1441 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1501 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1561 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1621 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1681 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1741 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1801 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1861 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1921 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1981 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2041 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2101 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2161 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2221 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2281 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2341 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2401 agtgccacct
//
